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![Western Blot: Ras Antibody [NBP2-43784] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with RAS antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Ras-Antibody-Western-Blot-NBP2-43784-img0007.jpg "Western Blot: Ras Antibody [NBP2-43784] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with RAS antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Ras Antibody [NBP2-43784] - Rat urinary bladder. Ras antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Ras-Antibody-Immunohistochemistry-Paraffin-NBP2-43784-img0005.jpg)
![Western Blot: Ras Antibody [NBP2-43784] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RAS antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Ras-Antibody-Western-Blot-NBP2-43784-img0006.jpg)
![Immunohistochemistry-Paraffin: Ras Antibody [NBP2-43784] - Human colon carcinoma. Ras antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Ras-Antibody-Immunohistochemistry-Paraffin-NBP2-43784-img0004.jpg)
![Western Blot: Ras Antibody [NBP2-43784] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Ras antibody (NBP2-43784) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43784_rabbit-polyclonal-ras-antibody-91020242075910.jpg)
![Western Blot: Ras Antibody [NBP2-43784] - Ras antibody [N1N3] detects Ras protein by Western blot analysis. Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Ras antibody [N1N3] (NBP2-43784) diluted at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp2-43784_rabbit-polyclonal-ras-antibody-91020242075960.jpg)
![Western Blot: Ras Antibody [NBP2-43784] - Various tissue extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ras antibody (NBP2-43784) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. (GI: gastrointestinal)](http://images.novusbio.com/fullsize/nbp2-43784_rabbit-polyclonal-ras-antibody-91020242014644.jpg)
![Western Blot: Ras Antibody [NBP2-43784] - Ras antibody [N1N3] detects Ras protein by Western blot analysis. Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Ras antibody [N1N3] (NBP2-43784) diluted at a dilution of 1:500.](http://images.novusbio.com/fullsize/nbp2-43784_rabbit-polyclonal-ras-antibody-91020242041253.jpg)
![Western Blot: Ras Antibody (HL10) - G12D Mutant [NBP3-13647] - Non-transfected (-) and transfected (+) Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ras (G12D Mutant) antibody [HL10] (NBP3-13647) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Ras-Antibody-HL10-G12D-Mutant-Western-Blot-NBP3-13647-img0005.jpg "Western Blot: Ras Antibody (HL10) - G12D Mutant [NBP3-13647] - Non-transfected (-) and transfected (+) Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ras (G12D Mutant) antibody [HL10] (NBP3-13647) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Ras Antibody (HL10) - G12D Mutant [NBP3-13647] - Ras (G12D Mutant) antibody [HL10] detects Ras (G12D Mutant) protein at by immunohistochemical analysis. Sample: Paraffin-embedded KPC mouse pancreatic tumor cells implanted in the pancreas of B6 mice (left) and normal mouse pancreas (right). Ras (G12D Mutant) stained by Ras (G12D Mutant) antibody [HL10] (NBP3-13647) diluted at 1:75. Antigen Retrieval: Citrate buffer, pH 6.0, 40 min](http://images.novusbio.com/fullsize/Ras-Antibody-HL10-G12D-Mutant-Immunohistochemistry-Paraffin-NBP3-13647-img0003.jpg)
![Western Blot: Ras Antibody (HL10) - G12D Mutant [NBP3-13647] - Various whole cell extracts were separated by SDS-PAGE, and the membrane was blotted with Ras (G12D Mutant) antibody [HL10] (NBP3-13647) diluted at 1:1000.](http://images.novusbio.com/fullsize/Ras-Antibody-HL10-G12D-Mutant-Western-Blot-NBP3-13647-img0002.jpg)
![Western Blot: Ras Antibody (HL10) - G12D Mutant [NBP3-13647] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ras (G12D Mutant) antibody [HL10] (NBP3-13647) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.](http://images.novusbio.com/fullsize/Ras-Antibody-HL10-G12D-Mutant-Western-Blot-NBP3-13647-img0004.jpg)
![Immunohistochemistry-Paraffin: Ras Antibody (HL10) - G12D Mutant [NBP3-13647] - Ras (G12D Mutant) antibody [HL10] detects Ras (G12D Mutant) protein by immunohistochemical analysis. Sample: Paraffin-embedded human wild-type (left) and KRas G12D mutant (right) pancreatic tumor. Ras (G12D Mutant) stained by Ras (G12D Mutant) antibody [HL10] (NBP3-13647) diluted at 1:50.](http://images.novusbio.com/fullsize/Ras-Antibody-HL10-G12D-Mutant-Immunohistochemistry-Paraffin-NBP3-13647-img0001.jpg)
