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Histone Methylation Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Histone Methylation Pathway and Malignant Neoplasms, Neoplasms, Cell Transformation, Neoplastic, Leukemia, Carcinogenesis. The study of the Histone Methylation Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Histone Methylation Pathway has been researched in relation to Methylation, Dna Methylation, Demethylation, Histone Acetylation, Gene Silencing. The Histone Methylation Pathway complements our catalog of research reagents including antibodies and ELISA kits against HISTONE H3, HIST1H4F, HIST2H4A, KDM1A, POLYCOMB.

Top Research Reagents

We have 891 products for the study of the Histone Methylation Pathway that can be applied to Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

H4/j Overexpression Lysate
NBL1-11567
Western Blot: H4/j Overexpression Lysate (Adult Normal) [NBL1-11567] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for H4/j.


Species Human
Applications WB

Recombinant Human H4/e GST (N-Term) Protein
H00008363-P01
12.5% SDS-PAGE Stained with Coomassie Blue.


Species Human
Applications WB, ELISA, PA

Recombinant Human H4/d GST (N-Term) Protein
H00008362-P01
12.5% SDS-PAGE Stained with Coomassie Blue.


Species Human
Applications WB, ELISA, PA

KMT1A/SUV39H1 Antibody
NBP1-21367
Western Blot: KMT1A/SUV39H1 Antibody [NBP1-21367] - Detection of human SUV39H1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SUV39H1 antibody NBP1-21367 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. SUV39H1 was also immunoprecipitated by a rabbit anti-SUV39H1 antibody recognizing a downstream epitope (B). Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).Immunohistochemistry-Paraffin: KMT1A/SUV39H1 Antibody [NBP1-21367] - SUV39H1 expression in human glioma tissue grade IV. Image submitted by a verified customer review.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ICC/IF, IHC

     4 Reviews

4 Publications
Histone H4 [ac Lys5] Antibody - BSA Free
NB21-2024
Western Blot: Histone H4 [ac Lys5] Antibody [NB21-2024] - Analysis of H4K5ac in NIH 3t3 histone prep.Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys5] Antibody [NB21-2024] - Histone H4 K5ac antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, DB, ICC/IF

     2 Reviews

4 Publications
CCDC6 Antibody
NBP1-85351
Western Blot: CCDC6 Antibody [NBP1-85351] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).Immunocytochemistry/Immunofluorescence: CCDC6 Antibody [NBP1-85351] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

HIST1H4F Overexpression Lysate
NBP2-07996
Western Blot: HIST1H4F Overexpression Lysate (Adult Normal) [NBP2-07996] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HIST1H4F.


Species Human
Applications WB

H4C4 Overexpression Lysate
NBP2-10888
Western Blot: H4C4 Overexpression Lysate [NBP2-10888] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for H4C4.


Species Human
Applications WB

Recombinant Human HIST1H4I GST (N-Term) Protein
H00008294-P01
SDS-Page: Recombinant Human HIST1H4I Protein [H00008294-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.


Species Human
Applications WB, ELISA, PA

EZH2/KMT6 Antibody [Unconjugated]
AF4767
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and mouse spleen tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse EZH2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4767) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=Measurement of EZH2 protein under conditions of SIRT1 knockdown. SIRT1 knockdown in Caco-2 cells was achieved using two different siRNAs and protein was analysed by Western blotting using anti-EZH2 antibody (a) or anti-SIRT1 antibody (to confirm efficacy of knockdown) (b). Blots were probed with anti-alpha -tubulin antibody to confirm equal protein loading and transfer. Approximately 10 μg of protein was loaded in each lane. Three biological replicates are presented for each condition. Approximate molecular weights are indicated Image collected and cropped by CiteAb from the following publication (https://humgenomics.biomedcentral.com/articles/10.1186/s40246-015-0036-0), licensed under a CC-BY license. Not internally tested by R&D Systems.

Goat Polyclonal
Species Human, Mouse
Applications WB, ICC

3 Publications
G9a/EHMT2 Antibody (A8620A) [Unconjugated]
PP-A8620A-00
G9a is not required for the transcriptional repression of selected early embryonic genes during retinoic acid (RA)-induced differentiation. The indicated mESC lines were untreated (0) or treated with 1 μM RA for 2, 4 or 8 days. (A) Immunoblots were used to determine G9a, Oct3/4 and GAPDH (loading control) protein levels in whole cell extracts. (B) Graph shows the messenger (mRNA) levels for the indicated genes using RT-qPCR analysis with Ubiquitin C (UBC) as the normalization control. All mRNA levels are normalized to the TT2 0 RA sample. Results shown are mean ± SD for three PCR reactions performed on the same cDNA sample and are representative of three independent experiments. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25478012), licensed under a CC-BY license. Not internally tested by R&D Systems.G9a expression is positively correlated with cell proliferation and poor prognosis in HNSCC patients. (A) Correlation between Ki-67 and G9a expression. The expression of two proteins were analyzed by IHC staining of tissue microarray (TMA) containing 46 normal and 108 tumor sections from HNSCC patients. The expression level of both Ki-67 and G9a was measured by relative staining intensity multiplied by percentage. Scale bar, 50 μm. (*, p < 0.05; ***, p < 0.001). (B) Ki-67 and G9a IHC staining of serial section of tumors. Low or high Ki-67 and G9a expression were defined by a threshold of 80 and 143, which is the average of relative staining intensity multiplied by percentage of Ki-67 and G9a. Scale bar, 50 μm. (***, p < 0.001). (C) Kaplan-Meier plot of overall survival of 77 HNSCC patients from a National Taiwan University Hospital cohort stratified by G9a expression level. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25027955), licensed under a CC-BY license. Not internally tested by R&D Systems.

