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![Immunohistochemistry-Paraffin: MCAF1 Antibody [NBP1-84610] - Analysis in human testis and skeletal muscle tissues. Corresponding MCAF1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/MCAF1-Antibody-Immunohistochemistry-Paraffin-NBP1-84610-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: MCAF1 Antibody [NBP1-84610] - Analysis in human testis and skeletal muscle tissues. Corresponding MCAF1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: MCAF1 Antibody [NBP1-84610] - Staining of human cell line A-431 shows positivity in nuclei but not nucleoli. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/MCAF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84610-img0003.jpg)
![Immunohistochemistry-Paraffin: MCAF1 Antibody [NBP1-84610] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/MCAF1-Antibody-Immunohistochemistry-Paraffin-NBP1-84610-img0012.jpg)
![Immunohistochemistry-Paraffin: MCAF1 Antibody [NBP1-84610] - Staining of human endometrium shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/MCAF1-Antibody-Immunohistochemistry-Paraffin-NBP1-84610-img0009.jpg)
![Immunohistochemistry-Paraffin: MCAF1 Antibody [NBP1-84610] - Staining of human lymph node shows moderate nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/MCAF1-Antibody-Immunohistochemistry-Paraffin-NBP1-84610-img0010.jpg)
![Immunohistochemistry-Paraffin: MCAF1 Antibody [NBP1-84610] - Staining of human skeletal muscle shows low nuclear positivity in myocytes as expected.](http://images.novusbio.com/fullsize/MCAF1-Antibody-Immunohistochemistry-Paraffin-NBP1-84610-img0011.jpg)
![Western Blot: MCAF1 Antibody [NB100-438] - C11orf46 binds to the SETDB1 complex. Co-immunoprecipitation experiments using protein lysates from HEK293 cells overexpressing FLAG-tagged C11orf46, C11orf46delta, or C11orf46R236H showed that absence of CRD domain and R236H mutation is associated with weakened or non-detectable C11orf46-SETDB1 binding and partial loss of binding to other components. R236H mutation does not fully disrupt C11orf46-KAP1 binding. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-12013-y), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MCAF1-Antibody-Western-Blot-NB100-438-img0012.jpg "Western Blot: MCAF1 Antibody [NB100-438] - C11orf46 binds to the SETDB1 complex. Co-immunoprecipitation experiments using protein lysates from HEK293 cells overexpressing FLAG-tagged C11orf46, C11orf46delta, or C11orf46R236H showed that absence of CRD domain and R236H mutation is associated with weakened or non-detectable C11orf46-SETDB1 binding and partial loss of binding to other components. R236H mutation does not fully disrupt C11orf46-KAP1 binding. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-12013-y), licensed under a CC-BY license.")
![Immunohistochemistry: MCAF1 Antibody [NB100-438] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-MCAF used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MCAF1-Antibody-Immunohistochemistry-NB100-438-img0009.jpg)
![Western Blot: MCAF1 Antibody [NB100-438] - Nuclear extract (20 ug) from HeLa cells. Antibody used at 0.2 and 0.1 ug/ml.](http://images.novusbio.com/fullsize/MCAF1-Antibody-Western-Blot-NB100-438-img0006.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: MCAF1 Antibody [NB100-438] - Detection of Human MCAF by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCAF antibody NB100-438 used for WB at 0.05 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/MCAF1-Antibody-Western-Blot-NB100-438-img0011.jpg)
![Immunohistochemistry-Paraffin: MCAF1 Antibody [NB100-438] - FFPE section of human small cell lung cancer. NB100-438 used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit](http://images.novusbio.com/fullsize/MCAF1-Antibody-Immunohistochemistry-Paraffin-NB100-438-img0008.jpg)
![Western Blot: MCAF1 Antibody [NBP3-03759] - Western blot analysis of extracts of various cell lines, using MCAF1 antibody (NBP3-03759) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/MCAF1 Antibody-Western Blot-NBP3-03759-img0001.jpg "Western Blot: MCAF1 Antibody [NBP3-03759] - Western blot analysis of extracts of various cell lines, using MCAF1 antibody (NBP3-03759) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Western Blot: MCAF1 Antibody [NBP3-10427] - Western blot analysis of MCAF1 in Mouse Lung lysates. Antibody dilution at 1.0ug/ml](http://images.novusbio.com/fullsize/MCAF1-Antibody-Western-Blot-NBP3-10427-img0001.jpg "Western Blot: MCAF1 Antibody [NBP3-10427] - Western blot analysis of MCAF1 in Mouse Lung lysates. Antibody dilution at 1.0ug/ml")
![Immunocytochemistry/Immunofluorescence: MCAF1 Antibody [NBP2-97607] - Immunofluorescence staining of MCAF1 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human MCAF1 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/MCAF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-97607-img0001.jpg "Immunocytochemistry/Immunofluorescence: MCAF1 Antibody [NBP2-97607] - Immunofluorescence staining of MCAF1 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human MCAF1 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.")