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![Knockout Validated: ASAP1 Antibody [NBP2-48909] - Loss of ASAP1 results in expansion of the hypertrophic zone. E15.5 Asap1+/+ (Q,R) and Asap1GT/GT (S,T). ASAP1 immunostaining indicates specific expression of ASAP1 in the perichondrium, in late hypertrophic chondrocytes and in the primary ossification center. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1008216) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ASAP1-Antibody-Knockout-Validated-NBP2-48909-img0004.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: ASAP1 Antibody [NBP2-48909] - Loss of ASAP1 results in expansion of the hypertrophic zone. E15.5 Asap1+/+ (Q,R) and Asap1GT/GT (S,T). ASAP1 immunostaining indicates specific expression of ASAP1 in the perichondrium, in late hypertrophic chondrocytes and in the primary ossification center. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1008216) licensed under a CC-BY license.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ASAP1 Antibody [NBP2-48909] - Immunofluorescent staining of human cell line A-431 shows localization to centrosome.](http://images.novusbio.com/fullsize/ASAP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48909-img0003.jpg)
![Immunohistochemistry-Paraffin: ASAP1 Antibody [NBP2-48909] - Staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.](http://images.novusbio.com/fullsize/ASAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-48909-img0002.jpg)
![Immunohistochemistry: ASAP1 Antibody [NBP2-48909] - Loss of ASAP1 results in expansion of the hypertrophic zone.(A-D) E15.5 Asap1+/+ & Asap1GT/GT growth plates of the humerus stained for Alcian Blue / von Kossa in blue & black, respectively. The length of the hypertrophic zone (hz) is demarcated by a black bar. (E-T) Immunostaining of E15.5 Asap1+/+ & Asap1GT/GT humeral growth plates. Staining of the hypertrophic marker collagen X (E-H) & the osteogenic markers osteocalcin (I-L) & osteopontin (M-P) was detected by Nova Red & counterstained with hematoxilin. ASAP1 immunostaining indicates specific expression of ASAP1 in the perichondrium, in late hypertrophic chondrocytes & in the primary ossification center (Q-T). The scale bars shown at the bottom of the panels apply to all pictures above the given scale bar. (U) Quantification of the distal hypertrophic zones of the humerus of Asap1+/+ & Asap1GT/GT animals (Asap1+/+ n = 5, Asap1GT/GT n = 5). Graphs show the mean ± SD. Significance was calculated using the Student’s t-test. **, p <0.005. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31246957), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-48909_rabbit-polyclonal-asap1-antibody-31020241612221.jpg)
![Immunocytochemistry/Immunofluorescence: ASAP1 [p Tyr782] Antibody [NBP1-77933] - Analysis of phosphorylated ASAP1present in mouse NIH3T3 cells transfected with activated Src. Specific staining is not present when antibody is pre-incubated with the immunizing peptide prior to reaction with cells.](http://images.novusbio.com/fullsize/ASAP1-[p-Tyr782]-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77933-img0005.jpg "Immunocytochemistry/Immunofluorescence: ASAP1 [p Tyr782] Antibody [NBP1-77933] - Analysis of phosphorylated ASAP1present in mouse NIH3T3 cells transfected with activated Src. Specific staining is not present when antibody is pre-incubated with the immunizing peptide prior to reaction with cells.")
![Immunohistochemistry: ASAP1 [p Tyr782] Antibody [NBP1-77933] - Used at 20 g/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate intracellular positive staining in epidermal keratinocytes in human skin at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.](http://images.novusbio.com/fullsize/ASAP1-[p-Tyr782]-Antibody-Immunohistochemistry-NBP1-77933-img0004.jpg)
![Western Blot: ASAP1 Antibody [NBP2-24643] - Analysis of ASAP1 in mouse brain lysate in the 1) absence and 2) presence of immunizing peptide using NBP2-24643 at 2.0 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/ASAP1-Antibody-Western-Blot-NBP2-24643-img0001.jpg "Western Blot: ASAP1 Antibody [NBP2-24643] - Analysis of ASAP1 in mouse brain lysate in the 1) absence and 2) presence of immunizing peptide using NBP2-24643 at 2.0 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunocytochemistry/Immunofluorescence: ASAP1 Antibody [NBP1-77934] - Analysis of ASAP1present in mouse NIH3T3 cells transfected with activated Src. Specific staining is not present when antibody is pre-incubated with the immunizing peptide prior to reaction with cells.](http://images.novusbio.com/fullsize/ASAP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77934-img0002.jpg "Immunocytochemistry/Immunofluorescence: ASAP1 Antibody [NBP1-77934] - Analysis of ASAP1present in mouse NIH3T3 cells transfected with activated Src. Specific staining is not present when antibody is pre-incubated with the immunizing peptide prior to reaction with cells.")
![Western Blot: ASAP1 Antibody [NBP1-19159] - ASAP1 by Western Blot and Immunoprecipitation: [NBP1-19159]- Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. ASAP1 was also immunoprecipitated by rabbit anti-ASAP1 antibody NBP1-19158, which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/ASAP1-Antibody-Western-Blot-NBP1-19159-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: ASAP1 Antibody [NBP1-19159] - ASAP1 by Western Blot and Immunoprecipitation: [NBP1-19159]- Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. ASAP1 was also immunoprecipitated by rabbit anti-ASAP1 antibody NBP1-19158, which recognizes an upstream epitope.")
![Immunohistochemistry-Paraffin: ASAP1 Antibody [NBP2-48519] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ASAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-48519-img0001.jpg "Immunohistochemistry-Paraffin: ASAP1 Antibody [NBP2-48519] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.")
![Western Blot: ASAP1 Overexpression Lysate (Adult Normal) [NBL1-09767] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ASAP1.](http://images.novusbio.com/fullsize/ASAP1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09767-img0002.jpg "Western Blot: ASAP1 Overexpression Lysate (Adult Normal) [NBL1-09767] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ASAP1.")