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![Western Blot: Kir2.1 Antibody (S112) [NBP2-12900] - Western Blot analysis of Monkey COS transient cell lysate showing detection of Kir2.1 Potassium Channel protein using Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody, Clone S112 (NBP2-12900). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody (NBP2-12900) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/Kir2-1-Antibody-S112-Western-Blot-NBP2-12900-img0005.jpg "Western Blot: Kir2.1 Antibody (S112) [NBP2-12900] - Western Blot analysis of Monkey COS transient cell lysate showing detection of Kir2.1 Potassium Channel protein using Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody, Clone S112 (NBP2-12900). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody (NBP2-12900) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: Kir2.1 Antibody (S112) [NBP2-12900] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Kir2.1 Monoclonal Antibody, Clone S112 (NBP2-12900). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Kir2.1 Monoclonal Antibody (NBP2-12900) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Kir2.1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/Kir2-1-Antibody-S112-Immunocytochemistry-Immunofluorescence-NBP2-12900-img0007.jpg)
![Immunohistochemistry: Kir2.1 Antibody (S112) [NBP2-12900] - Immunohistochemistry analysis using Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody, Clone S112 (NBP2-12900). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody (NBP2-12900) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/Kir2-1-Antibody-S112-Immunohistochemistry-NBP2-12900-img0006.jpg)
![Immunohistochemistry: Kir2.1 Antibody (S112) [NBP2-12900] - Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Nuclear expression in the epidermis and hair follicles.](http://images.novusbio.com/fullsize/Kir2-1-Antibody-S112-Immunohistochemistry-NBP2-12900-img0003.jpg)
![Western Blot: Kir2.1 Antibody (JE54-56) [NBP3-32505] - Western blot analysis of Kir2.1 on 293 cell lysates with Rabbit anti-Kir2.1 antibody (NBP3-32505) at 1/1,000 dilution. Predicted band size: 48 kDa Observed band size: 48 kDa Exposure time: 30s; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32505) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32505_rabbit-kir2-1-mab-je54-56-278202415295538.jpg "Western Blot: Kir2.1 Antibody (JE54-56) [NBP3-32505] - Western blot analysis of Kir2.1 on 293 cell lysates with Rabbit anti-Kir2.1 antibody (NBP3-32505) at 1/1,000 dilution. Predicted band size: 48 kDa Observed band size: 48 kDa Exposure time: 30s; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32505) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Kir2.1 Antibody (JE54-56) [NBP3-32505] - Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-Kir2.1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32505, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32505_rabbit-kir2-1-mab-je54-56-298202414333623.jpg)
![Immunocytochemistry/ Immunofluorescence: Kir2.1 Antibody (JE54-56) [NBP3-32505] - ICC staining of Kir2.1 in N2A cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (NBP3-32505, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32505_rabbit-kir2-1-mab-je54-56-29202410484869.jpg)
![Immunohistochemistry-Paraffin: Kir2.1 Antibody [NBP1-87709] - Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/Kir2.1-Antibody-Immunohistochemistry-Paraffin-NBP1-87709-img0007.jpg "Immunohistochemistry-Paraffin: Kir2.1 Antibody [NBP1-87709] - Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.")
![Immunohistochemistry: Kir2.1 Antibody [NBP1-87709] - Staining of human colon shows strong cytoplasmic and membrane positivity in glandular cells.](http://images.novusbio.com/fullsize/Kir2.1-Antibody-Immunohistochemistry-NBP1-87709-img0004.jpg)
![Immunohistochemistry-Paraffin: Kir2.1 Antibody [NBP1-87709] - Staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Kir2.1-Antibody-Immunohistochemistry-Paraffin-NBP1-87709-img0005.jpg)
![Immunohistochemistry-Paraffin: Kir2.1 Antibody [NBP1-87709] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Kir2.1-Antibody-Immunohistochemistry-Paraffin-NBP1-87709-img0006.jpg)
![Immunohistochemistry-Paraffin: Kir2.1 Antibody [NBP1-87709] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/Kir2.1-Antibody-Immunohistochemistry-Paraffin-NBP1-87709-img0008.jpg)
![Western Blot: Kir2.1 Antibody (SR1939) [NBP3-22328] - Western blot analysis using NBP3-22328 in A549 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22328_rabbit-kir2-1-mab-sr1939-57202315545127.jpg "Western Blot: Kir2.1 Antibody (SR1939) [NBP3-22328] - Western blot analysis using NBP3-22328 in A549 cell lysate.")
![Western Blot: Kir2.1 Antibody [NBP3-03675] - Analysis of extracts of various cell lines, using Kir2.1 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit](http://images.novusbio.com/fullsize/Kir2.1-Antibody-Western-Blot-NBP3-03675-img0001.jpg "Western Blot: Kir2.1 Antibody [NBP3-03675] - Analysis of extracts of various cell lines, using Kir2.1 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit")
![Western Blot: Kir2.1 Antibody [NBP2-87692] - Host: Rabbit. Target Name: KCNJ2. Sample Tissue: Human HepG2 Whole Cell lysates. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/Kir2.1-Antibody-Western-Blot-NBP2-87692-img0001.jpg "Western Blot: Kir2.1 Antibody [NBP2-87692] - Host: Rabbit. Target Name: KCNJ2. Sample Tissue: Human HepG2 Whole Cell lysates. Antibody Dilution: 1ug/ml")