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![Immunohistochemistry-Paraffin: alpha-Galactosidase A/GLA Antibody [NBP1-89796] - Analysis in human seminal vesicle and skeletal muscle tissues. Corresponding GLA RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/alpha-Galactosidase-A-GLA-Antibody-Immunohistochemistry-Paraffin-NBP1-89796-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: alpha-Galactosidase A/GLA Antibody [NBP1-89796] - Analysis in human seminal vesicle and skeletal muscle tissues. Corresponding GLA RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: alpha-Galactosidase A/GLA Antibody [NBP1-89796] - Staining of human adrenal gland, colon, liver and testis using Anti-GLA antibody NBP1-89796 (A) shows similar protein distribution across tissues to independent antibody NBP1-89797 (B).](http://images.novusbio.com/fullsize/alpha-Galactosidase-A-GLA-Antibody-Immunohistochemistry-Paraffin-NBP1-89796-img0005.jpg)
![Immunohistochemistry-Paraffin: alpha-Galactosidase A/GLA Antibody [NBP1-89796] - Staining of human duodenum shows moderate positivity in lysosomes in glandular cells.](http://images.novusbio.com/fullsize/alpha-Galactosidase-A-GLA-Antibody-Immunohistochemistry-Paraffin-NBP1-89796-img0008.jpg)
![Immunohistochemistry-Paraffin: alpha-Galactosidase A/GLA Antibody [NBP1-89796] - Staining of human fallopian tube shows moderate positivity in lysosomes in glandular cells.](http://images.novusbio.com/fullsize/alpha-Galactosidase-A-GLA-Antibody-Immunohistochemistry-Paraffin-NBP1-89796-img0009.jpg)
![Immunohistochemistry-Paraffin: alpha-Galactosidase A/GLA Antibody [NBP1-89796] - Staining of human testis shows moderate positivity in lysosomes in Leydig cells.](http://images.novusbio.com/fullsize/alpha-Galactosidase-A-GLA-Antibody-Immunohistochemistry-Paraffin-NBP1-89796-img0013.jpg)
![Immunohistochemistry-Paraffin: alpha-Galactosidase A/GLA Antibody [NBP1-89796] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/alpha-Galactosidase-A-GLA-Antibody-Immunohistochemistry-Paraffin-NBP1-89796-img0011.jpg)
![Immunohistochemistry-Paraffin: alpha-Galactosidase A/GLA Antibody [NBP1-89796] - Staining of human seminal vesicle shows moderate to strong positivity in lysosomes in glandular cells.](http://images.novusbio.com/fullsize/alpha-Galactosidase-A-GLA-Antibody-Immunohistochemistry-Paraffin-NBP1-89796-img0012.jpg)
![Immunohistochemistry-Paraffin: alpha-Galactosidase A/GLA Antibody [NBP1-89796] - Staining of human adrenal gland using Anti-GLA antibody.](http://images.novusbio.com/fullsize/alpha-Galactosidase-A-GLA-Antibody-Immunohistochemistry-Paraffin-NBP1-89796-img0014.jpg)
![Immunohistochemistry-Paraffin: alpha-Galactosidase A/GLA Antibody [NBP1-89796] - Staining of human colon using Anti-GLA antibody.](http://images.novusbio.com/fullsize/alpha-Galactosidase-A-GLA-Antibody-Immunohistochemistry-Paraffin-NBP1-89796-img0015.jpg)
![Immunohistochemistry-Paraffin: alpha-Galactosidase A/GLA Antibody [NBP1-89796] - Staining of human liver using Anti-GLA antibody.](http://images.novusbio.com/fullsize/alpha-Galactosidase-A-GLA-Antibody-Immunohistochemistry-Paraffin-NBP1-89796-img0016.jpg)
![Immunohistochemistry-Paraffin: alpha-Galactosidase A/GLA Antibody [NBP1-89797] - Staining of human colon.](http://images.novusbio.com/fullsize/alpha-Galactosidase-A-GLA-Antibody-Immunohistochemistry-Paraffin-NBP1-89797-img0011.jpg "Immunohistochemistry-Paraffin: alpha-Galactosidase A/GLA Antibody [NBP1-89797] - Staining of human colon.")
