| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5778-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37566-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33954-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33953-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72599 | |||
![Western Blot: MCM2 Antibody [NB100-288] - Detection of human and mouse MCM2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM2 antibody NB100-288 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/MCM2-Antibody-Western-Blot-NB100-288-img0018.jpg "Western Blot: MCM2 Antibody [NB100-288] - Detection of human and mouse MCM2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM2 antibody NB100-288 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunohistochemistry-Paraffin: MCM2 Antibody [NB100-288] - Sample: FFPE section of human small cell lung cancer. Antibody: Affinity purified rabbit anti-MCM2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunohistochemistry-NB100-288-img0017.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: MCM2 Antibody [NB100-288] - Detection of human MCM2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MCM2 antibody NB100-288 used for IP at 3 ug per reaction. MCM2 was also immunoprecipitated by rabbit anti-Phospho MCM2 (S108) antibody NB100-639. For blotting immunoprecipitated MCM2, NB100-288 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunoprecipitation-NB100-288-img0019.jpg)
![Western Blot: MCM2 Antibody (6A8) [H00004171-M01] - Analysis of MCM2 expression in transfected 293T cell line by MCM2 monoclonal antibody (M01), clone 6A8.Lane 1: MCM2 transfected lysate(101.9 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/MCM2-Antibody-6A8-Western-Blot-H00004171-M01-img0011.jpg "Western Blot: MCM2 Antibody (6A8) [H00004171-M01] - Analysis of MCM2 expression in transfected 293T cell line by MCM2 monoclonal antibody (M01), clone 6A8.Lane 1: MCM2 transfected lysate(101.9 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: MCM2 Antibody (6A8) [H00004171-M01] - Analysis of monoclonal antibody to MCM2 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/MCM2-Antibody-6A8-Immunocytochemistry-Immunofluorescence-H00004171-M01-img0007.jpg)
![Western Blot: MCM2 Antibody (6A8) [H00004171-M01] - MCM2 monoclonal antibody (M01), clone 6A8 Analysis of MCM2 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/MCM2-Antibody-6A8-Western-Blot-H00004171-M01-img0010.jpg)
![Immunoprecipitation: MCM2 Antibody (6A8) [H00004171-M01] - Analysis of MCM2 transfected lysate using anti-MCM2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MCM2 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/MCM2-Antibody-6A8-Immunoprecipitation-H00004171-M01-img0008.jpg)
![ELISA: MCM2 Antibody (6A8) [H00004171-M01] - Detection limit for recombinant GST tagged MCM2 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MCM2-Antibody-6A8-ELISA-H00004171-M01-img0012.jpg)
![Western Blot: MCM2 [p Ser108] Antibody [NB100-639] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells either mock treated (-) or treated (+) with EPE (100 uM, 2h). Antibodies: MCM2 was immunoprecipitated using rabbit anti-MCM2 antibody was used for WB at 1 mcg/ml. To examine total MCM2, the blot was stripped and then blotted with rabbit anti-MCM2 antibody . Detection: Chemiluminescence with exposure times of 10 seconds (upper and lower panels).](http://images.novusbio.com/fullsize/MCM2-[p-Ser108]-Antibody-Western-Blot-NB100-639-img0007.jpg "Western Blot: MCM2 [p Ser108] Antibody [NB100-639] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells either mock treated (-) or treated (+) with EPE (100 uM, 2h). Antibodies: MCM2 was immunoprecipitated using rabbit anti-MCM2 antibody was used for WB at 1 mcg/ml. To examine total MCM2, the blot was stripped and then blotted with rabbit anti-MCM2 antibody . Detection: Chemiluminescence with exposure times of 10 seconds (upper and lower panels).")
