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![Western Blot: CCT3 Antibody [NBP1-86562] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-CCT3 antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize/CCT3-Antibody-Western-Blot-NBP1-86562-img0012.jpg "<b>Genetic Strategies Validation. </b>Western Blot: CCT3 Antibody [NBP1-86562] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-CCT3 antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CCT3 Antibody [NBP1-86562] - Staining of human cell line U-2 OS shows localization to plasma membrane and cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CCT3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86562-img0011.jpg)
![Immunohistochemistry-Paraffin: CCT3 Antibody [NBP1-86562] - Staining of human prostate shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/CCT3-Antibody-Immunohistochemistry-Paraffin-NBP1-86562-img0010.jpg)
![Western Blot: CCT3 Antibody [NBP1-86562] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/CCT3-Antibody-Western-Blot-NBP1-86562-img0008.jpg)
![Western Blot: CCT3 Antibody [NBP1-79041] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), Jurkat (J; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-CCT3 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. CCT3 was also immunoprecipitated by rabbit anti-CCT3 antibody NBP1-79040 which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/CCT3-Antibody-Western-Blot-NBP1-79041-img0001.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: CCT3 Antibody [NBP1-79041] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), Jurkat (J; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-CCT3 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. CCT3 was also immunoprecipitated by rabbit anti-CCT3 antibody NBP1-79040 which recognizes an upstream epitope.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: CCT3 Antibody [NBP1-79041] - Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- CCT3 used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).](http://images.novusbio.com/fullsize/CCT3-Antibody-Immunohistochemistry-NBP1-79041-img0009.jpg)
![Immunohistochemistry-Paraffin: CCT3 Antibody [NBP1-79041] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti- CCT3 used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).](http://images.novusbio.com/fullsize/CCT3-Antibody-Immunohistochemistry-NBP1-79041-img0008.jpg)
![Western Blot: CCT3 Antibody [NBP1-79040] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), Jurkat (J; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-CCT3 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. CCT3 was also immunoprecipitated by rabbit anti-CCT3 antibody NBP1-79041 which recognizes a downstream epitope](http://images.novusbio.com/fullsize/CCT3-Antibody-Western-Blot-NBP1-79040-img0001.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: CCT3 Antibody [NBP1-79040] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), Jurkat (J; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-CCT3 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. CCT3 was also immunoprecipitated by rabbit anti-CCT3 antibody NBP1-79041 which recognizes a downstream epitope")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: CCT3 Antibody [NBP1-79040] - Section of mouse teratoma. Antibody: Affinity purified rabbit anti- CCT3 used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).](http://images.novusbio.com/fullsize/CCT3-Antibody-Immunohistochemistry-NBP1-79040-img0007.jpg)
![Immunohistochemistry-Paraffin: CCT3 Antibody [NBP1-79040] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti- CCT3 used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).](http://images.novusbio.com/fullsize/CCT3-Antibody-Immunohistochemistry-NBP1-79040-img0006.jpg)
![Western Blot: CCT3 Antibody [NBP2-92903] - Analysis of extracts of various cell lines, using CCT3 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.](http://images.novusbio.com/fullsize/CCT3-Antibody-Western-Blot-NBP2-92903-img0003.jpg "Western Blot: CCT3 Antibody [NBP2-92903] - Analysis of extracts of various cell lines, using CCT3 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.")
![Immunocytochemistry/Immunofluorescence: CCT3 Antibody [NBP2-92903] - Analysis of U-2 OS cells using CCT3 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CCT3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92903-img0001.jpg)
![Western Blot: CCT3 Antibody [NBP2-99266] - Anti-CCT3 rabbit polyclonal antibody at 1:500 dilution. Lane A: K562 Whole Cell Lysate Lane B: HL-60 Whole Cell Lysate Lane C: HeLa Whole Cell Lysate Lane D: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 61 kDa. Observed band size: 61 kDa](http://images.novusbio.com/fullsize/CCT3-Antibody-Western-Blot-NBP2-99266-img0002.jpg "Western Blot: CCT3 Antibody [NBP2-99266] - Anti-CCT3 rabbit polyclonal antibody at 1:500 dilution. Lane A: K562 Whole Cell Lysate Lane B: HL-60 Whole Cell Lysate Lane C: HeLa Whole Cell Lysate Lane D: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 61 kDa. Observed band size: 61 kDa")
![Immunocytochemistry/Immunofluorescence: CCT3 Antibody [NBP2-99266] - Immunofluorescence staining of CCT3 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human CCT3 polyclonal antibody (dilution ratio 1:500) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/CCT3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-99266-img0001.jpg)
![Western Blot: CCT3 Antibody [NBP2-99267] - Anti-CCT3 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 61 kDa. Observed band size: 61 kDa](http://images.novusbio.com/fullsize/CCT3-Antibody-Western-Blot-NBP2-99267-img0001.jpg "Western Blot: CCT3 Antibody [NBP2-99267] - Anti-CCT3 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 61 kDa. Observed band size: 61 kDa")
![Immunohistochemistry-Paraffin: CCT3 Antibody [NBP2-99267] - Immunochemical staining of human CCT3 in human prostate with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/CCT3-Antibody-Immunohistochemistry-Paraffin-NBP2-99267-img0003.jpg)
![Immunohistochemistry-Paraffin: CCT3 Antibody [NBP2-99267] - Immunochemical staining of human CCT3 in human colon carcinoma with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/CCT3-Antibody-Immunohistochemistry-Paraffin-NBP2-99267-img0002.jpg)
![Western Blot: CCT3 Antibody [NBP1-51949] - Analysis of CCT3 in Mouse Testis lysate (35ug protein in RIPA buffer) using this antibody at 0.01 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/CCT3-Antibody-Western-Blot-NBP1-51949-img0002.jpg "Western Blot: CCT3 Antibody [NBP1-51949] - Analysis of CCT3 in Mouse Testis lysate (35ug protein in RIPA buffer) using this antibody at 0.01 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: CCT3 Overexpression Lysate (Adult Normal) [NBL1-08899] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CCT3.](http://images.novusbio.com/fullsize/CCT3-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-08899-img0002.jpg "Western Blot: CCT3 Overexpression Lysate (Adult Normal) [NBL1-08899] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CCT3.")