| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33062 | |||
| IHC-Prediluted | NBP2-48235 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47969-0.1mg | |||
| IHC-Prediluted | NBP2-48292 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44725 | |||
| Azide and BSA Free | NBP2-34427-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08552 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48293 | |||
| Azide and BSA Free | NBP3-11576 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72977 | |||
![Knockout Validated: NF-M Antibody [NB300-133] - Analysis lysates of HeLa human cervical epithelial carcinoma parental cell line and NF-M knockout (KO) HeLa cell line. PVDF membrane was probed with 1:5,000 of Rabbit Anti-Human NF-M Polyclonal Antibody (Catalog # NB300-133) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for NF-M at approximately 155 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/NF-M-Antibody-Knockout-Validated-NB300-133-img0010.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: NF-M Antibody [NB300-133] - Analysis lysates of HeLa human cervical epithelial carcinoma parental cell line and NF-M knockout (KO) HeLa cell line. PVDF membrane was probed with 1:5,000 of Rabbit Anti-Human NF-M Polyclonal Antibody (Catalog # NB300-133) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for NF-M at approximately 155 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: NF-M Antibody [NB300-133] - Analysis of rat cerebellum section stained with rabbit pAb to NF-M, NB300-133, dilution 1:2,000 in red, and costained with mouse mAb to GAP43, dilution 1:2,000 in green. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with the above antibodies. NB300-133 antibody strongly labels neuronal processes throughout the cerebellum, while the GAP43 antibody stains predominantly synaptic regios in the molecular layer.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunocytochemistry-Immunofluorescence-NB300-133-img0008.jpg)
![Western Blot: NF-M Antibody [NB300-133] - Analysis of neuronal tissue lysates using rabbit pAb to NF-M, NB300-133, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] pig brain, [7] pig spinal cord. Strong bands at 145kDa correspond to rodent NF-M molecules, while the NF-M of pig and other larger mammals including humans run at about 160kDa.](http://images.novusbio.com/fullsize/NF-M-Antibody-Western-Blot-NB300-133-img0009.jpg)
![Immunohistochemistry-Frozen: NF-M Antibody [NB300-133] - Staining of cerebral cortex section from Rat with NF-M antibody (red) at 1:5000 dilution. This immunostaining reveals the perikarya of pyramidal neurons and dendrites as well as axons surrounding the neurons. The green channel shows staining with a monoclonal antibody to beta-adrendergic receptor kinase 1.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunohistochemistry-Frozen-NB300-133-img0007.jpg)
![Western Blot: NF-M Antibody [NB300-133] - Analysis of 160kDa Neurofilament Medium expression on whole rat cerebellum homogenate using NB300-133.](http://images.novusbio.com/fullsize/p_165_1.jpg)
![Immunocytochemistry/Immunofluorescence: NF-M Antibody [NB300-133] - Mixed neuron/glia cultures stained with NB300-133 (red) and NBP2-25141 (green), Novus' mouse monoclonal antibody to Fox1, an mRNA binding protein closely related to Fox3/NeuN. The RPCA-NF-M antibody stains axonal, dendritic and perikaryal profiles of neurons cleanly and specifically. Like antibody to Fox3/NeuN, the Fox1 antibody binds to the nuclei of neurons only. DNA is shown in blue with the DAPI stain.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunocytochemistry-Immunofluorescence-NB300-133-img0005.jpg)
![Western Blot: NF-M Antibody (MCA-3H11) [NBP2-31206] - Analysis of neuronal tissue lysates using mouse NF-M mAb, dilution 1:10,000 (Green): [1] protein standard (red), [2] rat spinal cord, [3] mouse spinal cord, [4] cow spinal cord, [5] rat sciatic nerve. Strong bands at 145kDa correspond to rodent NF-M while that at about 160kDa corresponds to the significantly larger bovine NF-M protein.](http://images.novusbio.com/fullsize/NF-M-Antibody-MCA-3H11-Western-Blot-NBP2-31206-img0003.jpg "Western Blot: NF-M Antibody (MCA-3H11) [NBP2-31206] - Analysis of neuronal tissue lysates using mouse NF-M mAb, dilution 1:10,000 (Green): [1] protein standard (red), [2] rat spinal cord, [3] mouse spinal cord, [4] cow spinal cord, [5] rat sciatic nerve. Strong bands at 145kDa correspond to rodent NF-M while that at about 160kDa corresponds to the significantly larger bovine NF-M protein.")
