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![Immunohistochemistry-Paraffin: Niemann-Pick type C2 Antibody [NBP1-84012] - Analysis in human epididymis and skeletal muscle tissues. Corresponding NPC2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Niemann-Pick-type-C2-Antibody-Immunohistochemistry-Paraffin-NBP1-84012-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Niemann-Pick type C2 Antibody [NBP1-84012] - Analysis in human epididymis and skeletal muscle tissues. Corresponding NPC2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Niemann-Pick type C2 Antibody [NBP1-84012] - Analysis in human cell lines U2OS and HeLa using Anti-NPC2 antibody. Corresponding NPC2 RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.](http://images.novusbio.com/fullsize/Niemann-Pick-type-C2-Antibody-Western-Blot-NBP1-84012-img0007.jpg)
![Western Blot: Niemann-Pick type C2 Antibody [NBP1-84012] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Niemann-Pick-type-C2-Antibody-Western-Blot-NBP1-84012-img0005.jpg)
![Immunohistochemistry-Paraffin: Niemann-Pick type C2 Antibody [NBP1-84012] - Staining of human endometrium shows low expression as expected.](http://images.novusbio.com/fullsize/Niemann-Pick-type-C2-Antibody-Immunohistochemistry-Paraffin-NBP1-84012-img0010.jpg)
![Immunohistochemistry-Paraffin: Niemann-Pick type C2 Antibody [NBP1-84012] - Staining of human epididymis shows high expression.](http://images.novusbio.com/fullsize/Niemann-Pick-type-C2-Antibody-Immunohistochemistry-Paraffin-NBP1-84012-img0011.jpg)
![Immunohistochemistry-Paraffin: Niemann-Pick type C2 Antibody [NBP1-84012] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Niemann-Pick-type-C2-Antibody-Immunohistochemistry-Paraffin-NBP1-84012-img0012.jpg)
![Immunohistochemistry-Paraffin: Niemann-Pick type C2 Antibody [NBP1-84012] - Staining of human testis shows moderate to strong granular cytoplasmic positivity in Leydig cells and cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Niemann-Pick-type-C2-Antibody-Immunohistochemistry-Paraffin-NBP1-84012-img0013.jpg)
![Western Blot: Niemann-Pick type C2 Antibody (S09-8D3) [NBP3-14872] - Western blot detection of Niemann-Pick type C2 in C6, 3T3, Hela cell lysates using Niemann-Pick type C2 Rabbit mAb (1:1000 diluted). Predicted band size: 17kDa. Observed band size: 17kDa.](http://images.novusbio.com/fullsize/Niemann-Pick-type-C2-Antibody-S09-8D3-Western-Blot-NBP3-14872-img0001.jpg "Western Blot: Niemann-Pick type C2 Antibody (S09-8D3) [NBP3-14872] - Western blot detection of Niemann-Pick type C2 in C6, 3T3, Hela cell lysates using Niemann-Pick type C2 Rabbit mAb (1:1000 diluted). Predicted band size: 17kDa. Observed band size: 17kDa.")
