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![Immunocytochemistry/Immunofluorescence: Aquaporin-2 Antibody [NB110-74682] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Aquaporin-2 Antibody NB110-74682 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Aquaporin-2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-74682-img0005.jpg "Immunocytochemistry/Immunofluorescence: Aquaporin-2 Antibody [NB110-74682] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Aquaporin-2 Antibody NB110-74682 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunocytochemistry/Immunofluorescence: Aquaporin-2 Antibody [NB110-74682] - HepG2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Aquaporin-2 Antibody NB110-74682 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Aquaporin-2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-74682-img0006.jpg)
![Western Blot: Aquaporin-2 Antibody [NB110-74682] - Detection of Aquaporin 2 in rat kidney extracts. Cortex (CX), Outer Medulla (OM), Inner Medulla Base (IMB), Inner Medulla Papilla (IMP).](http://images.novusbio.com/fullsize/Aquaporin-2-Antibody-Western-Blot-NB110-74682-img0001.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-2 Antibody [NB110-74682] - Analysis of FFPE human kidney using 1:100 conc. of Aquaporin-2 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Lumenal staining of Aquaporin-2 in scattered tubules was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Aquaporin-2-Antibody-Immunohistochemistry-Paraffin-NB110-74682-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Aquaporin-2 Antibody [NB110-74682] - Staining of mpkCCD cell line](http://images.novusbio.com/fullsize/Aquaporin-2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-74682-img0003.jpg)
![Western Blot: Aquaporin-2 [p Ser261] Antibody [NB100-61100] - Rat kidney lysate showing specific immunolabeling of the ~ 29k and 37k glycosylated form of the AQP2 protein phosphorylated at Ser261. Immunolabeling is blocked by the phospho-peptide used as antigen (peptide) but not by the corresponding dephospho-peptide (not shown).](http://images.novusbio.com/fullsize/Aquaporin-2-[p-Ser261]-Antibody-Western-Blot-NB100-61100-img0001.jpg "Western Blot: Aquaporin-2 [p Ser261] Antibody [NB100-61100] - Rat kidney lysate showing specific immunolabeling of the ~ 29k and 37k glycosylated form of the AQP2 protein phosphorylated at Ser261. Immunolabeling is blocked by the phospho-peptide used as antigen (peptide) but not by the corresponding dephospho-peptide (not shown).")
![Immunohistochemistry-Paraffin: Aquaporin-2 Antibody [NBP1-70378] - AQP2 was incubated overnight at 4C in a 1:200 dilution on human kidney and visualized with donkey anti-goat Alexa Fluor 488. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Aquaporin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-70378-img0001.jpg "Immunohistochemistry-Paraffin: Aquaporin-2 Antibody [NBP1-70378] - AQP2 was incubated overnight at 4C in a 1:200 dilution on human kidney and visualized with donkey anti-goat Alexa Fluor 488. IHC-P image submitted by a verified customer review.")
![Immunohistochemistry-Paraffin: Aquaporin-2 Antibody [NBP2-33472] - Analysis in human kidney and colon tissues. Corresponding AQP2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Aquaporin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-33472-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Aquaporin-2 Antibody [NBP2-33472] - Analysis in human kidney and colon tissues. Corresponding AQP2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Aquaporin-2 Antibody [NBP2-33472] - Staining of human prostate shows no positivity in as expected.](http://images.novusbio.com/fullsize/Aquaporin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-33472-img0003.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-2 Antibody [NBP2-33472] - Staining of human fallopian tube shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Aquaporin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-33472-img0004.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-2 Antibody [NBP2-33472] - Staining of human colon shows no cytoplasmic positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Aquaporin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-33472-img0005.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-2 Antibody [NBP2-33472] - Staining of human kidney shows moderate positivity in luminal membrane in cells in tubules.](http://images.novusbio.com/fullsize/Aquaporin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-33472-img0006.jpg)
![Western Blot: Aquaporin-2 [p Ser269] Antibody [NBP1-48314] - vasopressin (AVP) treated rat kidney lysate showing specific immunolabeling of the approx 29k and 37k glycosylated form of the AQP2 protein phosphorylated at Ser269.](http://images.novusbio.com/fullsize/Aquaporin-2-[p-Ser269]-Antibody-Western-Blot-NBP1-48314-img0001.jpg "Western Blot: Aquaporin-2 [p Ser269] Antibody [NBP1-48314] - vasopressin (AVP) treated rat kidney lysate showing specific immunolabeling of the approx 29k and 37k glycosylated form of the AQP2 protein phosphorylated at Ser269.")
