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![Western Blot: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Western blot analysis of Myosin light chain kinase on human lung tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperatur](http://images.novusbio.com/fullsize/Myosin-light-chain-kinase-Antibody-SU40-06-Western-Blot-NBP2-67486-img0010.jpg "Western Blot: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Western blot analysis of Myosin light chain kinase on human lung tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperatur")
![Immunocytochemistry/Immunofluorescence: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Staining MYLK in L6 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Myosin-light-chain-kinase-Antibody-SU40-06-Immunocytochemistry-Immunofluorescence-NBP2-67486-img0009.jpg)
![Immunohistochemistry-Paraffin: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Analysis of paraffin-embedded rat smooth muscle tissue using anti-MYLK antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Myosin-light-chain-kinase-Antibody-SU40-06-Immunohistochemistry-Paraffin-NBP2-67486-img0007.jpg)
![Flow Cytometry: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Analysis of SH-SY-5Y cells with MYLK antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Myosin-light-chain-kinase-Antibody-SU40-06-Flow-Cytometry-NBP2-67486-img0001.jpg)
![Western Blot: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Analysis of MYLK on human lung lysates using anti-MYLK antibody at 1/5,000 dilution.](http://images.novusbio.com/fullsize/Myosin-light-chain-kinase-Antibody-SU40-06-Western-Blot-NBP2-67486-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Staining MYLK in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Myosin-light-chain-kinase-Antibody-SU40-06-Immunocytochemistry-Immunofluorescence-NBP2-67486-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Staining MYLK in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Myosin-light-chain-kinase-Antibody-SU40-06-Immunocytochemistry-Immunofluorescence-NBP2-67486-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Staining MYLK in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Myosin-light-chain-kinase-Antibody-SU40-06-Immunocytochemistry-Immunofluorescence-NBP2-67486-img0004.jpg)
![Immunohistochemistry-Paraffin: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Analysis of paraffin-embedded human colon cancer tissue using anti-MYLK antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Myosin-light-chain-kinase-Antibody-SU40-06-Immunohistochemistry-Paraffin-NBP2-67486-img0005.jpg)
![Immunohistochemistry-Paraffin: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Analysis of paraffin-embedded mouse smooth muscle tissue using anti-MYLK antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Myosin-light-chain-kinase-Antibody-SU40-06-Immunohistochemistry-Paraffin-NBP2-67486-img0006.jpg)
![Immunohistochemistry-Paraffin: Myosin light chain kinase Antibody [NBP1-87744] - Staining in human smooth muscle and skeletal muscle tissues using anti-MYLK antibody. Corresponding MYLK RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Myosin-light-chain-kinase-Antibody-Immunohistochemistry-Paraffin-NBP1-87744-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Myosin light chain kinase Antibody [NBP1-87744] - Staining in human smooth muscle and skeletal muscle tissues using anti-MYLK antibody. Corresponding MYLK RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Myosin light chain kinase Antibody [NBP1-87744] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Human cell line HEL](http://images.novusbio.com/fullsize/Myosin-light-chain-kinase-Antibody-Western-Blot-NBP1-87744-img0008.jpg)
![Immunohistochemistry-Paraffin: Myosin light chain kinase Antibody [NBP1-87744] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/Myosin-light-chain-kinase-Antibody-Immunohistochemistry-Paraffin-NBP1-87744-img0009.jpg)
![Immunohistochemistry-Paraffin: Myosin light chain kinase Antibody [NBP1-87744] - Staining of human smooth muscle shows high expression.](http://images.novusbio.com/fullsize/Myosin-light-chain-kinase-Antibody-Immunohistochemistry-Paraffin-NBP1-87744-img0010.jpg)
![Western Blot: Myosin light chain kinase Antibody [H00004638-D01P] - Analysis of MYLK expression in human stomach.](http://images.novusbio.com/fullsize/Myosin-light-chain-kinase-Antibody-Western-Blot-H00004638-D01P-img0004.jpg "Western Blot: Myosin light chain kinase Antibody [H00004638-D01P] - Analysis of MYLK expression in human stomach.")
