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![Western Blot: PHGDH Antibody [NBP1-87311] - Analysis in human cell lines SK-MEL-30 and A-549. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Western-Blot-NBP1-87311-img0018.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: PHGDH Antibody [NBP1-87311] - Analysis in human cell lines SK-MEL-30 and A-549. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: PHGDH Antibody [NBP1-87311] - Staining of human cell line U-2 OS shows localization to plasma membrane & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87311-img0016.jpg)
![Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-87311] - Immunohistochemical staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neuronal and glial cells.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Immunohistochemistry-Paraffin-NBP1-87311-img0013.jpg)
![Western Blot: PHGDH Antibody [NBP1-87311] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Western-Blot-NBP1-87311-img0015.jpg)
![Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-87311] - Immunohistochemical staining of human skeletal muscle shows no positivity as expected.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Immunohistochemistry-Paraffin-NBP1-87311-img0010.jpg)
![Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-87311] - Immunohistochemical staining of human breast cancer shows moderate to strong cytoplasmic positivity in tumor cells.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Immunohistochemistry-Paraffin-NBP1-87311-img0011.jpg)
![Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-87311] - Immunohistochemical staining of human skin shows moderate cytoplasmic positivity in epidermal cells.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Immunohistochemistry-Paraffin-NBP1-87311-img0012.jpg)
![Simple Western: PHGDH Antibody [NBP1-87311] - Simple Western lane view shows a specific band for PHGDH in 0.2 mg/ml of RT-4 (Left) and U-251MG (Right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Simple-Western-NBP1-87311-img0007.jpg)
![Simple Western: PHGDH Antibody [NBP1-87311] - Electropherogram image(s) of corresponding Simple Western lane view. PHGDH antibody was used at 1:20 dilution on RT-4 and U-251MG lysate(s).](http://images.novusbio.com/fullsize/PHGDH-Antibody-Simple-Western-NBP1-87311-img0008.jpg)
![Western Blot: PHGDH Antibody [NBP1-87311] - Knockdown of PNKP or TDP1 leads to increase in expression of PSAT1 & PHGDH. Western blot analysis of XRCC1, PHGDH & PSAT1 expression upon KD of PNKP (A) or TDP1 (B). beta -Actin served as a loading control. (C) TDP1 mRNA levels after KD of TDP1, quantified by qPCR & normalised to GAPDH. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25800737), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-87311_rabbit-polyclonal-phgdh-antibody-310202415395946.jpg)
![Knockout Validated: PHGDH Antibody [NBP1-32201] - Wild-type (WT) and PHGDH knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PHGDH antibody [N1N2], N-term. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Western-Blot-NBP1-32201-img0013.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: PHGDH Antibody [NBP1-32201] - Wild-type (WT) and PHGDH knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PHGDH antibody [N1N2], N-term. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: PHGDH Antibody [NBP1-32201] - Sample(30 ug of whole cell lysate)A:293TB:A431 C:HeLa S3 7. 5% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Western-Blot-NBP1-32201-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: PHGDH Antibody [NBP1-32201] - PHGDH antibody detects PHGDH protein by immunohistochemical analysis. Sample: Frozen-sectioned rat E13.5 brain. Green: PHGDH stained by PHGDH antibody diluted at 1:250. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32201-img0014.jpg)
![Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-32201] - Paraffin-embedded mouse pancreas. PHGDH antibody [N1N2], N-term diluted at 1:500.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Immunohistochemistry-Paraffin-NBP1-32201-img0008.jpg)
![Western Blot: PHGDH Antibody [NBP1-32201] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Western-Blot-NBP1-32201-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: PHGDH Antibody [NBP1-32201] - Analysis of methanol-fixed HeLa, using PHGDH antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32201-img0012.jpg)
![Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-32201] - Paraffin-embedded PC13 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Immunohistochemistry-Paraffin-NBP1-32201-img0005.jpg)
![Immunoprecipitation: PHGDH Antibody [NBP1-32201] - Immunoprecipitation of PHGDH protein from 293T whole cell extracts using 5 ug of PHGDH antibody [N1N2], N-term (NBP1-32201). Western blot analysis was performed using PHGDH antibody [N1N2], N-term (NBP1-32201). EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Immunoprecipitation-NBP1-32201-img0011.jpg)
![Western Blot: PHGDH Antibody (4A3-1D6) [H00026227-M01] - PHGDH monoclonal antibody (M01), clone 4A3-1D6. Analysis of PHGDH expression in human liver.](http://images.novusbio.com/fullsize/PHGDH-Antibody-4A3-1D6-Western-Blot-H00026227-M01-img0018.jpg "Western Blot: PHGDH Antibody (4A3-1D6) [H00026227-M01] - PHGDH monoclonal antibody (M01), clone 4A3-1D6. Analysis of PHGDH expression in human liver.")
