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![Immunohistochemistry-Paraffin: FANCI Antibody [NBP1-84608] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.](http://images.novusbio.com/fullsize/FANCI-Antibody-Immunohistochemistry-Paraffin-NBP1-84608-img0009.jpg "Immunohistochemistry-Paraffin: FANCI Antibody [NBP1-84608] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.")
![Immunohistochemistry-Paraffin: FANCI Antibody [NBP1-84608] - Staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.](http://images.novusbio.com/fullsize/FANCI-Antibody-Immunohistochemistry-Paraffin-NBP1-84608-img0005.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FANCI Antibody [NBP1-84608] - Analysis in human testis and liver tissues using NBP1-84608 antibody. Corresponding FANCI RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/FANCI-Antibody-Immunohistochemistry-Paraffin-NBP1-84608-img0006.jpg)
![Immunohistochemistry-Paraffin: FANCI Antibody [NBP1-84608] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/FANCI-Antibody-Immunohistochemistry-Paraffin-NBP1-84608-img0007.jpg)
![Immunohistochemistry-Paraffin: FANCI Antibody [NBP1-84608] - Staining of human Rectum shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/FANCI-Antibody-Immunohistochemistry-Paraffin-NBP1-84608-img0008.jpg)
![Immunohistochemistry-Paraffin: FANCI Antibody [NBP1-84608] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/FANCI-Antibody-Immunohistochemistry-Paraffin-NBP1-84608-img0010.jpg)
![Western Blot: FANCI Antibody [NB100-60447] - Detection of human FANCI by western blot. Samples: Whole cell lysate (50 ug) from HEK293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FANCI antibody NB100-60447 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/FANCI-Antibody-Western-Blot-NB100-60447-img0005.jpg "Western Blot: FANCI Antibody [NB100-60447] - Detection of human FANCI by western blot. Samples: Whole cell lysate (50 ug) from HEK293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FANCI antibody NB100-60447 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: FANCI Antibody [NB100-60447] - Detection of human FANCI by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FANCI antibody NB100-60447 used for IP at 3 ug per reaction. FANCI was also immunoprecipitated by rabbit anti-FANCI antibody from Company B. For blotting immunoprecipitated FANCI, NB100-60447 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/FANCI-Antibody-Immunoprecipitation-NB100-60447-img0006.jpg)
![Western Blot: FANCI Antibody [NB300-243] - Detection of Human FANCI by Western Blot. Samples : Nuclear extract (5 and 20 ug) from HeLa cells. Antibody : Affinity purified rabbit anti-FANCI antibody used at 0.2 ug/ml. Detection : Chemiluminescence with an exposure time of 15 minutes.](http://images.novusbio.com/fullsize/FANCI-Antibody-Western-Blot-NB300-243-img0003.jpg "Western Blot: FANCI Antibody [NB300-243] - Detection of Human FANCI by Western Blot. Samples : Nuclear extract (5 and 20 ug) from HeLa cells. Antibody : Affinity purified rabbit anti-FANCI antibody used at 0.2 ug/ml. Detection : Chemiluminescence with an exposure time of 15 minutes.")
![Western Blot: FANCI [p Ser559] Antibody [NBP2-60781] - Western blot of Hela cell lysate treated with UV (~254nm) for20' showing specific immunolabeling of the ~150 kDa FANCIprotein phosphorylated at Ser559 in the first lane (-).Phosphospecificity is shown in the second lane (+) whereimmunolabeling is completely eliminated by blot treatment withlambda phosphatase (Lambda-Ptase, 1200 units for 30 min).](http://images.novusbio.com/fullsize/FANCI-[p-Ser559]-Antibody-Western-Blot-NBP2-60781-img0003.jpg "Western Blot: FANCI [p Ser559] Antibody [NBP2-60781] - Western blot of Hela cell lysate treated with UV (~254nm) for20' showing specific immunolabeling of the ~150 kDa FANCIprotein phosphorylated at Ser559 in the first lane (-).Phosphospecificity is shown in the second lane (+) whereimmunolabeling is completely eliminated by blot treatment withlambda phosphatase (Lambda-Ptase, 1200 units for 30 min).")
![Western Blot: FANCI [p Ser559] Antibody [NBP2-60781] - Western blot of HeLa cell lysates that had been treated withUV (~254 nm) for 0' or 20' showing specific immunolabeling of the~150 kDa FANCI protein phosphorylated at Ser559.](http://images.novusbio.com/fullsize/FANCI-[p-Ser559]-Antibody-Western-Blot-NBP2-60781-img0001.jpg)
![Western Blot: FANCI [p Ser556] Antibody [NBP2-60780] - Western blot of Hela cell lysate treated with UV (~254nm) for20' showing specific immunolabeling of the ~150 kDa FANCIprotein phosphorylated at Ser559 in the first lane (-).Phosphospecificity is shown in the second lane (+) whereimmunolabeling is completely eliminated by blot treatment withlambda phosphatase (Lambda-Ptase, 1200 units for 30 min).](http://images.novusbio.com/fullsize/FANCI-[p-Ser556]-Antibody-Western-Blot-NBP2-60780-img0004.jpg "Western Blot: FANCI [p Ser556] Antibody [NBP2-60780] - Western blot of Hela cell lysate treated with UV (~254nm) for20' showing specific immunolabeling of the ~150 kDa FANCIprotein phosphorylated at Ser559 in the first lane (-).Phosphospecificity is shown in the second lane (+) whereimmunolabeling is completely eliminated by blot treatment withlambda phosphatase (Lambda-Ptase, 1200 units for 30 min).")
![Western Blot: FANCI [p Ser556] Antibody [NBP2-60780] - Western blot of HeLa cell lysates that had been treated withUV (~254 nm) for 0' or 20' showing specific immunolabeling of the~150 kDa FANCI protein phosphorylated at Ser559.](http://images.novusbio.com/fullsize/FANCI-[p-Ser556]-Antibody-Western-Blot-NBP2-60780-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: FANCI Antibody [NBP2-55040] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/FANCI-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55040-img0001.jpg "Immunocytochemistry/Immunofluorescence: FANCI Antibody [NBP2-55040] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.")