![Immunohistochemistry Free-Floating: NF-M Antibody (MCA-3H11) [NBP2-31206] - Analysis of adult rat frontal cortex section stained with mouse neurofilament NF-M mAb, dilution 1:5,000 (Green), and costained with chicken neurofilament NF-H pAb, dilution 1:5,000 (Red). Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24hrs, cut to 45uM, and free-floating sections were stained with above antibodies. The mouse NF-M mAb labels neuron cell bodies and dendrites of pyramidal neurons, as well as dendrites and axons of other neuronal cells, while the chicken NF-M pAb stains the network of neuronal axons only.](http://images.novusbio.com/fullsize/NF-M-Antibody-MCA-3H11-Immunohistochemistry-Free-Floating-NBP2-31206-img0004.jpg)
![Western Blot: Neurofilament NF-M Antibody (MCA-3H11) [NBP2-31206] - Cytoskeletal homogenate of rat spinal cord was separated by 7.5% SDS-PAGE. Lane A: Western blot analysis of Neurofilament NF-M with NBP2-31206. Lane B: SDS-PAGE gel stained with Commassie Brilliant Blue. The bands corresponding to Neurofilament subunits NF-H, NF-M, NF-L, a-internexin and GFAP are indicated.](http://images.novusbio.com/fullsize/Neurofilament-NF-M-Antibody-MCA-3H11-Western-Blot-NBP2-31206-img0002.jpg)
![Western Blot: NF-H Antibody (NAP4) [NB300-136] - Analysis of tissue lysates using mouse mAb to NF-H, NB300-136, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] pig spinal cord, [7] cow spinal cord. Strong band at about 200-220 kDa corresponds to the major phosphorylated from of the NF-H subunit. A minor band at about 160 kDa is the non-phosphorylated NF-H. Smaller proteolytic fragments of NF-H are also detected in spinal cord preparations with NB300-136 antibody.](http://images.novusbio.com/fullsize/NF-H-Antibody-NAP4-Western-Blot-NB300-136-img0002.jpg "Western Blot: NF-H Antibody (NAP4) [NB300-136] - Analysis of tissue lysates using mouse mAb to NF-H, NB300-136, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] pig spinal cord, [7] cow spinal cord. Strong band at about 200-220 kDa corresponds to the major phosphorylated from of the NF-H subunit. A minor band at about 160 kDa is the non-phosphorylated NF-H. Smaller proteolytic fragments of NF-H are also detected in spinal cord preparations with NB300-136 antibody.")
![Immunohistochemistry: NF-H Antibody (NAP4) [NB300-136] - Analysis of human cerebellar cortex section stained with mouse mAb to pNF-H, NB300-136, in brown. Paraffin-embedded, formalin-fixed tissue sections were stained with this antibody using the avidin biotin conjugate method. The sections was counterstained with Hematoxylin in blue. NB300-136 stains prominent basket cell axons surrounding the large Purkinje neurons. Cerebellar granule cell layer is at the bottom of the image, the molecular layer at the top.](http://images.novusbio.com/fullsize/NF-H-Antibody-NAP4-Immunohistochemistry-NB300-136-img0001.jpg)
![Western Blot: NF-H Antibody (RT97) [NBP2-29435] - Western Blot Analysis of human brain tissue lysate using NF-H Antibody (RT97).](http://images.novusbio.com/fullsize/NF-H-Antibody-RT97-Western-Blot-NBP2-29435-img0008.jpg "Western Blot: NF-H Antibody (RT97) [NBP2-29435] - Western Blot Analysis of human brain tissue lysate using NF-H Antibody (RT97).")
![Immunohistochemistry-Paraffin: NF-H Antibody (RT97) [NBP2-29435] - Formalin-fixed, paraffin-embedded Rat Cerebellum stained with NF-H Antibody (RT97).](http://images.novusbio.com/fullsize/NF-H-Antibody-RT97-Immunohistochemistry-Paraffin-NBP2-29435-img0007.jpg)
![Immunohistochemistry-Paraffin: NF-H Antibody (RT97) [NBP2-29435] - Formalin-fixed, paraffin-embedded human Cerebellum stained with NF-H Antibody (RT97).](http://images.novusbio.com/fullsize/NF-H-Antibody-RT97-Immunohistochemistry-Paraffin-NBP2-29435-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: NF-L Antibody (NR-4) [NBP2-44891] - Immunofluorescence staining of HEK293 cells using NF-L Antibody (NR-4) followed by goat anti-Mouse IgG conjugated to CF488 (green). Membrane stained with Phalloidin (Red).](http://images.novusbio.com/fullsize/NF-L-Antibody-NR-4-Immunocytochemistry-Immunofluorescence-NBP2-44891-img0005.jpg "Immunocytochemistry/Immunofluorescence: NF-L Antibody (NR-4) [NBP2-44891] - Immunofluorescence staining of HEK293 cells using NF-L Antibody (NR-4) followed by goat anti-Mouse IgG conjugated to CF488 (green). Membrane stained with Phalloidin (Red).")
