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Cardiac Conduction System Development Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Cardiac Conduction System Development Pathway and Heart Diseases, Congenital Heart Defects, Congenital Heart Disease, Cardiac Arrhythmia, Haploinsufficiency. The study of the Cardiac Conduction System Development Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Cardiac Conduction System Development Pathway has been researched in relation to System Development, Cardiac Conduction, Developmental Process. The Cardiac Conduction System Development Pathway complements our catalog of research reagents including antibodies and ELISA kits against HF-1B, CARDIAC TROPONIN I, BDNF, NKX2-5, DCX.

Top Research Reagents

We have 853 products for the study of the Cardiac Conduction System Development Pathway that can be applied to Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

MAP4K6 Antibody

NBP1-22990

Western Blot: MAP4K6 Antibody [NBP1-22990] - Whole cell lysate from HeLa and mouse NIH3T3 cells at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, IP

5 Publications

MyoD Antibody (5.8A) - BSA Free

NB100-56511

Immunocytochemistry/Immunofluorescence: MyoD Antibody (5.8A) [NB100-56511] - Human myoblasts were stained with MyoD1 antibody, diluted 1:200. ICC/IF image submitted by a verified customer review.

Western Blot: MyoD Antibody (5.8A) [NB100-56511] - Insuline-like growth factor (IGF1) antagonized the effects of miR-106a-5p on myogenic differentiation in C2C12. All data were collected from C2C12 myotubes 5 d post differentiation. Western-blot analysis of myogenic regulatory factors (MyoD, MyoG, MyHC) in cells; (H) The statistical results of Figure 3G. Data were presented as mean +/- SEM. n = 3 per group. * p < 0.05, ** p < 0.01. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/2073-4425/9/7/333/htm) licensed under a CC-BY license. anti-MyoG (catalog# NB100-56510)

Mouse Monoclonal
Species Human, Mouse
Applications WB, Flow, ICC/IF

2 Reviews

27 Publications

KCNE1 Antibody (5B12)

H00003753-M01

Western Blot: KCNE1 Antibody (5B12) [H00003753-M01] - Analysis of KCNE1 over-expressed 293 cell line, cotransfected with KCNE1 Validated Chimera RNAi ( Cat # H00003753-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with KCNE1 monoclonal antibody (M01), clone 5B12 (Cat # H00003753-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: KCNE1 Antibody (5B12) [H00003753-M01] - analysis of KCNE1 expression in transfected 293T cell line by KCNE1 monoclonal antibody (M01), clone 5B12. Lane 1: KCNE1 transfected lysate (14.7 KDa). Lane 2: Non-transfected lysate.

Mouse Monoclonal
Species Human, Rat, Canine
Applications WB, ELISA, ICC/IF

4 Publications

Connexin 40/GJA5 Antibody

NBP3-41541

Western Blot: Connexin 40/GJA5 Antibody [NBP3-41541] - Sample: Recombinant protein.

Immunohistochemistry-Paraffin: Connexin 40/GJA5 Antibody [NBP3-41541] - DAB staining on IHC-P; Samples: Rat Brain Tissue

Rabbit Polyclonal
Species Rat
Applications WB, ICC/IF, IHC

Doublecortin Antibody

NBP1-72042

Western Blot: Doublecortin Antibody [NBP1-72042] - staining of Mouse fetal Brain lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: Doublecortin Antibody [NBP1-72042] - Immunofluorescence analysis of paraformaldehyde fixed KNRK cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Goat Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

5 Publications

CCS/SOD4 Antibody (1E2)

H00009973-M03

Western Blot: CCS/SOD4 Antibody (1E2) [H00009973-M03] - Analysis of CCS expression in transfected 293T cell line by CCS monoclonal antibody (M03), clone 1E2. Lane 1: CCS transfected lysate (Predicted MW: 29 KDa). Lane 2: Non-transfected lysate.

