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![Western Blot: CDC42 Antibody (JJ086-04) [NBP2-67895] - Western blot analysis of CDC42 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: HepG2 cell lysate Lane 3: Jurkat cell lysate](http://images.novusbio.com/fullsize/CDC42-Antibody-JJ086-04-Western-Blot-NBP2-67895-img0006.jpg "Western Blot: CDC42 Antibody (JJ086-04) [NBP2-67895] - Western blot analysis of CDC42 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: HepG2 cell lysate Lane 3: Jurkat cell lysate")
![Immunohistochemistry-Paraffin: CDC42 Antibody (JJ086-04) [NBP2-67895] - Analysis of paraffin-embedded mouse pancreas tissue using anti-CDC42 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CDC42-Antibody-JJ086-04-Immunohistochemistry-Paraffin-NBP2-67895-img0004.jpg)
![Flow Cytometry: CDC42 Antibody (JJ086-04) [NBP2-67895] - Analysis of Hela cells with CDC42 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/CDC42-Antibody-JJ086-04-Flow-Cytometry-NBP2-67895-img0001.jpg)
![Immunohistochemistry-Paraffin: CDC42 Antibody (JJ086-04) [NBP2-67895] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-CDC42 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CDC42-Antibody-JJ086-04-Immunohistochemistry-Paraffin-NBP2-67895-img0002.jpg)
![Immunohistochemistry-Paraffin: CDC42 Antibody (JJ086-04) [NBP2-67895] - Analysis of paraffin-embedded human pancreas tissue using anti-CDC42 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CDC42-Antibody-JJ086-04-Immunohistochemistry-Paraffin-NBP2-67895-img0003.jpg)
![Western Blot: CDC42 Antibody [NBP2-68888] - Analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/CDC42-Antibody-Western-Blot-NBP2-68888-img0001.jpg "Western Blot: CDC42 Antibody [NBP2-68888] - Analysis in human cell line RT-4 and human cell line U-251 MG.")
![Immunohistochemistry-Paraffin: CDC42 Antibody [NBP2-68888] - Staining of human spleen shows cytoplasmic positivity in red pulp.](http://images.novusbio.com/fullsize/CDC42-Antibody-Immunohistochemistry-NBP2-68888-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CDC42 Antibody (M152) [NBP3-23212] - Immunocytochemical labeling in rat PC12 cells grown for 4 days on poly-D-lysine-coated plates in the presence (200 ng/ml) or absence (Control) of Nerve Growth Factor (NGF). Anti-Cdc42 (NBP3-23212) was used at 1:50 dilution followed by labeling with donkey anti-mouse:Cy2.](http://images.novusbio.com/fullsize/nbp3-23212_mouse-cdc42-mab-m152-19920231759449.jpg "Immunocytochemistry/Immunofluorescence: CDC42 Antibody (M152) [NBP3-23212] - Immunocytochemical labeling in rat PC12 cells grown for 4 days on poly-D-lysine-coated plates in the presence (200 ng/ml) or absence (Control) of Nerve Growth Factor (NGF). Anti-Cdc42 (NBP3-23212) was used at 1:50 dilution followed by labeling with donkey anti-mouse:Cy2.")
![Western Blot: CDC42 Antibody (M152) [NBP3-23212] - Western blot analysis of human jurkat cells (lanes 1 & 2) and mouse brain (lanes 3 & 4). The blots were probed with anti-Cdc42 antibody at 1:125 (lanes 1 & 3) or 1:500 (lanes 2 & 4).](http://images.novusbio.com/fullsize/nbp3-23212_mouse-cdc42-mab-m152-19920231410126.jpg)
![Immunohistochemistry: CDC42 Antibody (3C3) [NBP3-26670] - Image of CDC42 Antibody (3C3) diluted at 1:100 and staining in paraffin-embedded human pancreatic cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26670_rabbit-cdc42-mab-3c3-26220241447156.jpg "Immunohistochemistry: CDC42 Antibody (3C3) [NBP3-26670] - Image of CDC42 Antibody (3C3) diluted at 1:100 and staining in paraffin-embedded human pancreatic cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: CDC42 Antibody (3C3) [NBP3-26670] - Positive Western Blot detected in: NIH/3T3 whole cell lysate, K562 whole cell lysate, Mouse Brain whole cell lysate. All lanes: CDC42 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 22, 22 kDa Observed band size: 24 kDa](http://images.novusbio.com/fullsize/nbp3-26670_rabbit-cdc42-mab-3c3-2822024115256.jpg)
![Immunocytochemistry/Immunofluorescence: CDC42 Antibody (M430) [NBP3-23213] - Immunocytochemical labeling in paraformaldehyde fixed and NP-40 permeabilized rat A7r5 cells. The cells were labeled with mouse monoclonal Anti-Cdc42 (NBP3-23213) in the absence (Left) or presence of full length Cdc42 recombinant protein (Right), then the antibody was detected using Goat anti-Mouse secondary antibody conjugated to DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23213_mouse-cdc42-mab-m430-18920231735524.jpg "Immunocytochemistry/Immunofluorescence: CDC42 Antibody (M430) [NBP3-23213] - Immunocytochemical labeling in paraformaldehyde fixed and NP-40 permeabilized rat A7r5 cells. The cells were labeled with mouse monoclonal Anti-Cdc42 (NBP3-23213) in the absence (Left) or presence of full length Cdc42 recombinant protein (Right), then the antibody was detected using Goat anti-Mouse secondary antibody conjugated to DyLight(R) 594.")
