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Diaphragm Development Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Diaphragm Development Pathway and Hernia, Diaphragmatic Hernia, Congenital Diaphragmatic Hernia, Congenital Abnormality, Hypoplasia. The study of the Diaphragm Development Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Diaphragm Development Pathway has been researched in relation to Pathogenesis, Lung Development, Cell Proliferation, Tissue Development, Cell Differentiation. The Diaphragm Development Pathway complements our catalog of research reagents including antibodies and ELISA kits against PAX-6, ASIC2, BNC1, EFNB1, FGF10.

Top Research Reagents

We have 1635 products for the study of the Diaphragm Development Pathway that can be applied to Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

MyoD Antibody (5.8A) - BSA Free
NB100-56511
Immunocytochemistry/Immunofluorescence: MyoD Antibody (5.8A) [NB100-56511] - Human myoblasts were stained with MyoD1 antibody, diluted 1:200. ICC/IF image submitted by a verified customer review.Western Blot: MyoD Antibody (5.8A) [NB100-56511] - Insuline-like growth factor (IGF1) antagonized the effects of miR-106a-5p on myogenic differentiation in C2C12. All data were collected from C2C12 myotubes 5 d post differentiation. Western-blot analysis of myogenic regulatory factors (MyoD, MyoG, MyHC) in cells; (H) The statistical results of Figure 3G. Data were presented as mean +/- SEM. n = 3 per group. * p < 0.05, ** p < 0.01. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/2073-4425/9/7/333/htm) licensed under a CC-BY license. anti-MyoG (catalog# NB100-56510)

Mouse Monoclonal
Species Human, Mouse
Applications WB, Flow, ICC/IF

     2 Reviews

27 Publications
WT1 Antibody (6F-H2) - BSA Free
NB110-60011
Western Blot: WT1 Antibody (6F-H2) [NB110-60011] - Hypomethylation of the Intron 1 CpG island results in WT1 expression. RNA was isolated from K562 and U937 cells growing under atmospheric conditions (Normox) and from U937 cells growing in 1% O2 (Hypox) and was analyzed by RT-PCR using primers that span exon 5 of the WT1 mRNA. Ribosomal RNA 36B4 is used as a positive control, and molecular weight markers (MW) are shown. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0119837), licensed under a CC-BY license.Immunohistochemistry-Paraffin: WT1 Antibody (6F-H2) [NB110-60011] - Staining of human kidney glomerulus tissue. Heat mediated antigen retrieval was performed by heating in citrate buffer (pH 6) at 95C for 20 minutes. Image from verified customer review.

Mouse Monoclonal
Species Human
Applications WB, Simple Western, ICC/IF

     3 Reviews

16 Publications
FOG2 Antibody
NBP1-32895
Western Blot: FOG2 Antibody [NBP1-32895] - A. 50 ug rat brain lysate/extract 5 % SDS-PAGE FOG2/ZFPM2 antibody dilution: 1:1000Western Blot: FOG2 Antibody [NBP1-32895] - Sample (50 ug of whole cell lysate) A: Mouse brain 5% SDS PAGE, antibody diluted at 1:3000.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB

     1 Review

1 Publication
RAR alpha/NR1B1 Antibody (763)
NB200-322
Western Blot: Retinoic Acid Receptor alpha Antibody (763) [NB200-322] - Analysis of hippocampal lysate showing specific immunolabeling of the ~48k RAR-a protein.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

     1 Review

1 Publication
Recombinant Human RAB40B GST (N-Term) Protein
H00010966-P01
12.5% SDS-PAGE Stained with Coomassie Blue.


Species Human
Applications WB, ELISA, PA

RNF70 Antibody
NBP1-87399
Western Blot: RNF70 Antibody [NBP1-87399] - Analysis in human cell line RT-4.Immunocytochemistry/Immunofluorescence: RNF70 Antibody [NBP1-87399] - Staining of human cell line U-2 OS shows localization to nucleus and nucleoli.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

