| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77211-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB5957-SP | |||
| Carrier Free | IC5957V-100UG | |||
| Carrier Free | IC5957U-100UG | |||
| Carrier Free | IC5957T-100UG | |||
| Carrier Free | IC5957S-100UG | |||
| Carrier Free | IC5957R-100UG | |||
| Carrier Free | IC5957N-100UG | |||
| Carrier Free | IC5957G-100UG | |||
![Western Blot: RPTOR Antibody [NBP2-24837] - Analysis of Raptor in A) human 293 and B) mouse NIH 3T3 lysate using Raptor antibody at 1:2000.](http://images.novusbio.com/fullsize/RPTOR-Antibody-Western-Blot-NBP2-24837-img0005.jpg "Western Blot: RPTOR Antibody [NBP2-24837] - Analysis of Raptor in A) human 293 and B) mouse NIH 3T3 lysate using Raptor antibody at 1:2000.")
![Immunocytochemistry/Immunofluorescence: RPTOR Antibody [NBP2-24837] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-RPTOR at a 1:100 overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/RPTOR-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24837-img0006.jpg)
![Immunohistochemistry-Paraffin: RPTOR Antibody [NBP2-24837] - Analysis of human small intestine using Raptor antibody at 1:100.](http://images.novusbio.com/fullsize/RPTOR-Antibody-Immunohistochemistry-Paraffin-NBP2-24837-img0004.jpg)
![Western Blot: RPTOR Antibody (9N0A4) [NBP3-16729] - Western blot analysis of extracts of various cell lines, using RPTOR Rabbit mAb (NBP3-16729) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/RPTOR-Antibody-9N0A4-Western-Blot-NBP3-16729-img0004.jpg "Western Blot: RPTOR Antibody (9N0A4) [NBP3-16729] - Western blot analysis of extracts of various cell lines, using RPTOR Rabbit mAb (NBP3-16729) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: RPTOR Antibody (9N0A4) [NBP3-16729] - Immunofluorescence analysis of NIH-3T3 cells using RPTOR Rabbit mAb (NBP3-16729) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/RPTOR-Antibody-9N0A4-Immunocytochemistry-Immunofluorescence-NBP3-16729-img0003.jpg)
![Immunohistochemistry-Paraffin: RPTOR Antibody (9N0A4) [NBP3-16729] - Immunohistochemistry of paraffin-embedded mouse kidney using RPTOR Rabbit mAb (NBP3-16729) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RPTOR-Antibody-9N0A4-Immunohistochemistry-Paraffin-NBP3-16729-img0002.jpg)
![Western Blot: RPTOR Antibody (SR1170) [NBP3-21541] - Western blot analysis using NBP3-21541 in MCF-7 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21541_rabbit-rptor-mab-sr1170-5720231552180.jpg "Western Blot: RPTOR Antibody (SR1170) [NBP3-21541] - Western blot analysis using NBP3-21541 in MCF-7 cell lysate.")
