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![Western Blot: PABPN1 Antibody (7V4X8) [NBP3-15539] - Western blot analysis of extracts of various cell lines, using PABPN1 Rabbit mAb (NBP3-15539) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/PABPN1-Antibody-7V4X8-Western-Blot-NBP3-15539-img0007.jpg "Western Blot: PABPN1 Antibody (7V4X8) [NBP3-15539] - Western blot analysis of extracts of various cell lines, using PABPN1 Rabbit mAb (NBP3-15539) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: PABPN1 Antibody (7V4X8) [NBP3-15539] - Immunofluorescence analysis of U-2 OS cells using PABPN1 Rabbit mAb (NBP3-15539) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PABPN1-Antibody-7V4X8-Immunocytochemistry-Immunofluorescence-NBP3-15539-img0006.jpg)
![Immunohistochemistry-Paraffin: PABPN1 Antibody (7V4X8) [NBP3-15539] - Immunohistochemistry of paraffin-embedded mouse testis using PABPN1 Rabbit mAb (NBP3-15539) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PABPN1-Antibody-7V4X8-Immunohistochemistry-Paraffin-NBP3-15539-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: PABPN1 Antibody (7V4X8) [NBP3-15539] - Immunofluorescence analysis of C6 cells using PABPN1 Rabbit mAb (NBP3-15539) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PABPN1-Antibody-7V4X8-Immunocytochemistry-Immunofluorescence-NBP3-15539-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: PABPN1 Antibody (7V4X8) [NBP3-15539] - Immunofluorescence analysis of NIH-3T3 cells using PABPN1 Rabbit mAb (NBP3-15539) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PABPN1-Antibody-7V4X8-Immunocytochemistry-Immunofluorescence-NBP3-15539-img0005.jpg)
![Immunohistochemistry-Paraffin: PABPN1 Antibody (7V4X8) [NBP3-15539] - Immunohistochemistry of paraffin-embedded rat brain using PABPN1 Rabbit mAb (NBP3-15539) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PABPN1-Antibody-7V4X8-Immunohistochemistry-Paraffin-NBP3-15539-img0001.jpg)
![Immunohistochemistry-Paraffin: PABPN1 Antibody (7V4X8) [NBP3-15539] - Immunohistochemistry of paraffin-embedded human esophageal using PABPN1 Rabbit mAb (NBP3-15539) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PABPN1-Antibody-7V4X8-Immunohistochemistry-Paraffin-NBP3-15539-img0002.jpg)
![Western Blot: PABPN1 Antibody [NBP1-31805] - Sample (30 ug of whole cell lysate) A: Jurkat 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/PABPN1-Antibody-Western-Blot-NBP1-31805-img0006.jpg "Western Blot: PABPN1 Antibody [NBP1-31805] - Sample (30 ug of whole cell lysate) A: Jurkat 12% SDS PAGE; antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: PABPN1 Antibody [NBP1-31805] - A375 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PABPN1 protein stained by PABPN1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/PABPN1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31805-img0008.jpg)
![Immunohistochemistry-Paraffin: PABPN1 Antibody [NBP1-31805] - Human colon carcinoma, using PABPN1 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/PABPN1-Antibody-Immunohistochemistry-Paraffin-NBP1-31805-img0004.jpg)
![Western Blot: PABPN1 Antibody [NBP1-31805] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/PABPN1-Antibody-Western-Blot-NBP1-31805-img0005.jpg)
![Immunohistochemistry: PABPN1 Antibody (6C3) [NBP3-26444] - Image of PABPN1 Antibody (6C3) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26444_rabbit-pabpn1-mab-6c3-26220241522423.jpg "Immunohistochemistry: PABPN1 Antibody (6C3) [NBP3-26444] - Image of PABPN1 Antibody (6C3) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: PABPN1 Antibody (6C3) [NBP3-26444] - Image of PABPN1 Antibody (6C3) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26444_rabbit-pabpn1-mab-6c3-26220241547568.jpg)
![Western Blot: PABPN1 Antibody (6C3) [NBP3-26444] - Positive Western Blot detected in: 293 whole cell lysate, MCF-7 whole cell lysate, Raji whole cell lysate, HepG2 whole cell lysate. All lanes: PABPN1 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 33, 32, 38 kDa Observed band size: 50 kDa](http://images.novusbio.com/fullsize/nbp3-26444_rabbit-pabpn1-mab-6c3-282202411413621.jpg)
![Immunocytochemistry/Immunofluorescence: PABPN1 Antibody (6C3) [NBP3-26444] - Staining of Hela Cells with PABPN1 Antibody (6C3) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26444_rabbit-pabpn1-mab-6c3-282202412475117.jpg)
![Flow Cytometry: PABPN1 Antibody (6C3) [NBP3-26444] - Overlay histogram showing Hela cells stained with PABPN1 Antibody (6C3) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26444_rabbit-pabpn1-mab-6c3-282202414312748.jpg)
![Western Blot: PABPN1 Antibody [NBP1-82481] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/PABPN1-Antibody-Western-Blot-NBP1-82481-img0005.jpg "Western Blot: PABPN1 Antibody [NBP1-82481] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Mouse Cerebral Cortex tissue")
![Immunohistochemistry-Paraffin: PABPN1 Antibody [NBP1-82481] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/PABPN1-Antibody-Immunohistochemistry-Paraffin-NBP1-82481-img0007.jpg)
![Western Blot: PABPN1 Antibody [NBP1-82481] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/PABPN1-Antibody-Western-Blot-NBP1-82481-img0004.jpg)
![Western Blot: PABPN1 Antibody [NBP1-57216] - N-terminal region validated by WB using C2C12 myoblast cell line at 1.25 ug/ml.](http://images.novusbio.com/fullsize/PABPN1-Antibody-Western-Blot-NBP1-57216-img0006.jpg "Western Blot: PABPN1 Antibody [NBP1-57216] - N-terminal region validated by WB using C2C12 myoblast cell line at 1.25 ug/ml.")
![Immunocytochemistry/Immunofluorescence: PABPN1 Antibody [NBP1-57216] - C2C12 myoblast cell line.](http://images.novusbio.com/fullsize/PABPN1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-57216-img0007.jpg)
![Western Blot: PABPN1 Antibody [NBP1-57216] - C2C12 myoblast cell line, Antibody Titration: 0.2-1 ug/ml](http://images.novusbio.com/fullsize/PABPN1-Antibody-Western-Blot-NBP1-57216-img0005.jpg)
![Western Blot: PABPN1 Antibody [NBP2-93664] - Analysis of extracts of various cell lines, using PABPN1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.](http://images.novusbio.com/fullsize/PABPN1-Antibody-Western-Blot-NBP2-93664-img0001.jpg "Western Blot: PABPN1 Antibody [NBP2-93664] - Analysis of extracts of various cell lines, using PABPN1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.")
![SDS-Page: Recombinant Human PABPN1 Protein [NBP2-51516] - 15% SDS Page (3 ug)](http://images.novusbio.com/fullsize/PABPN1-Recombinant-Protein-SDS-Page-NBP2-51516-img0002.jpg "SDS-Page: Recombinant Human PABPN1 Protein [NBP2-51516] - 15% SDS Page (3 ug)")