HLX1 Antibody
NBP1-89719
Immunocytochemistry/Immunofluorescence: HLX1 Antibody [NBP1-89719] - Staining of human cell line HEK 293 shows positivity in nucleus and cytoplasm. Antibody staining is shown in green.Immunohistochemistry-Paraffin: HLX1 Antibody [NBP1-89719] - Staining of human tonsil shows strong nuclear positivity in reaction center cells.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

3 Publications
ACCN1 Antibody
NBP2-14321
Western Blot: ACCN1 Antibody [NBP2-14321] - Analysis in control (vector only transfected HEK293T lysate) and ASIC2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).Immunohistochemistry-Paraffin: ACCN1 Antibody [NBP2-14321] - Staining of human cerebral cortex shows high expression.

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

ZCCHV Antibody
NBP2-20940
Western Blot: ZCCHV Antibody [NBP2-20940] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZCCHV diluted at 1:10000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Immunocytochemistry/Immunofluorescence: ZCCHV Antibody [NBP2-20940] - ZCCHV antibody detects ZCCHV protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ZCCHV stained by ZCCHV antibody diluted at 1:1000. Blue: Fluoroshield with DAPI. Scale bar= 10 um.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

GATA-4 Antibody - BSA Free
NBP2-24585
Western Blot: GATA-4 Antibody [NBP2-24585] - Analysis of GATA-4 in human kidney lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.Immunohistochemistry-Paraffin: GATA-4 Antibody [NBP2-24585] - Analysis of a FFPE tissue section of human kidney using 1:200 dilution of GATA-4 antibody (NBP2-24585). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, IHC, IHC-P

     1 Review

2 Publications
BNC1 Antibody
NBP2-24721
Western Blot: BNC1 Antibody [NBP2-24721] - Analysis of BNC1 in human spleen lysate in the 1) absence and 2) presence of immunizing peptide, and 3) rat lysate using NBP2-24721 at 7 ug/ml and 4 ug/ml, respectively.

Rabbit Polyclonal
Species Human, Primate
Applications WB, KD

1 Publication
Ephrin-B1 Antibody [Unconjugated]
AF473
Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Ephrin-B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF473) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=Ephrin-B1 was detected in immersion fixed frozen sections of mouse kidney using Goat Anti-Mouse Ephrin-B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF473) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

Goat Polyclonal
Species Mouse
Applications WB, Simple Western, IHC

32 Publications
SOX7 Antibody [Unconjugated]
AF2766

Goat Polyclonal
Species Human
Applications WB, IHC

17 Publications
HGFR/c-MET Antibody [Unconjugated]
AF276
HGF R/c-MET was detected in immersion fixed HT-29 human colon adenocarcinoma cell line (positive control, left panel) and U937 human histiocytic lymphoma cell line (negative control, right panel) using Goat Anti-Human HGF R/c-MET Antigen Affinity-purified Polyclonal Antibody (Catalog # AF276) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; <a class=HGF R/c-MET was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human HGF R/c-MET Antigen Affinity-purified Polyclonal Antibody (Catalog # AF276) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (<a class=

