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![Western Blot: PABP Antibody (10E10) [NB120-6125] - Analysis of PABP expression in 1) A-431, 2) HeLa and 3) NIH-3T3 whole cell lysates.](http://images.novusbio.com/fullsize/PABP-Antibody-10E10-Western-Blot-NB120-6125-img0003.jpg "Western Blot: PABP Antibody (10E10) [NB120-6125] - Analysis of PABP expression in 1) A-431, 2) HeLa and 3) NIH-3T3 whole cell lysates.")
![Immunocytochemistry/Immunofluorescence: PABP Antibody (10E10) [NB120-6125] - PABP antibody was tested in HeLa cells at a 1:50 dilution using a Dylight 488 conjugated secondary antibody (Green). Actin (Red) and DNA (Blue) were counterstained using Phalloidin 568 and DAPI.](http://images.novusbio.com/fullsize/PABP-Antibody-10E10-Immunocytochemistry-Immunofluorescence-NB120-6125-img0005.jpg)
![Flow Cytometry: PABP Antibody (10E10) [NB120-6125] - An intracellular stain was performed on A431 cells with PABP [10E10] Antibody NB120-6125AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/PABP-Antibody-10E10-Flow-Cytometry-NB120-6125-img0006.jpg)
![Western Blot: PABP Antibody (10E10) [NB120-6125] - Cell line lysates were separated on SDS-PAGE and probed with 1 ug/mL Monoclonal Anti-PABP Clone: 10E10. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. (1) HEK293T, (2) HeLa, (3) G361 and (4) COS-7.](http://images.novusbio.com/fullsize/PABP-Antibody-10E10-Western-Blot-NB120-6125-img0002.jpg)
![Western Blot: PABP Antibody [NBP2-19670] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PABP antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PABP-Antibody-Western-Blot-NBP2-19670-img0012.jpg "Western Blot: PABP Antibody [NBP2-19670] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PABP antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: PABP Antibody [NBP2-19670] - H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PABP protein stained by PABP antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/PABP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19670-img0006.jpg)
![Immunohistochemistry-Paraffin: PABP Antibody [NBP2-19670] - Paraffin-embedded mouse middle brain. PABP antibody dilution: 1:500.](http://images.novusbio.com/fullsize/PABP-Antibody-Immunohistochemistry-Paraffin-NBP2-19670-img0005.jpg)
![Western Blot: PABP Antibody [NBP2-19670] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PABP antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PABP-Antibody-Western-Blot-NBP2-19670-img0009.jpg)
![Immunohistochemistry-Paraffin: PABP Antibody [NBP2-19670] - Immunohistochemical analysis of paraffin-embedded Hepatoma, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/PABP-Antibody-Immunohistochemistry-Paraffin-NBP2-19670-img0002.jpg)
![Immunohistochemistry-Paraffin: PABP Antibody [NBP2-19670] - Paraffin-embedded rat hind brain. PABP antibody dilution: 1:500.](http://images.novusbio.com/fullsize/PABP-Antibody-Immunohistochemistry-Paraffin-NBP2-19670-img0003.jpg)
![Immunohistochemistry-Paraffin: PABP Antibody [NBP2-19670] - Paraffin-embedded mouse testis. PABP antibody dilution: 1:500.](http://images.novusbio.com/fullsize/PABP-Antibody-Immunohistochemistry-Paraffin-NBP2-19670-img0004.jpg)
![Immunohistochemistry-Paraffin: PABP Antibody [NBP2-19670] - Mouse testis.PABP stained by PABP antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/antibody/nbp2-19670_rabbit-polyclonal-pabp-antibody-immunohistochemistry-paraffin-2532024111751..jpg)
![Western Blot: PABP Antibody [NBP2-19670] -Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PABP antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301)) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp2-19670_rabbit-polyclonal-pabp-antibody-western-blot-2532024112021..jpg)
