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![Western Blot: PPAR delta/NR1C2 Antibody - N-terminal [NB600-637] - Affinity Purified PPAR delta/NR1C2/NR1C2 antibody is shown to detect a predominant band at ~ 40kDa corresponding to PPAR delta/NR1C2 present in mouse heart whole cell lysates. Preincubation of the antibody with the immunizing peptide completely blocks reactivity with this band (data not shown). Approximately 30ug of lysate was loaded per lane for SDS-PAGE. Detection occurred using a 1:750 dilution of primary antibody diluted in 5% BLOTTO in PBS overnight at 4 C followed reaction with a 1:10,000 dilution of IRDye(R) 800 conjugated Gt-a-Rabbit IgG (H&L) for 45 min at room temperature (800 nm channel, green). IRDye(R) 800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](http://images.novusbio.com/fullsize/PPAR-delta-NR1C2-Antibody---N-terminal-Western-Blot-NB600-637-img0007.jpg "Western Blot: PPAR delta/NR1C2 Antibody - N-terminal [NB600-637] - Affinity Purified PPAR delta/NR1C2/NR1C2 antibody is shown to detect a predominant band at ~ 40kDa corresponding to PPAR delta/NR1C2 present in mouse heart whole cell lysates. Preincubation of the antibody with the immunizing peptide completely blocks reactivity with this band (data not shown). Approximately 30ug of lysate was loaded per lane for SDS-PAGE. Detection occurred using a 1:750 dilution of primary antibody diluted in 5% BLOTTO in PBS overnight at 4 C followed reaction with a 1:10,000 dilution of IRDye(R) 800 conjugated Gt-a-Rabbit IgG (H&L) for 45 min at room temperature (800 nm channel, green). IRDye(R) 800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.")
![Immunohistochemistry: PPAR delta/NR1C2 Antibody - N-terminal [NB600-637] - Immunohistochemistry of PPAR delta/NR1C2/NR1C2 antibody. Antigen Retrieval: HIER pH 6.2. [Right] -Neg Ctrl: Normal rabbit IgG on heart striated muscle, pH6.2 (40X). [Left Top - low mag, Left Bottom - 20X] -Staining: Diffuse cytoplasmatic positivity for PPAR delta/NR1C2 in human myocardiocitys at 1:500.](http://images.novusbio.com/fullsize/PPAR-delta-NR1C2-Antibody---N-terminal-Immunohistochemistry-NB600-637-img0008.jpg)
![ELISA: PPAR delta/NR1C2 Antibody - N-terminal [NB600-637] - ELISA results of purified PPAR delta/NR1C2 antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate.](http://images.novusbio.com/fullsize/PPAR-delta-NR1C2-Antibody---N-terminal-ELISA-NB600-637-img0009.jpg)
![Immunohistochemistry of Rabbit Anti-PPAR delta (N terminal specific). Antigen Retrieval: HIER pH 6.2. [Right] -Neg Ctrl: Normal rabbit IgG on heart striated muscle, pH6.2 (40X). [Left Top - low mag, Left Bottom - 20X] -Staining: Diffuse cytoplasmatic positivity for PPAR delta in human myocardiocitys at 1:500.](http://images.novusbio.com/fullsize/nb600-637_rabbit-polyclonal-ppar-delta-nr1c2-antibody-235202318143153.jpg)
![Western Blot: PPAR delta/NR1C2 Antibody [NBP2-22468] - Analysis of 25 ug of NIH-3T3 (Lane 1), PC12 (Lane 2), and K562 (Lane 3) cell lysates and a molecular weight protein ladder.](http://images.novusbio.com/fullsize/PPAR-delta-NR1C2-Antibody-Western-Blot-NBP2-22468-img0001.jpg "Western Blot: PPAR delta/NR1C2 Antibody [NBP2-22468] - Analysis of 25 ug of NIH-3T3 (Lane 1), PC12 (Lane 2), and K562 (Lane 3) cell lysates and a molecular weight protein ladder.")
