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![Western Blot: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Western blot analysis of E2F-1 on NIH/3T3 cell lysates with Rabbit anti-E2F1 antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 47 kDa Observed band size: 60 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/E2F-1-Antibody-JJ092-02-Western-Blot-NBP2-67899-img0011.jpg "Western Blot: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Western blot analysis of E2F-1 on NIH/3T3 cell lysates with Rabbit anti-E2F1 antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 47 kDa Observed band size: 60 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Staining E2F1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/E2F-1-Antibody-JJ092-02-Immunocytochemistry-Immunofluorescence-NBP2-67899-img0004.jpg)
![Immunohistochemistry-Paraffin: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-E2F-1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/E2F-1-Antibody-JJ092-02-Immunohistochemistry-Paraffin-NBP2-67899-img0012.jpg)
![Flow Cytometry: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Analysis of Hela cells with E2F1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/E2F-1-Antibody-JJ092-02-Flow-Cytometry-NBP2-67899-img0001.jpg)
![Western Blot: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Analysis of E2F1 on HepG2 cells lysates using anti-E2F1 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/E2F-1-Antibody-JJ092-02-Western-Blot-NBP2-67899-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Staining E2F1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/E2F-1-Antibody-JJ092-02-Immunocytochemistry-Immunofluorescence-NBP2-67899-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Staining E2F1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/E2F-1-Antibody-JJ092-02-Immunocytochemistry-Immunofluorescence-NBP2-67899-img0003.jpg)
![Immunohistochemistry-Paraffin: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-E2F1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/E2F-1-Antibody-JJ092-02-Immunohistochemistry-Paraffin-NBP2-67899-img0005.jpg)
![Immunohistochemistry-Paraffin: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Analysis of paraffin-embedded mouse pancreas tissue using anti-E2F1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/E2F-1-Antibody-JJ092-02-Immunohistochemistry-Paraffin-NBP2-67899-img0006.jpg)
![Immunohistochemistry-Paraffin: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-E2F-1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/E2F-1-Antibody-JJ092-02-Immunohistochemistry-Paraffin-NBP2-67899-img0008.jpg)
![Immunohistochemistry-Paraffin: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-E2F-1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/E2F-1-Antibody-JJ092-02-Immunohistochemistry-Paraffin-NBP2-67899-img0009.jpg)
![Immunohistochemistry-Paraffin: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-E2F-1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/E2F-1-Antibody-JJ092-02-Immunohistochemistry-Paraffin-NBP2-67899-img0010.jpg)
![Western Blot: E2F-1 Antibody (16G7) [NB600-210] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with E2F1 antibody [16G7] diluted at 1:1000. The signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/E2F-1-Antibody-16G7-Western-Blot-NB600-210-img0011.jpg "Western Blot: E2F-1 Antibody (16G7) [NB600-210] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with E2F1 antibody [16G7] diluted at 1:1000. The signal was developed with Trident ECL plus-Enhanced.")
![Immunocytochemistry/Immunofluorescence: E2F-1 Antibody (16G7) [NB600-210] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: E2F1 protein stained by E2F1 antibody [16G7] diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:50.](http://images.novusbio.com/fullsize/E2F-1-Antibody-16G7-Immunocytochemistry-Immunofluorescence-NB600-210-img0010.jpg)
![Immunohistochemistry: E2F-1 Antibody (1D12) [NBP3-26609] - Image of E2F-1 Antibody (1D12) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26609_rabbit-e2f-1-mab-1d12-262202414471553.jpg "Immunohistochemistry: E2F-1 Antibody (1D12) [NBP3-26609] - Image of E2F-1 Antibody (1D12) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: E2F-1 Antibody (1D12) [NBP3-26609] - Image of E2F-1 Antibody (1D12) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26609_rabbit-e2f-1-mab-1d12-27220248471914.jpg)
![Western Blot: E2F-1 Antibody (1D12) [NBP3-26609] - Positive Western Blot detected in: Jurkat whole cell lysate, PC-3 whole cell lysate, Rat Heart tissue. All lanes: E2F-1 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 47 kDa Observed band size: 60 kDa](http://images.novusbio.com/fullsize/nbp3-26609_rabbit-e2f-1-mab-1d12-2822024115259.jpg)
![Immunocytochemistry/Immunofluorescence: E2F-1 Antibody (1D12) [NBP3-26609] - Staining of Hela Cells with E2F-1 Antibody (1D12) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26609_rabbit-e2f-1-mab-1d12-282202412493640.jpg)
![Flow Cytometry: E2F-1 Antibody (1D12) [NBP3-26609] - Overlay histogram showing Hela cells stained with E2F-1 Antibody (1D12) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26609_rabbit-e2f-1-mab-1d12-282202414312778.jpg)
![Western Blot: E2F1 Antibody (18E10) [NB600-209] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/E2F1-Antibody-18E10-Western-Blot-NB600-209-img0002.jpg "Western Blot: E2F1 Antibody (18E10) [NB600-209] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE; antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: E2F-1 Antibody (18E10) [NB600-209] - E2F-1 antibody [18E10] detects E2F-1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse ovary.E2F-1 stained by E2F-1 antibody [18E10] (NB600-209) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nb600-209_mouse-monoclonal-e2f-1-antibody-18e10-910202419593516.jpg)
![Western Blot: E2F-1 Antibody (S03-5G3) [NBP3-19622] - Western blot detection of E2F-1 in C6, 3T3 cell lysates using NBP3-19622 (1:1000 diluted). Predicted band size: 47kDa. Observed band size: 70kDa.](http://images.novusbio.com/fullsize/E2F-1-Antibody-S03-5G3-Western-Blot-NBP3-19622-img0003.jpg "Western Blot: E2F-1 Antibody (S03-5G3) [NBP3-19622] - Western blot detection of E2F-1 in C6, 3T3 cell lysates using NBP3-19622 (1:1000 diluted). Predicted band size: 47kDa. Observed band size: 70kDa.")
