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![Immunohistochemistry-Paraffin: RTN3 Antibody [NBP1-88871] - Staining of human cerebral cortex, colon, liver and lymph node using Anti-RTN3 antibody (A) NBP1-88871 shows similar protein distribution across tissues to independent antibody NBP1-88870 (B).](http://images.novusbio.com/fullsize/RTN3-Antibody-Immunohistochemistry-Paraffin-NBP1-88871-img0018.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: RTN3 Antibody [NBP1-88871] - Staining of human cerebral cortex, colon, liver and lymph node using Anti-RTN3 antibody (A) NBP1-88871 shows similar protein distribution across tissues to independent antibody NBP1-88870 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: RTN3 Antibody [NBP1-88871] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/RTN3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88871-img0005.jpg)
![Immunohistochemistry-Paraffin: RTN3 Antibody [NBP1-88871] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/RTN3-Antibody-Immunohistochemistry-Paraffin-NBP1-88871-img0014.jpg)
![Immunohistochemistry-Paraffin: RTN3 Antibody [NBP1-88871] - Staining of human colon shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/RTN3-Antibody-Immunohistochemistry-Paraffin-NBP1-88871-img0015.jpg)
![Immunohistochemistry-Paraffin: RTN3 Antibody [NBP1-88871] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/RTN3-Antibody-Immunohistochemistry-Paraffin-NBP1-88871-img0016.jpg)
![Immunohistochemistry-Paraffin: RTN3 Antibody [NBP1-88871] - Staining of human lymph node shows no positivity in lymphoid cells as expected.](http://images.novusbio.com/fullsize/RTN3-Antibody-Immunohistochemistry-Paraffin-NBP1-88871-img0017.jpg)
![Immunohistochemistry-Paraffin: RTN3 Antibody [NBP1-88870] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/RTN3-Antibody-Immunohistochemistry-Paraffin-NBP1-88870-img0009.jpg "Immunohistochemistry-Paraffin: RTN3 Antibody [NBP1-88870] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.")
![Immunohistochemistry-Paraffin: RTN3 Antibody [NBP1-88870] - Staining of human colon shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/RTN3-Antibody-Immunohistochemistry-Paraffin-NBP1-88870-img0010.jpg)
![Immunohistochemistry-Paraffin: RTN3 Antibody [NBP1-88870] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/RTN3-Antibody-Immunohistochemistry-Paraffin-NBP1-88870-img0011.jpg)
![Immunohistochemistry-Paraffin: RTN3 Antibody [NBP1-88870] - Staining of human lymph node shows no positivity in lymphoid cells as expected.](http://images.novusbio.com/fullsize/RTN3-Antibody-Immunohistochemistry-Paraffin-NBP1-88870-img0012.jpg)
![Western Blot: RTN3 Antibody (PSH0-40) [NBP3-32921] - Western blot analysis of RTN3 on different lysates with Rabbit anti-RTN3 antibody (NBP3-32921) at 1/1,000 dilution. Lane 1: HeLa cell lysate (32 ug/Lane) Lane 2: HEK-293 cell lysate (33 ug/Lane) Lane 3: U87-MG cell lysate (30 ug/Lane) Lane 4: A549 cell lysate (26 ug/Lane) Lane 5: SH-SY5Y cell lysate (18 ug/Lane) Lane 6: PC-12 cell lysate (23 ug/Lane) Lane 7: L6 cell lysate (14 ug/Lane) Lane 8: Human cerebellum tissue lysate (40 ug/Lane) Lysates/proteins at 10 ug/Lane. Predicted band size: 112.6 kDa Observed band size: 100 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32921) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32921_rabbit-rtn3-mab-psh0-40-288202412461667.jpg "Western Blot: RTN3 Antibody (PSH0-40) [NBP3-32921] - Western blot analysis of RTN3 on different lysates with Rabbit anti-RTN3 antibody (NBP3-32921) at 1/1,000 dilution. Lane 1: HeLa cell lysate (32 ug/Lane) Lane 