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![Immunocytochemistry/Immunofluorescence: Thioredoxin Antibody [NBP2-52983] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Thioredoxin at a 1:100 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-52983-img0001.jpg "Immunocytochemistry/Immunofluorescence: Thioredoxin Antibody [NBP2-52983] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Thioredoxin at a 1:100 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Immunocytochemistry/Immunofluorescence: Thioredoxin Antibody [NBP2-52983] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Thioredoxin at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-52983-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Thioredoxin Antibody [NBP2-52983] - PC12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Thioredoxin at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-52983-img0006.jpg)
![Western Blot: Thioredoxin Antibody [NBP2-52983] - Total protein from human HeLa cells, mouse 3T3 cells and rat PC12 cells was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um pore size PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Thioredoxin in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Western-Blot-NBP2-52983-img0003.jpg)
![Immunohistochemistry-Paraffin: Thioredoxin Antibody [NBP2-52983] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse intestine using rabbit polyclonal antibody against Thioredoxin (1:300 dilution). The signal was developed with HRP-secondary -DAB method and the sections were counter-stained using hematoxylin. The staining was found primarily in the epithelial cells of the intestinal lumen with weak to negligible signal in the basal or muscular layers. Isolated epithelial cells depicted nuclear positivity also.](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Immunohistochemistry-Paraffin-NBP2-52983-img0002.jpg)
![Flow Cytometry: Thioredoxin-1 Antibody [NBP2-52983] - An intracellular stain was performed on HeLa cells with Thioredoxin-1 Antibody NBP2-52983AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Thioredoxin-1-Antibody-Flow-Cytometry-NBP2-52983-img0007.jpg)
![Flow Cytometry: Thioredoxin-1 Antibody [NBP2-52983] - Thioredoxin Antibody [NBP2-52983] - An intracellular stain was performed on U-87 MG cells with Thioredoxin Antibody NBP2-52983 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Flow-Cytometry-NBP2-52983-img0004.jpg)
![Western Blot: Thioredoxin Antibody [NBP2-48873] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MGLane 4: Human plasmaLane 5: Human Liver tissueLane 6: Human Tonsil tissue](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Western-Blot-NBP2-48873-img0003.jpg "Western Blot: Thioredoxin Antibody [NBP2-48873] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MGLane 4: Human plasmaLane 5: Human Liver tissueLane 6: Human Tonsil tissue")
![Immunocytochemistry/Immunofluorescence: Thioredoxin Antibody [NBP2-48873] - Staining of human cell line U-251 MG shows localization to nucleus and cytosol. Antibody staining is shown in greeen.](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48873-img0009.jpg)
![Immunohistochemistry-Paraffin: Thioredoxin Antibody [NBP2-48873] - Staining of human kidney.](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Immunohistochemistry-Paraffin-NBP2-48873-img0012.jpg)
![Immunohistochemistry-Paraffin: Thioredoxin Antibody [NBP2-48873] - Staining of human esophagus shows high expression.](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Immunohistochemistry-Paraffin-NBP2-48873-img0006.jpg)
![Immunohistochemistry-Paraffin: Thioredoxin Antibody [NBP2-48873] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Immunohistochemistry-Paraffin-NBP2-48873-img0007.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Thioredoxin Antibody [NBP2-48873] - Staining in human esophagus and skeletal muscle tissues using anti-TXN antibody. Corresponding TXN RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Immunohistochemistry-Paraffin-NBP2-48873-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Thioredoxin Antibody [NBP2-48873] - Staining of human colon, esophagus, kidney and skeletal muscle using Anti-TXN antibody NBP2-48873 (A) shows similar protein distribution across tissues to independent antibody NBP2-49191 (B).](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Immunohistochemistry-Paraffin-NBP2-48873-img0010.jpg)
![Immunohistochemistry-Paraffin: Thioredoxin Antibody [NBP2-48873] - Staining of human colon.](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Immunohistochemistry-Paraffin-NBP2-48873-img0011.jpg)
![Western Blot: Thioredoxin Antibody (2A7) [H00007295-M01] - TXN monoclonal antibody (M01), clone 2A7 Analysis of TXN expression in HeLa.](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-2A7-Western-Blot-H00007295-M01-img0008.jpg "Western Blot: Thioredoxin Antibody (2A7) [H00007295-M01] - TXN monoclonal antibody (M01), clone 2A7 Analysis of TXN expression in HeLa.")