![Immunocytochemistry/Immunofluorescence: E2F-1 Antibody (S03-5G3) [NBP3-19622] - Immunofluorescence of E2F-1 (green) in Hela cells using NBP3-19622 at dilution 1/50, and DAPI (blue)](http://images.novusbio.com/fullsize/E2F-1-Antibody-S03-5G3-Immunocytochemistry-Immunofluorescence-NBP3-19622-img0001.jpg)
![Immunohistochemistry-Paraffin: E2F-1 Antibody (S03-5G3) [NBP3-19622] - Immunohistochemistry of E2F-1 in paraffin-embedded Human tonsil using NBP3-19622 at dilution 1/2](http://images.novusbio.com/fullsize/E2F-1-Antibody-S03-5G3-Immunohistochemistry-Paraffin-NBP3-19622-img0002.jpg)
![Western Blot: E2F-1 [p Ser364] Antibody [NB600-900] - Panel A: Control antibody reactive to all forms of E2F (arrowheads). Panel B: Reactivity with phosphorylated E2F-1 (arrows) using E2F-1 [p Ser364] antibody. Lysate cells: CRE/E2F-1 CRE (human E2F-1 transfected mouse NIH3T3); NIH-3T3 (-ve control), & MDA-MB-231. Each lysate prepared from untreated cells & cells with 2 uM Doxorubicin. MDA-MB-231 also treated with genistein. Same membrane probed with E2F-1 [p Ser364] antibody @ 1:250 dilution, then stripped & re-probed with pan E2F antibody (+ve control). +ve control antibody shows an E2F-1 band in all human cell lines, but not mouse cells. Doxorubicin increases expression of E2F-1 (Panel A). Images overlapped to confirm that specific anti-E2F-1 pS364 staining shown in treated human cells in Panel B specifically aligns with E2F-1 staining shown in Panel A. Blots can be processed with HRP conjugated Gt-a-Rabbit IgG MX10 for 45 min at RT for ECL detection.](http://images.novusbio.com/fullsize/E2F-1-[p-Ser364]-Antibody-Western-Blot-NB600-900-img0007.jpg "Western Blot: E2F-1 [p Ser364] Antibody [NB600-900] - Panel A: Control antibody reactive to all forms of E2F (arrowheads). Panel B: Reactivity with phosphorylated E2F-1 (arrows) using E2F-1 [p Ser364] antibody. Lysate cells: CRE/E2F-1 CRE (human E2F-1 transfected mouse NIH3T3); NIH-3T3 (-ve control), & MDA-MB-231. Each lysate prepared from untreated cells & cells with 2 uM Doxorubicin. MDA-MB-231 also treated with genistein. Same membrane probed with E2F-1 [p Ser364] antibody @ 1:250 dilution, then stripped & re-probed with pan E2F antibody (+ve control). +ve control antibody shows an E2F-1 band in all human cell lines, but not mouse cells. Doxorubicin increases expression of E2F-1 (Panel A). Images overlapped to confirm that specific anti-E2F-1 pS364 staining shown in treated human cells in Panel B specifically aligns with E2F-1 staining shown in Panel A. Blots can be processed with HRP conjugated Gt-a-Rabbit IgG MX10 for 45 min at RT for ECL detection.")