Mouse Monoclonal
Species Human, Mouse
Applications WB, IHC, IP

16 Publications
Histamine H4R Antibody - BSA Free
NLS3775
Immunohistochemistry-Paraffin: Histamine H4R Antibody [NLS3775] - Lung, Non Small-Cell CarcinomaImmunohistochemistry-Paraffin: Histamine H4 R Antibody [NLS3775] - IHC analysis of Lymph node using this antibody at 12 ug/ ml.

Rabbit Polyclonal
Species Human, Monkey
Applications ICC/IF, IHC, IHC-P

1 Publication
LSD1 Antibody (1B2E5)
NB100-1762
Western Blot: LSD1 Antibody (1B2E5) [NB100-1762] - KDM1A is strongly overexpressed in human medulloblastomas, cell lines derived from them and murine medulloblastic tumors. Strong KDM1A protein expression was confirmed in the medulloblastic tumors from SmoA1-and Ptch+/A-mice relative to KDM1A expression in cerebellar tissue (CB) using western blotting of tissue lysates. I2-actin expression was used as a loading control. Image collected and cropped by Citeab from the following publication (The KDM1A histone demethylase is a promising new target for the epigenetic therapy of medulloblastoma. Acta Neuropathol Commun (2013)) licensed under a CC-BY license.Immunohistochemistry-Paraffin: LSD1 Antibody (1B2E5) [NB100-1762] - KDM1A is strongly overexpressed in human medulloblastomas, cell lines derived from them and murine medulloblastic tumors. KDM1A protein expresion was evaluated immunohistochemically in a tissue microarray of 70 medulloblastomas (MB) and 9 tissue samples of normal cerebellum (CB). Micrograph showing KDM1A-positive staining in a representative MB sample, and KDM1A-negative staining in CB, scale bar=100AI1/4m. Image collected and cropped by Citeab from the following publication (The KDM1A histone demethylase is a promising new target for the epigenetic therapy of medulloblastoma. <i>Acta Neuropathol Commun</i> (2013)) licensed under a CC-BY license.

Mouse Monoclonal
Species Human, Mouse, Primate
Applications WB, Simple Western, ELISA

12 Publications
H4/n Antibody (3E7H2) - BSA Free
NBP2-52486
Western Blot: H4/n Antibody (3E7H2) [NBP2-52486] - Analysis using HIST2H4A(20Me) mouse mAb against THP-1 (1) cell lysate.Immunocytochemistry/Immunofluorescence: H4/n Antibody (3E7H2) [NBP2-52486] - Analysis of HeLa cells using HIST2H4A(20Me) mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Mouse Monoclonal
Species Human
Applications WB, ELISA, Flow

DNMT1 Antibody (60B1220.1) - BSA Free
NB100-56519
Western Blot: DNMT1 Antibody (60B1220.1) [NB100-56519] - Analysis of Dnmt1 in 2102EP (human embryonic carcinoma) in the A) absence and B) presence of immunizing peptide, recombinant human Dnmt1 protein in the C) absence and D) presence of immunizing peptide, NIH 3T3 (mouse embryonic fribroblast) in the E) absence and F) presence of immunizing peptide, and D3 (mouse embryonic stem cell) in the G) absence and H) presence of immunizing peptide using Dnmt1 antibody. this antibody. 5 ug/ml for testing the human cell line and 0.1 ug/ml was used for testing the recombinant protein and the mouse cell lines. ’s goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.Immunocytochemistry/Immunofluorescence: DNMT1 Antibody (60B1220.1) [NB100-56519] - Dnmt1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Antibody dilution of 1:10 was used. Image objective 40x.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, Flow

     4 Reviews

140 Publications
KMT2A/MLL Antibody
NB600-248
Western Blot: KMT2A/MLL Antibody [NB600-248] - Samples: Nuclear extract (50 and 15 ug) from HeLa, 293T, and Jurkat cells. Antibody: Affinity purified rabbit anti-MLL1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Immunoprecipitation: KMT2A/MLL Antibody [NB600-248] - Samples: Nuclear Extract (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MLL1 antibody NB600-248 used for IP at 6 ug per reaction. MLL1 was also immunoprecipitated by rabbit anti-MLL1 antibody NB600-249. For blotting immunoprecipitated MLL1, NB600-248 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, IHC, IHC-P

     1 Review

28 Publications
MBD2 Antibody
NB100-55415
Western Blot: MBD2 Antibody [NB100-55415] - Staining of Jurkat cell lysate (35 ug protein in RIPA buffer) with antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry-Paraffin: MBD2 Antibody [NB100-55415] - Staining of paraffin embedded Human Skin with antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Goat Polyclonal
Species Human
Applications WB, IHC, IHC-P

2 Publications
H4/h Antibody (6D4)
H00008365-M01
Immunocytochemistry/Immunofluorescence: H4/h Antibody (6D4) [H00008365-M01] - Analysis of monoclonal antibody to HIST1H4H on HeLa cell . Antibody concentration 10 ug/ml.Sandwich ELISA: H4/h Antibody (6D4) [H00008365-M01] - Detection limit for recombinant GST tagged HIST1H4H is 0.1 ng/ml as a capture antibody.

Mouse Monoclonal
Species Human
Applications ELISA, ICC/IF, S-ELISA