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Kinetochore Assembly Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Kinetochore Assembly Pathway and Aneuploidy, Malignant Neoplasms, Stenosis, Neoplasms, Instabilities, Chromosomal. The study of the Kinetochore Assembly Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Kinetochore Assembly Pathway has been researched in relation to Chromosome Segregation, Mitosis, Localization, Cell Cycle, Interphase. The Kinetochore Assembly Pathway complements our catalog of research reagents including antibodies and ELISA kits against CENPA, CENPC1, NDC80, SUGT1, MIS12.

Top Research Reagents

We have 740 products for the study of the Kinetochore Assembly Pathway that can be applied to Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

CENPI Antibody
NBP3-24732
Staining of human cell line SiHa shows localization to nucleoplasm & nuclear bodies.Analysis in human cell line RT-4 and human cell line U-251 MG.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF

CENPF Antibody - BSA Free
NB500-101
Knockdown Validated: CENPF Antibody - BSA Free [NB500-101] - Kinetochore localization of RZZ and MAD1 are independent of CENP-E and CENP-F. CENP-F depletion does not interfere with the recruitment of Zwilch. The graphs show mean intensity of one (B, C, and E), two (D and F), or three (A, G, and H) experiments; the error bars indicate S.E., and the mean values for nondepleted cells are set to 1. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29748388/) licensed under a CC-BY license.Western Blot: CENPF Antibody [NB500-101] - 25ug of asynchronous and mitotically blocked HeLa lysates were loaded in each respective lane.

Rabbit Polyclonal
Species Human, Mouse, Bovine
Applications WB, Flow, ICC/IF

     2 Reviews

62 Publications
CENPB Antibody
H00001059-B01P
Western Blot: CENPB Antibody [H00001059-B01P] - Analysis of CENPB expression in A-431.Immunocytochemistry/Immunofluorescence: CENPB Antibody [H00001059-B01P] - Analysis of purified antibody to CENPB on HeLa cell. (antibody concentration 10 ug/ml)

Mouse Polyclonal
Species Human
Applications WB, ICC/IF

4 Publications
NDC80 Antibody (9G3.23)
NB100-338
Western Blot: NDC80 Antibody (9G3.23) [NB100-338] - HEC1 antibody [9G3.23] detects HEC1 protein by western blot analysis. Various whole cell extracts and HeLa nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HEC1 antibody [9G3.23] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-193821) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate. Western Blot: NDC80 Antibody (9G3.23) [NB100-338] - WB analysis of NDC80 in U2OS Whole Cell Lysate. Verified customer review from 1DegreeBio.

Mouse Monoclonal
Species Human, Mouse, Hamster
Applications WB, Flow, ICC/IF

     5 Reviews

48 Publications
CENPH Antibody
NBP1-82546
Western Blot: CENPH Antibody [NBP1-82546] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-119Immunocytochemistry/Immunofluorescence: CENPH Antibody [NBP1-82546] - Staining of human cell line A-431 shows localization to nucleus & nucleoli. Antibody staining is shown in green.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

2 Publications
CENPE Antibody
NBP1-82875
Immunocytochemistry/Immunofluorescence: CENPE Antibody [NBP1-82875] - Staining of human cell line A-431 shows localization to cytosol & microtubules.Immunohistochemistry-Paraffin: CENPE Antibody [NBP1-82875] - Staining of human stomach, upper shows strong nuclear positivity in glandular cells.

Rabbit Polyclonal
Species Human
Applications ICC/IF, IHC, IHC-P

BubR1 Antibody (8G1) - BSA Free
NB100-353
Western Blot: BubR1 Antibody (8G1) [NB100-353] - BUBR1 detected in HeLa cell lysate using NB 100-353.Immunocytochemistry/Immunofluorescence: BubR1 Antibody (8G1) [NB100-353] - Immunofluorescence of an asynchronous cycling population of human cells (U2OS) with NB100-353. No signal is detected from interphase cells, whereas cells undergoing mitosis accumulate BubR1 at the kinetochores. Image reveals kinetochores at prometaphase. Image courtesy of Luke Hughes-Davies and Rhiannon Jade, Gurdon Institute, Cambridge, UK.