![Immunohistochemistry-Paraffin: NF-L Antibody (NR-4) [NBP2-44891] - Rat Cerebellum stained with Neurofilament Monoclonal Antibody (NR-4).](http://images.novusbio.com/fullsize/NF-L-Antibody-NR-4-Immunohistochemistry-Paraffin-NBP2-44891-img0002.jpg)
![Flow Cytometry: NF-L Antibody (NR-4) [NBP2-44891] - Flow Cytometric Analysis of HEK293 cells using NF-L Antibody (NR-4) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).](http://images.novusbio.com/fullsize/NF-L-Antibody-NR-4-Flow-Cytometry-NBP2-44891-img0004.jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody (NR-4) [NBP2-44891] - Human Cerebellum stained with Neurofilament Monoclonal Antibody (NR-4).](http://images.novusbio.com/fullsize/NF-L-Antibody-NR-4-Immunohistochemistry-Paraffin-NBP2-44891-img0001.jpg)
![Western Blot: NF-H Antibody (SPM563) [NBP2-34380] - Western Blot Analysis of human brain tissue lysate using NF-H MAb (SPM563).](http://images.novusbio.com/fullsize/NF-H-Antibody-SPM563-Western-Blot-NBP2-34380-img0002.jpg "Western Blot: NF-H Antibody (SPM563) [NBP2-34380] - Western Blot Analysis of human brain tissue lysate using NF-H MAb (SPM563).")
![Immunohistochemistry-Paraffin: NF-H Antibody (SPM563) [NBP2-34380] - Formalin-fixed, paraffin-embedded human cerebellum stained with Neurofilament Monoclonal Antibody (SPM563)](http://images.novusbio.com/fullsize/NF-H-Antibody-SPM563-Immunohistochemistry-Paraffin-NBP2-34380-img0001.jpg)
![Western Blot: NF-H Antibody (NEFL.H/2324R) [NBP3-07406] - Western Blot Analysis of human brain lysate using NF-H Rabbit Recombinant Monoclonal Ab (NEFL.H/2324R).](http://images.novusbio.com/fullsize/NF-H-Antibody-NEFL-H-2324R-Western-Blot-NBP3-07406-img0003.jpg "Western Blot: NF-H Antibody (NEFL.H/2324R) [NBP3-07406] - Western Blot Analysis of human brain lysate using NF-H Rabbit Recombinant Monoclonal Ab (NEFL.H/2324R).")
![Immunohistochemistry-Paraffin: NF-H Antibody (NEFL.H/2324R) [NBP3-07406] - Formalin-fixed, paraffin-embedded human Cerebellum stained with NF-H Rabbit Recombinant Monoclonal Antibody (NEFL.H/2324R).](http://images.novusbio.com/fullsize/NF-H-Antibody-NEFL-H-2324R-Immunohistochemistry-Paraffin-NBP3-07406-img0002.jpg)
![Flow Cytometry: NF-H Antibody (NEFL.H/2324R) [NBP3-07406] - Flow Cytometric Analysis of HEK293 cells using NF-H Rabbit Recombinant Monoclonal Ab (NEFL.H/2324R) followed by goat anti- Mouse IgG-CF488 (Blue); Isotype control (Red).](http://images.novusbio.com/fullsize/NF-H-Antibody-NEFL-H-2324R-Flow-Cytometry-NBP3-07406-img0001.jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody (SPM204) [NBP2-44892] - Cerebellum stained with Neurofilament Monoclonal Antibody (SPM204).](http://images.novusbio.com/fullsize/NF-L-Antibody-SPM204-Immunohistochemistry-Paraffin-NBP2-44892-img0001.jpg "Immunohistochemistry-Paraffin: NF-L Antibody (SPM204) [NBP2-44892] - Cerebellum stained with Neurofilament Monoclonal Antibody (SPM204).")
![Western Blot: 68kDa Neurofilament Light Overexpression Lysate (Adult Normal) [NBL1-13577] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for 68kDa Neurofilament Light.](http://images.novusbio.com/fullsize/68kDa-Neurofilament-Light-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13577-img0002.jpg "Western Blot: 68kDa Neurofilament Light Overexpression Lysate (Adult Normal) [NBL1-13577] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for 68kDa Neurofilament Light.")
![Western Blot: 160kDa Neurofilament Medium Overexpression Lysate (Adult Normal) [NBL1-13578] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for 160kDa Neurofilament Medium.](http://images.novusbio.com/fullsize/160kDa-Neurofilament-Medium-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13578-img0002.jpg "Western Blot: 160kDa Neurofilament Medium Overexpression Lysate (Adult Normal) [NBL1-13578] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for 160kDa Neurofilament Medium.")