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Midbrain Development Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Midbrain Development Pathway and Nervousness, Parkinson Disease, Stenosis, Hydrocephalus, Dementia. The study of the Midbrain Development Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Midbrain Development Pathway has been researched in relation to Brain Development, Cerebellum Development, Cell Proliferation, Neuron Development, Neurogenesis. The Midbrain Development Pathway complements our catalog of research reagents including antibodies and ELISA kits against FGF8, OTX2, WNT, DMBX1, FGF18.

Top Research Reagents

We have 1245 products for the study of the Midbrain Development Pathway that can be applied to Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

Aromatase Antibody
NB100-1596
Western Blot: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) and hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase. Image collected and cropped by CiteAb from the following publication (https://www.hindawi.com/journals/bmri/2017/3795950/), licensed under a CC-BY license.Immunocytochemistry/Immunofluorescence: Aromatase Antibody [NB100-1596] - U2OS cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Aromatase Antibody NB100-1596 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, ICC/IF

16 Publications
Wnt-1 Antibody (10C8) - BSA Free
NBP1-51575
Western Blot: Wnt-1 Antibody (10C8) [NBP1-51575] - Analysis using WNT1 mouse mAb against NIH/3T3 (1), 3T3L1 (2) and Hela (3) cell lysate.Immunocytochemistry/Immunofluorescence: Wnt-1 Antibody (10C8) [NBP1-51575] - Confocal immunofluorescence analysis of Hela (left) and 3T3-L1 (right) cells using WNT1 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

Mouse Monoclonal
Species Human, Mouse
Applications WB, ELISA, Flow

2 Publications
OTX3 Antibody
NBP1-80255
Western Blot: OTX3 Antibody [NBP1-80255] - Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Positive Control: Human brainImmunohistochemistry-Paraffin: OTX3 Antibody [NBP1-80255] - Mouse Brain tissue, 4-8ug/ml.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, IHC, IHC-P

ELSPBP1 Antibody
NBP2-13957
Immunohistochemistry-Paraffin: ELSPBP1 Antibody [NBP2-13957] - Analysis in human epididymis and prostate tissues. Corresponding ELSPBP1 RNA-seq data are presented for the same tissues.Immunohistochemistry-Paraffin: ELSPBP1 Antibody [NBP2-13957] - Staining of human cerebral cortex, epididymis, prostate and testis using Anti-ELSPBP1 antibody NBP2-13957 (A) shows similar protein distribution across tissues to independent antibody NBP2-13958 (B).

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

Ephrin-B1 Antibody [Unconjugated]
AF473
Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Ephrin-B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF473) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=Ephrin-B1 was detected in immersion fixed frozen sections of mouse kidney using Goat Anti-Mouse Ephrin-B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF473) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

Goat Polyclonal
Species Mouse
Applications WB, Simple Western, IHC

32 Publications
Otx2 Antibody [Unconjugated]
AF1979
Western blot shows lysates of IMR-32 human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=Otx2 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line using Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow, upper panel; Catalog # <a class=

Goat Polyclonal
Species Human
Applications WB, Simple Western, IHC

     2 Reviews

165 Publications
Pax2 Antibody [Unconjugated]
AF3364
Western blot shows lysates of mouse fetal kidney. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Pax2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3364) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=Pax2 was detected in immersion fixed BG01V human embryonic stem cells differentiated into the early otic lineage using Goat Anti-Human Pax2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3364) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; <a class=

Goat Polyclonal
Species Human
Applications WB, IHC, ICC

24 Publications
Nurr1/NGFI-B beta/NR4A2 Antibody [Unconjugated]
AF2156
Nurr1/NGFI‑B beta /NR4A2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse Nurr1/NGFI‑B beta /NR4A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2156) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; <a class=NoLineLink href=Nurr1/NGFI‑B beta /NR4A2 was detected in immersion fixed paraffin-embedded sections of mouse brain (hippocampus) using Goat Anti-Human/Mouse Nurr1/NGFI‑B beta /NR4A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2156) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (<a class=NoLineLink href=

