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![Western Blot: Perilipin Antibody [NB110-40760] - Total protein from Human breast (lane 1), Human adipose membrane fraction (lane 2) and Human adipose tissue (lane 3) were separated on a 12% gel by SDS-PAGE. Protein was transferred to PVDF membrane, probed with 2 ug/mL anti-perilipin antibody followed by anti-rabbit HRP conjugated secondary antibody and detected with chemiluminescence.](http://images.novusbio.com/fullsize/Perilipin-Antibody-Western-Blot-NB110-40760-img0002.jpg "Western Blot: Perilipin Antibody [NB110-40760] - Total protein from Human breast (lane 1), Human adipose membrane fraction (lane 2) and Human adipose tissue (lane 3) were separated on a 12% gel by SDS-PAGE. Protein was transferred to PVDF membrane, probed with 2 ug/mL anti-perilipin antibody followed by anti-rabbit HRP conjugated secondary antibody and detected with chemiluminescence.")
![Immunohistochemistry-Paraffin: Perilipin Antibody [NB110-40760] - IHC analysis of formalin fixed paraffin embedded section of liver tissue from mouse with Perilipin antibody at 1:200 dilution. The hepatocytes developed specific staining in the cytoplasm and around the nuclei of some cells, the immunostaining reflected a punctate appearance which is potentially the ER of the cells.](http://images.novusbio.com/fullsize/Perilipin-Antibody-Immunohistochemistry-Paraffin-NB110-40760-img0005.jpg)
![Flow Cytometry: Perilipin Antibody [NB110-40760] - An intracellular stain was performed on U2OS cells with Perilipin Antibody NB110-40760AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Perilipin-Antibody-Flow-Cytometry-NB110-40760-img0008.jpg)
![Immunohistochemistry-Paraffin: Perilipin Antibody [NB110-40760] - IHC analysis of formalin fixed paraffin embedded section of fat tissue from mouse with Perilipin antibody at 1:200 dilution. The antibody generated an expected staining in the adipocytes towards periphery of the cells.](http://images.novusbio.com/fullsize/Perilipin-Antibody-Immunohistochemistry-Paraffin-NB110-40760-img0003.jpg)
![Immunohistochemistry-Paraffin: Perilipin Antibody [NB110-40760] - IHC analysis of formalin fixed paraffin embedded section of liver tissue from mouse with Perilipin antibody at 1:200 dilution. The hepatocytes developed specific staining in the cytoplasm and around the nuclei of some cells, the immunostaining reflected a punctate appearance which is potentially the ER of the cells.](http://images.novusbio.com/fullsize/Perilipin-Antibody-Immunohistochemistry-Paraffin-NB110-40760-img0006.jpg)
![Flow Cytometry: Perilipin Antibody [NB110-40760] - An intracellular stain was performed on MCF7 cells with Perilipin Antibody NB110-40760AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Perilipin-Antibody-Flow-Cytometry-NB110-40760-img0007.jpg)
![Imaging Mass Cytometry: Perilipin Antibody [NB110-40760] - Human bone marrow analyzed by IMC. Perilipin antibody conjugated with 151Eu and used 1:50 on human bone marrow FFPE. Perilipin - red. DNA- white. IMC image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Perilipin-Antibody-Imaging-Mass-Cytometry-NB110-40760-img0009.jpg)
![Western Blot: Perilipin Antibody (1K4W3) [NBP3-16480] - Western blot analysis of extracts of various cell lines, using Perilipin Rabbit mAb (NBP3-16480) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Perilipin-Antibody-1K4W3-Western-Blot-NBP3-16480-img0005.jpg "Western Blot: Perilipin Antibody (1K4W3) [NBP3-16480] - Western blot analysis of extracts of various cell lines, using Perilipin Rabbit mAb (NBP3-16480) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry: Perilipin Antibody (1K4W3) [NBP3-16480] - Immunofluorescence analysis of mouse brown adipose using Perilipin Rabbit mAb (NBP3-16480) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Perilipin-Antibody-1K4W3-Immunohistochemistry-NBP3-16480-img0004.jpg)
![Immunohistochemistry-Paraffin: Perilipin Antibody (1K4W3) [NBP3-16480] - Immunohistochemistry of paraffin-embedded rat brown adipose using Perilipin Rabbit mAb (NBP3-16480) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Perilipin-Antibody-1K4W3-Immunohistochemistry-Paraffin-NBP3-16480-img0001.jpg)
![Immunohistochemistry-Paraffin: Perilipin Antibody (1K4W3) [NBP3-16480] - Immunohistochemistry of paraffin-embedded mouse brown adipose using Perilipin Rabbit mAb (NBP3-16480) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Perilipin-Antibody-1K4W3-Immunohistochemistry-Paraffin-NBP3-16480-img0002.jpg)
![Immunohistochemistry: Perilipin Antibody (1K4W3) [NBP3-16480] - Immunofluorescence analysis of rat fat using Perilipin Rabbit mAb (NBP3-16480) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Perilipin-Antibody-1K4W3-Immunohistochemistry-NBP3-16480-img0003.jpg)
![Western Blot: Perilipin Antibody (GT2781) [NBP3-13615] - Human adipose (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Perilipin antibody [GT2781] (NBP3-13615) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.](http://images.novusbio.com/fullsize/Perilipin-Antibody-GT2781-Western-Blot-NBP3-13615-img0003.jpg "Western Blot: Perilipin Antibody (GT2781) [NBP3-13615] - Human adipose (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Perilipin antibody [GT2781] (NBP3-13615) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.")