![Flow Cytometry: PPAR delta/NR1C2 Antibody [NBP2-22468] - Analysis of PPAR delta was done on jurkat cells. Cells were fixed with 70% ethanol for 10 minutes permeablized with 0.25% Triton (R) X-100 for 20 minutes and blocked with 5% BSA for 30 minutes at room temperature.](http://images.novusbio.com/fullsize/PPAR-delta-NR1C2-Antibody-Flow-Cytometry-NBP2-22468-img0003.jpg)
![Western Blot: PPAR delta/NR1C2 Antibody [NB300-962] - Staining of MOLT4 lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PPAR-delta-NR1C2-Antibody-Western-Blot-NB300-962-img0003.jpg "Western Blot: PPAR delta/NR1C2 Antibody [NB300-962] - Staining of MOLT4 lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.")
![Western Blot: PPAR delta/NR1C2 Antibody [NB300-962] - PPAR delta in HeLa cell lysate (25 ug) using anti-PPAR delta antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/PPAR-delta-NR1C2-Antibody-Western-Blot-NB300-962-img0002.jpg)
![Western Blot: PPAR delta/NR1C2 Antibody (4E3-1B11) [H00005467-M01] - PPARD monoclonal antibody (M01), clone 4E3-1B11 Analysis of PPARD expression in Jurkat.](http://images.novusbio.com/fullsize/PPAR-delta-NR1C2-Antibody-4E3-1B11-Western-Blot-H00005467-M01-img0005.jpg "Western Blot: PPAR delta/NR1C2 Antibody (4E3-1B11) [H00005467-M01] - PPARD monoclonal antibody (M01), clone 4E3-1B11 Analysis of PPARD expression in Jurkat.")
![Proximity Ligation Assay: PPAR delta/NR1C2 Antibody (4E3-1B11) [H00005467-M01] - Analysis of protein-protein interactions between HDAC2 and PPARD. HeLa cells were stained with anti-HDAC2 rabbit purified polyclonal 1:1200 and anti-PPARD mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/PPAR-delta-NR1C2-Antibody-4E3-1B11-Proximity-Ligation-Assay-H00005467-M01-img0003.jpg)
![Sandwich ELISA: PPAR delta/NR1C2 Antibody (4E3-1B11) [H00005467-M01] - Detection limit for recombinant GST tagged PPARD is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PPAR-delta-NR1C2-Antibody-4E3-1B11-Sandwich-ELISA-H00005467-M01-img0004.jpg)
![Western Blot: PPAR delta/NR1C2 Antibody (1G4) [H00005467-M03] - Analysis of PPARD expression in K-562 (Cat # L009V1).](http://images.novusbio.com/fullsize/PPAR-delta-NR1C2-Antibody-1G4-Western-Blot-H00005467-M03-img0003.jpg "Western Blot: PPAR delta/NR1C2 Antibody (1G4) [H00005467-M03] - Analysis of PPARD expression in K-562 (Cat # L009V1).")
![ELISA: PPAR delta/NR1C2 Antibody (1G4) [H00005467-M03] - Detection limit for recombinant GST tagged PPARD is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PPAR-delta-NR1C2-Antibody-1G4-ELISA-H00005467-M03-img0002.jpg)
![Western Blot: PPAR delta Overexpression Lysate (Adult Normal) [NBL1-14632] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PPAR delta.](http://images.novusbio.com/fullsize/PPAR-delta-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14632-img0002.jpg "Western Blot: PPAR delta Overexpression Lysate (Adult Normal) [NBL1-14632] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PPAR delta.")
![ELISA: Human PPAR delta/NR1C2 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39315] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39315_human-ppar-delta-nr1c2-ready-to-use-elisa-kit-color-2110202415384059.png "ELISA: Human PPAR delta/NR1C2 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39315] - Standard Curve Reference")
![ELISA: Human PPAR delta/NR1C2 ELISA Kit (Colorimetric) [NBP3-39314] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39314_human-ppar-delta-nr1c2-elisa-kit-colorimetric-211020241529564.png "ELISA: Human PPAR delta/NR1C2 ELISA Kit (Colorimetric) [NBP3-39314] - Standard Curve Reference")