![Immunocytochemistry/Immunofluorescence: PHGDH Antibody (4A3-1D6) [H00026227-M01] - Analysis of monoclonal antibody to PHGDH on HeLa cell. Antibody concentration 1 ~ 10 ug/ml.](http://images.novusbio.com/fullsize/PHGDH-Antibody-4A3-1D6-Immunocytochemistry-Immunofluorescence-H00026227-M01-img0010.jpg)
![Immunohistochemistry-Paraffin: PHGDH Antibody (4A3-1D6) [H00026227-M01] - Analysis of monoclonal antibody to PHGDH on formalin-fixed paraffin-embedded human prostate. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/PHGDH-Antibody-4A3-1D6-Immunohistochemistry-Paraffin-H00026227-M01-img0014.jpg)
![Western Blot: PHGDH Antibody (4A3-1D6) [H00026227-M01] - PHGDH monoclonal antibody (M01), clone 4A3-1D6. Analysis of PHGDH expression in A-431.](http://images.novusbio.com/fullsize/PHGDH-Antibody-4A3-1D6-Western-Blot-H00026227-M01-img0016.jpg)
![Western Blot: PHGDH Antibody (4A3-1D6) [H00026227-M01] - PHGDH monoclonal antibody (M01), clone 4A3-1D6 Analysis of PHGDH expression in Jurkat.](http://images.novusbio.com/fullsize/PHGDH-Antibody-4A3-1D6-Western-Blot-H00026227-M01-img0017.jpg)
![Western Blot: PHGDH Antibody (4A3-1D6) [H00026227-M01] - Analysis of PHGDH expression in transfected 293T cell line by PHGDH monoclonal antibody (M01), clone 4A3-1D6.Lane 1: PHGDH transfected lysate(56.7 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PHGDH-Antibody-4A3-1D6-Western-Blot-H00026227-M01-img0019.jpg)
![Immunohistochemistry-Paraffin: PHGDH Antibody (4A3-1D6) [H00026227-M01] - Analysis of monoclonal antibody to PHGDH on formalin-fixed paraffin-embedded human lymph node. Antibody concentration 1 ~ 10 ug/ml.](http://images.novusbio.com/fullsize/PHGDH-Antibody-4A3-1D6-Immunohistochemistry-Paraffin-H00026227-M01-img0012.jpg)
![Immunoprecipitation: PHGDH Antibody (4A3-1D6) [H00026227-M01] - Analysis of PHGDH transfected lysate using anti-PHGDH monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PHGDH MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/PHGDH-Antibody-4A3-1D6-Immunoprecipitation-H00026227-M01-img0015.jpg)
![Western Blot: PHGDH Antibody (CL0555) [NBP2-52939] - Lane 1: Marker [kDa], Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/PHGDH-Antibody-CL0555-Western-Blot-NBP2-52939-img0008.jpg "Western Blot: PHGDH Antibody (CL0555) [NBP2-52939] - Lane 1: Marker [kDa], Lane 2: Human cell line RT-4")
![Immunocytochemistry/Immunofluorescence: PHGDH Antibody (CL0555) [NBP2-52939] - Staining of MCF7 cells using the Anti-PHGDH monoclonal antibody, showing specific staining in the cytosol and the plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/PHGDH-Antibody-CL0555-Immunocytochemistry-Immunofluorescence-NBP2-52939-img0009.jpg)
![Western Blot: PHGDH Antibody (CL0555) [NBP2-52939] - Lane 1: Marker [kDa], Lane 2: Human cell line HeLa cytoplasmic fraction , Lane 3: Human cell line HeLa membrane fraction , Lane 4: Human cell line HeLa nuclear fraction , Lane 5: Human cell line HeLa chromatin fraction , Lane 6: Human cell line HeLa cytos](http://images.novusbio.com/fullsize/PHGDH-Antibody-CL0555-Western-Blot-NBP2-52939-img0007.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PHGDH Antibody (CL0555) [NBP2-52939] -Analysis in human pancreas and skeletal muscle tissues using NBP2-52939 antibody. Corresponding PHGDH RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/antibody/nbp2-52939_mouse-monoclonal-phgdh-antibody-cl0555-immunohistochemistry-paraffin-188202393027.jpg)
![Immunohistochemistry-Paraffin: PHGDH Antibody (CL0555) [NBP2-52939] -Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp2-52939_mouse-monoclonal-phgdh-antibody-cl0555-immunohistochemistry-paraffin-188202393135..jpg)
![Immunohistochemistry-Paraffin: PHGDH Antibody (CL0555) [NBP2-52939] -Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/antibody/nbp2-52939_mouse-monoclonal-phgdh-antibody-cl0555-immunohistochemistry-paraffin-188202393244..jpg)
![Immunohistochemistry-Paraffin: PHGDH Antibody (CL0555) [NBP2-52939] -Staining of human skin shows strong cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/antibody/nbp2-52939_mouse-monoclonal-phgdh-antibody-cl0555-immunohistochemistry-paraffin-188202393356..jpg)
![Immunohistochemistry-Paraffin: PHGDH Antibody (CL0555) [NBP2-52939] -Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/antibody/nbp2-52939_mouse-monoclonal-phgdh-antibody-cl0555-immunohistochemistry-paraffin-18820239356..jpg)
![Knockout Validated: PHGDH Antibody [NBP2-19801] - Wild-type (WT) and PHGDH knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PHGDH antibody [N3C2], Internal. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Western-Blot-NBP2-19801-img0003.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: PHGDH Antibody [NBP2-19801] - Wild-type (WT) and PHGDH knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PHGDH antibody [N3C2], Internal. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: PHGDH Antibody [NBP2-19801] - Sample (30 ug of whole cell lysate) A: A431 B: Raji 7. 5% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Western-Blot-NBP2-19801-img0001.jpg)