![Western Blot: Aquaporin-2 [p Ser264] Antibody [NBP1-48313] - vasopressin (AVP) treated rat kidney lysate showing specific immunolabeling of the approx 29k and 37k glycosylated form of the AQP2 protein phosphorylated at Ser264.](http://images.novusbio.com/fullsize/Aquaporin-2-[p-Ser264]-Antibody-Western-Blot-NBP1-48313-img0001.jpg "Western Blot: Aquaporin-2 [p Ser264] Antibody [NBP1-48313] - vasopressin (AVP) treated rat kidney lysate showing specific immunolabeling of the approx 29k and 37k glycosylated form of the AQP2 protein phosphorylated at Ser264.")
![Western Blot: Aquaporin-2 Antibody (5B6B12) [NBP2-61669] - Analysis using AQP2 mAb against human AQP2 (AA: 149-271) recombinant protein. (Expected MW is 39.4 kDa)](http://images.novusbio.com/fullsize/Aquaporin-2-Antibody-5B6B12-Western-Blot-NBP2-61669-img0002.jpg "Western Blot: Aquaporin-2 Antibody (5B6B12) [NBP2-61669] - Analysis using AQP2 mAb against human AQP2 (AA: 149-271) recombinant protein. (Expected MW is 39.4 kDa)")
![Western Blot: Aquaporin-2 Antibody (5B6B12) [NBP2-61669] - Analysis using AQP2 mAb against HEK293 (1) and AQP2 (AA: 149-271)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Aquaporin-2-Antibody-5B6B12-Western-Blot-NBP2-61669-img0003.jpg)
![ELISA: Aquaporin-2 Antibody (5B6B12) [NBP2-61669] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Aquaporin-2-Antibody-5B6B12-ELISA-NBP2-61669-img0001.jpg)
![SDS-Page: Recombinant Human Aquaporin-2 Protein [H00000359-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-Aquaporin-2-Protein-SDS-Page-H00000359-P01-img0002.jpg "SDS-Page: Recombinant Human Aquaporin-2 Protein [H00000359-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![ELISA: Mouse Aquaporin-2 ELISA Kit (Colorimetric) [NBP2-69845] - Samples were spiked with high concentrations of Mouse Aquaporin-2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-69845_mouse-aquaporin-2-elisa-kit-colorimetric-132202416102234.jpg "ELISA: Mouse Aquaporin-2 ELISA Kit (Colorimetric) [NBP2-69845] - Samples were spiked with high concentrations of Mouse Aquaporin-2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Aquaporin-2 ELISA Kit (Colorimetric) [NBP2-69845] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-69845_mouse-aquaporin-2-elisa-kit-colorimetric-432024857497.jpg)
![ELISA: Human Aquaporin-2 ELISA Kit (Colorimetric) [NBP2-69844] - Samples were spiked with high concentrations of Human Aquaporin-2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-69844_human-aquaporin-2-elisa-kit-colorimetric-132202416232386.jpg "ELISA: Human Aquaporin-2 ELISA Kit (Colorimetric) [NBP2-69844] - Samples were spiked with high concentrations of Human Aquaporin-2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Aquaporin-2 ELISA Kit (Colorimetric) [NBP2-69844] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-69844_human-aquaporin-2-elisa-kit-colorimetric-43202495505.jpg)
![ELISA: Rat Aquaporin-2 ELISA Kit (Colorimetric) [NBP2-69847] - Samples were spiked with high concentrations of Rat Aquaporin-2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-69847_rat-aquaporin-2-elisa-kit-colorimetric-132202416204079.jpg "ELISA: Rat Aquaporin-2 ELISA Kit (Colorimetric) [NBP2-69847] - Samples were spiked with high concentrations of Rat Aquaporin-2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Aquaporin-2 ELISA Kit (Colorimetric) [NBP2-69847] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-69847_rat-aquaporin-2-elisa-kit-colorimetric-4320249115638.jpg)