![Immunohistochemistry-Paraffin: Perilipin Antibody (GT2781) [NBP3-13615] - Perilipin antibody [GT2781] detects Perilipin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse white adipocyte. Perilipin stained by Perilipin antibody [GT2781] (NBP3-13615) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Perilipin-Antibody-GT2781-Immunohistochemistry-Paraffin-NBP3-13615-img0002.jpg)
![Western Blot: Perilipin Antibody (GT2781) [NBP3-13615] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Perilipin antibody [GT2781] (NBP3-13615) diluted at 1:5000. The HRP-conjugated anti-mouset IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Perilipin-Antibody-GT2781-Western-Blot-NBP3-13615-img0001.jpg)
![Immunohistochemistry-Paraffin: Perilipin Antibody [NBP1-87264] - Analysis in human adipose tissue and cervix, uterine tissues using NBP1-87264 antibody. Corresponding Perilipin RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Perilipin-Antibody-Immunohistochemistry-Paraffin-NBP1-87264-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Perilipin Antibody [NBP1-87264] - Analysis in human adipose tissue and cervix, uterine tissues using NBP1-87264 antibody. Corresponding Perilipin RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Perilipin Antibody [NBP1-87264] - Staining of human breast shows high expression.](http://images.novusbio.com/fullsize/Perilipin-Antibody-Immunohistochemistry-Paraffin-NBP1-87264-img0004.jpg)
![Immunohistochemistry-Paraffin: Perilipin Antibody [NBP1-87264] - Staining of human placenta shows low expression as expected.](http://images.novusbio.com/fullsize/Perilipin-Antibody-Immunohistochemistry-Paraffin-NBP1-87264-img0005.jpg)
![Immunohistochemistry-Paraffin: Perilipin Antibody [NBP2-99070] - Immunochemical staining of human Perilipin in human liver with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Perilipin-Antibody-Immunohistochemistry-Paraffin-NBP2-99070-img0002.jpg "Immunohistochemistry-Paraffin: Perilipin Antibody [NBP2-99070] - Immunochemical staining of human Perilipin in human liver with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: Perilipin Antibody [NBP2-99070] - Immunochemical staining of human Perilipin in human breast with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Perilipin-Antibody-Immunohistochemistry-Paraffin-NBP2-99070-img0001.jpg)
![SDS-Page: Recombinant Human Perilipin Protein [H00005346-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00005346-Q01-1.jpg "SDS-Page: Recombinant Human Perilipin Protein [H00005346-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: Perilipin Antibody [NBP3-23511] - (0.3ug/ml) staining of Human Adipose tissue lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/antibody/nbp3-23511_goat-perilipin-pab-149202382335..jpg "Western Blot: Perilipin Antibody [NBP3-23511] - (0.3ug/ml) staining of Human Adipose tissue lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Western Blot: Perilipin Overexpression Lysate (Adult Normal) [NBP2-11344] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Perilipin.](http://images.novusbio.com/fullsize/Perilipin-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-11344-img0001.jpg "Western Blot: Perilipin Overexpression Lysate (Adult Normal) [NBP2-11344] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Perilipin.")
![Western Blot: Perilipin Overexpression Lysate (Adult Normal) [NBP2-10847] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Perilipin.](http://images.novusbio.com/fullsize/Perilipin-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10847-img0001.jpg "Western Blot: Perilipin Overexpression Lysate (Adult Normal) [NBP2-10847] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Perilipin.")
![ELISA: Perilipin Antibody (4G1) [H00005346-M09] - Detection limit for recombinant GST tagged PLIN is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Perilipin-Antibody-4G1-ELISA-H00005346-M09-img0001.jpg "ELISA: Perilipin Antibody (4G1) [H00005346-M09] - Detection limit for recombinant GST tagged PLIN is approximately 3ng/ml as a capture antibody.")