| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44210 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44209 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-04094-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76544 | |||
![Western Blot: GLS2 Antibody [NBP1-76544] - Glutaminase protein expression in PC3 and PC3M cell lines before/after CB-839 treatment. PC3 & PC3M cells were treated (+) or not treated (-) with GLS1 inhibitor CB-839 (1 uM) and their expression of GLS1 & GLS2 proteins determined by Western blot. Antibodies specific for GAC & KCA were utilized on two replicate samples, while GLS2 expression was determined on three. PC3 cell lysates expressed GLS2 more than PC3M cell lysates regardless of CB-839 treatment. The difference in GLS2 expression was significant in the untreated cells (P?<?0.03) but because of high variability the difference was not significant in the drug-treated cells. Levels of GLS1 (GAC and KGA) were similar in all cell lysates. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-16327-z), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GLS2-Antibody-Western-Blot-NBP1-76544-img0009.jpg "Western Blot: GLS2 Antibody [NBP1-76544] - Glutaminase protein expression in PC3 and PC3M cell lines before/after CB-839 treatment. PC3 & PC3M cells were treated (+) or not treated (-) with GLS1 inhibitor CB-839 (1 uM) and their expression of GLS1 & GLS2 proteins determined by Western blot. Antibodies specific for GAC & KCA were utilized on two replicate samples, while GLS2 expression was determined on three. PC3 cell lysates expressed GLS2 more than PC3M cell lysates regardless of CB-839 treatment. The difference in GLS2 expression was significant in the untreated cells (P?<?0.03) but because of high variability the difference was not significant in the drug-treated cells. Levels of GLS1 (GAC and KGA) were similar in all cell lysates. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-16327-z), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: GLS2 Antibody [NBP1-76544] - of GLS2 in Human Kidney cells. Dilution 20 ug/mL.](http://images.novusbio.com/fullsize/GLS2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76544-img0007.jpg)
![Immunohistochemistry: GLS2 Antibody [NBP1-76544] - Immunohistochemistry of GLS2 in human kidney tissue with GLS2 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/GLS2-Antibody-Immunohistochemistry-NBP1-76544-img0003.jpg)
![Simple Western: GLS2 Antibody [NBP1-76544] - Simple Western lane view shows a specific band for GLS2 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/GLS2-Antibody-Simple-Western-NBP1-76544-img0008.jpg)
![Immunocytochemistry/ Immunofluorescence: GLS2 Antibody - BSA Free [NBP1-76544] - Histological analyses for assessing response to bevacizumab treatment. A Immunofluorescence staining of tumor vasculature & integrin beta 3 with antibodies against CD31 (green) & CD61 (integrin beta 3, red) in the treated & control groups at the end of the study. B Quantitative analysis of integrin beta 3 & CD31-positive area fraction of tumors between the control & treatment groups. **P < 0.05. Magnification: ×200. Microvascular density (CD31) from the therapy group (C) & control group (D). Tumor cell proliferation (Ki-67) from the therapy group (F) & control group (G). Quantitative MVD (E) & Ki67 (H) assessment of therapy & control group. Error bars denote standard errors, Mann–Whitney U-test were applied, **P < 0.05. Magnification: ×200 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31604441), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-76544_rabbit-polyclonal-gls2-antibody-310202416163769.jpg)
![Western Blot: GLS2 Antibody [NBP2-82071] - Western blot analysis of GLS2 in rat liver tissue lysate with GLS2 antibody at (A) 1 and (B) 2 ug/mL](http://images.novusbio.com/fullsize/GLS2-Antibody-Western-Blot-NBP2-82071-img0001.jpg "Western Blot: GLS2 Antibody [NBP2-82071] - Western blot analysis of GLS2 in rat liver tissue lysate with GLS2 antibody at (A) 1 and (B) 2 ug/mL")
![Immunocytochemistry/ Immunofluorescence: GLS2 Antibody - BSA Free [NBP2-82071] - Immunofluorescence of GLS2 in mouse brain tissue with GLS2 Antibody at 20 ug/mL.Green: GLS2 antibody Red: Phylloidin staining Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp2-82071_rabbit-polyclonal-gls2-antibody-2711202419553076.gif)
![Immunohistochemistry: GLS2 Antibody - BSA Free [NBP2-82071] - Immunohistochemistry of GLS2 in rat liver tissue with GLS2 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-82071_rabbit-polyclonal-gls2-antibody-2711202420141987.jpg)
![Immunohistochemistry: GLS2 Antibody - BSA Free [NBP2-82071] - Immunohistochemistry of GLS2 in mouse brain tissue with GLS2 Antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-82071_rabbit-polyclonal-gls2-antibody-2711202419553041.gif)