Goat Polyclonal
Species Human, Mouse
Applications WB, IHC, ICC

19 Publications
PACAP/ADCYAP1 Antibody [Unconjugated]
AF6380
Western blot shows lysates of mouse kidney tissue. PVDF Membrane was probed with 1 µg/mL of Human PACAP/ADCYAP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6380) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=

Sheep Polyclonal
Species Mouse
Applications WB

1 Publication
FGF-17 Antibody (121630)
MAB319
FGF‑17 was detected in immersion fixed paraffin-embedded sections of human kidney using Human FGF‑17 Monoclonal Antibody (Catalog # MAB319) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

Mouse Monoclonal
Species Human
Applications WB, IHC

2 Publications
LMX1A Antibody - BSA Free
NBP2-41193
Western Blot: LMX1A Antibody [NBP2-41193] - Western blot analysis of LMX1A in rat brain tissue lysate with LMX1A antibody at (A) 1 and (B) 2 ug/mL.Immunocytochemistry/Immunofluorescence: LMX1A Antibody [NBP2-41193] - Staining of LMX1A in mouse brain tissue with LMX1A Antibodyat 20 ug/mL.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ELISA, ICC/IF

2 Publications
FGF-4 [Unconjugated]
7460-F4/CF


Species Human
Applications BA

2 Publications
Sonic Hedgehog/Shh [Unconjugated]
464-SH
Recombinant Mouse Sonic Hedgehog/Shh (C25Il), N-Terminus (Catalog # 464-SH) induces alkaline phosphatase production by the C3H10T1/2 mouse embryonic fibroblast cell line. The activity is more than 30-fold greater than the top competitor's Sonic Hedgehog.Recombinant Mouse Sonic Hedgehog/Shh (C25II), N-Terminus (Catalog # 464-SH) has a molecular weight (MW) of 20.7 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).


Species Mouse
Applications BA

65 Publications
FGF-8 [Unconjugated]
423-F8
Recombinant Human/Mouse FGF-8b (Catalog # 423-F8) stimulates cell proliferation in the NR6R‑3T3 mouse fibroblast cell line. The ED<sub>50</sub> for this effect is 6.5-40 ng/mL in the presence of 1 μg/mL heparin.1 μg/lane of Recombinant Human/Mouse FGF-8b was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 23 kDa.


Species Human, Mouse
Applications BA

     1 Review

62 Publications
EN1/Engrailed 1 Antibody (3H2)
H00002019-M04
Immunohistochemistry-Paraffin: EN1/Engrailed 1 Antibody (3H2) [H00002019-M04] - Analysis of monoclonal antibody to EN1 on formalin-fixed paraffin-embedded human cerebellum. Antibody concentration 3 ug/mlELISA: EN1/Engrailed 1 Antibody (3H2) [H00002019-M04] - Detection limit for recombinant GST tagged EN1 is approximately 1ng/ml as a capture antibody.

Mouse Monoclonal
Species Human, Mouse
Applications WB, ELISA, IHC

1 Publication
FGF18 [Unconjugated]
8988-F18
Recombinant Human FGF-18 (Catalog # 8988-F18) induces cell proliferation of the Baf3mouse pro-B cells transfected with human FGF RIIIc. The ED<sub>50</sub>for this effect is 4-24 ng/mL.


Species Human
Applications BA

3 Publications
Pax5/BSAP Antibody (1207C) [Unconjugated]
MAB3487
Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Ramos human Burkitt's lymphoma cell line, Daudi human Burkitt's lymphoma cell line, and Nalm-6 human Pre-B acute lymphocytic leukemia cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse Pax5/BSAP Monoclonal Antibody (Catalog # MAB3487) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (<a class=Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (<a class=

Rabbit Monoclonal
Species Human, Mouse
Applications WB, Simple Western, Flow

1 Publication
GBX2 Antibody
NB100-55393
Immunocytochemistry/Immunofluorescence: GBX2 Antibody [NB100-55393] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).Immunohistochemistry-Paraffin: GBX2 Antibody [NB100-55393] - Staining of paraffin embedded Human Cortex. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Goat Polyclonal
Species Human, Mouse
Applications WB, ICC/IF, IHC

3 Publications