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![Western Blot: GRB2 Antibody (9K6H0) [NBP3-15638] - Analysis of extracts of various cell lines, using GRB2 antibody (NBP3-15638) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/GRB2-Antibody-9K6H0-Western-Blot-NBP3-15638-img0005.jpg "Western Blot: GRB2 Antibody (9K6H0) [NBP3-15638] - Analysis of extracts of various cell lines, using GRB2 antibody (NBP3-15638) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Immunocytochemistry/Immunofluorescence: GRB2 Antibody (9K6H0) [NBP3-15638] - Immunofluorescence analysis of HeLa cells using GRB2 Rabbit mAb (NBP3-15638) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/GRB2-Antibody-9K6H0-Immunocytochemistry-Immunofluorescence-NBP3-15638-img0004.jpg)
![Immunohistochemistry-Paraffin: GRB2 Antibody (9K6H0) [NBP3-15638] - Mouse spleen using GRB2 Rabbit mAb (NBP3-15638) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/GRB2-Antibody-9K6H0-Immunohistochemistry-Paraffin-NBP3-15638-img0003.jpg)
![Immunohistochemistry-Paraffin: GRB2 Antibody (9K6H0) [NBP3-15638] - Rat spleen using GRB2 Rabbit mAb (NBP3-15638) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/GRB2-Antibody-9K6H0-Immunohistochemistry-Paraffin-NBP3-15638-img0001.jpg)
![Immunohistochemistry-Paraffin: GRB2 Antibody (9K6H0) [NBP3-15638] - Human lung cancer using GRB2 Rabbit mAb (NBP3-15638) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/GRB2-Antibody-9K6H0-Immunohistochemistry-Paraffin-NBP3-15638-img0002.jpg)
![Immunoprecipitation: GRB2 Antibody (9K6H0) [NBP3-15638] - Analysis of 300ug extracts of 293T cells using 3ug GRB2 antibody (NBP3-15638). Western blot was performed from the immunoprecipitate using GRB2 (NBP3-15638) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/GRB2-Antibody-9K6H0-Immunoprecipitation-NBP3-15638-img0006.jpg)
![Western Blot: GRB2 Antibody [NB100-866] - (0.05ug/ml) staining of Mouse (A+C) and Rat (B+D) Brain (A+B) and Spleen (C+D) lysates (35ug total protein in RIPA buffer). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.](http://images.novusbio.com/fullsize/GRB2-Antibody-Western-Blot-NB100-866-img0012.jpg "Western Blot: GRB2 Antibody [NB100-866] - (0.05ug/ml) staining of Mouse (A+C) and Rat (B+D) Brain (A+B) and Spleen (C+D) lysates (35ug total protein in RIPA buffer). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.")
![Immunohistochemistry-Paraffin: GRB2 Antibody [NB100-866] - Negative Control showing staining of paraffin embedded Human Lymph Node, with no primary antibody.](http://images.novusbio.com/fullsize/GRB2-Antibody-Immunohistochemistry-Paraffin-NB100-866-img0014.jpg)
![Western Blot: GRB2 Antibody [NB100-866] - (0.03ug/ml) staining of Human (A) and Pig (B) Spleen lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/GRB2-Antibody-Western-Blot-NB100-866-img0010.jpg)
![Western Blot: GRB2 Antibody [NB100-866] - (0.05ug/ml) staining of Human Thymus (A) and Molt4 (B) lysates (35ug total protein in RIPA buffer). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.](http://images.novusbio.com/fullsize/GRB2-Antibody-Western-Blot-NB100-866-img0011.jpg)
![Immunohistochemistry-Paraffin: GRB2 Antibody [NB100-866] - (1ug/ml) staining of paraffin embedded Human Lymph Node. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/GRB2-Antibody-Immunohistochemistry-Paraffin-NB100-866-img0013.jpg)
![Western Blot: Recombinant Human GRB2 Protein [H00002885-P01] - Analysis of GST-GRB2 in GRB2 recombinant protein. Image from verified customer review.](http://images.novusbio.com/fullsize/Recombinant-Human-GRB2-Protein-Western-Blot-H00002885-P01-img0003.jpg "Western Blot: Recombinant Human GRB2 Protein [H00002885-P01] - Analysis of GST-GRB2 in GRB2 recombinant protein. Image from verified customer review.")