Mouse Monoclonal
Species Human, Mouse
Applications WB, Flow, ICC/IF

     1 Review

8 Publications
MAD2L1 Antibody
NB100-563
Immunohistochemistry: MAD2L1 Antibody [NB100-563] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti- MAD2 used at a dilution of 1:1,000 (1ug/ml). Detection: DABImmunoprecipitation: MAD2L1 Antibody [NB100-563] - Detection of human MAD2L1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MAD2L1 antibody NB100-563 (lot 3) used for IP at 3 ug/mg lysate. MAD2L1 was also immunoprecipitated by a previous lot (lot 2) and by rabbit anti-MAD2L1 antibody NB100-564. Detection: Chemiluminescence with an exposure time of 30 seconds.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

     1 Review

14 Publications
Mxi1 Antibody [Unconjugated]
AF4185
Mxi1 was detected in immersion fixed LNCaP human prostate cancer cell line using Goat Anti-Human Mxi1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4185) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

Goat Polyclonal
Species Human
Applications ICC

1 Publication
MIS12 Antibody
NB100-2359
Western Blot: MIS12 Antibody [NB100-2359] - Detection of human MIS12 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MIS12 antibody NB100-2359 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.Immunohistochemistry: MIS12 Antibody [NB100-2359] - Detection of human MIS12 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-MIS12 (NB100-2359). Detection: DAB

Rabbit Polyclonal
Species Human
Applications WB, IHC, IP

2 Publications
SUGT1 Antibody (1A10)
H00010910-M04
Western Blot: SUGT1 Antibody (1A10) [H00010910-M04] - Analysis of SUGT1 expression in Raw 264.7 (Cat # L024V1).Western Blot: SUGT1 Antibody (1A10) [H00010910-M04] - Analysis of SUGT1 expression in PC-12 (Cat # L012V1).

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, IP

CNPase Antibody (CL2887)
NBP2-46617
Western Blot: CNPase Antibody (CL2887) [NBP2-46617] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissueImmunocytochemistry/Immunofluorescence: CNPase Antibody (CL2887) [NBP2-46617] - Staining of RH-30 cells using the Anti-CNP monoclonal antibody, showing specific staining in the cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

1 Publication
SGTA Antibody (2E11)
H00006449-M06
Western Blot: SGTA Antibody (2E11) [H00006449-M06] - SGTA expression in PC-12 ( Cat # L012V1 ).Immunocytochemistry/Immunofluorescence: SGTA Antibody (2E11.) [H00006449-M06] - SGTA Antibody (2E11) [H00006449-M06] - Analysis of monoclonal antibody to SGTA on HeLa cell. Antibody concentration 10 ug/ml

Mouse Monoclonal
Species Human
Applications WB, ELISA, ICC/IF

CENPC Antibody (JG36-15)
NBP2-75438
Western Blot: CENPC Antibody (JG36-15) [NBP2-75438] - Western blot analysis of CENPC on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HL-60 cell lysate Lane 2: Daudi cell lysate Lane 3: K562 cell lysateImmunocytochemistry/Immunofluorescence: CENPC Antibody (JG36-15) [NBP2-75438] - Staining CENPC in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Rabbit Monoclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

CENPA Antibody
NBP3-13389
Western Blot: CENPA Antibody [NBP3-13389] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with CENPA antibody (NBP3-13389) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Immunohistochemistry-Paraffin: CENPA Antibody [NBP3-13389] - Human lung cancer. CENPA stained by CENPA antibody (NBP3-13389) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

GCR2 Antibody (PCRP-ECD-1D10)
NBP3-13849
Immunocytochemistry/Immunofluorescence: GCR2 Antibody (PCRP-ECD-1D10) [NBP3-13849] - Immunofluorescence analysis of PFA-fixed HeLa cells. GCR2 Antibody (PCRP-ECD-1D10) followed by goat anti-mouse IgG-CF488 (green).Flow Cytometry: GCR2 Antibody (PCRP-ECD-1D10) [NBP3-13849] - Flow cytometric analysis of PFA-fixed HeLa cells. GCR2 antibody (PCRP-ECD-1D10) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).

Mouse Monoclonal
Species Human
Applications Flow, ICC/IF, IP