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![Western Blot: CLCN2 Antibody (3E1) [H00001181-M01] - Analysis of CLCN2 expression in transfected 293T cell line by CLCN2 monoclonal antibody (M01), clone 3E1.Lane 1: CLCN2 transfected lysate(96.8 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CLCN2-Antibody-3E1-Western-Blot-H00001181-M01-img0007.jpg "Western Blot: CLCN2 Antibody (3E1) [H00001181-M01] - Analysis of CLCN2 expression in transfected 293T cell line by CLCN2 monoclonal antibody (M01), clone 3E1.Lane 1: CLCN2 transfected lysate(96.8 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: CLCN2 Antibody (3E1) [H00001181-M01] - Analysis of CLCN2 over-expressed 293 cell line, cotransfected with CLCN2 Validated Chimera RNAi ( Cat # H00001181-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CLCN2 monoclonal antibody (M01), clone 3E1 (Cat # H00001181-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/CLCN2-Antibody-3E1-Western-Blot-H00001181-M01-img0004.jpg)
![Western Blot: CLCN2 Antibody (3E1) [H00001181-M01] - CLCN2 monoclonal antibody (M01), clone 3E1 Analysis of CLCN2 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/CLCN2-Antibody-3E1-Western-Blot-H00001181-M01-img0006.jpg)
![Sandwich ELISA: CLCN2 Antibody (3E1) [H00001181-M01] - Detection limit for recombinant GST tagged CLCN2 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CLCN2-Antibody-3E1-Sandwich-ELISA-H00001181-M01-img0005.jpg)
![Immunohistochemistry-Paraffin: CLCN2 Antibody [NBP1-85584] - Staining of human skin shows very weak positivity in squamous epithelial cells as expected.](http://images.novusbio.com/fullsize/CLCN2-Antibody-Immunohistochemistry-Paraffin-NBP1-85584-img0006.jpg "Immunohistochemistry-Paraffin: CLCN2 Antibody [NBP1-85584] - Staining of human skin shows very weak positivity in squamous epithelial cells as expected.")
![Immunohistochemistry-Paraffin: CLCN2 Antibody [NBP1-85584] - Staining of human adrenal gland shows moderate granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/CLCN2-Antibody-Immunohistochemistry-Paraffin-NBP1-85584-img0003.jpg)
![Immunohistochemistry-Paraffin: CLCN2 Antibody [NBP1-85584] - Staining of human duodenum shows moderate granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/CLCN2-Antibody-Immunohistochemistry-Paraffin-NBP1-85584-img0004.jpg)
![Immunohistochemistry-Paraffin: CLCN2 Antibody [NBP1-85584] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/CLCN2-Antibody-Immunohistochemistry-Paraffin-NBP1-85584-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: CLCN2 Antibody [NBP1-85585] - Staining of human cell line CACO-2 shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CLCN2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85585-img0005.jpg "Immunocytochemistry/Immunofluorescence: CLCN2 Antibody [NBP1-85585] - Staining of human cell line CACO-2 shows localization to cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: CLCN2 Antibody [NBP1-85585] - Staining of human testis shows strong cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/CLCN2-Antibody-Immunohistochemistry-Paraffin-NBP1-85585-img0006.jpg)
![Western Blot: CLCN2 Antibody [NBP1-52032] - Analysis of CLCN2 in HeLa lysate (35ug protein in RIPA buffer) using this antibody at 0.3 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/CLCN2-Antibody-Western-Blot-NBP1-52032-img0002.jpg "Western Blot: CLCN2 Antibody [NBP1-52032] - Analysis of CLCN2 in HeLa lysate (35ug protein in RIPA buffer) using this antibody at 0.3 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.")
![SDS-Page: Recombinant Human CLCN2 Protein [H00001181-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00001181-P01-1.jpg "SDS-Page: Recombinant Human CLCN2 Protein [H00001181-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: CLCN2 Antibody [NBP2-92467] - Analysis of extracts of various cell lines, using CLCN2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/CLCN2-Antibody-Western-Blot-NBP2-92467-img0001.jpg "Western Blot: CLCN2 Antibody [NBP2-92467] - Analysis of extracts of various cell lines, using CLCN2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.")
![Western Blot: CLCN2 Overexpression Lysate (Adult Normal) [NBL1-09233] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CLCN2.](http://images.novusbio.com/fullsize/CLCN2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09233-img0002.jpg "Western Blot: CLCN2 Overexpression Lysate (Adult Normal) [NBL1-09233] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CLCN2.")
![Immunocytochemistry/Immunofluorescence: CLCN2 Antibody [NBP2-97349] - Immunofluorescence staining of CLCN2 in CACO2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human CLCN2 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/CLCN2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-97349-img0001.jpg "Immunocytochemistry/Immunofluorescence: CLCN2 Antibody [NBP2-97349] - Immunofluorescence staining of CLCN2 in CACO2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human CLCN2 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.")