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Male Sex Differentiation Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Male Sex Differentiation Pathway and Disorders Of Sex Development, Testicular Feminization, Congenital Abnormality, Gonadal Dysgenesis, Virilism. The study of the Male Sex Differentiation Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Male Sex Differentiation Pathway has been researched in relation to Sex Differentiation, Sex Determination, Cell Differentiation, Secretion, Spermatogenesis. The Male Sex Differentiation Pathway complements our catalog of research reagents including antibodies and ELISA kits against SRY, NR5A1, AR, AMH, FDXR.

Top Research Reagents

We have 1773 products for the study of the Male Sex Differentiation Pathway that can be applied to Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

BRD2 Antibody
NBP1-30475
Western Blot: BRD2 Antibody [NBP1-30475] - Whole cell lysate (50 ug) from NIH 3T3 and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD2 antibody used for WB at 0.1 ug/ml. Detection:Chemiluminescence with an exposure time of 30 seconds.Immunocytochemistry/Immunofluorescence: BRD2 Antibody [NBP1-30475] - Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti- BRD2 used at a dilution of 1:100 (2 ug/mL) (left) and rabbit anti-BRD2 recombinant monoclonal used at a dilution of 1:20 (right). Detection: Red-fluorescent goat anti-rabbit IgG cross-adsorbed Antibody DyLight 594 conjugated.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ICC/IF, IHC

     1 Review

3 Publications
Aromatase Antibody
NB100-1596
Western Blot: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) and hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase. Image collected and cropped by CiteAb from the following publication (https://www.hindawi.com/journals/bmri/2017/3795950/), licensed under a CC-BY license.Immunocytochemistry/Immunofluorescence: Aromatase Antibody [NB100-1596] - U2OS cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Aromatase Antibody NB100-1596 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, ICC/IF

15 Publications
SF-1/NR5A1/Steroidogenic Factor 1 Antibody
NBP1-52823
Western Blot: SF-1/NR5A1/Steroidogenic Factor 1 Antibody [NBP1-52823] - Titration: 0.2-1 ug/ml, Positive Control: THP-1 cell lysate.Immunohistochemistry-Paraffin: SF-1/NR5A1/Steroidogenic Factor 1 Antibody [NBP1-52823] - Human adrenal tissue at an antibody concentration of 4-8ug/ml.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, Simple Western, ICC/IF

4 Publications
INSL3 Antibody
NBP1-81223
Immunohistochemistry-Paraffin: INSL3 Antibody [NBP1-81223] - Staining in human testis and endometrium tissues using anti-INSL3 antibody. Corresponding INSL3 RNA-seq data are presented for the same tissues.Immunohistochemistry-Paraffin: INSL3 Antibody [NBP1-81223] - Staining of human testis shows high expression.

Rabbit Polyclonal
Species Human
Applications ICC/IF, IHC, IHC-P

6 Publications
AKR1B1 Antibody
NBP1-89146
Immunocytochemistry/Immunofluorescence: AKR1B1 Antibody [NBP1-89146] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.Immunohistochemistry-Paraffin: AKR1B1 Antibody [NBP1-89146] - Staining of human liver shows no positivity in hepatocytes as expected.

Rabbit Polyclonal
Species Human
Applications ICC/IF, IHC, IHC-P

DBT Antibody
NBP1-89522
Immunohistochemistry-Paraffin: DBT Antibody [NBP1-89522] - Analysis in human kidney and pancreas tissues. Corresponding DBT RNA-seq data are presented for the same tissues.Immunohistochemistry-Paraffin: DBT Antibody [NBP1-89522] - Staining of human colon, liver, lymph node and pancreas using Anti-DBT antibody NBP1-89522 (A) shows similar protein distribution across tissues to independent antibody NBP1-85964 (B).

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

Androgen R/NR3C4 [p Ser213, p Ser210] Antibody (156C135.2) - BSA Free
NB100-56603
Western Blot: Androgen R/NR3C4 [p Ser213, p Ser210] Antibody (156C135.2) [NB100-56603] - Analysis using Azide Free version of NB100-56603. LNCaP cells (passage number 38) were serum-starved for 2 days. After serum starvation, cells were (A) left untreated, (B) treated with 100 ng/ml IGF-1 for 4h, or (C) incubated with 20 um LY294002 for 30 mi

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, IHC, IHC-P

14 Publications
GATA-4 Antibody - BSA Free
NBP2-24585
Western Blot: GATA-4 Antibody [NBP2-24585] - Analysis of GATA-4 in human kidney lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.Immunohistochemistry-Paraffin: GATA-4 Antibody [NBP2-24585] - Analysis of a FFPE tissue section of human kidney using 1:200 dilution of GATA-4 antibody (NBP2-24585). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, IHC, IHC-P