![Immunocytochemistry/Immunofluorescence: Thioredoxin Antibody (2A7) [H00007295-M01] - Analysis of monoclonal antibody to TXN on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-2A7-Immunocytochemistry-Immunofluorescence-H00007295-M01-img0005.jpg)
![Western Blot: Thioredoxin Antibody (2A7) [H00007295-M01] - Analysis of TXN expression in transfected 293T cell line by TXN monoclonal antibody (M01), clone 2A7.Lane 1: TXN transfected lysate(11.7 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-2A7-Western-Blot-H00007295-M01-img0009.jpg)
![Immunoprecipitation: Thioredoxin Antibody (2A7) [H00007295-M01] - Analysis of TXN transfected lysate using anti-TXN monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with TXN MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-2A7-Immunoprecipitation-H00007295-M01-img0006.jpg)
![ELISA: Thioredoxin Antibody (2A7) [H00007295-M01] - Detection limit for recombinant GST tagged TXN is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-2A7-ELISA-H00007295-M01-img0010.jpg)
![Immunohistochemistry-Paraffin: Thioredoxin-1 Antibody [NBP2-49191] - Thioredoxin Antibody [NBP2-49191] - Staining in human esophagus and skeletal muscle tissues using anti-TXN antibody. Corresponding TXN RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Immunohistochemistry-Paraffin-NBP2-49191-img0004.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Thioredoxin-1 Antibody [NBP2-49191] - Thioredoxin Antibody [NBP2-49191] - Staining in human esophagus and skeletal muscle tissues using anti-TXN antibody. Corresponding TXN RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Thioredoxin-1 Antibody [NBP2-49191] - Thioredoxin Antibody [NBP2-49191] - Staining of human colon, esophagus, kidney and skeletal muscle using Anti-TXN antibody NBP2-49191 (A) shows similar protein distribution across tissues to independent antibody NBP2-48873 (B).](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Immunohistochemistry-Paraffin-NBP2-49191-img0005.jpg)
![Immunohistochemistry-Paraffin: Thioredoxin Antibody [NBP2-49191] - Staining of human kidney.](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Immunohistochemistry-Paraffin-NBP2-49191-img0008.jpg)
![Immunohistochemistry-Paraffin: Thioredoxin Antibody [NBP2-49191] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Immunohistochemistry-Paraffin-NBP2-49191-img0003.jpg)
![Immunohistochemistry-Paraffin: Thioredoxin Antibody [NBP2-49191] - Staining of human esophagus shows high expression.](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Immunohistochemistry-Paraffin-NBP2-49191-img0006.jpg)
![Immunohistochemistry-Paraffin: Thioredoxin Antibody [NBP2-49191] - Staining of human colon.](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Immunohistochemistry-Paraffin-NBP2-49191-img0007.jpg)
![Western Blot: Thioredoxin Antibody [NBP1-31090] - A: H1299 B: HeLa S3 12% SDS PAGE diluted at 1:3000](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Western-Blot-NBP1-31090-img0001.jpg "Western Blot: Thioredoxin Antibody [NBP1-31090] - A: H1299 B: HeLa S3 12% SDS PAGE diluted at 1:3000")
![Immunocytochemistry/Immunofluorescence: Thioredoxin Antibody [NBP1-31090] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Trx protein stained by Trx antibody [N3C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31090-img0004.jpg)
![Western Blot: Thioredoxin-1 Antibody (116) [NBP2-89458] - Lane A: Hela Whole Cell Lysate Lane B: THP-1 Whole Cell LysateLane C: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:12 kDa Observed band size:14 kDa](http://images.novusbio.com/fullsize/Thioredoxin-1-Antibody-116-Western-Blot-NBP2-89458-img0001.jpg "Western Blot: Thioredoxin-1 Antibody (116) [NBP2-89458] - Lane A: Hela Whole Cell Lysate Lane B: THP-1 Whole Cell LysateLane C: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:12 kDa Observed band size:14 kDa")
![Immunoprecipitation: Thioredoxin-1 Antibody (116) [NBP2-89458] - Lane A:0.5 mg Hela Whole Cell Lysate Lane B:0.5 mg 293T Whole Cell Lysate2 uL anti-TXN rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody: Anti-TXN rabbit monoclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 13 kDa](http://images.novusbio.com/fullsize/Thioredoxin-1-Antibody-116-Immunoprecipitation-NBP2-89458-img0002.jpg)
![Western Blot: Thioredoxin-1 Antibody (S07-4H5) [NBP3-19933] - Western blot detection of Thioredoxin-1 in Jurkat, Hela cell lysates using NBP3-19933 (1:1000 diluted). Predicted band size: 12kDa. Observed band size: 12kDa.](http://images.novusbio.com/fullsize/Thioredoxin-1-Antibody-S07-4H5-Western-Blot-NBP3-19933-img0002.jpg "Western Blot: Thioredoxin-1 Antibody (S07-4H5) [NBP3-19933] - Western blot detection of Thioredoxin-1 in Jurkat, Hela cell lysates using NBP3-19933 (1:1000 diluted). Predicted band size: 12kDa. Observed band size: 12kDa.")
![Immunohistochemistry-Paraffin: Thioredoxin-1 Antibody (S07-4H5) [NBP3-19933] - Immunohistochemistry of Thioredoxin-1 in paraffin-embedded Human tonsil using NBP3-19933 at dilution 1/100](http://images.novusbio.com/fullsize/Thioredoxin-1-Antibody-S07-4H5-Immunohistochemistry-Paraffin-NBP3-19933-img0001.jpg)
![Western Blot: Thioredoxin-1 Antibody (3G7) [NBP3-26265] - Positive Western Blot detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, Rat brain tissue. All lanes: Thioredoxin-1 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 10, 12 kDa Observed band size: 12 kDa](http://images.novusbio.com/fullsize/nbp3-26265_rabbit-thioredoxin-1-mab-3g7-282202411442643.jpg "Western Blot: Thioredoxin-1 Antibody (3G7) [NBP3-26265] - Positive Western Blot detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, Rat brain tissue. All lanes: Thioredoxin-1 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 10, 12 kDa Observed band size: 12 kDa")
![SDS-Page: Recombinant E. coli Thioredoxin Protein [NBP1-44476] - 115% SDS-Page (3ug)](http://images.novusbio.com/fullsize/Recombinant-E.-coli-Thioredoxin-Protein-SDS-Page-NBP1-44476-img0001.jpg "SDS-Page: Recombinant E. coli Thioredoxin Protein [NBP1-44476] - 115% SDS-Page (3ug)")