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![Western Blot: GLI-3 Antibody [NBP2-29627] - Adult mouse brain tissue lysates. Image from verified customer review.](http://images.novusbio.com/fullsize/GLI-3-Antibody-Western-Blot-NBP2-29627-img0017.jpg "Western Blot: GLI-3 Antibody [NBP2-29627] - Adult mouse brain tissue lysates. Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: GLI-3 Antibody [NBP2-29627] - HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/GLI-3-Antibody-Immunofluorescence-NBP2-29627-img0012.jpg)
![Immunohistochemistry-Paraffin: GLI-3 Antibody [NBP2-29627] - Analysis of in mouse liver.](http://images.novusbio.com/fullsize/GLI-3-Antibody-Immunohistochemistry-Paraffin-NBP2-29627-img0015.jpg)
![Flow (Intracellular): GLI-3 Antibody [NBP2-29627] - Intracellular staining for GLI-3 in untreated human B cell lines (BCWM.1, MWCL-1 and RPCI-WM1) using anti-GLI-3 antibody. Rabbit IgG Isotype Control (Cat# NBP2-36463) was used as a negative control. Image courtesy of Sherine Elsawa, Northern Illinois University.](http://images.novusbio.com/fullsize/GLI-3-Antibody-Flow-Intracellular-NBP2-29627-img0010.jpg)
![Western Blot: GLI-3 Antibody [NBP2-29627] - Analysis of GLI-3 in Hela cell lysate and MEF cell lysate.](http://images.novusbio.com/fullsize/GLI-3-Antibody-Western-Blot-NBP2-29627-img0016.jpg)
![Immunohistochemistry-Paraffin: GLI-3 Antibody [NBP2-29627] - Analysis of in mouse testis.](http://images.novusbio.com/fullsize/GLI-3-Antibody-Immunohistochemistry-Paraffin-NBP2-29627-img0014.jpg)
![Western Blot: GLI-3 Antibody [NBP2-16665] - A: Jurkat.](http://images.novusbio.com/fullsize/GLI-3-Antibody-Western-Blot-NBP2-16665-img0008.jpg "Western Blot: GLI-3 Antibody [NBP2-16665] - A: Jurkat.")
![Immunocytochemistry/Immunofluorescence: GLI-3 Antibody [NBP2-16665] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/GLI-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16665-img0005.jpg)
![Immunohistochemistry-Paraffin: GLI-3 Antibody [NBP2-16665] - Human lung adenocarcinoma. GLI3 antibody diluted at 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/GLI-3-Antibody-Immunohistochemistry-Paraffin-NBP2-16665-img0009.jpg)
![Western Blot: GLI-3 Antibody [NBP2-16665] - A. PC-12 whole cell lysate/extract B. Rat whole cell lysate/extract.](http://images.novusbio.com/fullsize/GLI-3-Antibody-Western-Blot-NBP2-16665-img0006.jpg)
![Western Blot: GLI-3 Antibody [NBP2-16665] - A: mouse ESC.](http://images.novusbio.com/fullsize/GLI-3-Antibody-Western-Blot-NBP2-16665-img0007.jpg)
![Western Blot: GLI-3 Antibody (8B1E2) [NBP3-16166] - Western blot analysis of extracts of HeLa cells, using GLI-3 antibody (NBP3-16166) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/GLI-3-Antibody-8B1E2-Western-Blot-NBP3-16166-img0003.jpg "Western Blot: GLI-3 Antibody (8B1E2) [NBP3-16166] - Western blot analysis of extracts of HeLa cells, using GLI-3 antibody (NBP3-16166) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunocytochemistry/Immunofluorescence: GLI-3 Antibody (8B1E2) [NBP3-16166] - Immunofluorescence analysis of U-2 OS cells using GLI-3 Rabbit mAb (NBP3-16166) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/GLI-3-Antibody-8B1E2-Immunocytochemistry-Immunofluorescence-NBP3-16166-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: GLI-3 Antibody (8B1E2) [NBP3-16166] - Immunofluorescence analysis of C6 cells using GLI-3 Rabbit mAb (NBP3-16166) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/GLI-3-Antibody-8B1E2-Immunocytochemistry-Immunofluorescence-NBP3-16166-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: GLI-3 Antibody (2C9) [H00002737-M01] - Analysis of monoclonal antibody to GLI3 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/GLI-3-Antibody-2C9-Immunocytochemistry-Immunofluorescence-H00002737-M01-img0004.jpg "Immunocytochemistry/Immunofluorescence: GLI-3 Antibody (2C9) [H00002737-M01] - Analysis of monoclonal antibody to GLI3 on HeLa cell . Antibody concentration 10 ug/ml.")
![Proximity Ligation Assay: GLI-3 Antibody (2C9) [H00002737-M01] - Analysis of protein-protein interactions between SMAD1 and GLI3. HeLa cells were stained with anti-SMAD1 rabbit purified polyclonal 1:1200 and anti-GLI3 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/GLI-3-Antibody-2C9-Proximity-Ligation-Assay-H00002737-M01-img0002.jpg)
![ELISA: GLI-3 Antibody (2C9) [H00002737-M01] - Detection limit for recombinant GST tagged GLI3 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/GLI-3-Antibody-2C9-ELISA-H00002737-M01-img0006.jpg)
![Immunohistochemistry-Paraffin: GLI-3 Antibody [NBP1-89275] - Staining of human pancreas shows no nuclear positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/GLI-3-Antibody-Immunohistochemistry-Paraffin-NBP1-89275-img0007.jpg "Immunohistochemistry-Paraffin: GLI-3 Antibody [NBP1-89275] - Staining of human pancreas shows no nuclear positivity in exocrine glandular cells as expected.")
![Immunohistochemistry-Paraffin: GLI-3 Antibody [NBP1-89275] - Staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.](http://images.novusbio.com/fullsize/GLI-3-Antibody-Immunohistochemistry-Paraffin-NBP1-89275-img0004.jpg)
![Immunohistochemistry-Paraffin: GLI-3 Antibody [NBP1-89275] - Staining of human spleen shows moderate to strong nuclear positivity in cells in red pulp.](http://images.novusbio.com/fullsize/GLI-3-Antibody-Immunohistochemistry-Paraffin-NBP1-89275-img0006.jpg)
![SDS-Page: Recombinant Human GLI-3 Protein [H00002737-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00002737-Q01-1.jpg "SDS-Page: Recombinant Human GLI-3 Protein [H00002737-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: GLI-3 Antibody (S08-2B9) [NBP3-20039] - Western blot detection of GLI-3 in 293 cell lysates using NBP3-20039 (1:1000 diluted). Predicted band size: 170kDa. Observed band size: 200kDa.](http://images.novusbio.com/fullsize/GLI-3-Antibody-S08-2B9-Western-Blot-NBP3-20039-img0001.jpg "Western Blot: GLI-3 Antibody (S08-2B9) [NBP3-20039] - Western blot detection of GLI-3 in 293 cell lysates using NBP3-20039 (1:1000 diluted). Predicted band size: 170kDa. Observed band size: 200kDa.")