![Immunocytochemistry/ Immunofluorescence: GLS2 Antibody - BSA Free [NBP2-82071] - Immunofluorescence of GLS2 in rat liver cells with GLS2 antibody at 20 5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-82071_rabbit-polyclonal-gls2-antibody-2711202420141910.jpg)
![Western Blot: GLS2 Antibody (CL7721) [NBP2-88908] - Analysis in human cell line Daudi.](http://images.novusbio.com/fullsize/GLS2-Antibody-CL7721-Western-Blot-NBP2-88908-img0006.jpg "Western Blot: GLS2 Antibody (CL7721) [NBP2-88908] - Analysis in human cell line Daudi.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GLS2 Antibody (CL7721) [NBP2-88908] - Analysis in human liver and skeletal muscle tissues using NBP2-88908 antibody. Corresponding GLS2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/GLS2-Antibody-CL7721-Immunohistochemistry-Paraffin-NBP2-88908-img0005.jpg)
![Immunohistochemistry-Paraffin: GLS2 Antibody (CL7721) [NBP2-88908] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/GLS2-Antibody-CL7721-Immunohistochemistry-Paraffin-NBP2-88908-img0001.jpg)
![Immunohistochemistry-Paraffin: GLS2 Antibody (CL7721) [NBP2-88908] - Staining of human pancreas shows moderate granular cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/GLS2-Antibody-CL7721-Immunohistochemistry-Paraffin-NBP2-88908-img0002.jpg)
![Immunohistochemistry-Paraffin: GLS2 Antibody (CL7721) [NBP2-88908] - Staining of human prostate shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/GLS2-Antibody-CL7721-Immunohistochemistry-Paraffin-NBP2-88908-img0003.jpg)
![Immunohistochemistry-Paraffin: GLS2 Antibody (CL7721) [NBP2-88908] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/GLS2-Antibody-CL7721-Immunohistochemistry-Paraffin-NBP2-88908-img0004.jpg)
![Immunohistochemistry-Paraffin: GLS2 Antibody (CL7716) [NBP2-88907] - Analysis in human liver and skeletal muscle tissues using NBP2-88907 antibody. Corresponding GLS2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/GLS2-Antibody-CL7716-Immunohistochemistry-Paraffin-NBP2-88907-img0005.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GLS2 Antibody (CL7716) [NBP2-88907] - Analysis in human liver and skeletal muscle tissues using NBP2-88907 antibody. Corresponding GLS2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GLS2 Antibody (CL7716) [NBP2-88907] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/GLS2-Antibody-CL7716-Immunohistochemistry-Paraffin-NBP2-88907-img0004.jpg)
![Immunohistochemistry-Paraffin: GLS2 Antibody (CL7716) [NBP2-88907] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/GLS2-Antibody-CL7716-Immunohistochemistry-Paraffin-NBP2-88907-img0001.jpg)
![Immunohistochemistry-Paraffin: GLS2 Antibody (CL7716) [NBP2-88907] - Staining of human pancreas shows moderate granular cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/GLS2-Antibody-CL7716-Immunohistochemistry-Paraffin-NBP2-88907-img0002.jpg)
![Immunohistochemistry-Paraffin: GLS2 Antibody (CL7716) [NBP2-88907] - Staining of human prostate shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/GLS2-Antibody-CL7716-Immunohistochemistry-Paraffin-NBP2-88907-img0003.jpg)
![Western Blot: GLS2 Antibody [NBP1-54773] - Sample Tissue: Human NCI-H226 Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/GLS2-Antibody-Western-Blot-NBP1-54773-img0006.jpg "Western Blot: GLS2 Antibody [NBP1-54773] - Sample Tissue: Human NCI-H226 Antibody Dilution: 1.0 ug/ml")
![Immunohistochemistry: GLS2 Antibody [NBP1-54773] - Human kidney](http://images.novusbio.com/fullsize/GLS2-Antibody-Immunohistochemistry-NBP1-54773-img0005.jpg)
![Western Blot: GLS2 Antibody [NBP1-54773] - Titration: 2.5 ug/ml Positive Control: HepG2 cell lysate.](http://images.novusbio.com/fullsize/GLS2-Antibody-Western-Blot-NBP1-54773-img0004.jpg)
![Western Blot: GLS2 Antibody [NBP3-04094] - Analysis of extracts of various cell lines, using GLS2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/GLS2-Antibody-Western-Blot-NBP3-04094-img0001.jpg "Western Blot: GLS2 Antibody [NBP3-04094] - Analysis of extracts of various cell lines, using GLS2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/ Immunofluorescence: GLS2 Antibody - Azide and BSA Free [NBP3-04094] - Immunofluorescence analysis of U2OS cells using GLS2 Rabbit pAb (A16029) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-04094_rabbit-polyclonal-gls2-antibody-52202519302991.jpg)
![Western Blot: GLS2 Overexpression Lysate (Adult Normal) [NBP2-07429] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GLS2.](http://images.novusbio.com/fullsize/GLS2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07429-img0001.jpg "Western Blot: GLS2 Overexpression Lysate (Adult Normal) [NBP2-07429] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GLS2.")