![Immunohistochemistry: alpha-Galactosidase A/GLA Antibody [NBP1-89797] - Staining of human adrenal gland shows strong granular positivity in cytoplasm of cortical cells.](http://images.novusbio.com/fullsize/alpha-Galactosidase-A-GLA-Antibody-Immunohistochemistry-NBP1-89797-img0003.jpg)
![Immunohistochemistry-Paraffin: alpha-Galactosidase A/GLA Antibody [NBP1-89797] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/alpha-Galactosidase-A-GLA-Antibody-Immunohistochemistry-Paraffin-NBP1-89797-img0004.jpg)
![Immunohistochemistry-Paraffin: alpha-Galactosidase A/GLA Antibody [NBP1-89797] - Staining of human seminal vesicle shows high expression.](http://images.novusbio.com/fullsize/alpha-Galactosidase-A-GLA-Antibody-Immunohistochemistry-Paraffin-NBP1-89797-img0005.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: alpha-Galactosidase A/GLA Antibody [NBP1-89797] - Staining in human seminal vesicle and pancreas tissues using anti-GLA antibody. Corresponding GLA RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/alpha-Galactosidase-A-GLA-Antibody-Immunohistochemistry-Paraffin-NBP1-89797-img0006.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: alpha-Galactosidase A/GLA Antibody [NBP1-89797] - Staining of human adrenal gland, colon, liver and testis using Anti-GLA antibody NBP1-89797 (A) shows similar protein distribution across tissues to independent antibody NBP1-89796 (B).](http://images.novusbio.com/fullsize/alpha-Galactosidase-A-GLA-Antibody-Immunohistochemistry-Paraffin-NBP1-89797-img0007.jpg)
![Immunohistochemistry-Paraffin: alpha-Galactosidase A/GLA Antibody [NBP1-89797] - Staining of human liver.](http://images.novusbio.com/fullsize/alpha-Galactosidase-A-GLA-Antibody-Immunohistochemistry-Paraffin-NBP1-89797-img0008.jpg)
![Immunohistochemistry-Paraffin: alpha-Galactosidase A/GLA Antibody [NBP1-89797] - Staining of human testis.](http://images.novusbio.com/fullsize/alpha-Galactosidase-A-GLA-Antibody-Immunohistochemistry-Paraffin-NBP1-89797-img0009.jpg)
![Immunohistochemistry-Paraffin: alpha-Galactosidase A/GLA Antibody [NBP1-89797] - Staining of human adrenal gland.](http://images.novusbio.com/fullsize/alpha-Galactosidase-A-GLA-Antibody-Immunohistochemistry-Paraffin-NBP1-89797-img0010.jpg)
![Western Blot: alpha-Galactosidase A/GLA Antibody [NBP2-16576] - Whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Galactosidase alpha antibody at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/alpha-Galactosidase-A-GLA-Antibody-Western-Blot-NBP2-16576-img0008.jpg "Western Blot: alpha-Galactosidase A/GLA Antibody [NBP2-16576] - Whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Galactosidase alpha antibody at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: alpha-Galactosidase A/GLA Antibody [NBP2-16576] - Analysis of paraffin-embedded Cal27 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/alpha-Galactosidase-A-GLA-Antibody-Immunohistochemistry-Paraffin-NBP2-16576-img0009.jpg)
![Western Blot: alpha-Galactosidase A/GLA Antibody [NBP2-16576] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/alpha-Galactosidase-A-GLA-Antibody-Western-Blot-NBP2-16576-img0010.jpg)
![Western Blot: alpha-Galactosidase A/GLA Antibody [NBP2-16576] - Rat tissue extracts (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Galactosidase alpha antibody [N1C2] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/alpha-Galactosidase-A-GLA-Antibody-Western-Blot-NBP2-16576-img0006.jpg)
![Immunoprecipitation: alpha-Galactosidase A/GLA Antibody [NBP2-16576] - Immunoprecipitation of GLA protein from 293T whole cell extracts using 5 ug of Galactosidase alpha antibody [N1C2]. Western blot analysis was performed using Galactosidase alpha antibody [N1C2]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/alpha-Galactosidase-A-GLA-Antibody-Immunoprecipitation-NBP2-16576-img0004.jpg)
![Western Blot: alpha-Galactosidase A/GLA Antibody [H00002717-D01] - Expression in transfected 293T cell line. Lane 1: GLA transfected lysate(48.8 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/alpha-Galactosidase-A-GLA-Antibody-Western-Blot-H00002717-D01-img0004.jpg "Western Blot: alpha-Galactosidase A/GLA Antibody [H00002717-D01] - Expression in transfected 293T cell line. Lane 1: GLA transfected lysate(48.8 KDa). Lane 2: Non-transfected lysate.")