![Immunohistochemistry: E2F-1 [p Ser364] Antibody [NB600-900] - Affinity Purified E2F-1 [p Ser364] antibody was used at a 10 ug/ml to detect nuclear and occasionally cytoplasmic signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. Within the multi-tumor block, the antibody showed variable levels of nuclear staining between individual tumors, with some tumors showing strong staining. This image shows E2F-1 pS364 staining of human breast carcinoma. Tissue was formalin-fixed and paraffin embedded.](http://images.novusbio.com/fullsize/E2F-1-[p-Ser364]-Antibody-Immunohistochemistry-NB600-900-img0008.jpg)
![Western Blot: E2F-1 [p Ser364] Antibody [NB600-900] - NB600-900 Western blot - E2F1 (phospho S364) antibody](http://images.novusbio.com/fullsize/E2F-1-[p-Ser364]-Antibody-Western-Blot-NB600-900-img0001.jpg)
![Western Blot: E2F-1 Antibody [NBP2-56716] - Analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/E2F-1-Antibody-Western-Blot-NBP2-56716-img0002.jpg "Western Blot: E2F-1 Antibody [NBP2-56716] - Analysis in human cell line RT-4 and human cell line U-251 MG.")
![Immunocytochemistry/Immunofluorescence: E2F-1 Antibody [NBP2-56716] - Staining of human cell line RH-30 shows localization to centrosome.](http://images.novusbio.com/fullsize/E2F-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56716-img0001.jpg)
![Western Blot: E2F-1 Antibody (5Z5U3) [NBP3-15785] - Western blot analysis of extracts of rat spleen, using E2F-1 antibody (NBP3-15785) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](http://images.novusbio.com/fullsize/E2F-1-Antibody-5Z5U3-Western-Blot-NBP3-15785-img0002.jpg "Western Blot: E2F-1 Antibody (5Z5U3) [NBP3-15785] - Western blot analysis of extracts of rat spleen, using E2F-1 antibody (NBP3-15785) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
![Western Blot: E2F-1 Antibody (5Z5U3) [NBP3-15785] - Western blot analysis of extracts of various cell lines, using E2F-1 antibody (NBP3-15785) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/E2F-1-Antibody-5Z5U3-Western-Blot-NBP3-15785-img0001.jpg)
![Immunoprecipitation: E2F-1 Antibody (5Z5U3) [NBP3-15785] - Immunoprecipitation analysis of 300ug extracts of HT-29 cells using 3ug E2F-1 antibody (NBP3-15785). Western blot was performed from the immunoprecipitate using E2F-1 antibody (NBP3-15785) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/E2F-1-Antibody-5Z5U3-Immunoprecipitation-NBP3-15785-img0003.jpg)
![Proximity Ligation Assay: E2F-1 Antibody (2E10) [H00001869-M01] - Analysis of protein-protein interactions between CDK7 and E2F1. HeLa cells were stained with anti-CDK7 rabbit purified polyclonal 1:1200 and anti-E2F1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/E2F-1-Antibody-2E10-Proximity-Ligation-Assay-H00001869-M01-img0002.jpg "Proximity Ligation Assay: E2F-1 Antibody (2E10) [H00001869-M01] - Analysis of protein-protein interactions between CDK7 and E2F1. HeLa cells were stained with anti-CDK7 rabbit purified polyclonal 1:1200 and anti-E2F1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).")
![Proximity Ligation Assay: E2F-1 Antibody (2E10) [H00001869-M01] - Analysis of protein-protein interactions between CDK7 and E2F1. Huh7 cells were stained with anti-CDK7 rabbit purified polyclonal 1:1200 and anti-E2F1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/E2F-1-Antibody-2E10-Proximity-Ligation-Assay-H00001869-M01-img0003.jpg)
![Proximity Ligation Assay: E2F-1 Antibody (2E10) [H00001869-M01] - Analysis of protein-protein interactions between MYBL2 and E2F1. Mahlavu cells were stained with anti-MYBL2 rabbit purified polyclonal 1:1200 and anti-E2F1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/E2F-1-Antibody-2E10-Proximity-Ligation-Assay-H00001869-M01-img0004.jpg)
![ELISA: E2F-1 Antibody (2E10) [H00001869-M01] - E2F-1 Antibody (2E10) [H00001869-M01] - Detection limit for recombinant GST tagged E2F1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/E2F-1-Antibody-2E10-ELISA-H00001869-M01-img0006.jpg)
![Western Blot: E2F1 Overexpression Lysate (Adult Normal) [NBP2-07771] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for E2F1.](http://images.novusbio.com/fullsize/E2F1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07771-img0001.jpg "Western Blot: E2F1 Overexpression Lysate (Adult Normal) [NBP2-07771] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for E2F1.")