![Western Blot: Myosin light chain kinase Antibody [H00004638-D01P] - Analysis of MYLK expression in transfected 293T cell line by MYLK polyclonal antibody.Lane 1: MYLK transfected lysate(110.20 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Myosin-light-chain-kinase-Antibody-Western-Blot-H00004638-D01P-img0005.jpg)
![Proximity Ligation Assay: Myosin light chain kinase Antibody [H00004638-D01P] - Analysis of protein-protein interactions between MYLK and CTTN. HeLa cells were stained with anti-MYLK rabbit purified polyclonal 1:1200 and anti-CTTN mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Myosin-light-chain-kinase-Antibody-Proximity-Ligation-Assay-H00004638-D01P-img0003.jpg)
![Western Blot: Myosin light chain kinase Antibody (28C5) [NBP3-26122] - Positive Western Blot detected in: HepG2 whole cell lysate, HEK293 whole cell lysate. All lanes: Myosin light chain kinase Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 211, 204, 206, 198, 203, 111, 17, 81 kDa Observed band size: 130-250 kDa](http://images.novusbio.com/fullsize/nbp3-26122_rabbit-myosin-light-chain-kinase-mab-28c5-282202411472135.jpg "Western Blot: Myosin light chain kinase Antibody (28C5) [NBP3-26122] - Positive Western Blot detected in: HepG2 whole cell lysate, HEK293 whole cell lysate. All lanes: Myosin light chain kinase Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 211, 204, 206, 198, 203, 111, 17, 81 kDa Observed band size: 130-250 kDa")
![Flow Cytometry: Myosin light chain kinase Antibody (28C5) [NBP3-26122] - Overlay Peak curve showing A549 cells stained with Myosin light chain kinase Antibody (28C5) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26122_rabbit-myosin-light-chain-kinase-mab-28c5-282202414183659.jpg)
![SDS-Page: Recombinant Human Myosin light chain kinase Protein [H00004638-P02] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-Myosin-light-chain-kinase-Protein-SDS-Page-H00004638-P02-img0001.jpg "SDS-Page: Recombinant Human Myosin light chain kinase Protein [H00004638-P02] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: Myosin light chain kinase Antibody (1J2U2) [NBP3-16282] - Western blot analysis of extracts of various cell lines, using Myosin light chain kinase Rabbit mAb (NBP3-16282) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Myosin-light-chain-kinase-Antibody-1J2U2-Western-Blot-NBP3-16282-img0001.jpg "Western Blot: Myosin light chain kinase Antibody (1J2U2) [NBP3-16282] - Western blot analysis of extracts of various cell lines, using Myosin light chain kinase Rabbit mAb (NBP3-16282) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: Myosin light chain kinase Antibody [NBP2-68679] - Staining of human cell line BJ shows localization to plasma membrane & actin filaments.](http://images.novusbio.com/fullsize/Myosin-light-chain-kinase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-68679-img0001.jpg "Immunocytochemistry/Immunofluorescence: Myosin light chain kinase Antibody [NBP2-68679] - Staining of human cell line BJ shows localization to plasma membrane & actin filaments.")
![Western Blot: Myosin light chain kinase Overexpression Lysate (Adult Normal) [NBP2-05825] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Myosin light chain kinase.](http://images.novusbio.com/fullsize/Myosin-light-chain-kinase-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-05825-img0001.jpg "Western Blot: Myosin light chain kinase Overexpression Lysate (Adult Normal) [NBP2-05825] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Myosin light chain kinase.")
![Western Blot: Myosin light chain kinase Antibody (1D1) [H00004638-M01] - Analysis of MYLK expression in transfected 293T cell line by MYLK monoclonal antibody (M01), clone 1D1.Lane 1: MYLK transfected lysate(110.2 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Myosin-light-chain-kinase-Antibody-1D1-Western-Blot-H00004638-M01-img0001.jpg "Western Blot: Myosin light chain kinase Antibody (1D1) [H00004638-M01] - Analysis of MYLK expression in transfected 293T cell line by MYLK monoclonal antibody (M01), clone 1D1.Lane 1: MYLK transfected lysate(110.2 KDa).Lane 2: Non-transfected lysate.")
![ELISA: Human Myosin light chain kinase - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39555] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39555_human-myosin-light-chain-kinase-ready-to-use-elisa-kit-211020241538096.png "ELISA: Human Myosin light chain kinase - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39555] - Standard Curve Reference")
![ELISA: Human Myosin light chain kinase ELISA Kit (Colorimetric) [NBP3-39561] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39561_human-myosin-light-chain-kinase-elisa-kit-colorimetric-21102024152915.png "ELISA: Human Myosin light chain kinase ELISA Kit (Colorimetric) [NBP3-39561] - Standard Curve Reference")