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![Western Blot: GABA-A R gamma 2 Antibody [NB300-190] - Mouse whole brain lysate showing specific immunolabeling of the ~46 kDa gamma-2-subunit of GABAa -R.](http://images.novusbio.com/fullsize/GABA-A-R-gamma-2-Antibody-Western-Blot-NB300-190-img0002.jpg "Western Blot: GABA-A R gamma 2 Antibody [NB300-190] - Mouse whole brain lysate showing specific immunolabeling of the ~46 kDa gamma-2-subunit of GABAa -R.")
![Immunohistochemistry: GABA-A R gamma 2 Antibody [NB300-190] - Immunostaining of mouse retina showing specific labeling of the GABAa gamma-2 subunit in green, calbindin in red and DNA in blue. Photo courtesy of Dr. Arlene Hirano, UCLA.](http://images.novusbio.com/fullsize/GABA-A-R-gamma-2-Antibody-Immunocytochemistry-Immunofluorescence-NB300-190-img0001.jpg)
![Western Blot: GABA-A R gamma 2 Antibody [NB300-190] - Pattern of YFP gamma 2 expression in retinas of Thy1-YFP gamma 2 transgenic mice.(A) Maximum intensity projections (MIP) of confocal image stacks (2 µm total thickness) of vertical slices of Thy1-YFP gamma 2 retinas at postnatal day (P)12 & P21. Diffuse fluorescence expression in cell bodies within the ganglion cell layer (GCL) at both ages, & punctate expression throughout both OFF & ON layers of the inner plexiform layer (IPL) are apparent. There was no expression in the outer plexiform layer (OPL) or the outer nuclear layer (ONL) at either age. (B) Images of MIPs (∼20 µm thick) at various depths of a P21 flat mount Thy1-YFP gamma 2 retina. Inserts are 3× magnification of the images. (C) Western blot of whole brain lysates from Thy1-YFP gamma 2 (Tg) & wildtype (WT) mice. YFP gamma 2 (arrowhead) was detected with anti-GABAAR gamma 2 (upper) & anti-GFP (lower). Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0069612), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-190_rabbit-polyclonal-gaba-a-r-gamma-2-antibody-31020241620510.jpg)
![Immunocytochemistry/ Immunofluorescence: GABA-A R gamma 2 Antibody [NB300-190] - YFP gamma 2 fluorescence correlates with immunostaining for GABAAR alpha subunits.Cross-correlation analysis of vertical sections from P12 & P21 Thy1-YFP gamma 2 retinas stained with anti-GABAAR alpha 1 (A–D), GABAAR alpha 2 (E–H), or GABAAR alpha 3 (I–L). (M) Image stack of a single ON RGC labeled by transfection of CMV:tdTomato in a Thy1-YFP gamma 2 retina, immunostained for GABAAR alpha 3. (N) The dendritic label was used to mask out the YFP gamma 2 & GABAAR alpha 3 fluorescence. (O) (Upper) YFP gamma 2 & GABAAR alpha 3 puncta within sections of dendritic arbors identified by custom-written software. (Lower) Fraction of the population of YFP gamma 2 puncta that colocalized with GABAAR alpha 3, & vice-versa (average & SEM of 4 cells). White arrowheads are examples of colocalized puncta, green & red arrowheads, non-colocalized puncta. Scale bars 5 µm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0069612), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-190_rabbit-polyclonal-gaba-a-r-gamma-2-antibody-310202416212325.jpg)
![Immunocytochemistry/ Immunofluorescence: GABA-A R gamma 2 Antibody [NB300-190] - YFP gamma 2 fluorescence correlates with immunostaining for GABAAR alpha subunits.Cross-correlation analysis of vertical sections from P12 & P21 Thy1-YFP gamma 2 retinas stained with anti-GABAAR alpha 1 (A–D), GABAAR alpha 2 (E–H), or GABAAR alpha 3 (I–L). (M) Image stack of a single ON RGC labeled by transfection of CMV:tdTomato in a Thy1-YFP gamma 2 retina, immunostained for GABAAR alpha 3. (N) The dendritic label was used to mask out the YFP gamma 2 & GABAAR alpha 3 fluorescence. (O) (Upper) YFP gamma 2 & GABAAR alpha 3 puncta within sections of dendritic arbors identified by custom-written software. (Lower) Fraction of the population of YFP gamma 2 puncta that colocalized with GABAAR alpha 3, & vice-versa (average & SEM of 4 cells). White arrowheads are examples of colocalized puncta, green & red arrowheads, non-colocalized puncta. Scale bars 5 µm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0069612), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-190_rabbit-polyclonal-gaba-a-r-gamma-2-antibody-310202416212373.jpg)