![SDS-Page: Recombinant Human GRB2 Protein [H00002885-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-GRB2-Protein-SDS-Page-H00002885-P01-img0004.jpg)
![Western Blot: GRB2 Antibody (JE49-73) [NBP3-32403] - Western blot analysis of GRB2 on different lysates with Rabbit anti-GRB2 antibody (NBP3-32403) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: A431 cell lysate Lane 4: MCF7 cell lysate Lane 5: HCT 116 cell lysate Lane 6: COS-1 cell lysate Lane 7: RAW264.7 cell lysate Lane 8: NIH/3T3 cell lysate Lane 9: C6 cell lysate Lane 10: PC-12 cell lysate Lane 11: Human lung tissue lysate Lane 12: Mouse testis tissue lysate Lane 13: Rat testis tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 25 kDa Observed band size: 25 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32403) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32403_rabbit-grb2-mab-je49-73-278202415295585.jpg "Western Blot: GRB2 Antibody (JE49-73) [NBP3-32403] - Western blot analysis of GRB2 on different lysates with Rabbit anti-GRB2 antibody (NBP3-32403) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: A431 cell lysate Lane 4: MCF7 cell lysate Lane 5: HCT 116 cell lysate Lane 6: COS-1 cell lysate Lane 7: RAW264.7 cell lysate Lane 8: NIH/3T3 cell lysate Lane 9: C6 cell lysate Lane 10: PC-12 cell lysate Lane 11: Human lung tissue lysate Lane 12: Mouse testis tissue lysate Lane 13: Rat testis tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 25 kDa Observed band size: 25 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32403) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: GRB2 Antibody (JE49-73) [NBP3-32403] - Immunocytochemistry analysis of PC-12 cells labeling GRB2 with Rabbit anti-GRB2 antibody (NBP3-32403) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GRB2 antibody (NBP3-32403) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32403_rabbit-grb2-mab-je49-73-29202410334095.jpg)
![Flow Cytometry: GRB2 Antibody (JE49-73) [NBP3-32403] - Flow cytometric analysis of MCF7 cells labeling GRB2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32403, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32403_rabbit-grb2-mab-je49-73-29202416372159.jpg)
![Western Blot: GRB2 Antibody (6E1) [NBP3-15167] - Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Mouse Brain whole cell lysate, Rat Brain whole cell lysate. All lanes: GRB2 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 26, 21 KDa. Observed band size: 28 KDa.](http://images.novusbio.com/fullsize/GRB2-Antibody-6E1-Western-Blot-NBP3-15167-img0002.jpg "Western Blot: GRB2 Antibody (6E1) [NBP3-15167] - Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Mouse Brain whole cell lysate, Rat Brain whole cell lysate. All lanes: GRB2 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 26, 21 KDa. Observed band size: 28 KDa.")
![Immunohistochemistry: GRB2 Antibody (6E1) [NBP3-15167] - IHC image of GRB2 antibody diluted at 1:100 and staining in paraffin-embedded human ovarian cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/GRB2-Antibody-6E1-Immunohistochemistry-NBP3-15167-img0001.jpg)
![Western Blot: GRB2 Antibody (SR1396) [NBP3-21851] - Western blot analysis using NBP3-21851 in HEK293 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21851_rabbit-grb2-mab-sr1396-57202315545124.jpg "Western Blot: GRB2 Antibody (SR1396) [NBP3-21851] - Western blot analysis using NBP3-21851 in HEK293 cell lysate.")
![Western Blot: GRB2 Antibody [NBP2-55208] - Analysis using Anti-GRB2 antibody NBP2-55208 (A) shows similar pattern to independent antibody NBP2-55209 (B).](http://images.novusbio.com/fullsize/GRB2-Antibody-Western-Blot-NBP2-55208-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: GRB2 Antibody [NBP2-55208] - Analysis using Anti-GRB2 antibody NBP2-55208 (A) shows similar pattern to independent antibody NBP2-55209 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: GRB2 Antibody [NBP2-55208] - Staining of human cell line PC-3 shows localization to nucleus & nucleoli.](http://images.novusbio.com/fullsize/GRB2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55208-img0001.jpg)
![Western Blot: GRB2 Antibody [NBP2-55209] - Analysis using Anti-GRB2 antibody NBP2-55209 (A) shows similar pattern to independent antibody NBP2-55208 (B).](http://images.novusbio.com/fullsize/GRB2-Antibody-Western-Blot-NBP2-55209-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: GRB2 Antibody [NBP2-55209] - Analysis using Anti-GRB2 antibody NBP2-55209 (A) shows similar pattern to independent antibody NBP2-55208 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: GRB2 Antibody [NBP2-55209] - Staining of human cell line PC-3 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/GRB2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55209-img0001.jpg)