Immunohistochemistry-Paraffin: CCS/SOD4 Antibody (1E2) [H00009973-M03] - Analysis of monoclonal antibody to CCS on formalin-fixed paraffin-embedded human kidney. Antibody concentration 1.5 ug/ml

Mouse Monoclonal
Species Human
Applications WB, ELISA, ICC/IF

1 Publication

TBX5 Antibody

NBP1-83237

Immunohistochemistry-Paraffin: TBX5 Antibody [NBP1-83237] - Staining of human placenta shows moderate nuclear positivity in Hofbauer cells, as well as weaker positivity in trophoblastic cells.

Immunohistochemistry-Paraffin: TBX5 Antibody [NBP1-83237] - Staining of human heart muscle shows strong nuclear positivity in cardiomyocytes.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ICC/IF, IHC

4 Publications

MSX2 Antibody

NBP1-85445

Immunohistochemistry-Paraffin: MSX2 Antibody [NBP1-85445] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.

Immunohistochemistry-Paraffin: MSX2 Antibody [NBP1-85445] - Staining of human urinary bladder shows weak nuclear positivity in urothelial cells.

Rabbit Polyclonal
Species Human
Applications IHC, IHC-P

4 Publications

SS18L1 Antibody

NBP2-20486

Western Blot: SS18L1 Antibody [NBP2-20486] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: SS18L1 Antibody [NBP2-20486] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using antibody at 1:500 dilution.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ICC/IF, IHC

GATA-4 Antibody - BSA Free

NBP2-24585

Western Blot: GATA-4 Antibody [NBP2-24585] - Analysis of GATA-4 in human kidney lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Immunohistochemistry-Paraffin: GATA-4 Antibody [NBP2-24585] - Analysis of a FFPE tissue section of human kidney using 1:200 dilution of GATA-4 antibody (NBP2-24585). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, IHC, IHC-P

1 Review

2 Publications

ID2 Antibody

NBP2-27194

Western Blot: ID2 Antibody [NBP2-27194] - Analysis using ID2 antibody. Lysate from human brain in the 1) absence and 2) presence of immunizing peptide, 3) mouse and 4) rat brain probed with ID2 antibody at 2 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Immunocytochemistry/Immunofluorescence: ID2 Antibody [NBP2-27194] - IF Confocal analysis of A549 cells using ID2 antibody (NBP2-27194, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue). Image from verified customer review.

Rabbit Polyclonal
Species Human, Rat, Bovine
Applications WB, ICC/IF

1 Review

2 Publications

SP4 Antibody

NBP2-31983

Immunocytochemistry/Immunofluorescence: SP4 Antibody [NBP2-31983] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.

Immunohistochemistry-Paraffin: SP4 Antibody [NBP2-31983] - Staining of human lymph node shows distinct nuclear positivity in non-germinal center cells.

Rabbit Polyclonal
Species Human
Applications ICC/IF, IHC, IHC-P

TrkC Antibody [Unconjugated]

AF1404

Western blot shows lysates of mouse brain (cerebellum) tissue, mouse brain (cortex) tissue, and rat brain tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse/Rat TrkC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1404) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

Simple Western lane view shows lysates of mouse brain tissue, loaded at 0.2&nbsp;mg/mL. Specific bands were detected for TrkC at approximately 111 and 159&nbsp;kDa (as indicated) using 25&nbsp;µg/mL of Goat Anti-Mouse/Rat TrkC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1404) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog&nbsp;# <a class=

Goat Polyclonal
Species Mouse, Rat
Applications WB, Simple Western, IHC

1 Review

66 Publications

GATA-6 Antibody [Unconjugated]

AF1700

Western blot shows cytoplasmic and nuclear extracts from PC-3 human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human GATA-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1700) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

GATA-6 was detected in immersion fixed KATO-III human gastric carcinoma cell line using 10 µg/mL Goat Anti-Human GATA-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1700) for 3 hours at room temperature. Cells were stained with the NorthernLights&#8482; 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; <a class=