![Immunohistochemistry-Paraffin: Niemann-Pick type C2 Antibody (S09-8D3) [NBP3-14872] - Immunohistochemistry of Niemann-Pick type C2 in paraffin-embedded Human lung cancer tissue using Niemann-Pick type C2 Rabbit mAb at dilution 1/50](http://images.novusbio.com/fullsize/Niemann-Pick-type-C2-Antibody-S09-8D3-Immunohistochemistry-Paraffin-NBP3-14872-img0002.jpg)
![Western Blot: Niemann-Pick type C2 Antibody (4B9) [H00010577-M01] - Analysis of NPC2 expression in transfected 293T cell line by NPC2 monoclonal antibody (M01), clone 4B9.Lane 1: NPC2 transfected lysate(16.6 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Niemann-Pick-type-C2-Antibody-4B9-Western-Blot-H00010577-M01-img0002.jpg "Western Blot: Niemann-Pick type C2 Antibody (4B9) [H00010577-M01] - Analysis of NPC2 expression in transfected 293T cell line by NPC2 monoclonal antibody (M01), clone 4B9.Lane 1: NPC2 transfected lysate(16.6 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: Niemann-Pick type C2 Antibody [NBP1-52028] - Western blot analysis of Niemann-Pick type C2 in Human Testis lysate (35ug protein in RIPA buffer) using NBP1-52028 at 0.5ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Niemann-Pick-type-C2-Antibody-Western-Blot-NBP1-52028-img0003.jpg "Western Blot: Niemann-Pick type C2 Antibody [NBP1-52028] - Western blot analysis of Niemann-Pick type C2 in Human Testis lysate (35ug protein in RIPA buffer) using NBP1-52028 at 0.5ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: Niemann-Pick type C2 Antibody [NBP2-15044] - Sample (30 ug of whole cell lysate) A: HeLa 15% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Niemann-Pick-type-C2-Antibody-Western-Blot-NBP2-15044-img0004.jpg "Western Blot: Niemann-Pick type C2 Antibody [NBP2-15044] - Sample (30 ug of whole cell lysate) A: HeLa 15% SDS PAGE; antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: Niemann-Pick type C2 Antibody [NBP2-15044] - Protein at cytoplasm on lung carcinoma dilution: 1:250.](http://images.novusbio.com/fullsize/Niemann-Pick-type-C2-Antibody-Immunohistochemistry-Paraffin-NBP2-15044-img0003.jpg)
![Western Blot: Niemann-Pick type C2 Antibody (012) [NBP2-90209] - Anti-NPC2 rabbit monoclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: NIH-3T3 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique.](http://images.novusbio.com/fullsize/Niemann-Pick-type-C2-Antibody-012-Western-Blot-NBP2-90209-img0004.jpg "Western Blot: Niemann-Pick type C2 Antibody (012) [NBP2-90209] - Anti-NPC2 rabbit monoclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: NIH-3T3 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique.")
![Immunoprecipitation: Niemann-Pick type C2 Antibody (012) [NBP2-90209] - NPC2-His was immunoprecipitated using: Lane A:0.5 mg NIH-3T3 Whole Cell Lysate. 2 uL anti-NPC2-His rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-NPC2-His rabbit monoclonal antibody,at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique.](http://images.novusbio.com/fullsize/Niemann-Pick-type-C2-Antibody-012-Immunoprecipitation-NBP2-90209-img0003.jpg)
![Sandwich ELISA: Niemann-Pick type C2 Antibody Pair [HRP] [NBP2-79413] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.](http://images.novusbio.com/fullsize/Niemann-Pick-type-C2-Antibody-Pair-[HRP]-Sandwich-ELISA-NBP2-79413-img0001.jpg "Sandwich ELISA: Niemann-Pick type C2 Antibody Pair [HRP] [NBP2-79413] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.")
![Immunohistochemistry-Paraffin: Niemann-Pick type C2 Antibody (029) [NBP2-90210] - Immunochemical staining of human NPC2 in human epididymis with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Niemann-Pick-type-C2-Antibody-029-Immunohistochemistry-Paraffin-NBP2-90210-img0001.jpg "Immunohistochemistry-Paraffin: Niemann-Pick type C2 Antibody (029) [NBP2-90210] - Immunochemical staining of human NPC2 in human epididymis with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).")
![ELISA: Human Niemann-Pick type C2 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39167] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39167_human-niemann-pick-type-c2-ready-to-use-elisa-kit-color-2110202415362332.png "ELISA: Human Niemann-Pick type C2 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39167] - Standard Curve Reference")
![ELISA: Human Niemann-Pick type C2 ELISA Kit (Colorimetric) [NBP3-39166] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39166_human-niemann-pick-type-c2-elisa-kit-colorimetric-2110202415273874.png "ELISA: Human Niemann-Pick type C2 ELISA Kit (Colorimetric) [NBP3-39166] - Standard Curve Reference")