![Western Blot: CDC42 Antibody [NBP1-76330] - Human brain tissue lysate with CDC42 antibody at (A) 0.5 and (B) 1 ug/ml.](http://images.novusbio.com/fullsize/CDC42-Antibody-Western-Blot-NBP1-76330-img0002.jpg "Western Blot: CDC42 Antibody [NBP1-76330] - Human brain tissue lysate with CDC42 antibody at (A) 0.5 and (B) 1 ug/ml.")
![Western Blot: CDC42 Antibody [NBP2-98725] - Anti-CDC42 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 21 kDa. Observed band size: 21 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/CDC42-Antibody-Western-Blot-NBP2-98725-img0004.jpg "Western Blot: CDC42 Antibody [NBP2-98725] - Anti-CDC42 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 21 kDa. Observed band size: 21 kDa (We are unsure as to the identity of these extra bands).")
![Immunoprecipitation: CDC42 Antibody [NBP2-98725] - CDC42 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 2 ul anti-CDC42 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-CDC42 rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odyssey technique. Performed under reducing conditions. Predicted band size: 21 kDa. Observed band size: 21 kDa](http://images.novusbio.com/fullsize/CDC42-Antibody-Immunoprecipitation-NBP2-98725-img0003.jpg)
![Western Blot: CDC42 Antibody (1V6X7) [NBP3-15619] - Western blot analysis of extracts of various cell lines, using CDC42 antibody (NBP3-15619) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/CDC42-Antibody-1V6X7-Western-Blot-NBP3-15619-img0001.jpg "Western Blot: CDC42 Antibody (1V6X7) [NBP3-15619] - Western blot analysis of extracts of various cell lines, using CDC42 antibody (NBP3-15619) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: CDC42 Overexpression Lysate (Adult Normal) [NBL1-09004] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CDC42.](http://images.novusbio.com/fullsize/CDC42-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09004-img0002.jpg "Western Blot: CDC42 Overexpression Lysate (Adult Normal) [NBL1-09004] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CDC42.")
![Western Blot: CDC42 Overexpression Lysate (Adult Normal) [NBL1-09005] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CDC42.](http://images.novusbio.com/fullsize/CDC42-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09005-img0002.jpg "Western Blot: CDC42 Overexpression Lysate (Adult Normal) [NBL1-09005] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CDC42.")
![Western Blot: CDC42 Overexpression Lysate (Adult Normal) [NBP2-05863] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CDC42.](http://images.novusbio.com/fullsize/CDC42-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-05863-img0001.jpg "Western Blot: CDC42 Overexpression Lysate (Adult Normal) [NBP2-05863] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CDC42.")
![Western Blot: CDC42 [p Ser71] Antibody (4X7Z2) [NBP3-16915] - Western blot analysis of extracts of A-431 cells, using CDC42 Rabbit mAb (NBP3-16915) at 1:1000 dilution.A-431 cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/CDC42-p-Ser71-Antibody-4X7Z2-Western-Blot-NBP3-16915-img0001.jpg "Western Blot: CDC42 [p Ser71] Antibody (4X7Z2) [NBP3-16915] - Western blot analysis of extracts of A-431 cells, using CDC42 Rabbit mAb (NBP3-16915) at 1:1000 dilution.A-431 cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 3min.")