     1 Review

2 Publications
MIS RII/AMHR2 Antibody
AF1618
GnRH down-regulates AMH receptivity in vitro in L beta T2 cells and in vivo in pituitary of female rats at pnd 18.(a) GnRH inhibits AMH signaling. L beta T2 cells were stimulated for 24 h with 2.5 μg/ml AMH, 10 nM GnRHa or with a combination of both hormones. P-Smad1/5/8 protein level was evaluated by immunoblotting and normalized with total Smad1. *P ≤ 0.05 compared with AMH-stimulated cells. (b) Combined effects of GnRH and AMH on Fshb expression. Cells were incubated with 2.5 μg/ml AMH, 10 nM GnRHa or with both hormones for 24 h. Fshb mRNA levels were analyzed by real-time qPCR and expressed as percentage of control levels. *P ≤ 0.05 ; **P ≤ 0.01 compared with control cells. (c) GnRH down-regulates Amhr2 expression. Cells were incubated with 10 nM GnRHa, 10 ng/ml activin A or 20 ng/ml BMP2 for 4 and 24 h. Amhr2 mRNA levels were determined by real-time qPCR and expressed as percentage of control cells. Results are the mean ± SEM of 8 independent experiments. *P ≤ 0.05 compared with control cells. (d) GnRH decreases AMHR2 protein level. Cells were treated for 24 h with 10 nM GnRHa and AMHR2 protein level was determined by immunoblotting after normalization with vinculin. Results are the mean ± SEM of 4 independent experiments. *P ≤ 0.05 compared with control cells. (e) GnRH down-regulates Amhr2 expression in pituitary of female rats at pnd 18. Male and female rats were injected subcutaneously at pnd 17 with 100 μl of saline solution containing or not 0.1 μg of GnRHa. Anterior pituitary Amhr2 expression was determined 24 h after injection by Taqman real time qPCR. Each value is a mean ± SEM of 8 to 14 rats. *P ≤ 0.05 compared to control rats; a, P ≤ 0.01 compared to female control rats. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep23790), licensed under a CC-BY license. Not internally tested by R&D Systems.

Goat Polyclonal
Species Rat
Applications WB, IHC, Block

5 Publications
Amphiregulin Antibody
AF262
Western blot shows conditioned media from T47D human breast cancer cell line untreated (-) or treated (+) with PMA and PHA for 3 days. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Amphiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF262) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=Amphiregulin was detected in immersion fixed MCF-7 human breast cancer cell line using Goat Anti-Human Amphiregulin Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF262) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # <a class=

Goat Polyclonal
Species Human
Applications WB, IHC, ICC

     1 Review

35 Publications
SOX9 Antibody [Unconjugated]
AF3075
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, KATO-III human gastric carcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, and Hep3B human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human SOX9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3075) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=SOX9 was detected in immersion fixed HEK293 human embryonic kidney cell line using 10 µg/mL Human SOX9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3075) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; <a class=

Goat Polyclonal
Species Human
Applications WB, Simple Western, ICC

     2 Reviews

145 Publications
DAX1/NR0B1 Antibody (H7431)
PP-H7431-00

Mouse Monoclonal
Species Human
Applications WB

2 Publications
SRY Antibody (1G4) - BSA Free
NBP2-37463
Western Blot: SRY Antibody (1G4) [NBP2-37463] - Analysis using SRY mouse mAb against NTERA-2 (1) cell lysate.Immunohistochemistry-Paraffin: SRY Antibody (1G4) [NBP2-37463] - Analysis of ovarian cancer tissues using SRY mouse mAb with DAB staining.

Mouse Monoclonal
Species Human
Applications WB, ELISA, Flow

Ferredoxin Reductase Antibody
NBP2-38530
Western Blot: Ferredoxin Reductase Antibody [NBP2-38530] - Analysis in human cell lines A-549 and U-251MG using anti-FDXR antibody. Corresponding FDXR RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.Immunohistochemistry-Paraffin: Ferredoxin Reductase Antibody [NBP2-38530] - Staining in human adrenal gland and lymph node tissues using NBP2-38530 antibody. Corresponding FDXR RNA-seq data are presented for the same tissues.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

NT-3 [Unconjugated]
267-N3


Species Human
Applications BA

72 Publications
MIS/AMH [Unconjugated]
1737-MS/CF


Species Human
Applications Bind

17 Publications
NGFI-B alpha/Nur77/NR4A1 Antibody
NB100-56745
Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745] - RNA-seq results were validated by Western blot analysis in WT and calpain-1 KO mice. Western blot images and quantifications showing the expression levels of NGFI-B alpha/Nur77/NR4A1 protein in three independent replicates, respectively. Dot plots were used for the quantification of the expression levels of protein compared to actin control in WT and calpain-1 KO mice. Unpaired t-test in Prism 7 was used to calculate p values, *p < 0.05, **p < 0.01, ***p < 0.001. Image collected and cropped by CiteAb from the following publication (https://www.frontiersin.org/article/10.3389/fgene.2020.00334/full), licensed under a CC-BY license.Immunohistochemistry-Paraffin: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745] - Analysis of a FFPE tissue section of mouse brain using 1:200 dilution of NGFI-B alpha/Nur77/NR4A1 antibody (NB100-56745). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ChIP, ICC/IF

     3 Reviews

30 Publications
SLC7A5/LAT1 Antibody (BU53) - BSA Free
NBP2-50465
Immunohistochemistry-Paraffin: SLC7A5/LAT1 Antibody (BU53) [NBP2-50465] - Analysis of a FFPE tissue section of human testis using 1:200 dilution of SLC7A5/LAT1 (BU53) antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. Flow (Cell Surface): SLC7A5/LAT1 Antibody (BU53) [NBP2-50465] - A cell surface stain was performed on Raji cells with SLC7A5 (BU53) antibody NBP2-50465AF647 (blue) along with a matched isotype control NB600-986AF647 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at RT. Both antibodies were conjugated to Alexa Fluor 647.

Mouse Monoclonal
Species Human
Applications Flow, IHC, IHC-P

3 Publications