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Amidation Bioinformatics

Amidation is a type of post-translational modification (PTM) in which an amide group (-NH2) is added to the C-terminal of an amino acid following translation. Amidation is an essential PTM for neuropeptides and hormones.

Top Research Reagents

We have 1934 products for the study of Amidation that can be applied to Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

Cholecystokinin Antibody (2753A) [Unconjugated]
MAB11040
CCK was detected in immersion fixed Capan‑2 human pancreatic adenocarcinoma cell line (positive staining) and Daudi human Burkitt's lymphoma cell line (negative staining) using Rabbit Anti-Human CCK Monoclonal Antibody (Catalog # MAB11040) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # <a class=NoLineLink href=CCK was detected in immersion fixed paraffin-embedded sections of human duodenum using Rabbit Anti-Human CCK Monoclonal Antibody (Catalog # MAB11040) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (<a class=NoLineLink href=

Rabbit Monoclonal
Species Human
Applications IHC, ICC

Neuropeptide Y Antibody
NBP1-46535
Immunohistochemistry: Neuropeptide Y Antibody [NBP1-46535] - Low magnification images showing the double staining pattern of NHE1 with neuropeptide NPY. Scale bar: 100um. Insets: High magnification images showing individual cells with double staining. Scale bar: 10um. Asterisks mark Hoechst-stained nuclei. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-019-42872-w), licensed under a CC-BY license.NHE1 distribution (A) and colocalisation with markers for specific cell types (B-D) and major inputs (E, F, G). (A) NHE1 immunoreactivity is distributed throughout the rostrocaudal axis of the SCN (encircled by the dotted lines). Scale bar: 200 um. OC: optic chiasm. 3 V: third ventricle. (B-G1) Low magnification images showing the double staining pattern of NHE1 with neuropeptides NP2 (B), GRP (C), and VIP (D) as well as markers for afferent inputs vGluT2 (E), NPY (F), and SERT (G1). Scale bar: 100 um. Insets: High magnification images showing individual cells with double staining. Scale bar: 10 um. Asterisks mark Hoechst-stained nuclei. (G2) High magnification image showing high degree of colocalisation (yellow) between NHE1 (green) and SERT (red). Scale bar: 10 um. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31015514), licensed under a CC-BY licence.

Goat Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

19 Publications
Glutaminyl-peptide Cyclotransferase/QPCT Antibody
NBP1-81838
Immunohistochemistry-Paraffin: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838] - Staining in human adrenal gland and liver tissues using anti-QPCT antibody. Corresponding QPCT RNA-seq data are presented for the same tissues.Western Blot: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838] - Analysis in human cell line SK-MEL-30.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, IHC, IHC-P

1 Publication
Transglutaminase 2/TGM2 Antibody
NBP1-86951
Immunohistochemistry-Paraffin: Transglutaminase 2/TGM2 Antibody [NBP1-86951] - Analysis in human placenta and testis tissues. Corresponding TGM2 RNA-seq data are presented for the same tissues.Immunohistochemistry-Paraffin: Transglutaminase 2/TGM2 Antibody [NBP1-86951] - Staining of human endometrium, fallopian tube, placenta and testis using Anti-TGM2 antibody NBP1-86951 (A) shows similar protein distribution across tissues to independent antibody NBP1-86952 (B).

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

2 Publications
Carboxypeptidase E/CPE Antibody
NBP2-15699
Western Blot: Carboxypeptidase E/CPE Antibody [NBP2-15699] - Analysis of CPE expression in transfected 293T cell line by CPE polyclonal antibody. A: Non-transfected lysate. B: CPE transfected lysate. 12% SDS PAGE gel, diluted at 1:500.Immunohistochemistry-Frozen: Carboxypeptidase E/CPE Antibody [NBP2-15699] - Representative dual-immunofluorescence staining of rat endothelial cell antigen-1 (RECA-1) and carboxypeptidase E (CPE) in rat heart frozen sections. Image from verified customer review.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

     1 Review

1 Publication
POMC Antibody
NB100-1533
Immunohistochemistry: POMC Antibody [NB100-1533] - Representative confocal images of POMC in POMC-transfected WT and Sel1L-/- N2a cells. White arrows point to POMC-containing secretory granules, while yellow arrows point to perinuclear POMC. KDEL marks the ER. Representative data from at least 2 independent experiments are shown. Image collected and cropped by CiteAb from the following publication (jci.org/articles/view/96420), licensed under a CC-BY license.Flow Cytometry: POMC Antibody [NB100-1533] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Goat Polyclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

10 Publications
Amylin Antibody
NBP2-33680
Immunohistochemistry-Paraffin: Amylin Antibody [NBP2-33680] - Staining of human placenta shows no positivity in trophoblastic cells as expected.Immunohistochemistry: Amylin Antibody [NBP2-33680] - Staining of human pancreas shows distinct cytoplasmic positivity in islets of Langerhans.

