Note: Mouseover a species abbreviation on the product page to display the fullname.
How can we help you?
![Western Blot: Pax6 Antibody [NBP2-19711] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NBP2-19711-img0023.jpg "Western Blot: Pax6 Antibody [NBP2-19711] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Pax6 Antibody [NBP2-19711] - SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PAX6 protein stained by PAX6 antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:50. Scale bar = 10 um.](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19711-img0013.jpg)
![Immunohistochemistry-Paraffin: Pax6 Antibody [NBP2-19711] - Mouse retina. Green: PAX6 protein stained by PAX6 antibody diluted at 1:250. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [1338] diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunohistochemistry-Paraffin-NBP2-19711-img0021.jpg)
![Western Blot: Pax6 Antibody [NBP2-19711] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NBP2-19711-img0015.jpg)
![Western Blot: Pax6 Antibody [NBP2-19711] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NBP2-19711-img0017.jpg)
![Immunohistochemistry-Frozen: Pax6 Antibody [NBP2-19711] - Frozen sectioned E13.5 Rat brain. Green: PAX6 protein stained by PAX6 antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunohistochemistry-Frozen-NBP2-19711-img0010.jpg)
![Immunohistochemistry-Frozen: Pax6 Antibody [NBP2-19711] - Frozen section of embryonic mouse brain (mE12.5). PAX6 antibody diluted at 1:250.](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunohistochemistry-Frozen-NBP2-19711-img0012.jpg)
![Immunoprecipitation: PAX6 Antibody [NBP2-19711] - Sample: 1000 ug 293T whole cell lysate/extract A. 50 ug 293T whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of PAX6 protein by 2 ug of PAX6 antibody 10% SDS-PAGE The immunoprecipitated PAX6 protein was detected by PAX6 antibody diluted at 1:1000. anti-rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/PAX6-Antibody-Immunoprecipitation-NBP2-19711-img0004.jpg)
![Immunohistochemistry-Paraffin: Pax6 Antibody [NBP2-19711] - Pax6 antibody detects Pax6 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse E10.5 embryo.Green: Pax6 stained by Pax6 antibody (NBP2-19711) diluted at 1:250.Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-19711_rabbit-polyclonal-pax6-antibody-910202420104458.jpg)
![Immunohistochemistry-Paraffin: Pax6 Antibody [NBP2-19711] - Pax6 antibody detects Pax6 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat cerebellum.Pax6 stained by Pax6 antibody (NBP2-19711) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-19711_rabbit-polyclonal-pax6-antibody-910202419593540.jpg)
![Immunocytochemistry/ Immunofluorescence: Pax6 Antibody [NBP2-19711] - Pax6 antibody detects Pax6 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Pax6 stained by Pax6 antibody (NBP2-19711) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-19711_rabbit-polyclonal-pax6-antibody-910202420115034.jpg)
![Immunohistochemistry-Paraffin: Pax6 Antibody [NBP2-19711] - Pax6 antibody detects Pax6 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat eye.Green: Pax6 stained by Pax6 antibody (NBP2-19711) diluted at 1:250.Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-19711_rabbit-polyclonal-pax6-antibody-91020242075957.jpg)
![Immunohistochemistry-Paraffin: Pax6 Antibody [NBP2-19711] - Pax6 antibody detects Pax6 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse cerebellum.Pax6 stained by Pax6 antibody (NBP2-19711) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-19711_rabbit-polyclonal-pax6-antibody-91020242001050.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Pax6 Antibody [NBP2-19711] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-19711_rabbit-polyclonal-pax6-antibody-161020241941454.jpg)
![Immunocytochemistry/Immunofluorescence: Pax6 Antibody [NBP1-89100] - Staining of human cell line HEK 293 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89100-img0007.jpg "Immunocytochemistry/Immunofluorescence: Pax6 Antibody [NBP1-89100] - Staining of human cell line HEK 293 shows localization to nucleoplasm. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Pax6 Antibody [NBP1-89100] - Staining of human endometrium shows no positivity as expected.](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunohistochemistry-Paraffin-NBP1-89100-img0012.jpg)
![Immunohistochemistry-Paraffin: Pax6 Antibody [NBP1-89100] - Staining of human pancreas shows strong nuclear positivity in islet cells.](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunohistochemistry-Paraffin-NBP1-89100-img0005.jpg)
