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![Western Blot: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOSL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOSL1.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-12F9-Western-Blot-NBP1-47757-img0019.jpg "Western Blot: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOSL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOSL1.")
![Immunohistochemistry-Paraffin: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - Carcinoma of bladder tissue.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-12F9-Immunohistochemistry-Paraffin-NBP1-47757-img0020.jpg)
![Immunohistochemistry-Paraffin: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-12F9-Immunohistochemistry-Paraffin-NBP1-47757-img0011.jpg)
![Immunohistochemistry-Paraffin: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-12F9-Immunohistochemistry-Paraffin-NBP1-47757-img0012.jpg)
![Immunohistochemistry-Paraffin: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-12F9-Immunohistochemistry-Paraffin-NBP1-47757-img0013.jpg)
![Immunohistochemistry-Paraffin: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - Staining of paraffin-embedded colon tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-12F9-Immunohistochemistry-Paraffin-NBP1-47757-img0014.jpg)
![Immunohistochemistry-Paraffin: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - Staining of paraffin-embedded endometrium tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-12F9-Immunohistochemistry-Paraffin-NBP1-47757-img0015.jpg)
![Immunohistochemistry-Paraffin: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - Staining of paraffin-embedded Kidney tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-12F9-Immunohistochemistry-Paraffin-NBP1-47757-img0016.jpg)
![Immunohistochemistry-Paraffin: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - Staining of paraffin-embedded Ovary tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-12F9-Immunohistochemistry-Paraffin-NBP1-47757-img0017.jpg)
![Immunohistochemistry-Paraffin: FRA-1/FOSL1 Antibody (12F9) [NBP1-47757] - Staining of paraffin-embedded prostate tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-12F9-Immunohistochemistry-Paraffin-NBP1-47757-img0018.jpg)
![Western Blot: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOSL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOSL1.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Western-Blot-NBP2-71351-img0001.jpg "Western Blot: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOSL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOSL1.")
![Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - Staining of paraffin-embedded Ovary tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71351-img0009.jpg)
![Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - Carcinoma of bladder tissue.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71351-img0002.jpg)
![Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71351-img0003.jpg)
![Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71351-img0004.jpg)
![Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - Staining of paraffin-embedded colon tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71351-img0005.jpg)
![Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - Staining of paraffin-embedded endometrium tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71351-img0006.jpg)
![Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - Staining of paraffin-embedded Kidney tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71351-img0007.jpg)
![Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71351-img0008.jpg)
![Immunohistochemistry: FRA-1/FOSL1 Antibody (OTI12F9) - Azide and BSA Free [NBP2-71351] - Staining of paraffin-embedded prostate tissue using anti-FOSL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-OTI12F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71351-img0010.jpg)
![Western Blot: FRA-1/FOSL1 Antibody (JE33-20) [NBP3-32350] - Western blot analysis of FRA-1/FOSL1 on different lysates with Rabbit anti-FRA-1/FOSL1 antibody (NBP3-32350) at 1/1,000 dilution. Lane 1: MDA-MB-231 cell lysate Lane 2: U-87 MG cell lysate Lane 3: A431 cell lysate Lane 4: HeLa cell lysate (negative) Lane 5: HEK-293 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 29 kDa Observed band size: 37 kDa (40 kDa unknown) Exposure time: 35 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32350) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32350_rabbit-fra-1-fosl1-mab-je33-20-278202416331227.jpg "Western Blot: FRA-1/FOSL1 Antibody (JE33-20) [NBP3-32350] - Western blot analysis of FRA-1/FOSL1 on different lysates with Rabbit anti-FRA-1/FOSL1 antibody (NBP3-32350) at 1/1,000 dilution. Lane 1: MDA-MB-231 cell lysate Lane 2: U-87 MG cell lysate Lane 3: A431 cell lysate Lane 4: HeLa cell lysate (negative) Lane 5: HEK-293 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 29 kDa Observed band size: 37 kDa (40 kDa unknown) Exposure time: 35 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32350) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: FRA-1/FOSL1 Antibody (JE33-20) [NBP3-32350] - Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue with Rabbit anti-FRA-1/FOSL1 antibody (NBP3-32350) at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32350) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32350_rabbit-fra-1-fosl1-mab-je33-20-29820241435263.jpg)
![Immunocytochemistry/ Immunofluorescence: FRA-1/FOSL1 Antibody (JE33-20) [NBP3-32350] - Immunocytochemistry analysis of U-87 MG cells labeling FRA-1/FOSL1 with Rabbit anti-FRA-1/FOSL1 antibody (NBP3-32350) at 1/100 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-FRA-1/FOSL1 antibody (NBP3-32350) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32350_rabbit-fra-1-fosl1-mab-je33-20-29202410484854.jpg)
![Flow Cytometry: FRA-1/FOSL1 Antibody (JE33-20) [NBP3-32350] - Flow cytometric analysis of U-87 MG cells labeling FRA-1/FOSL1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32350, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32350_rabbit-fra-1-fosl1-mab-je33-20-29202416453260.jpg)
![Western Blot: FRA-1/FOSL1 Antibody [NBP3-13376] - NIH-3T3 whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with FRA-1/FOSL1 antibody (NBP3-13376) diluted at 1:2000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-Western-Blot-NBP3-13376-img0001.jpg "<b>Independent Antibodies Validation. </b>Western Blot: FRA-1/FOSL1 Antibody [NBP3-13376] - NIH-3T3 whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with FRA-1/FOSL1 antibody (NBP3-13376) diluted at 1:2000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Independent Antibodies Validation. </b>Western Blot: FRA-1/FOSL1 Antibody [NBP3-13376] - Untreated (-) and treated (+) Rat-2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with FRA-1/FOSL1 antibody (NBP3-13376) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13376_rabbit-fra-1-fosl1-pab-91020242014676.jpg)
![Western Blot: FRA-1/FOSL1 Antibody [NBP3-13376] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with FRA-1/FOSL1 antibody (NBP3-13376) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13376_rabbit-fra-1-fosl1-pab-9102024195729.jpg)
![Western Blot: FRA-1/FOSL1 Antibody [NB100-40803] - (1ug/ml) staining of Human Bone Marrow lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-Western-Blot-NB100-40803-img0003.jpg "Western Blot: FRA-1/FOSL1 Antibody [NB100-40803] - (1ug/ml) staining of Human Bone Marrow lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: FRA-1/FOSL1 Antibody (SR1652) [NBP3-22276] - Western blot analysis using NBP3-22276 in HL60 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22276_rabbit-fra-1-fosl1-mab-sr1652-6720239533738.jpg "Western Blot: FRA-1/FOSL1 Antibody (SR1652) [NBP3-22276] - Western blot analysis using NBP3-22276 in HL60 cell lysate.")
![Immunocytochemistry/Immunofluorescence: FRA-1/FOSL1 Antibody [NBP3-17851] - Staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-17851-img0001.jpg "Immunocytochemistry/Immunofluorescence: FRA-1/FOSL1 Antibody [NBP3-17851] - Staining of human cell line U-2 OS shows localization to nucleoplasm.")
![Immunocytochemistry/Immunofluorescence: FRA-1/FOSL1 Antibody [NBP2-57326] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/FRA-1-FOSL1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57326-img0001.jpg "Immunocytochemistry/Immunofluorescence: FRA-1/FOSL1 Antibody [NBP2-57326] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.")