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![Immunohistochemistry-Paraffin: Cingulin Antibody [NBP1-89600] - Staining in human small intestine and lymph node tissues using anti-CGN antibody. Corresponding CGN RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Cingulin-Antibody-Immunohistochemistry-Paraffin-NBP1-89600-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Cingulin Antibody [NBP1-89600] - Staining in human small intestine and lymph node tissues using anti-CGN antibody. Corresponding CGN RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: Cingulin Antibody [NBP1-89600] - Analysis using Anti-CGN antibody NBP1-89600 (A) shows similar pattern to independent antibody NBP1-89602 (B).](http://images.novusbio.com/fullsize/Cingulin-Antibody-Western-Blot-NBP1-89600-img0013.jpg)
![Western Blot: Cingulin Antibody [NBP1-89600] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Cingulin-Antibody-Western-Blot-NBP1-89600-img0008.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Cingulin Antibody [NBP1-89600] - Analysis in human cell lines Caco-2 and HeLa using Anti-CGN antibody. Corresponding CGN RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Cingulin-Antibody-Western-Blot-NBP1-89600-img0012.jpg)
![Immunohistochemistry-Paraffin: Cingulin Antibody [NBP1-89600] - Staining of human lymph node shows low expression as expected.](http://images.novusbio.com/fullsize/Cingulin-Antibody-Immunohistochemistry-Paraffin-NBP1-89600-img0009.jpg)
![Immunohistochemistry-Paraffin: Cingulin Antibody [NBP1-89600] - Staining of human small intestine shows high expression.](http://images.novusbio.com/fullsize/Cingulin-Antibody-Immunohistochemistry-Paraffin-NBP1-89600-img0010.jpg)
![Immunohistochemistry-Paraffin: Cingulin Antibody [NBP1-89602] - Staining in human small intestine and lymph node tissues using anti-CGN antibody. Corresponding CGN RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Cingulin-Antibody-Immunohistochemistry-Paraffin-NBP1-89602-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Cingulin Antibody [NBP1-89602] - Staining in human small intestine and lymph node tissues using anti-CGN antibody. Corresponding CGN RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: Cingulin Antibody [NBP1-89602] - Analysis using Anti-CGN antibody NBP1-89602 (A) shows similar pattern to independent antibody NBP1-89600 (B).](http://images.novusbio.com/fullsize/Cingulin-Antibody-Western-Blot-NBP1-89602-img0011.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Cingulin Antibody [NBP1-89602] - Western blot analysis in human cell lines Caco-2 and HeLa. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Cingulin-Antibody-Western-Blot-NBP1-89602-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Cingulin Antibody [NBP1-89602] - Staining of human cell line A-431 shows localization to plasma membrane & cell junctions. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Cingulin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89602-img0006.jpg)
![Immunohistochemistry-Paraffin: Cingulin Antibody [NBP1-89602] - Staining of human lymph node shows low expression as expected.](http://images.novusbio.com/fullsize/Cingulin-Antibody-Immunohistochemistry-Paraffin-NBP1-89602-img0007.jpg)
![Immunohistochemistry-Paraffin: Cingulin Antibody [NBP1-89602] - Staining of human small intestine shows high expression.](http://images.novusbio.com/fullsize/Cingulin-Antibody-Immunohistochemistry-Paraffin-NBP1-89602-img0008.jpg)
![Western Blot: Cingulin Antibody [NBP2-15904] - Sample (30 ug of whole cell lysate) A: Juakat 7. 5% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/Cingulin-Antibody-Western-Blot-NBP2-15904-img0001.jpg "Western Blot: Cingulin Antibody [NBP2-15904] - Sample (30 ug of whole cell lysate) A: Juakat 7. 5% SDS PAGE gel, diluted at 1:500.")
![Immunohistochemistry-Paraffin: Cingulin Antibody [NBP2-15904] - Cytoplasm on Ovarian carcinoma; antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Cingulin-Antibody-Immunohistochemistry-Paraffin-NBP2-15904-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: Cingulin Antibody [NBP2-15904] - Cingulin antibody detects Cingulin protein at cell membrane by immunofluorescent analysis.Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Cingulin stained by Cingulin antibody (NBP2-15904) diluted at 1:500.](http://images.novusbio.com/fullsize/nbp2-15904_rabbit-polyclonal-cingulin-antibody-910202420131214.jpg)
![Western Blot: Cingulin Antibody [NBP2-15904] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Cingulin antibody (NBP2-15904) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15904_rabbit-polyclonal-cingulin-antibody-910202420104457.jpg)
![Immunohistochemistry-Paraffin: Cingulin Antibody [NBP1-89601] - Staining of human small intestine shows high expression.](http://images.novusbio.com/fullsize/Cingulin-Antibody-Immunohistochemistry-Paraffin-NBP1-89601-img0004.jpg "Immunohistochemistry-Paraffin: Cingulin Antibody [NBP1-89601] - Staining of human small intestine shows high expression.")
![Immunohistochemistry-Paraffin: Cingulin Antibody [NBP1-89601] - Staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Cingulin-Antibody-Immunohistochemistry-Paraffin-NBP1-89601-img0002.jpg)
![Immunohistochemistry-Paraffin: Cingulin Antibody [NBP1-89601] - Staining of human lymph node shows low expression as expected.](http://images.novusbio.com/fullsize/Cingulin-Antibody-Immunohistochemistry-Paraffin-NBP1-89601-img0003.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Cingulin Antibody [NBP1-89601] - Staining in human small intestine and lymph node tissues using anti-CGN antibody. Corresponding CGN RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Cingulin-Antibody-Immunohistochemistry-Paraffin-NBP1-89601-img0005.jpg)
![Western Blot: Cingulin Antibody [NBP2-92240] - Analysis of extracts of various cell lines, using Cingulin at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/Cingulin-Antibody-Western-Blot-NBP2-92240-img0001.jpg "Western Blot: Cingulin Antibody [NBP2-92240] - Analysis of extracts of various cell lines, using Cingulin at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.")
![Immunohistochemistry-Paraffin: Cingulin Antibody [NBP2-92240] - Immunohistochemistry of paraffin-embedded Human colon carcinoma using Cingulin Rabbit pAb (NBP2-92240) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Cingulin-Antibody-Immunohistochemistry-Paraffin-NBP2-92240-img0002.jpg)