![Immunocytochemistry/ Immunofluorescence: GABA-A R gamma 2 Antibody [NB300-190] - YFP gamma 2 fluorescence correlates with immunostaining for GABAAR alpha subunits.Cross-correlation analysis of vertical sections from P12 & P21 Thy1-YFP gamma 2 retinas stained with anti-GABAAR alpha 1 (A–D), GABAAR alpha 2 (E–H), or GABAAR alpha 3 (I–L). (M) Image stack of a single ON RGC labeled by transfection of CMV:tdTomato in a Thy1-YFP gamma 2 retina, immunostained for GABAAR alpha 3. (N) The dendritic label was used to mask out the YFP gamma 2 & GABAAR alpha 3 fluorescence. (O) (Upper) YFP gamma 2 & GABAAR alpha 3 puncta within sections of dendritic arbors identified by custom-written software. (Lower) Fraction of the population of YFP gamma 2 puncta that colocalized with GABAAR alpha 3, & vice-versa (average & SEM of 4 cells). White arrowheads are examples of colocalized puncta, green & red arrowheads, non-colocalized puncta. Scale bars 5 µm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0069612), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-190_rabbit-polyclonal-gaba-a-r-gamma-2-antibody-31020241621817.jpg)
![Immunocytochemistry/ Immunofluorescence: GABA-A R gamma 2 Antibody [NB300-190] - YFP gamma 2 fluorescence correlates with immunostaining for GABAAR alpha subunits.Cross-correlation analysis of vertical sections from P12 & P21 Thy1-YFP gamma 2 retinas stained with anti-GABAAR alpha 1 (A–D), GABAAR alpha 2 (E–H), or GABAAR alpha 3 (I–L). (M) Image stack of a single ON RGC labeled by transfection of CMV:tdTomato in a Thy1-YFP gamma 2 retina, immunostained for GABAAR alpha 3. (N) The dendritic label was used to mask out the YFP gamma 2 & GABAAR alpha 3 fluorescence. (O) (Upper) YFP gamma 2 & GABAAR alpha 3 puncta within sections of dendritic arbors identified by custom-written software. (Lower) Fraction of the population of YFP gamma 2 puncta that colocalized with GABAAR alpha 3, & vice-versa (average & SEM of 4 cells). White arrowheads are examples of colocalized puncta, green & red arrowheads, non-colocalized puncta. Scale bars 5 µm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0069612), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-190_rabbit-polyclonal-gaba-a-r-gamma-2-antibody-310202416212319.jpg)
![Immunocytochemistry/ Immunofluorescence: GABA-A R gamma 2 Antibody [NB300-190] - YFP gamma 2 fluorescence correlates with immunostaining for GABAAR alpha subunits.Cross-correlation analysis of vertical sections from P12 & P21 Thy1-YFP gamma 2 retinas stained with anti-GABAAR alpha 1 (A–D), GABAAR alpha 2 (E–H), or GABAAR alpha 3 (I–L). (M) Image stack of a single ON RGC labeled by transfection of CMV:tdTomato in a Thy1-YFP gamma 2 retina, immunostained for GABAAR alpha 3. (N) The dendritic label was used to mask out the YFP gamma 2 & GABAAR alpha 3 fluorescence. (O) (Upper) YFP gamma 2 & GABAAR alpha 3 puncta within sections of dendritic arbors identified by custom-written software. (Lower) Fraction of the population of YFP gamma 2 puncta that colocalized with GABAAR alpha 3, & vice-versa (average & SEM of 4 cells). White arrowheads are examples of colocalized puncta, green & red arrowheads, non-colocalized puncta. Scale bars 5 µm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0069612), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-190_rabbit-polyclonal-gaba-a-r-gamma-2-antibody-310202416205172.jpg)
![Immunocytochemistry/ Immunofluorescence: GABA-A R gamma 2 Antibody [NB300-190] - YFP gamma 2 fluorescence correlates with immunostaining for GABAAR alpha subunits.Cross-correlation analysis of vertical sections from P12 & P21 Thy1-YFP gamma 2 retinas stained with anti-GABAAR alpha 1 (A–D), GABAAR alpha 2 (E–H), or GABAAR alpha 3 (I–L). (M) Image stack of a single ON RGC labeled by transfection of CMV:tdTomato in a Thy1-YFP gamma 2 retina, immunostained for GABAAR alpha 3. (N) The dendritic label was used to mask out the YFP gamma 2 & GABAAR alpha 3 fluorescence. (O) (Upper) YFP gamma 2 & GABAAR alpha 3 puncta within sections of dendritic arbors identified by custom-written software. (Lower) Fraction of the population of YFP gamma 2 puncta that colocalized with GABAAR alpha 3, & vice-versa (average & SEM of 4 cells). White arrowheads are examples of colocalized puncta, green & red arrowheads, non-colocalized puncta. Scale bars 5 µm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0069612), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-190_rabbit-polyclonal-gaba-a-r-gamma-2-antibody-310202416205153.jpg)