Goat Polyclonal
Species Human
Applications WB, Simple Western, Flow

     1 Review

54 Publications
COUP-TF II/NR2F2 Antibody (H7147) [Unconjugated]
PP-H7147-00
Reduction of COUP-TFII or nucleolin decreases RAR beta 2 transcription in MCF-7 cells.MCF-7 (A) and T47D (B) cells were transfected with control siRNA or an siRNA targeting nucleolin for 48 h. T47D cells were treated with EtOH or 1 µM atRA for 24 h. Q-PCR for nucleolin (NCL) and RARB2. Values are the average of triplicates. C, Western blot showing COUP-TFII and RAR beta 2 expression after transfection with siCOUP-TFII. Values are relative to beta -actin. MCF-7 were transfected with siControl or siCOUP-TFII for 48 h and treated with 1 µM atRA for 6 h. Q-PCR was also performed for RRIG1. P<0.001 * versus control or ** versus atRA. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0038278), licensed under a CC-BY license. Not internally tested by R&D Systems.Loss of miR-101 promotes cancer metastasis through de-repression of COUP-TFII expression.(a) LNCaP and PC3 cells were individually treated with 50 nM of miR-101 and miR-27a mimic for 48 h and then invasion assays were performed for an additional 16 h (n=3). *P<0.05 (two-sided Student's t-test) compared with the control group. (b) Representative western blot showed the levels of COUP-TFII, E-cadherin, vimentin and beta -actin in PC3 cells stably knocked down by COUP-TFII. (c) LNCaP and PC3 cells stably knocked-down of COUP-TFII were used to perform cell invasion assay. Invaded cells were stained and counted (lower panel) (n=3). Representative western blot showed the knockdown efficiency of COUP-TFII (upper panel). *: P<0.05 (two-sided Student's t-test) compared with control group. (d) PC3 cells carrying an inducible miR-101 in the absence and presence of doxycycline, and re-expression of COUP-TFII were used to perform invasion assays (n=3). Representative western blot showed the levels of COUP-TFII and beta -actin (left). Invaded cells were counted and results are shown in the right panel. *: P<0.05 (two-sided Student's t-test) (e) 22RV-1 cells were treated with miR-101 inhibitor (antisense RNA) in conjunction with knockdown of COUP-TFII and used for invasion assays (n=3). Representative western blot show the levels of COUP-TFII and beta -actin (left). Invaded cells were counted and result is shown in the right panel. *: P<0.05 (two-sided Student's t-test). (f) LNCaP cells containing a construct with inducible expression of COUP-TFII shRNA and constructs expressing with anti-miR-101 and anti-mir-27a were orthotopically injected into NOD-SCID mouse prostate. In addition these cells also contain a luciferase reporter to detect cancer cells. After the tumour size was bigger than 50 mm3, drinking water with or without doxycycline (1 mg ml−1) was given to the mice for 6 weeks to induce the COUP-TFII shRNA to repress COUP-TFII expression. Representative bioluminescence results show the status of tumour growth in different groups. (g) Quantification result of luminance from IVIS (control: n=5; anti-miR-101/27a(−): n=6; anti-miR-101/27a(+): n=7. *: P<0.05 (two-sided Student's t-test). (h) Immunohistochemical stain showed the metastatic tumour cells located in the mouse lymph node are positive for AR. Scale bar: 100 μM (low power); and 200 μM (high power). SCID, severe combined immunodeficient. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms11418), licensed under a CC-BY license. Not internally tested by R&D Systems.

Mouse Monoclonal
Species Human
Applications WB, IHC, IP

     3 Reviews

46 Publications
COUP-TF I/NR2F1 Antibody (H8132) [Unconjugated]
PP-H8132-00

Mouse Monoclonal
Species Human
Applications WB, IHC, IP

13 Publications
FGF-10 [Unconjugated]
345-FG
Recombinant Human FGF-10 (Catalog # 345-FG) stimulates cell proliferation of the 4MBr‑5 rhesus monkey epithelial cell line. The ED<SUB>50</SUB> for this effect is 20‑100 ng/mL. 1 μg/lane of Recombinant Human FGF-10 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 21 kDa.


Species Human
Applications BA

103 Publications
MYF-5 Antibody (OTI2G5)
NBP2-44245
Western Blot: MYF-5 Antibody (OTI2G5) [NBP2-44245] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MYF5 , (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MYF5Immunohistochemistry-Paraffin: MYF-5 Antibody (OTI2G5) [NBP2-44245] - Carcinoma of Human prostate tissue using anti-MYF5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, IHC, IHC-P

beta-Galactosidase-1/GLB1 Antibody (OTI1C9)
NBP2-45731
Western Blot: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) [NBP2-45731] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GLB1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).Immunohistochemistry: beta-Galactosidase-1/GLB1 Antibody (1C9) [NBP2-45731] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Mouse Monoclonal
Species Human, Canine, Monkey
Applications WB, IHC, IHC-P

6 Publications