![ICC/IF: Ras Antibody (HL10) - G12D Mutant [NBP3-13647] - Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RAS (G12D Mutant) stained by RAS (G12D Mutant) antibody [HL10] diluted at 1:500.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/nbp3-13647_rabbit-ras-mab-hl10-g12d-mutant-immunocytochemistry-immunofluorescence-14720239940..jpg)
![Western Blot: Ras Antibody (HL169) - G12V Mutant [NBP3-13668] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RAS (G12V Mutant) antibody [HL169] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody](http://images.novusbio.com/fullsize/Ras-Antibody-HL169-G12V-Mutant-Western-Blot-NBP3-13668-img0007.jpg "Western Blot: Ras Antibody (HL169) - G12V Mutant [NBP3-13668] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RAS (G12V Mutant) antibody [HL169] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody")
![Immunohistochemistry-Paraffin: Ras Antibody (HL169) - G12V Mutant [NBP3-13668] - BxPC-3 xenograft (left) and CFPAC-1 xenograft (right). RAS (G12V Mutant) stained by RAS (G12V Mutant) antibody [HL169] diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Ras-Antibody-HL169-G12V-Mutant-Immunohistochemistry-Paraffin-NBP3-13668-img0004.jpg)
![Western Blot: Ras Antibody (HL169) - G12D Mutant [NBP3-13668] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ras (G12V Mutant) antibody [HL1] (NBP3-13668) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Ras-Antibody-HL169-G12D-Mutant-Western-Blot-NBP3-13668-img0002.jpg)
![Western Blot: Ras Antibody (HL169) - G12V Mutant [NBP3-13668] - Ras Antibody (HL169) - G12D Mutant [NBP3-13668] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ras (G12V Mutant) antibody [HL1] (NBP3-13668) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Ras-Antibody-HL169-G12D-Mutant-Western-Blot-NBP3-13668-img0003.jpg)
![Western Blot: Ras Antibody (3G8L8) [NBP3-15909] - Western blot analysis of extracts of various cell lines, using Ras Rabbit mAb (NBP3-15909) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Ras-Antibody-3G8L8-Western-Blot-NBP3-15909-img0003.jpg "Western Blot: Ras Antibody (3G8L8) [NBP3-15909] - Western blot analysis of extracts of various cell lines, using Ras Rabbit mAb (NBP3-15909) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunocytochemistry/Immunofluorescence: Ras Antibody (3G8L8) [NBP3-15909] - Immunofluorescence analysis of NIH-3T3 cells using Ras Rabbit mAb (NBP3-15909) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Ras-Antibody-3G8L8-Immunocytochemistry-Immunofluorescence-NBP3-15909-img0001.jpg)
![Western Blot: Ras Antibody (3G8L8) [NBP3-15909] - Western blot analysis of extracts of various cell lines, using Ras Rabbit mAb (NBP3-15909) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Ras-Antibody-3G8L8-Western-Blot-NBP3-15909-img0002.jpg)
![Western Blot: Ras Antibody (JE44-13) [NBP3-33138] - Western blot analysis of Ras on different lysates with Rabbit anti-Ras antibody (NBP3-33138) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (20 ug/Lane) Lane 2: Jurkat cell lysate (20 ug/Lane) Lane 3: SH-SY5Y cell lysate (20 ug/Lane) Lane 4: MCF7 cell lysate (20 ug/Lane) Lane 5: A431 cell lysate (20 ug/Lane) Lane 6: A375 cell lysate (20 ug/Lane) Lane 7: C6 cell lysate (20 ug/Lane) Lane 8: NIH/3T3 cell lysate (20 ug/Lane) Lane 9: Rat spleen tissue lysate (40 ug/Lane) Lane 10: Mouse spleen tissue lysate (40 ug/Lane) Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33138) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33138_rabbit-ras-mab-je44-13-28820241423314.jpg "Western Blot: Ras Antibody (JE44-13) [NBP3-33138] - Western blot analysis of Ras on different lysates with Rabbit anti-Ras antibody (NBP3-33138) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (20 ug/Lane) Lane 2: Jurkat cell lysate (20 ug/Lane) Lane 3: SH-SY5Y cell lysate (20 ug/Lane) Lane 4: MCF7 cell lysate (20 ug/Lane) Lane 5: A431 cell lysate (20 ug/Lane) Lane 6: A375 cell lysate (20 ug/Lane) Lane 7: C6 cell lysate (20 ug/Lane) Lane 8: NIH/3T3 cell lysate (20 ug/Lane) Lane 9: Rat spleen tissue lysate (40 ug/Lane) Lane 10: Mouse spleen tissue lysate (40 ug/Lane) Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33138) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: Ras Antibody (JE44-13) [NBP3-33138] - Immunocytochemistry analysis of MCF7 cells labeling Ras with Rabbit anti-Ras antibody (NBP3-33138) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ras antibody (NBP3-33138) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-33138_rabbit-ras-mab-je44-13-29202410334099.jpg)