![Western Blot: RPTOR Antibody [NBP1-77211] - Analysis of RAPTOR expression in Jurkat cell lysate with RAPTOR antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/RPTOR-Antibody-Western-Blot-NBP1-77211-img0005.jpg "Western Blot: RPTOR Antibody [NBP1-77211] - Analysis of RAPTOR expression in Jurkat cell lysate with RAPTOR antibody at (A) 1 and (B) 2 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: RPTOR Antibody - BSA Free [NBP1-77211] - Immunofluorescence of RPTOR in L1210 cells with RPTOR antibody at 10 u/mL.](http://images.novusbio.com/fullsize/nbp1-77211_rabbit-polyclonal-rptor-antibody-2711202420312940.jpg)
![Immunocytochemistry/ Immunofluorescence: RPTOR Antibody - BSA Free [NBP1-77211] - Immunocytochemistry of RAPTOR in L1210 cells with RAPTOR antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77211_rabbit-polyclonal-rptor-antibody-2711202419463498.jpg)
![Western Blot: RPTOR Antibody (JE59-39) [NBP3-32894] - Western blot analysis of RPTOR on different lysates with Rabbit anti-RPTOR antibody (NBP3-32894) at 1/2,000 dilution. Lane 1: 293T cell lysate (30 ug/Lane) Lane 2: K-562 cell lysate (30 ug/Lane) Lane 3: Neuro-2a cell lysate (30 ug/Lane) Lane 4: Mouse brain tissue lysate (40 ug/Lane) Lane 5: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 149 kDa Observed band size: 149 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32894) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32894_rabbit-rptor-mab-je59-39-288202412442987.jpg "Western Blot: RPTOR Antibody (JE59-39) [NBP3-32894] - Western blot analysis of RPTOR on different lysates with Rabbit anti-RPTOR antibody (NBP3-32894) at 1/2,000 dilution. Lane 1: 293T cell lysate (30 ug/Lane) Lane 2: K-562 cell lysate (30 ug/Lane) Lane 3: Neuro-2a cell lysate (30 ug/Lane) Lane 4: Mouse brain tissue lysate (40 ug/Lane) Lane 5: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 149 kDa Observed band size: 149 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32894) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: RPTOR Antibody (JE59-39) [NBP3-32894] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-RPTOR antibody (NBP3-32894) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32894) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32894_rabbit-rptor-mab-je59-39-29820241435299.jpg)
![Western Blot: RPTOR Antibody [NB100-766] - Detection of Human Raptor by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Raptor antibody NB100-766 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/RPTOR-Antibody-Western-Blot-NB100-766-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: RPTOR Antibody [NB100-766] - Detection of Human Raptor by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Raptor antibody NB100-766 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunoprecipitation: RPTOR Antibody [NB100-766] - Detection of human Raptor by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Raptor antibody NB100-766 (lot 3) used for IP at 6 ug per reaction. Raptor was also immunoprecipitated by a previous lot of this antibody (lot 2) and rabbit anti-Raptor antibody NB100-331 For blotting immunoprecipitated Raptor, NB100-766 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/RPTOR-Antibody-Immunoprecipitation-NB100-766-img0005.jpg)
![Immunohistochemistry: RPTOR Antibody (1B10) [NBP3-15202] - IHC image of RPTOR antibody diluted at 1:100 and staining in paraffin-embedded human small intestine tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/RPTOR-Antibody-1B10-Immunohistochemistry-NBP3-15202-img0001.jpg "Immunohistochemistry: RPTOR Antibody (1B10) [NBP3-15202] - IHC image of RPTOR antibody diluted at 1:100 and staining in paraffin-embedded human small intestine tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: RPTOR Antibody (1B10) [NBP3-15202] - IHC image of RPTOR antibody diluted at 1:100 and staining in paraffin-embedded human kidney tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/RPTOR-Antibody-1B10-Immunohistochemistry-NBP3-15202-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: RPTOR Antibody [NBP2-56133] - Staining of human cell line U-2 OS shows localization to lysosomes. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/RPTOR-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56133-img0001.jpg "Immunocytochemistry/Immunofluorescence: RPTOR Antibody [NBP2-56133] - Staining of human cell line U-2 OS shows localization to lysosomes. Antibody staining is shown in green.")
![Western Blot: RPTOR [p Ser792] Antibody [NBP3-13173] - Untreated (-) and treated (+) MCF-7 whole cell extracts (60 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RPTOR (phospho Ser792) antibody (NBP3-13173) diluted at 1:1000.](http://images.novusbio.com/fullsize/RPTOR-[p-Ser792]-Antibody-Western-Blot-NBP3-13173-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: RPTOR [p Ser792] Antibody [NBP3-13173] - Untreated (-) and treated (+) MCF-7 whole cell extracts (60 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RPTOR (phospho Ser792) antibody (NBP3-13173) diluted at 1:1000.")