![Immunocytochemistry/ Immunofluorescence: GABA-A R gamma 2 Antibody [NB300-190] - YFP gamma 2 fluorescence correlates with immunostaining for GABAAR alpha subunits.Cross-correlation analysis of vertical sections from P12 & P21 Thy1-YFP gamma 2 retinas stained with anti-GABAAR alpha 1 (A–D), GABAAR alpha 2 (E–H), or GABAAR alpha 3 (I–L). (M) Image stack of a single ON RGC labeled by transfection of CMV:tdTomato in a Thy1-YFP gamma 2 retina, immunostained for GABAAR alpha 3. (N) The dendritic label was used to mask out the YFP gamma 2 & GABAAR alpha 3 fluorescence. (O) (Upper) YFP gamma 2 & GABAAR alpha 3 puncta within sections of dendritic arbors identified by custom-written software. (Lower) Fraction of the population of YFP gamma 2 puncta that colocalized with GABAAR alpha 3, & vice-versa (average & SEM of 4 cells). White arrowheads are examples of colocalized puncta, green & red arrowheads, non-colocalized puncta. Scale bars 5 µm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0069612), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-190_rabbit-polyclonal-gaba-a-r-gamma-2-antibody-310202416212384.jpg)
![Immunocytochemistry/ Immunofluorescence: GABA-A R gamma 2 Antibody [NB300-190] - YFP gamma 2 fluorescence correlates with immunostaining for GABAAR alpha subunits.Cross-correlation analysis of vertical sections from P12 & P21 Thy1-YFP gamma 2 retinas stained with anti-GABAAR alpha 1 (A–D), GABAAR alpha 2 (E–H), or GABAAR alpha 3 (I–L). (M) Image stack of a single ON RGC labeled by transfection of CMV:tdTomato in a Thy1-YFP gamma 2 retina, immunostained for GABAAR alpha 3. (N) The dendritic label was used to mask out the YFP gamma 2 & GABAAR alpha 3 fluorescence. (O) (Upper) YFP gamma 2 & GABAAR alpha 3 puncta within sections of dendritic arbors identified by custom-written software. (Lower) Fraction of the population of YFP gamma 2 puncta that colocalized with GABAAR alpha 3, & vice-versa (average & SEM of 4 cells). White arrowheads are examples of colocalized puncta, green & red arrowheads, non-colocalized puncta. Scale bars 5 µm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0069612), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-190_rabbit-polyclonal-gaba-a-r-gamma-2-antibody-310202416212327.jpg)
![Western Blot: GABA-A R gamma 2 Antibody [NB300-151] - 10 ug of rat brain lysate showing immunolabeling of the ~45-47 kDa GABA - A R, g2-subunit.](http://images.novusbio.com/fullsize/GABA-A-R-gamma-2-Antibody-Western-Blot-NB300-151-img0007.jpg "Western Blot: GABA-A R gamma 2 Antibody [NB300-151] - 10 ug of rat brain lysate showing immunolabeling of the ~45-47 kDa GABA - A R, g2-subunit.")
![Immunohistochemistry-Paraffin: GABA-A R gamma 2 Antibody [NB300-151] - Rat cerebellum.](http://images.novusbio.com/fullsize/GABA-A-R-gamma-2-Antibody-Immunohistochemistry-Paraffin-NB300-151-img0005.jpg)
![Western Blot: GABA-A R gamma 2 Antibody [NB300-151] - 10 ug of rat hippocampal lysate showing immunolabeling of the ~46k gamma2-subunit of the GABA-A R.](http://images.novusbio.com/fullsize/GABA-A-R-gamma-2-Antibody-Western-Blot-NB300-151-img0006.jpg)
![Western Blot: GABA-A R gamma 2 Antibody [NBP1-49868] - Analysis of GABA -A Receptor gamma 2 in Mouse Brain lysate (35ug protein in RIPA buffer) using NBP1-49868 at 2 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/GABA-A-R-gamma-2-Antibody-Western-Blot-NBP1-49868-img0003.jpg "Western Blot: GABA-A R gamma 2 Antibody [NBP1-49868] - Analysis of GABA -A Receptor gamma 2 in Mouse Brain lysate (35ug protein in RIPA buffer) using NBP1-49868 at 2 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: GABA-A R gamma 2 [p Ser327] Antibody [NBP1-28661] - Western blot of rat cortex showing specific immunolabeling of the ~45k GABA-A R gamma 2 protein phosphorylated at Ser327 (control). Immunolabeling is blocked by the phospho-peptide (peptide) used as antigen but not by the corresponding dephosphopeptide (not shown).](http://images.novusbio.com/fullsize/GABA-A-R-gamma-2-[p-Ser327]-Antibody-Western-Blot-NBP1-28661-img0002.jpg "Western Blot: GABA-A R gamma 2 [p Ser327] Antibody [NBP1-28661] - Western blot of rat cortex showing specific immunolabeling of the ~45k GABA-A R gamma 2 protein phosphorylated at Ser327 (control). Immunolabeling is blocked by the phospho-peptide (peptide) used as antigen but not by the corresponding dephosphopeptide (not shown).")
![Western Blot: GABA A Receptor gamma 2 Overexpression Lysate (Adult Normal) [NBL1-10926] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GABA A Receptor gamma 2.](http://images.novusbio.com/fullsize/GABA-A-Receptor-gamma-2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-10926-img0002.jpg "Western Blot: GABA A Receptor gamma 2 Overexpression Lysate (Adult Normal) [NBL1-10926] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GABA A Receptor gamma 2.")