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![Immunohistochemistry-Paraffin: TRAF-4 Antibody [NB100-56177] - Formalin-fixed, paraffin embedded, normal human mammary epithelial tissue using th antibody. A-C: Strong TRAF4 staining is seen in the luminal epithelial cells of the interlobular terminal ducts (acini). However, there is absence of TRAF4 immunostaining in the myoepithelial cells along the basement membrane. B and C: higher magnifications of A. Data courtesy of Krajewska et al.](http://images.novusbio.com/fullsize/TRAF-4-Antibody-Immunohistochemistry-Paraffin-NB100-56177-img0008.jpg "Immunohistochemistry-Paraffin: TRAF-4 Antibody [NB100-56177] - Formalin-fixed, paraffin embedded, normal human mammary epithelial tissue using th antibody. A-C: Strong TRAF4 staining is seen in the luminal epithelial cells of the interlobular terminal ducts (acini). However, there is absence of TRAF4 immunostaining in the myoepithelial cells along the basement membrane. B and C: higher magnifications of A. Data courtesy of Krajewska et al.")
![Immunoprecipitation: TRAF-4 Antibody [NB100-56177] - The NB100-56177 TRAF4 antibody specifically immunoprecipitates TRAF4. Top: TRAFs 2,3,4,5, and 6 were in vitro translated in the presence of 35S-methionine, and immunoprecipiated using the TRAF4 antibody. The TRAF4 antibody immunoprecipitated only TRAF4 and did not recognize TRAFs 2, 3, 5, or 6. A reticulocyte primed with an empty pcDNA plasmid was used as a negative control. Bottom: SDS-PAGE visualization of the in vitro translated TRAFs. Data courtesy of Krajewska et al.](http://images.novusbio.com/fullsize/TRAF-4-Antibody-Immunoprecipitation-NB100-56177-img0009.jpg)
![Western Blot: TRAF-4 Antibody [NBP2-33647] - Analysis in human cell lines HeLa and PC-3. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.](http://images.novusbio.com/fullsize/TRAF-4-Antibody-Western-Blot-NBP2-33647-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: TRAF-4 Antibody [NBP2-33647] - Analysis in human cell lines HeLa and PC-3. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: TRAF-4 Antibody [NBP2-33647] - Staining in human gallbladder and skeletal muscle tissues using anti-TRAF4 antibody. Corresponding TRAF4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/TRAF-4-Antibody-Immunohistochemistry-Paraffin-NBP2-33647-img0007.jpg)
![Western Blot: TRAF-4 Antibody [NBP2-33647] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/TRAF-4-Antibody-Western-Blot-NBP2-33647-img0004.jpg)
![Immunohistochemistry-Paraffin: TRAF-4 Antibody [NBP2-33647] - Staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.](http://images.novusbio.com/fullsize/TRAF-4-Antibody-Immunohistochemistry-Paraffin-NBP2-33647-img0001.jpg)
![Immunohistochemistry-Paraffin: TRAF-4 Antibody [NBP2-33647] - Staining of liver.](http://images.novusbio.com/fullsize/TRAF-4-Antibody-Immunohistochemistry-Paraffin-NBP2-33647-img0002.jpg)
![Immunohistochemistry-Paraffin: TRAF-4 Antibody [NBP2-33647] - Staining of breast cancer.](http://images.novusbio.com/fullsize/TRAF-4-Antibody-Immunohistochemistry-Paraffin-NBP2-33647-img0003.jpg)
![Immunohistochemistry-Paraffin: TRAF-4 Antibody [NBP2-33647] - Staining of human gallbladder shows high expression.](http://images.novusbio.com/fullsize/TRAF-4-Antibody-Immunohistochemistry-Paraffin-NBP2-33647-img0005.jpg)
![Immunohistochemistry-Paraffin: TRAF-4 Antibody [NBP2-33647] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/TRAF-4-Antibody-Immunohistochemistry-Paraffin-NBP2-33647-img0006.jpg)
![Western Blot: TRAF-4 Antibody [NBP3-17200] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: RT4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: Tonsil](http://images.novusbio.com/fullsize/TRAF-4-Antibody-Western-Blot-NBP3-17200-img0002.jpg "Western Blot: TRAF-4 Antibody [NBP3-17200] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: RT4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: Tonsil")
![Immunocytochemistry/Immunofluorescence: TRAF-4 Antibody [NBP3-17200] - Staining of human cell line RT4 shows localization to nucleoplasm, nucleoli fibrillar center & cytosol.](http://images.novusbio.com/fullsize/TRAF-4-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-17200-img0001.jpg)
![Western Blot: TRAF-4 Antibody (SR1969) [NBP3-21648] - Western blot analysis using NBP3-21648 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-21648_rabbit-traf-4-mab-sr1969-572023153124.jpg "Western Blot: TRAF-4 Antibody (SR1969) [NBP3-21648] - Western blot analysis using NBP3-21648 in HeLa cell lysate.")
![Western Blot: TRAF4 Overexpression Lysate (Adult Normal) [NBL1-17244] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TRAF4.](http://images.novusbio.com/fullsize/TRAF4-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17244-img0002.jpg "Western Blot: TRAF4 Overexpression Lysate (Adult Normal) [NBL1-17244] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TRAF4.")