![Immunohistochemistry-Paraffin: Pax6 Antibody [NBP1-89100] - Staining of human cerebellum shows moderate to strong nuclear positivity in cells in granular layer](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunohistochemistry-Paraffin-NBP1-89100-img0009.jpg)
![Immunohistochemistry-Paraffin: Pax6 Antibody [NBP1-89100] - Staining of human stomach shows strong nuclear positivity in a subset glandular cells.](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunohistochemistry-Paraffin-NBP1-89100-img0011.jpg)
![Western Blot: Pax6 Antibody [NB300-750] - Analysis of sheep retinal extract](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NB300-750-img0023.jpg "Western Blot: Pax6 Antibody [NB300-750] - Analysis of sheep retinal extract")
![Immunocytochemistry/Immunofluorescence: Pax6 Antibody [NB300-750] - ARPE-19 monolayer on glass substrate. Fixative Methanol Blocking Goat Serum. Dilution 1:100. Incubation 1h @ RT. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunocytochemistry-NB300-750-img0024.jpg)
![Western Blot: Pax6 Antibody [NB300-750] - Analysis of rat whole eye extract](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NB300-750-img0017.jpg)
![Western Blot: Pax6 Antibody [NB300-750] - Analysis of various human, mouse and non-human primate whole cell lysates.](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NB300-750-img0016.jpg)
![Western Blot: Pax6 Antibody [NB300-750] - Analysis of extracts from HeLa, U251 and mouse brain using PAX6 antibody (NB300-750, 1:100).](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NB300-750-img0019.jpg)
![Western Blot: Pax6 Antibody [NB300-750] - Analysis of Pax6 in skate (Leucoraja erinacea) brain lysate using anti-Pax6 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NB300-750-img0022.jpg)
![Immunocytochemistry/Immunofluorescence: Pax6 Antibody [NB300-750] - Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a PAX6 polyclonal antibody at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunocytochemistry-Immunofluorescence-NB300-750-img0011.jpg)
![Immunoprecipitation: Pax6 Antibody [NB300-750] - Analysis of PAX6 was performed on 293T cells. Antigen: antibody complexes were formed by incubating 500ug whole cell lysate with 5ug of PAX6 polyclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a PAX6 monoclonal antibody at a dilution of 1:1000 overnight rotating at 4C, washed in TBST, and probed with Clean-Blot IP Detection Reagent at a dilution of 1:1000 for at least one hour.](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunoprecipitation-NB300-750-img0020.jpg)
![Immunocytochemistry/ Immunofluorescence: Pax6 Antibody [NB300-750] - Stepwise differentiation of human iPSCs towards renal progenitor cells (RPCs).(a) Schematic description of the two-stage protocol applied to iPSC-renal commitment. (b, c, d, e) Immunofluorescence of iPSCs (derived from retroviral transfected dermal fibroblasts) exposed to differentiating media. (b) The pluripotency markers SSEA4, TRA-1-81, Nanog, ME marker T(Bry) & IM marker Osr1. (c) IM & MM marker expression as Wt1, Pax8, Pax2, Six2 & Sall1. (d) Renal progenitor markers CD133, CD24, NCAM, glomerular epithelial marker Claudin1 & proximal tubular epithelial markers AQP1, GGT1. (e) Markers identifying endodermal AFP, ectodermal Pax6, & cardiac mesodermal Nkx2.5 derivation. Nuclei are stained with DAPI (blue). Scale bars: 20 μm (b, c, d, e). Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep08826), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-750_rabbit-polyclonal-pax6-antibody-3102024161898.jpg)
![SDS-Page: Recombinant Human Pax6 Protein [H00005080-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00005080-P01-1.jpg "SDS-Page: Recombinant Human Pax6 Protein [H00005080-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Immunocytochemistry/Immunofluorescence: Pax6 Antibody (CL5414) [NBP2-61411] - Staining of HEK 293 cells using the Anti-PAX6 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Pax6-Antibody-CL5414-Immunocytochemistry-Immunofluorescence-NBP2-61411-img0001.jpg "Immunocytochemistry/Immunofluorescence: Pax6 Antibody (CL5414) [NBP2-61411] - Staining of HEK 293 cells using the Anti-PAX6 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Pax6 Antibody (CL5414) [NBP2-61411] - Staining of rat eye retina shows strong nuclear positivity in the inner nuclear layer and ganglion cell layer.](http://images.novusbio.com/fullsize/Pax6-Antibody-CL5414-Immunohistochemistry-Paraffin-NBP2-61411-img0007.jpg)
