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Kinetochore Localization Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Kinetochore Localization Pathway and Malignant Neoplasms, Aneuploidy, Neoplasms, Cell Transformation, Neoplastic, Misalignment. The study of the Kinetochore Localization Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Kinetochore Localization Pathway has been researched in relation to Localization, Mitosis, Anaphase, Spindle Checkpoint, Chromosome Segregation. The Kinetochore Localization Pathway complements our catalog of research reagents including antibodies and ELISA kits against MAD2L1, MXI1, BUB1, MAD1L1, NDC80.

Top Research Reagents

We have 1174 products for the study of the Kinetochore Localization Pathway that can be applied to Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

MAD3 Antibody (S07-6H7)
NBP3-19989
Western Blot: MAD3 Antibody (S07-6H7) [NBP3-19989] - Western blot detection of MAD3 in Hela, A549, HL-60 using MAD3 Rabbit mAb (1:1000 diluted)

Rabbit Monoclonal
Species Human, Mouse, Rat
Applications WB

Bub1 Antibody
H00000699-D01P
Western Blot: Bub1 Antibody [H00000699-D01P] - Analysis of BUB1 expression in mouse testis.Immunocytochemistry/Immunofluorescence: Bub1 Antibody [H00000699-D01P] - Analysis of antibody to BUB1 on HeLa cell. Antibody concentration 10 ug/ml.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ICC/IF, PLA

1 Publication
MAD1L1/MAD1 Antibody
NBP1-32775
Western Blot: MAD1L1/MAD1 Antibody [NBP1-32775] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAD1 antibody diluted by 1:5000.Immunocytochemistry/Immunofluorescence: MAD1L1/MAD1 Antibody [NBP1-32775] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MAD1 protein stained by MAD1 antibody diluted at 1:500. Red: HEC1, a kinetochore marker, stained by HEC1 antibody [9G3.23] diluted at 1:500. Blue: Hoechst 33342 staining.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

2 Publications
NDC80 Antibody (9G3.23)
NB100-338
Western Blot: NDC80 Antibody (9G3.23) [NB100-338] - HEC1 antibody [9G3.23] detects HEC1 protein by western blot analysis. Various whole cell extracts and HeLa nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HEC1 antibody [9G3.23] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-193821) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate. Western Blot: NDC80 Antibody (9G3.23) [NB100-338] - WB analysis of NDC80 in U2OS Whole Cell Lysate. Verified customer review from 1DegreeBio.

Mouse Monoclonal
Species Human, Mouse, Hamster
Applications WB, Flow, ICC/IF

     5 Reviews

48 Publications
PLK1 Antibody (OTI1D4)
NBP1-48291
Western Blot: PLK1 Antibody (OTI1D4) [NBP1-48291] - (Negative control: IP without adding anti-PLK1 antibody.). For each experiment, 500ul of DDK tagged PLK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug ofImmunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded human endometrium tissue using PLK1 antibody (OTI1D4). Primary antibody dilution 1:200. Image from verified customer review.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Flow, IHC

     1 Review

ADCY10 Antibody
NBP1-81765
Immunohistochemistry-Paraffin: ADCY10 Antibody [NBP1-81765] - Staining of human kidney shows strong positivity in tubules.Immunohistochemistry-Paraffin: ADCY10 Antibody [NBP1-81765] - Staining of human pancreas shows strong membranous positivity in subset of exocrine glandular cells.

Rabbit Polyclonal
Species Human
Applications IHC, IHC-P

ZWINT Antibody
NBP1-82868
Western Blot: ZWINT Antibody [NBP1-82868] - Analysis in control (vector only transfected HEK293T lysate) and ZWINT over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).Immunocytochemistry/Immunofluorescence: ZWINT Antibody [NBP1-82868] - Staining of human cell line RH-30 shows localization to nucleoplasm, nuclear bodies & cytosol.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

CENPE Antibody
NBP1-82875
Immunocytochemistry/Immunofluorescence: CENPE Antibody [NBP1-82875] - Staining of human cell line A-431 shows localization to cytosol & microtubules.Immunohistochemistry-Paraffin: CENPE Antibody [NBP1-82875] - Staining of human stomach, upper shows strong nuclear positivity in glandular cells.

Rabbit Polyclonal
Species Human
Applications ICC/IF, IHC, IHC-P

Bub3 Antibody
NBP1-88517
Western Blot: Bub3 Antibody [NBP1-88517] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-PPIB.Immunocytochemistry/Immunofluorescence: Bub3 Antibody [NBP1-88517] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ICC/IF, IHC

1 Publication
Cdc20 Antibody
NB100-59828
Western Blot: Cdc20 Antibody [NB100-59828] - Detection of Human and Mouse CDC20 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CDC20 antibody NB100-59828 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Immunohistochemistry-Paraffin: Cdc20 Antibody [NB100-59828] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CDC20 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB

Rabbit Polyclonal
Species Human, Mouse
Applications WB, IHC, IHC-P

5 Publications
BubR1 Antibody (8G1) - BSA Free
NB100-353
Western Blot: BubR1 Antibody (8G1) [NB100-353] - BUBR1 detected in HeLa cell lysate using NB 100-353.Immunocytochemistry/Immunofluorescence: BubR1 Antibody (8G1) [NB100-353] - Immunofluorescence of an asynchronous cycling population of human cells (U2OS) with NB100-353. No signal is detected from interphase cells, whereas cells undergoing mitosis accumulate BubR1 at the kinetochores. Image reveals kinetochores at prometaphase. Image courtesy of Luke Hughes-Davies and Rhiannon Jade, Gurdon Institute, Cambridge, UK.