![Western Blot: PABP Antibody [NBP2-93914] - Analysis of extracts of various cell lines, using PABP at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/PABP-Antibody-Western-Blot-NBP2-93914-img0006.jpg "Western Blot: PABP Antibody [NBP2-93914] - Analysis of extracts of various cell lines, using PABP at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: PABP Antibody [NBP2-93914] - Analysis of U-2 OS cells using PABP . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PABP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93914-img0002.jpg)
![Immunohistochemistry-Paraffin: PABP Antibody [NBP2-93914] - Paraffin-embedded rat testis using PABP .](http://images.novusbio.com/fullsize/PABP-Antibody-Immunohistochemistry-Paraffin-NBP2-93914-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: PABP Antibody [NBP2-93914] - Analysis of L929 cells using PABP . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PABP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93914-img0001.jpg)
![Immunohistochemistry-Paraffin: PABP Antibody [NBP2-93914] - Paraffin-embedded human esophageal using PABP .](http://images.novusbio.com/fullsize/PABP-Antibody-Immunohistochemistry-Paraffin-NBP2-93914-img0003.jpg)
![Immunohistochemistry-Paraffin: PABP Antibody [NBP2-93914] - Paraffin-embedded mouse testis using PABP .](http://images.novusbio.com/fullsize/PABP-Antibody-Immunohistochemistry-Paraffin-NBP2-93914-img0004.jpg)
![Western Blot: PABP Antibody [NBP2-57869] - Analysis using Anti-PABPC1 antibody NBP2-57869 (A) shows similar pattern to independent antibody NBP2-54722 (B).](http://images.novusbio.com/fullsize/PABP-Antibody-Western-Blot-NBP2-57869-img0003.jpg "Western Blot: PABP Antibody [NBP2-57869] - Analysis using Anti-PABPC1 antibody NBP2-57869 (A) shows similar pattern to independent antibody NBP2-54722 (B).")
![Immunocytochemistry/Immunofluorescence: PABP Antibody [NBP2-57869] - Staining of human cell line RT4 shows localization to cytosol.](http://images.novusbio.com/fullsize/PABP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57869-img0001.jpg)
![Western Blot: PABP Antibody [NBP2-57869] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/PABP-Antibody-Western-Blot-NBP2-57869-img0002.jpg)
![Western Blot: PABP Antibody [NBP2-57869] - Analysis in human cell line MOLT-4.](http://images.novusbio.com/fullsize/PABP-Antibody-Western-Blot-NBP2-57869-img0004.jpg)
![Immunohistochemistry-Paraffin: PABP Antibody [NBP2-54722] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/PABP-Antibody-Immunohistochemistry-Paraffin-NBP2-54722-img0012.jpg "Immunohistochemistry-Paraffin: PABP Antibody [NBP2-54722] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.")
![Immunohistochemistry-Paraffin: PABP Antibody [NBP2-54722] - Analysis in human testis and skeletal muscle tissues using NBP2-54722 antibody. Corresponding PABPC1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PABP-Antibody-Immunohistochemistry-Paraffin-NBP2-54722-img0008.jpg)
![Immunohistochemistry-Paraffin: PABP Antibody [NBP2-54722] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/PABP-Antibody-Immunohistochemistry-Paraffin-NBP2-54722-img0009.jpg)
![Immunohistochemistry-Paraffin: PABP Antibody [NBP2-54722] - Staining of human prostate shows no cytoplasmic positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/PABP-Antibody-Immunohistochemistry-Paraffin-NBP2-54722-img0010.jpg)
![Immunohistochemistry-Paraffin: PABP Antibody [NBP2-54722] - Staining of human skeletal muscle shows no cytoplasmic positivity in myocytes as expected.](http://images.novusbio.com/fullsize/PABP-Antibody-Immunohistochemistry-Paraffin-NBP2-54722-img0011.jpg)
![Western Blot: PABP Overexpression Lysate (Adult Normal) [NBP2-10839] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PABP.](http://images.novusbio.com/fullsize/PABP-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10839-img0001.jpg "Western Blot: PABP Overexpression Lysate (Adult Normal) [NBP2-10839] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PABP.")