![Immunohistochemistry-Frozen: PHGDH Antibody [NBP2-19801] - Frozen-sectioned rat E13.5 brain. Green: PHGDH stained by PHGDH antibody [N3C2], Internal diluted at 1:250. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Immunohistochemistry-Frozen-NBP2-19801-img0004.jpg)
![Immunohistochemistry-Paraffin: PHGDH Antibody [NBP2-19801] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Immunohistochemistry-Paraffin-NBP2-19801-img0002.jpg)
![Western Blot: PHGDH Antibody [NBP1-87310] - Analysis using Anti-PHGDH antibody NBP1-87310 (A) shows similar pattern to independent antibody NBP1-87311 (B).](http://images.novusbio.com/fullsize/PHGDH-Antibody-Western-Blot-NBP1-87310-img0011.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PHGDH Antibody [NBP1-87310] - Analysis using Anti-PHGDH antibody NBP1-87310 (A) shows similar pattern to independent antibody NBP1-87311 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: PHGDH Antibody [NBP1-87310] - Staining of human cell line MCF7 shows localization to nucleoplasm, plasma membrane & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87310-img0007.jpg)
![Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-87310] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Immunohistochemistry-Paraffin-NBP1-87310-img0013.jpg)
![Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-87310] - Staining of human cervix, uterine shows nuclear and cytoplasmic positivity in squamous cells.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Immunohistochemistry-Paraffin-NBP1-87310-img0009.jpg)
![Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-87310] - Staining of human cerebral cortex shows strong cytoplasmic positivity in glial cells.](http://images.novusbio.com/fullsize/PHGDH-Antibody-Immunohistochemistry-Paraffin-NBP1-87310-img0012.jpg)
![Western Blot: PHGDH Antibody (5C4) [NBP2-46389] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PHGDH.](http://images.novusbio.com/fullsize/PHGDH-Antibody-5C4-Western-Blot-NBP2-46389-img0002.jpg "Western Blot: PHGDH Antibody (5C4) [NBP2-46389] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PHGDH.")
![Immunohistochemistry: PHGDH Antibody (5C4) [NBP2-46389] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120 degrees C for 3min)](http://images.novusbio.com/fullsize/PHGDH-Antibody-5C4-Immunohistochemistry-NBP2-46389-img0001.jpg)
![Immunohistochemistry;PHGDH Antibody[NBP3-29511]Detection of human PHGDH by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-PHGDH used at a dilution of 1:1,000 . Detection: DAB](http://images.novusbio.com/fullsize/nbp3-29535_rabbit-phgdh-pab-78202410153528.jpg "Immunohistochemistry;PHGDH Antibody[NBP3-29511]Detection of human PHGDH by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-PHGDH used at a dilution of 1:1,000 . Detection: DAB")
![Immunohistochemistry-Paraffin: PHGDH Antibody [NBP3-18850] - Immunochemical staining of human PHGDH in human skin with NBP3-18850 at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-18850_rabbit-phgdh-pab-136202422345037.png "Immunohistochemistry-Paraffin: PHGDH Antibody [NBP3-18850] - Immunochemical staining of human PHGDH in human skin with NBP3-18850 at 1:100 dilution, formalin-fixed paraffin embedded sections.")
![Immunocytochemistry/ Immunofluorescence: PHGDH Antibody [NBP3-18850] - Immunofluorescence staining of PHGDH in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP3-18850 (dilution ratio 1:100) at 4C overnight. Then cells were stained with the Goat Anti-rabbit IgG secondary antibody [Alexa Fluor 488] (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp3-18850_rabbit-phgdh-pab-13620242234503.png)
![Immunohistochemistry-Paraffin: PHGDH Antibody [NBP3-18850] - Immunochemical staining of human PHGDH in human breast carcinoma with NBP3-18850 at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-18850_rabbit-phgdh-pab-136202422345032.png)
![Western Blot: PHGDH Overexpression Lysate (Adult Normal) [NBL1-14363] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PHGDH.](http://images.novusbio.com/fullsize/PHGDH-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14363-img0002.jpg "Western Blot: PHGDH Overexpression Lysate (Adult Normal) [NBL1-14363] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PHGDH.")
![Western Blot: PHGDH Antibody (4D5-1F11) [H00026227-M02] - Detection against Immunogen (84.37 KDa) .](http://images.novusbio.com/fullsize/PHGDH-Antibody-4D5-1F11-Western-Blot-H00026227-M02-img0001.jpg "Western Blot: PHGDH Antibody (4D5-1F11) [H00026227-M02] - Detection against Immunogen (84.37 KDa) .")