Mouse Monoclonal
Species Human, Mouse
Applications WB, Flow, ICC/IF

     1 Review

8 Publications
MAD2L1 Antibody
NB100-563
Immunohistochemistry: MAD2L1 Antibody [NB100-563] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti- MAD2 used at a dilution of 1:1,000 (1ug/ml). Detection: DABImmunoprecipitation: MAD2L1 Antibody [NB100-563] - Detection of human MAD2L1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MAD2L1 antibody NB100-563 (lot 3) used for IP at 3 ug/mg lysate. MAD2L1 was also immunoprecipitated by a previous lot (lot 2) and by rabbit anti-MAD2L1 antibody NB100-564. Detection: Chemiluminescence with an exposure time of 30 seconds.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

     1 Review

14 Publications
Mxi1 Antibody [Unconjugated]
AF4185
Mxi1 was detected in immersion fixed LNCaP human prostate cancer cell line using Goat Anti-Human Mxi1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4185) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

Goat Polyclonal
Species Human
Applications ICC

1 Publication
TTK Antibody (689913)
MAB6028
Western blot shows lysates of A431 human epithelial carcinoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 2 µg/mL of Human TTK Monoclonal Antibody (Catalog # MAB6028) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

Mouse Monoclonal
Species Human
Applications WB

     1 Review

ZW10 Antibody
NBP2-38644
Western Blot: ZW10 Antibody [NBP2-38644] - Analysis using Anti-ZW10 antibody NBP2-38644 (A) shows similar pattern to independent antibody NBP2-38751 (B).Immunocytochemistry/Immunofluorescence: ZW10 Antibody [NBP2-38644] - Staining of human cell line U-2 OS shows localization to cytosol & endoplasmic reticulum. Antibody staining is shown in green.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

1 Publication
Smad3 Antibody
NB100-56479
Western Blot: Smad3 Antibody [NB100-56479] - Analysis in human fibroblast and HeLa whole cell lysates. Untreated lysates show a band around 55 kDa. 1:1000 dilution, 1 hour blocking in milk 5%. WB image submitted by a verified customer review.Immunocytochemistry/Immunofluorescence: Smad3 Antibody [NB100-56479] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-Smad3 at 10 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

     5 Reviews

8 Publications
Ribosomal protein S27 Antibody
NB100-1103
Western Blot: Ribosomal protein S27 Antibody [NB100-1103] - Staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry-Paraffin: Ribosomal protein S27 Antibody [NB100-1103] - Staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Goat Polyclonal
Species Human
Applications WB, IHC, IHC-P

1 Publication
Mad Antibody
NBP2-85249
Western Blot: Mad Antibody [NBP2-85249] - Host: Mouse. Target Name: MXD1. Sample Tissue: Mouse Small Intestine. Antibody Dilution: 1ug/mlWestern Blot: Mad Antibody [NBP2-85249] - WB Suggested Anti-MXD1 Antibody Titration: 0.2-1 ug/ml. Positive Control: Human Muscle

Rabbit Polyclonal
Species Human, Mouse
Applications WB

IkB-alpha Antibody (6A920) - BSA Free
NB100-56507
Knockout Validated: IkB-alpha Antibody (6A920) [NB100-56507] - Western blot shows lysates of HEK293 human embryonic kidney parental cell line and IkB-alpha knockout (KO) HEK293 human embryonic kidney cell line. PVDF membrane was probed with 2 ug/ml of Mouse Anti-Human IkB-alpha monoclonal Antibody (Catalog # NB100-56507) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody. Specific band was detected for IkB-alpha at approximately 38 kDa (as indicated) in the parental HEK293 celll line, but is not detectable in the knockout HEK293 cell line. This experiment was conducted under reducing conditions.Western Blot: IkB-alpha Antibody (6A920) [NB100-56507] - Lysates of Jurkat human acute T cell leukemia cell line, LNCaP human prostate cancer cell line, PCx2011;3 human prostate cancer cell line, HeLa human cervical epithelial carcinoma cell line, and NIHx2011;3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.5 ug/mL mouse anti-IkB-a monoclonal (NB100-56507, Novus Biologicals), followed by 1:2000 dilution of the appropriate HRP-conjugated secondary antibody, donkey anti-mouse IgG.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, Flow

42 Publications
CENPA Antibody
NBP3-13389
Western Blot: CENPA Antibody [NBP3-13389] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with CENPA antibody (NBP3-13389) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Immunohistochemistry-Paraffin: CENPA Antibody [NBP3-13389] - Human lung cancer. CENPA stained by CENPA antibody (NBP3-13389) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P