![Immunohistochemistry-Paraffin: MCM2 [p Ser108] Antibody [NB100-639] - Human breast carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti- phospho MCM2 (S108) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MCM2-[p-Ser108]-Antibody-Immunohistochemistry-Paraffin-NB100-639-img0008.jpg)
![Western Blot: MCM2 [p Ser108] Antibody [NB100-639] - Detection of Human phospho MCM2 (Ser108) by Western Blot. Samples: Whole cell lysate from HeLa cells that were untreated or exposed to 8 Gy IR for 1 h or 50 J/m2 for 2 hours. Antibody: Affinity purified rabbit anti-phospho MCM2 (Ser108) antibody (Cat. No. NB100-639) used at 0.2 mcg/ml. Subsequently, total MCM2 was detected to verify loading. Detection: Chemiluminescence with an exposure time of 5 seconds.](http://images.novusbio.com/fullsize/MCM2-[p-Ser108]-Antibody-Western-Blot-NB100-639-img0001.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: MCM2 [p Ser108] Antibody [NB100-639] - Whole cell lysate (15 or 50 ug) from 293T cells or mouse NIH3T3 (M) cells. Cells were either mock treated (-) or treated (+) with EPE (100 uM, 2h). Antibody used at 0.1 mcg/ml.](http://images.novusbio.com/fullsize/MCM2-[p-Ser108]-Antibody-Western-Blot-NB100-639-img0003.jpg)
![Western Blot: MCM2 Antibody (0Q10S9) [NBP3-16066] - Western blot analysis of extracts of various cell lines, using MCM2 antibody (NBP3-16066) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0.3s.](http://images.novusbio.com/fullsize/MCM2-Antibody-0Q10S9-Western-Blot-NBP3-16066-img0009.jpg "Western Blot: MCM2 Antibody (0Q10S9) [NBP3-16066] - Western blot analysis of extracts of various cell lines, using MCM2 antibody (NBP3-16066) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0.3s.")
![Immunocytochemistry/Immunofluorescence: MCM2 Antibody (0Q10S9) [NBP3-16066] - Immunofluorescence analysis of U2OS cells using MCM2 Rabbit mAb (NBP3-16066) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/MCM2-Antibody-0Q10S9-Immunocytochemistry-Immunofluorescence-NBP3-16066-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: MCM2 Antibody (0Q10S9) [NBP3-16066] - Immunofluorescence analysis of NIH/3T3 cells using MCM2 Rabbit mAb (NBP3-16066) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/MCM2-Antibody-0Q10S9-Immunocytochemistry-Immunofluorescence-NBP3-16066-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: MCM2 Antibody (0Q10S9) [NBP3-16066] - Immunofluorescence analysis of PC-12 cells using MCM2 Rabbit mAb (NBP3-16066) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/MCM2-Antibody-0Q10S9-Immunocytochemistry-Immunofluorescence-NBP3-16066-img0007.jpg)
![Immunohistochemistry: MCM2 Antibody (0Q10S9) [NBP3-16066] - Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using MCM2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16066_rabbit-mcm2-mab-0q10s9-52202511392292.jpg)
![Immunohistochemistry: MCM2 Antibody (0Q10S9) [NBP3-16066] - Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using MCM2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16066_rabbit-mcm2-mab-0q10s9-5220251215436.jpg)
![Immunohistochemistry: MCM2 Antibody (0Q10S9) [NBP3-16066] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using MCM2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16066_rabbit-mcm2-mab-0q10s9-5220251255592.jpg)
![Immunohistochemistry: MCM2 Antibody (0Q10S9) [NBP3-16066] - Immunohistochemistry analysis of paraffin-embedded Human stomach tissue using MCM2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16066_rabbit-mcm2-mab-0q10s9-52202511361222.jpg)
![Immunohistochemistry: MCM2 Antibody (0Q10S9) [NBP3-16066] - Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using MCM2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16066_rabbit-mcm2-mab-0q10s9-52202511365185.jpg)
![Immunohistochemistry: MCM2 Antibody (0Q10S9) [NBP3-16066] - Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using MCM2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16066_rabbit-mcm2-mab-0q10s9-52202511402713.jpg)
![Immunohistochemistry: MCM2 Antibody (0Q10S9) [NBP3-16066] - Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using MCM2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16066_rabbit-mcm2-mab-0q10s9-5220251242549.jpg)
![Immunohistochemistry: MCM2 Antibody (0Q10S9) [NBP3-16066] - Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using MCM2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16066_rabbit-mcm2-mab-0q10s9-522025125420.jpg)
![Immunohistochemistry: MCM2 Antibody (0Q10S9) [NBP3-16066] - Immunohistochemistry analysis of paraffin-embedded Human thyroid tissue using MCM2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16066_rabbit-mcm2-mab-0q10s9-52202511361214.jpg)