2: HEK-293 cell lysate (33 ug/Lane) Lane 3: U87-MG cell lysate (30 ug/Lane) Lane 4: A549 cell lysate (26 ug/Lane) Lane 5: SH-SY5Y cell lysate (18 ug/Lane) Lane 6: PC-12 cell lysate (23 ug/Lane) Lane 7: L6 cell lysate (14 ug/Lane) Lane 8: Human cerebellum tissue lysate (40 ug/Lane) Lysates/proteins at 10 ug/Lane. Predicted band size: 112.6 kDa Observed band size: 100 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32921) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: RTN3 Antibody (PSH0-40) [NBP3-32921] - Immunocytochemistry analysis of PC-12 cells labeling RTN3 with Rabbit anti-RTN3 antibody (NBP3-32921) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RTN3 antibody (NBP3-32921) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32921_rabbit-rtn3-mab-psh0-40-29202410234289.jpg)
![Flow Cytometry: RTN3 Antibody (PSH0-40) [NBP3-32921] - Flow cytometric analysis of HeLa cells labeling RTN3. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32921, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32921_rabbit-rtn3-mab-psh0-40-392024884654.jpg)
![Western Blot: RTN3 Antibody [NBP3-12988] - RTN3 antibody detects RTN3 protein by western blot analysis. HeLa whole cell extracts and membrane extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with RTN3 antibody (NBP3-12988) diluted at 1:500.](http://images.novusbio.com/fullsize/RTN3-Antibody-Western-Blot-NBP3-12988-img0001.jpg "Western Blot: RTN3 Antibody [NBP3-12988] - RTN3 antibody detects RTN3 protein by western blot analysis. HeLa whole cell extracts and membrane extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with RTN3 antibody (NBP3-12988) diluted at 1:500.")
![Immunoprecipitation: RTN3 Antibody [NBP3-12988] - Immunoprecipitation of RTN3 protein from HeLa whole cell extracts using 5 ug of RTN3 antibody (NBP3-12988). Western blot analysis was performed using RTN3 antibody (NBP3-12988) diluted at 1:500. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/RTN3-Antibody-Immunoprecipitation-NBP3-12988-img0002.jpg)
![SDS-Page: Recombinant Human RTN3 Protein [H00010313-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00010313-Q01-1.jpg "SDS-Page: Recombinant Human RTN3 Protein [H00010313-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: RTN3 Overexpression Lysate (Adult Normal) [NBP2-07679] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RTN3.](http://images.novusbio.com/fullsize/RTN3-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07679-img0001.jpg "Western Blot: RTN3 Overexpression Lysate (Adult Normal) [NBP2-07679] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RTN3.")
![Western Blot: RTN3 Antibody [H00010313-B01P] - Analysis of RTN3 expression in transfected 293T cell line by RTN3 polyclonal antibody. Lane 1: RTN3 transfected lysate(25.96 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/RTN3-Antibody-Western-Blot-H00010313-B01P-img0002.jpg "Western Blot: RTN3 Antibody [H00010313-B01P] - Analysis of RTN3 expression in transfected 293T cell line by RTN3 polyclonal antibody. Lane 1: RTN3 transfected lysate(25.96 KDa). Lane 2: Non-transfected lysate.")
![Western Blot: RTN3 Antibody [H00010313-D01P] - Analysis of RTN3 expression in transfected 293T cell line by RTN3 polyclonal antibody.Lane 1: RTN3 transfected lysate(25.60 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/RTN3-Antibody-Western-Blot-H00010313-D01P-img0001.jpg "Western Blot: RTN3 Antibody [H00010313-D01P] - Analysis of RTN3 expression in transfected 293T cell line by RTN3 polyclonal antibody.Lane 1: RTN3 transfected lysate(25.60 KDa).Lane 2: Non-transfected lysate.")