![Western Blot: Recombinant Human Lgi1 Protein [H00009211-P01] - The human recombinant LGI1 protein is recognized by a commercial antibody against LGI1. Lane 2: 0.4 ug; Lane 3: 0.2 ug). Lane 4: no protein. Image from verified customer review.](http://images.novusbio.com/fullsize/Recombinant-Human-Lgi1-Protein-Western-Blot-H00009211-P01-img0002.jpg "Western Blot: Recombinant Human Lgi1 Protein [H00009211-P01] - The human recombinant LGI1 protein is recognized by a commercial antibody against LGI1. Lane 2: 0.4 ug; Lane 3: 0.2 ug). Lane 4: no protein. Image from verified customer review.")
| Description | A recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-557 of Human LGI1 Source: Wheat Germ (in vitro) Amino Acid Sequence: MESERSKRMGNACIPLKRIAYFLCLLSALLLTEGKKPAKPKCPAVCTCTKDNALCENARSIPRTVPPDVISLSFVRSGFTEISEGSFLFTPSLQLLLFTSNSFDVISDDAFIGLPHLEYLFIENNNIKSISRHTFRGLKSLIHLSLANNNLQTLPKDIFKGLDSLTNVDLRGNSFNCDCKLKWLVEWLGHTNATVEDIYCEGPPEYKKRKINSLSSKDFDCIITEFAKSQDLPYQSLSIDTFSYLNDEYVVIAQPFTGKCIFLEWDHVEKTFRNYDNITGTSTVVCKPIVIETQLYVIVAQLFGGSHIYKRDSFANKFIKIQDIEILKIRKPNDIETFKIENNWYFVVADSSKAGFTTIYKWNGNGFYSHQSLHAWYRDTDVEYLEIVRTPQTLRTPHLILSSSSQRPVIYQWNKATQLFTNQTDIPNMEDVYAVKHFSVKGDVYICLTRFIGDSKVMKWGGSSFQDIQRMPSRGSMVFQPLQINNYQYAILGSDYSFTQVYNWDAEKAKFVKFQELNVQAPRSFTHVSINKRNFLFASSFKGNTQIYKHVIVDLSA |
| Preparation Method |
in vitro wheat germ expression system |
| Details of Functionality | This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated. |
| Source | Wheat germ |
| Protein/Peptide Type | Recombinant Protein |
| Gene | LGI1 |
| Purity | >80% by SDS-PAGE and Coomassie blue staining |
| Dilutions |
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| Theoretical MW | 86.68 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Reviewed Applications |
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| Storage | Store at -80C. Avoid freeze-thaw cycles. |
| Buffer | 50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer. |
| Preservative | No Preservative |
| Purity | >80% by SDS-PAGE and Coomassie blue staining |
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| Publication using H00009211-P01 | Applications | Species |
|---|---|---|
| Petit-Pedrol M, Sell J, Planaguma J et al. LGI1 antibodies alter Kv1.1 and AMPA receptors changing synaptic excitability, plasticity and memory. Brain. 2018-11-01 [PMID: 30346486] |
| Images | Ratings | Applications | Species | Date | Details | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
Enlarge |
reviewed by:
Mar Petit-Pedrol |
SDS-PAGE Control | 10/23/2018 |
Summary
Comments
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| Gene Symbol | LGI1 |
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