![Immunohistochemistry: MCM2 Antibody (0Q10S9) [NBP3-16066] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using MCM2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16066_rabbit-mcm2-mab-0q10s9-522025126530.jpg)
![Immunohistochemistry: MCM2 Antibody (0Q10S9) [MCM2] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using MCM2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16066_rabbit-mcm2-mab-0q10s9-52202517251914.jpg)
![Western Blot: MCM2 [p Ser53] Antibody [NB100-2339] - Detection of Human Phospho MCM2 (S53) by Western Blot. Samples: Whole cell lysate (5 and 15 ug for WB; 1 mg for IP, 20% of IP loaded) from asynchronous 293T cells. For IP/WB, MCM2 was immunoprecipitated using A300-191A, which recognizes total MCM2. The immunoprecipitate was mock treated (-) or treated (+) with phosphatase (PPase). Antibody: Affinity purified rabbit anti-phospho MCM2 (S53) antibody NB100-2339 used at 0.1 ug/ml. To examine total MCM2, the membrane was stripped and blotted with A300-191A at 0.1 ug/ml. For blotting immunoprecipitated MCM2, the ReliaBLOT Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).](http://images.novusbio.com/fullsize/MCM2-[p-Ser53]-Antibody-Western-Blot-NB100-2339-img0029.jpg "Western Blot: MCM2 [p Ser53] Antibody [NB100-2339] - Detection of Human Phospho MCM2 (S53) by Western Blot. Samples: Whole cell lysate (5 and 15 ug for WB; 1 mg for IP, 20% of IP loaded) from asynchronous 293T cells. For IP/WB, MCM2 was immunoprecipitated using A300-191A, which recognizes total MCM2. The immunoprecipitate was mock treated (-) or treated (+) with phosphatase (PPase). Antibody: Affinity purified rabbit anti-phospho MCM2 (S53) antibody NB100-2339 used at 0.1 ug/ml. To examine total MCM2, the membrane was stripped and blotted with A300-191A at 0.1 ug/ml. For blotting immunoprecipitated MCM2, the ReliaBLOT Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).")
![Immunohistochemistry: MCM2 [p Ser53] Antibody [NB100-2339] - Sample: FFPE sections of human breast carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-fs18 phospho MCM2 (S53) fs20 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MCM2-[p-Ser53]-Antibody-Immunohistochemistry-NB100-2339-img0028.jpg)
![Western Blot: MCM2 [p Ser53] Antibody [NB100-2339] - Whole cell lysate (50 ug) from asynchronous 293T cells that was mock treated (-) or treated (+) with phosphatases (PPase). Antibody used at 0.1 ug/ml. To examine total MCM2, the blot was stripped and then blotted with rabbit anti-MCM2 antibody at 0.1 ug/ml.](http://images.novusbio.com/fullsize/MCM2-[p-Ser53]-Antibody-Western-Blot-NB100-2339-img0003.jpg)
![Western Blot: MCM2 Antibody (2B3) [NBP2-37566] - Western blot analysis using MCM2 mouse mAb against PC-12 (1), Cos7 (2), NIH/3T3 (3), HepG2 (4), HEK293 (5), K562 (6), Jurkat (7), Hela (8) and MCF-7 (9) cell lysate.](http://images.novusbio.com/fullsize/MCM2-Antibody-2B3-Western-Blot-NBP2-37566-img0003.jpg "Western Blot: MCM2 Antibody (2B3) [NBP2-37566] - Western blot analysis using MCM2 mouse mAb against PC-12 (1), Cos7 (2), NIH/3T3 (3), HepG2 (4), HEK293 (5), K562 (6), Jurkat (7), Hela (8) and MCF-7 (9) cell lysate.")
![Immunohistochemistry: MCM2 Antibody (2B3) [NBP2-37566] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues using MCM2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/MCM2-Antibody-2B3-Immunohistochemistry-NBP2-37566-img0006.jpg)
![Flow Cytometry: MCM2 Antibody (2B3) [NBP2-37566] - Flow cytometric analysis of Hela cells using MCM2 mouse mAb (blue) and negative control (red).](http://images.novusbio.com/fullsize/MCM2-Antibody-2B3-Flow-Cytometry-NBP2-37566-img0004.jpg)
![Western Blot: MCM2 Antibody (2B3) [NBP2-37566] - Western blot analysis using MCM2 mAb against human MCM2 (AA: 16-232) recombinant protein.(Expected MW is 50.4 kDa)](http://images.novusbio.com/fullsize/MCM2-Antibody-2B3-Western-Blot-NBP2-37566-img0002.jpg)
![Immunohistochemistry: MCM2 Antibody (2B3) [NBP2-37566] - Immunohistochemical analysis of paraffin-embedded colon cancer tissues using MCM2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/MCM2-Antibody-2B3-Immunohistochemistry-NBP2-37566-img0005.jpg)
![ELISA: MCM2 Antibody (2B3) [NBP2-37566] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/MCM2-Antibody-2B3-ELISA-NBP2-37566-img0001.jpg)
![Western Blot: MCM2 Antibody (BLR108H) [NBP3-07021] - Detection of human Phospho-MCM2 by WB of immunoprecipitates from HeLa lysate. Antibodies: Rabbit anti-MCM2 and Phospho-MCM2. Lower Panel: Total MCM2.](http://images.novusbio.com/fullsize/MCM2-Antibody-BLR108H-Western-Blot-NBP3-07021-img0003.jpg "Western Blot: MCM2 Antibody (BLR108H) [NBP3-07021] - Detection of human Phospho-MCM2 by WB of immunoprecipitates from HeLa lysate. Antibodies: Rabbit anti-MCM2 and Phospho-MCM2. Lower Panel: Total MCM2.")