![Western Blot: alpha-Galactosidase A/GLA Antibody [H00002717-D01] - Expression in HeLa.](http://images.novusbio.com/fullsize/alpha-Galactosidase-A-GLA-Antibody-Western-Blot-H00002717-D01-img0003.jpg)
![Immunoprecipitation: alpha-Galactosidase A/GLA Antibody [H00002717-D01] - GLA transfected lysate using anti-GLA MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with GLA purified MaxPab mouse polyclonal antibody.](http://images.novusbio.com/fullsize/alpha-Galactosidase-A-GLA-Antibody-Immunoprecipitation-H00002717-D01-img0005.jpg)
![Western Blot: alpha-Galactosidase A/GLA Antibody (7G8T9) [NBP3-16563] - Analysis of extracts of various cell lines, using alpha-Galactosidase A/GLA (GLA) Rabbit mAb (NBP3-16563) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/alpha-Galactosidase-A-GLA-Antibody-7G8T9-Western-Blot-NBP3-16563-img0001.jpg "Western Blot: alpha-Galactosidase A/GLA Antibody (7G8T9) [NBP3-16563] - Analysis of extracts of various cell lines, using alpha-Galactosidase A/GLA (GLA) Rabbit mAb (NBP3-16563) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry: alpha-Galactosidase A/GLA Antibody (30H11) [NBP3-26552] - Image of alpha-Galactosidase A/GLA Antibody (30H11) diluted at 1:100 and staining in paraffin-embedded human rectal cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.14% DAB.](http://images.novusbio.com/fullsize/nbp3-26552_rabbit-alpha-galactosidase-a-gla-mab-30h11-26220241510617.jpg "Immunohistochemistry: alpha-Galactosidase A/GLA Antibody (30H11) [NBP3-26552] - Image of alpha-Galactosidase A/GLA Antibody (30H11) diluted at 1:100 and staining in paraffin-embedded human rectal cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.14% DAB.")
![Flow Cytometry: alpha-Galactosidase A/GLA Antibody (30H11) [NBP3-26552] - Overlay Peak curve showing Hela cells stained with alpha-Galactosidase A/GLA Antibody (30H11) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26552_rabbit-alpha-galactosidase-a-gla-mab-30h11-282202414312742.jpg)
![Western Blot: alpha-Galactosidase A/GLA Antibody [NBP1-58018] - Human Brain lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/alpha-Galactosidase-A-GLA-Antibody-Western-Blot-NBP1-58018-img0003.jpg "Western Blot: alpha-Galactosidase A/GLA Antibody [NBP1-58018] - Human Brain lysate, concentration 0.2-1 ug/ml.")
![ELISA: Human alpha-Galactosidase A/GLA ELISA Kit (Colorimetric) [NBP3-23444] - Samples were spiked with high concentrations of alpha-Galactosidase A/GLA and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-23444_human-alpha-galactosidase-a-gla-elisa-kit-colorimetric-132202416201940.jpg "ELISA: Human alpha-Galactosidase A/GLA ELISA Kit (Colorimetric) [NBP3-23444] - Samples were spiked with high concentrations of alpha-Galactosidase A/GLA and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human alpha-Galactosidase A/GLA ELISA Kit (Colorimetric) [NBP3-23444] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-23444_human-alpha-galactosidase-a-gla-elisa-kit-colorimetric-4320249141682.jpg)
![ELISA: Human alpha-Galactosidase A/GLA ELISA Kit (Colorimetric) [NBP2-82180] - Samples were spiked with high concentrations of Human alpha-Galactosidase A/GLA and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-82180_human-alpha-galactosidase-a-gla-elisa-kit-colorimetric-132202416143083.jpg "ELISA: Human alpha-Galactosidase A/GLA ELISA Kit (Colorimetric) [NBP2-82180] - Samples were spiked with high concentrations of Human alpha-Galactosidase A/GLA and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human alpha-Galactosidase A/GLA ELISA Kit (Colorimetric) [NBP2-82180] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-82180_human-alpha-galactosidase-a-gla-elisa-kit-colorimetric-432024963923.jpg)
![Western Blot: alpha-Galactosidase A/GLA Overexpression Lysate [NBL1-11104] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Galactosidase alpha.](http://images.novusbio.com/fullsize/Galactosidase-alpha-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11104-img0002.jpg "Western Blot: alpha-Galactosidase A/GLA Overexpression Lysate [NBL1-11104] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Galactosidase alpha.")
![Western Blot: alpha-Galactosidase A/GLA Antibody (201) [NBP2-90048] - Lane A: MCF7 Whole Cell LysateLane B: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:49 kDaObserved band size:49 kDa](http://images.novusbio.com/fullsize/alpha-Galactosidase-A-GLA-Antibody-201-Western-Blot-NBP2-90048-img0001.jpg "Western Blot: alpha-Galactosidase A/GLA Antibody (201) [NBP2-90048] - Lane A: MCF7 Whole Cell LysateLane B: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:49 kDaObserved band size:49 kDa")