Goat Polyclonal
Species Human
Applications WB, Simple Western, IHC

6 Reviews

134 Publications

NKX2.5 Antibody [Unconjugated]

AF2444

EOMES programs hESCs into functional CMs at high efficiency. a Illustration of growth factor-mediated and optimized EOMES induction-based cardiac differentiation protocols. Bottom right: Immunoblot validating doxycycline-dependent EOMES expression in a transgenic EOMESKO/E.TET-ON hESC line. b Typical yields of hESC-CMs (left, flow cytometry) and NKX2.5 expression (right, immunoblot) obtained with the two protocols (day 10). c Immunostainings 21 days after the initiation of EOMES induction. Weak perinuclear ANP staining is typical in overall MLC2v-positive hESC-CMs. Scale bars: 25 (top) and 50 µm (bottom). d Acceleration and slowdown of spontaneous beat rates in pCMs following exposure to 10 µM isoprenaline and 10 µM propranolol, respectively, on multielectrode arrays. e Microarray-based time course analysis comparing the indicated protocols and cell lines. RESCUE cells carry an inducible EOMES transgene on EOMESKO HuES6 background. Underlying data are from Supplementary Data 2 Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29382828), licensed under a CC-BY license. Not internally tested by R&D Systems.

Contextual requirements of EOMES-mediated CM programming. a DOX dose dependency of the EOMES TET-ON protocol using the WTE.TET-ON hESC line. Top panel: Immunostains at 1.5 wk. Scale bar: 100 µm. Bottom: Flow cytometry analysis. Numbers indicate average percentages of cTnT-positive CMs from 3–6 experiments per condition. b CM programming by EOMES necessitates suppression of autocrine WNT signaling from the third day of transgene induction (qPCR data, n = 2). c DOX dose-dependent CM programming using an independent WTE.TET-ON hiPSC line. The data shows immunostaining (scale bar: 100 µm), RT-qPCR (n = 4), and FACS analysis (n = 3) performed at 1 wk of differentiation. The asymmetrical shape of the DOX titration data with this line is in part due to the incomplete repression of SOX2 at 0.05 µg/ml, which caused overgrowth of the cultures with neural precursors (also see Supplementary Fig. 3f). Error bars: s.e.m. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29382828), licensed under a CC-BY license. Not internally tested by R&D Systems.

Goat Polyclonal
Species Human
Applications WB, IHC

2 Reviews

13 Publications

ID1 Antibody [Unconjugated]

AF4377

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and MCF-7 human breast cancer cell line. Gels were loaded with 20 µg of cytoplasmic (Cyto) and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse ID1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4377) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

ID1 was detected in immersion fixed BG01V human embryonic stem cells, undifferentiated (lower panel) and differentiated into neural progenitor cells (upper panel), using Goat Anti-Human/Mouse ID1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4377) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights&#8482; 557-conjugated Anti-Goat IgG Secondary Antibody (red; <a class=

Goat Polyclonal
Species Human, Mouse
Applications WB, Simple Western, ICC

4 Publications

TBX3 Antibody [Unconjugated]

AF4509

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TBX3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4509) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

Western blot shows lysates of LNCaP human prostate cancer cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2&nbsp;µg/mL of Goat Anti-Human TBX3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4509) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

Goat Polyclonal
Species Human
Applications WB, ICC

1 Review

2 Publications

BDNF [Unconjugated]

248-BDB

Equivalent bioactivity of CHO-derived (<a class=NoLineLink href=

Species Human, Mouse, Rat
Applications Bind, BA

238 Publications

Related Genes

The Cardiac Conduction System Development Pathway has been researched against:

Related Pathways

The Cardiac Conduction System Development Pathway has been linked to:

Related Diseases

The Cardiac Conduction System Development Pathway has been studied in relation to diseases such as:

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