![Immunocytochemistry: MCM2 Antibody (BLR108H) [NBP3-07021] - Detection of human Phospho-MCM2 (S53) in FFPE OVCAR-4 cells by ICC. Antibody: Rabbit anti-MCM2 (S53) recombinant monoclonal [BLR108H]. Secondary: HRP-conjugated goat anti-rabbit IgG Substrate: DAB.](http://images.novusbio.com/fullsize/MCM2-Antibody-BLR108H-Immunocytochemistry-NBP3-07021-img0006.jpg)
![Immunohistochemistry: MCM2 Antibody (BLR108H) [NBP3-07021] - Detection of mouse Phospho-MCM2 (S53) in FFPE mouse renal cell carcinoma by IHC. Antibody: Rabbit anti-MCM2 (S53) recombinant monoclonal [BLR108H]. Secondary: HRP-conjugated goat anti-rabbit IgG Substrate: DAB.](http://images.novusbio.com/fullsize/MCM2-Antibody-BLR108H-Immunohistochemistry-NBP3-07021-img0005.jpg)
![Western Blot: MCM2 Antibody (BLR108H) [NBP3-07021] - Detection of human Phospho-MCM2 by WB of HDLM-2, HeLa, Jurkat, MCF-7, MOLT-4, Malme-3M, HEK293T, and Raji lysate. Antibody: Rabbit anti-Phospho-MCM2 recombinant monoclonal [BLR108H]. Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-Actin recombinant monoclonal.](http://images.novusbio.com/fullsize/MCM2-Antibody-BLR108H-Western-Blot-NBP3-07021-img0001.jpg)
![Western Blot: MCM2 Antibody (BLR108H) [NBP3-07021] - Detection of mouse Phospho-MCM2 (S53) by WB of BW5147.3, TCMK-1, mIMCD-3, A20, NIH 3T3, and CT26 lysate. Antibody: Rabbit anti-Phospho-MCM2 (S53) recombinant monoclonal [BLR108H]. Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-Actin recombinant monoclonal.](http://images.novusbio.com/fullsize/MCM2-Antibody-BLR108H-Western-Blot-NBP3-07021-img0002.jpg)
![Immunohistochemistry: MCM2 Antibody (BLR108H) [NBP3-07021] - Detection of human Phospho-MCM2 (S53) in FFPE lung carcinoma by IHC. Antibody: Rabbit anti-MCM2 (S53) recombinant monoclonal [BLR108H]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB](http://images.novusbio.com/fullsize/MCM2-Antibody-BLR108H-Immunohistochemistry-NBP3-07021-img0004.jpg)
![Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33954] - Staining in human lymph node and kidney tissues using anti-MCM2 antibody. Corresponding MCM2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunohistochemistry-Paraffin-NBP2-33954-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33954] - Staining in human lymph node and kidney tissues using anti-MCM2 antibody. Corresponding MCM2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: MCM2 Antibody [NBP2-33954] - Analysis in human cell line HEL.](http://images.novusbio.com/fullsize/MCM2-Antibody-Western-Blot-NBP2-33954-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: MCM2 Antibody [NBP2-33954] - Staining of human cell line MCF7 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33954-img0011.jpg)
![Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33954] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunohistochemistry-Paraffin-NBP2-33954-img0016.jpg)
![Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33954] - Staining of human lymph node shows high expression.](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunohistochemistry-Paraffin-NBP2-33954-img0009.jpg)
![Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33954] - Staining of human kidney shows no positivity in cells in tubules as expected.](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunohistochemistry-Paraffin-NBP2-33954-img0014.jpg)
![Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33954] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunohistochemistry-Paraffin-NBP2-33954-img0017.jpg)
![Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33953] - Analysis in human lymph node and liver tissues. Corresponding MCM2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunohistochemistry-Paraffin-NBP2-33953-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33953] - Analysis in human lymph node and liver tissues. Corresponding MCM2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: MCM2 Antibody [NBP2-33953] - Analysis in human cell line HEL.](http://images.novusbio.com/fullsize/MCM2-Antibody-Western-Blot-NBP2-33953-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: MCM2 Antibody [NBP2-33953] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33953-img0005.jpg)
![Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33953] - Staining of human pancreas shows low positivity in exocrine glanular cells as expected.](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunohistochemistry-Paraffin-NBP2-33953-img0014.jpg)
![Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33953] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunohistochemistry-Paraffin-NBP2-33953-img0011.jpg)
![Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33953] - Staining of human lymph node shows moderate nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunohistochemistry-Paraffin-NBP2-33953-img0012.jpg)
![Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33953] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunohistochemistry-Paraffin-NBP2-33953-img0013.jpg)
![Western Blot: MCM2 [p Ser41] Antibody [NB100-2569] - Whole cell lysate (50 ug) from asynchronous 293T cells that was mock treated (-) or treated (+) with phosphatases (PPase). Antibody used at 0.1 ug/ml. To examine total MCM2, the blot was stripped and then blotted with rabbit anti-MCM2 antibody at 0.1 ug/](http://images.novusbio.com/fullsize/MCM2-[p-Ser41]-Antibody-Western-Blot-NB100-2569-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: MCM2 [p Ser41] Antibody [NB100-2569] - Whole cell lysate (50 ug) from asynchronous 293T cells that was mock treated (-) or treated (+) with phosphatases (PPase). Antibody used at 0.1 ug/ml. To examine total MCM2, the blot was stripped and then blotted with rabbit anti-MCM2 antibody at 0.1 ug/")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: MCM2 [p Ser41] Antibody [NB100-2569] - Samples: FFPE serial sections of human colon carcinoma. Mock phosphatase treated section (left) and calf intestinal phosphatase-treated section (right). Antibody: Affinity purified rabbit anti-Phospho MCM2 (S40/S41) used at a dilution of 1:200 (1 ug/ml).](http://images.novusbio.com/fullsize/MCM2-[p-Ser41]-Antibody-Immunohistochemistry-NB100-2569-img0032.jpg)
![Flow Cytometry: MCM2 [p Ser41] Antibody [NB100-2569] - Flow cytometric analysis of phospho-MCM2 (pS40/41). Jurkat cells were fixed in 1.5% PFA, and permeabilized in 90% MeOH. 1 X 10e6 cells were stained with 0.1 mcg/ml anti-phospho- MCM2 (pS40/41) in a 150 mcl volume. DNA content was simultaneously analyzed via PI stain](http://images.novusbio.com/fullsize/MCM2-[p-Ser41]-Antibody-Flow-Cytometry-NB100-2569-img0002.jpg)
![Western Blot: MCM2 Antibody (3C12) [NBP2-45774] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCM2 .](http://images.novusbio.com/fullsize/MCM2-Antibody-3C12-Western-Blot-NBP2-45774-img0006.jpg "Western Blot: MCM2 Antibody (3C12) [NBP2-45774] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCM2 .")
![Immunohistochemistry: MCM2 Antibody (3C12) [NBP2-45774] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MCM2-Antibody-3C12-Immunohistochemistry-NBP2-45774-img0005.jpg)
![Immunohistochemistry: MCM2 Antibody (3C12) [NBP2-45774] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MCM2-Antibody-3C12-Immunohistochemistry-NBP2-45774-img0001.jpg)
![Immunohistochemistry: MCM2 Antibody (3C12) [NBP2-45774] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MCM2-Antibody-3C12-Immunohistochemistry-NBP2-45774-img0002.jpg)
![Immunohistochemistry: MCM2 Antibody (3C12) [NBP2-45774] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MCM2-Antibody-3C12-Immunohistochemistry-NBP2-45774-img0003.jpg)
![Immunohistochemistry: MCM2 Antibody (3C12) [NBP2-45774] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/MCM2-Antibody-3C12-Immunohistochemistry-NBP2-45774-img0004.jpg)