![Immunohistochemistry-Paraffin: Pax6 Antibody (CL5414) [NBP2-61411] - Staining of mouse embryo E11 shows strong nuclear positivity in the developing brain and sensory organs.](http://images.novusbio.com/fullsize/Pax6-Antibody-CL5414-Immunohistochemistry-Paraffin-NBP2-61411-img0005.jpg)
![Immunohistochemistry-Paraffin: Pax6 Antibody (CL5414) [NBP2-61411] - Staining of mouse embryo E14 shows strong nuclear positivity in the developing eye.](http://images.novusbio.com/fullsize/Pax6-Antibody-CL5414-Immunohistochemistry-Paraffin-NBP2-61411-img0006.jpg)
![Western Blot: Pax6 Antibody (GT9412) [NBP3-13636] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Pax6 antibody [GT9412] (NBP3-13636) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/Pax6-Antibody-GT9412-Western-Blot-NBP3-13636-img0003.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Pax6 Antibody (GT9412) [NBP3-13636] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Pax6 antibody [GT9412] (NBP3-13636) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Pax6 Antibody (GT9412) [NBP3-13636] - Pax6 antibody [GT9412] detects Pax6 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse eye. Green: Pax6 stained by Pax6 antibody [GT9412] (NBP3-13636) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:250. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Pax6-Antibody-GT9412-Immunohistochemistry-Paraffin-NBP3-13636-img0002.jpg)
![Immunohistochemistry-Paraffin: Pax6 Antibody (GT9412) [NBP3-13636] - Pax6 antibody [GT9412] detects Pax6 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat cerebellum. Pax6 stained by Pax6 antibody [GT9412] (NBP3-13636) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Pax6-Antibody-GT9412-Immunohistochemistry-Paraffin-NBP3-13636-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: Pax6 Antibody (GT9412) [NBP3-13636] - Pax6 antibody [GT9412] detects Pax6 protein at nucleus by immunofluorescent analysis.Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Pax6 stained by Pax6 antibody [GT9412] (NBP3-13636) diluted at 1:500.Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp3-13636_mouse-pax6-mab-gt9412-91020242011450.jpg)
![Immunohistochemistry: Pax6 Antibody (22C3) [NBP3-26437] - Image of Pax6 Antibody (22C3) diluted at 1:100 and staining in paraffin-embedded human pancreati tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.55% DAB.](http://images.novusbio.com/fullsize/nbp3-26437_rabbit-pax6-mab-22c3-262202414225221.jpg "Immunohistochemistry: Pax6 Antibody (22C3) [NBP3-26437] - Image of Pax6 Antibody (22C3) diluted at 1:100 and staining in paraffin-embedded human pancreati tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.55% DAB.")
![Immunohistochemistry: Pax6 Antibody (22C3) [NBP3-26437] - Image of Pax6 Antibody (22C3) diluted at 1:100 and staining in paraffin-embedded human pancreati tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.55% DAB.](http://images.novusbio.com/fullsize/nbp3-26437_rabbit-pax6-mab-22c3-262202415475614.jpg)
![Flow Cytometry: Pax6 Antibody (22C3) [NBP3-26437] - Overlay Peak curve showing SH-SY5Y cells stained with Pax6 Antibody (22C3) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26437_rabbit-pax6-mab-22c3-282202414312722.jpg)
![Western Blot: Pax6 Antibody (S03-8G4) [NBP3-19729] - Western blot detection of Pax6 in Rat Brain lysates using NBP3-19729 (1:1000 diluted). Predicted band size: 47kDa. Observed band size: 47kDa.](http://images.novusbio.com/fullsize/Pax6-Antibody-S03-8G4-Western-Blot-NBP3-19729-img0001.jpg "Western Blot: Pax6 Antibody (S03-8G4) [NBP3-19729] - Western blot detection of Pax6 in Rat Brain lysates using NBP3-19729 (1:1000 diluted). Predicted band size: 47kDa. Observed band size: 47kDa.")
![Western Blot: Pax6 Antibody (S01-1G8) [NBP3-19528] - Western blot detection of Pax6 in K562, Hela cell lysates using NBP3-19528 (1:1000 diluted). Predicted band size: 47kDa. Observed band size: 47kDa.](http://images.novusbio.com/fullsize/Pax6-Antibody-S01-1G8-Western-Blot-NBP3-19528-img0001.jpg "Western Blot: Pax6 Antibody (S01-1G8) [NBP3-19528] - Western blot detection of Pax6 in K562, Hela cell lysates using NBP3-19528 (1:1000 diluted). Predicted band size: 47kDa. Observed band size: 47kDa.")
![Western Blot: PAX6 Overexpression Lysate (Adult Normal) [NBP2-08189] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PAX6.](http://images.novusbio.com/fullsize/PAX6-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-08189-img0001.jpg "Western Blot: PAX6 Overexpression Lysate (Adult Normal) [NBP2-08189] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PAX6.")