![Flow Cytometry: Ras Antibody (JE44-13) [NBP3-33138] - Flow cytometric analysis of MCF7 cells labeling Ras. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33138, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33138_rabbit-ras-mab-je44-13-29202416372143.jpg)
![Western Blot: Ras Antibody - G12D Mutant [NBP3-13102] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ras (G12D Mutant Specific) antibody (NBP3-13102) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Ras-Antibody---G12D-Mutant-Western-Blot-NBP3-13102-img0002.jpg "Western Blot: Ras Antibody - G12D Mutant [NBP3-13102] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ras (G12D Mutant Specific) antibody (NBP3-13102) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Western Blot: Ras Antibody - G12D Mutant [NBP3-13102] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ras antibody (NBP3-13102) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13102_rabbit-ras-pab-g12d-mutant-91020242014625.jpg)
![Western Blot: Ras Antibody - G12V Mutant [NBP3-13132] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ras (G12V Mutant) antibody (NBP3-13132) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Ras-Antibody---G12V-Mutant-Western-Blot-NBP3-13132-img0002.jpg "Western Blot: Ras Antibody - G12V Mutant [NBP3-13132] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ras (G12V Mutant) antibody (NBP3-13132) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: Ras Antibody - G12V Mutant [NBP3-13132] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ras (G12V Mutant specific) antibody (NBP3-13132) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Ras-Antibody---G12V-Mutant-Western-Blot-NBP3-13132-img0001.jpg)
![Western Blot: Ras Antibody (HL1153) - G12A Mutant - Azide and BSA Free [NBP3-25622] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RAS (G12A mutant) antibody [HL1153] (NBP3-25622) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25622_rabbit-ras-mab-hl1153-g12a-mutant-azide-and-bsa-free-212024939198.jpg "Western Blot: Ras Antibody (HL1153) - G12A Mutant - Azide and BSA Free [NBP3-25622] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RAS (G12A mutant) antibody [HL1153] (NBP3-25622) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Ras Antibody (HL1153) - G12A Mutant - Azide and BSA Free [NBP3-25622] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RAS (G12A mutant) antibody [HL1153] (NBP3-25622) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25622_rabbit-ras-mab-hl1153-g12a-mutant-azide-and-bsa-free-212024939134.jpg)
![Western Blot: Ras Antibody - Q61R Mutant [NBP3-13101] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ras (Q61R Mutant Specific) antibody (NBP3-13101) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Ras-Antibody---Q61R-Mutant-Western-Blot-NBP3-13101-img0001.jpg "Western Blot: Ras Antibody - Q61R Mutant [NBP3-13101] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ras (Q61R Mutant Specific) antibody (NBP3-13101) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: Ras Antibody - A59T Mutant [NBP3-13100] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ras (A59T Mutant Specific) antibody (NBP3-13100) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Ras-Antibody---A59T-Mutant-Western-Blot-NBP3-13100-img0001.jpg "Western Blot: Ras Antibody - A59T Mutant [NBP3-13100] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ras (A59T Mutant Specific) antibody (NBP3-13100) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: Ras Antibody - G13D Mutant [NBP3-13103] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ras (G13D Mutant Specific) antibody (NBP3-13103) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Ras-Antibody---G13D-Mutant-Western-Blot-NBP3-13103-img0001.jpg "Western Blot: Ras Antibody - G13D Mutant [NBP3-13103] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ras (G13D Mutant Specific) antibody (NBP3-13103) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: Ras Antibody - K117N Mutant [NBP3-13104] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ras (K117N Mutant Specific) antibody (NBP3-13104) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Ras-Antibody---K117N-Mutant-Western-Blot-NBP3-13104-img0001.jpg "Western Blot: Ras Antibody - K117N Mutant [NBP3-13104] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ras (K117N Mutant Specific) antibody (NBP3-13104) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")