Rabbit Polyclonal
Species Human
Applications IHC, IHC-P

Thyrotropin Releasing Hormone Antibody
NBP2-34014
Immunohistochemistry-Paraffin: Thyrotropin Releasing Hormone Antibody [NBP2-34014] - Analysis in human cervix, uterine and skeletal muscle tissues. Corresponding TRH RNA-seq data are presented for the same tissues.Immunohistochemistry-Paraffin: Thyrotropin Releasing Hormone Antibody [NBP2-34014] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Rabbit Polyclonal
Species Human
Applications IHC, IHC-P

Peptidylglycine alpha-Amidating Monooxygenase/PAM Antibody
AF4837

Goat Polyclonal
Species Human
Applications WB

1 Publication
Adrenomedullin/ADM Antibody [Unconjugated]
AF6108
Adrenomedullin/ADM was detected in immersion fixed paraffin-embedded sections of human heart using Goat Anti-Human Adrenomedullin/ADM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6108) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # <a class=

Goat Polyclonal
Species Human
Applications IHC

1 Publication
Insulin Antibody (182410) [Unconjugated]
MAB1417
Insulin was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using Human/Mouse/Bovine Insulin Monoclonal Antibody (Catalog # MAB1417) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # <a class=Insulin was detected in immersion fixed paraffin-embedded sections of human pancreas using Rat Anti-Human/Mouse/Bovine Insulin Monoclonal Antibody (Catalog # MAB1417) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (<a class=

Rat Monoclonal
Species Human, Mouse, Bovine
Applications IHC, CyTOF-ready, ICC

24 Publications
PACAP/ADCYAP1 Antibody [Unconjugated]
AF6380
Western blot shows lysates of mouse kidney tissue. PVDF Membrane was probed with 1 µg/mL of Human PACAP/ADCYAP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6380) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=

Sheep Polyclonal
Species Mouse
Applications WB

1 Publication
Substance P [Biotin]
KGE007
N/A Substance P [Biotin]N/A Substance P [Biotin]


Species Multi-Species
Applications ELISA

34 Publications
VIP Antibody (OTI1E8)
NBP2-46349
Western Blot: VIP Antibody (1E8) [NBP2-46349] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VIP.Immunohistochemistry: VIP Antibody (1E8) [NBP2-46349] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Mouse Monoclonal
Species Human
Applications WB, IHC

Proprotein Convertase 1/PCSK1 Antibody (3D2)
H00005122-M02
Western Blot: Proprotein Convertase 1/PCSK1 Antibody (3D2) [H00005122-M02] - PCSK1 monoclonal antibody (M02), clone 3D2. Analysis of PCSK1 expression in Raw 264.7.Immunocytochemistry/Immunofluorescence: Proprotein Convertase 1/PCSK1 Antibody (3D2) [H00005122-M02] - Analysis of monoclonal antibody to PCSK1 on HeLa cell. Antibody concentration 30 ug/ml.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, ICC/IF

1 Publication
Cholecystokinin-B R/CCKBR Antibody
NB100-2803
Western Blot: Cholecystokinin-B R/CCKBR Antibody [NB100-2803] - (0.5ug/ml) staining of Mouse Pancreas lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.Immunohistochemistry: Cholecystokinin-B R/CCKBR Antibody [NB100-2803] - Negative Control showing staining of paraffin embedded Human Pancreas, with no primary antibody.

Goat Polyclonal
Species Human, Mouse, Rat
Applications WB, IHC, IHC-P

2 Publications
MYCBP2 Antibody
NB100-97841
Western Blot: MYCBP2 Antibody [NB100-97841] - Whole cell lysate (5, 15, 50 ug loaded) from HeLa cells probed with anti-MYCBP2 antibody at 0.04 ug/ml.

Rabbit Polyclonal
Species Human
Applications IP (-), WB

Procalcitonin Antibody (44D9)
NB120-14817

Mouse Monoclonal
Species Human, Rat
Applications WB, ICC/IF, IHC

     1 Review

11 Publications
GHRH Antibody - Azide and BSA Free
NBP3-04850
Western Blot: GHRH Antibody [NBP3-04850] - Western blot analysis of extracts of various cell lines, using GHRH antibody (NBP3-04850) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF

Gastrin Antibody
NBP3-12223
Immunohistochemistry-Paraffin: Gastrin Antibody [NBP3-12223] - Rat stomach-gastrin-1:50 dilution in IHC blocking buffer. DAB (brown) staining and hematoxylin QS (blue) counterstain. 10X and 20X magnification on Leica DM4000. Gastrin Antibody [NBP3-12223] - Rat stomach-gastrin-1:50 dilution in IHC blocking buffer. DAB (brown) staining and hematoxylin QS (blue) counterstain. 10X and 20X magnification on Leica DM4000.

Rabbit Polyclonal
Species Human, Rat, Primate
Applications WB, ELISA, ICC/IF