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![Immunohistochemistry-Paraffin: Aquaporin-5 Antibody [NBP2-39043] - Analysis in human salivary gland and liver tissues. Corresponding AQP5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Aquaporin-5-Antibody-Immunohistochemistry-Paraffin-NBP2-39043-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Aquaporin-5 Antibody [NBP2-39043] - Analysis in human salivary gland and liver tissues. Corresponding AQP5 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Aquaporin-5 Antibody [NBP2-39043] - Staining of human cell line SiHa shows localization to plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Aquaporin-5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-39043-img0002.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-5 Antibody [NBP2-39043] - Staining of human testis shows strong membranous positivity in subset of cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Aquaporin-5-Antibody-Immunohistochemistry-Paraffin-NBP2-39043-img0010.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-5 Antibody [NBP2-39043] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Aquaporin-5-Antibody-Immunohistochemistry-Paraffin-NBP2-39043-img0007.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-5 Antibody [NBP2-39043] - Staining of human salivary gland shows strong positivity in apical membrane in glandular cells.](http://images.novusbio.com/fullsize/Aquaporin-5-Antibody-Immunohistochemistry-Paraffin-NBP2-39043-img0008.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-5 Antibody [NBP2-39043] - Staining of human pancreas shows strong positivity in apical membrane in exocrine glandular cells.](http://images.novusbio.com/fullsize/Aquaporin-5-Antibody-Immunohistochemistry-Paraffin-NBP2-39043-img0009.jpg)
![Western Blot: Aquaporin-5 Antibody [NBP2-67247] - Total protein from human USO2, NTERA-2 and HeLa cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Aquaporin 5 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Aquaporin-5-Antibody-Western-Blot-NBP2-67247-img0001.jpg "Western Blot: Aquaporin-5 Antibody [NBP2-67247] - Total protein from human USO2, NTERA-2 and HeLa cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Aquaporin 5 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Aquaporin-5 Antibody [NBP2-67247] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-AQP5 at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Aquaporin-5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-67247-img0002.jpg)
![Western Blot: Aquaporin-5 Antibody [NBP3-12286] - Antibody dilution is 1:500 in DiluObuffer, Apparent MW is 29 KDa.](http://images.novusbio.com/fullsize/Aquaporin-5-Antibody-Western-Blot-NBP3-12286-img0002.jpg "Western Blot: Aquaporin-5 Antibody [NBP3-12286] - Antibody dilution is 1:500 in DiluObuffer, Apparent MW is 29 KDa.")
![Immunohistochemistry-Paraffin: Aquaporin-5 Antibody [NBP3-12286] - Rat kidney. 1:50 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification.](http://images.novusbio.com/fullsize/Aquaporin-5-Antibody-Immunohistochemistry-Paraffin-NBP3-12286-img0001.jpg)
![Western Blot: Aquaporin-5 Antibody [NBP2-92926] - Analysis of extracts of various cell lines, using AQP5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Aquaporin-5-Antibody-Western-Blot-NBP2-92926-img0004.jpg "Western Blot: Aquaporin-5 Antibody [NBP2-92926] - Analysis of extracts of various cell lines, using AQP5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: Aquaporin-5 Antibody [NBP2-92926] - Analysis of U2OS cells using Aquaporin-5 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Aquaporin-5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92926-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Aquaporin-5 Antibody [NBP2-92926] - Analysis of NIH/3T3 cells using Aquaporin-5 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Aquaporin-5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92926-img0001.jpg)
![Immunohistochemistry: Aquaporin-5 Antibody (SR1459) [NBP3-21684] - Immunohistochemical analysis of paraffin-embedded mouse lung, using NBP3-21684.](http://images.novusbio.com/fullsize/nbp3-21684_rabbit-aquaporin-5-mab-sr1459-5720231624732.jpg "Immunohistochemistry: Aquaporin-5 Antibody (SR1459) [NBP3-21684] - Immunohistochemical analysis of paraffin-embedded mouse lung, using NBP3-21684.")
![Western Blot: Aquaporin-5 Antibody (SR1459) [NBP3-21684] - Western blot analysis using NBP3-21684 in SW480 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21684_rabbit-aquaporin-5-mab-sr1459-5720231531235.jpg)
![Western Blot: Aquaporin-5 Antibody [NBP3-02963] - Western blot analysis of extracts of various cell lines, using Aquaporin-5 antibody (NBP3-02963) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Aquaporin-5 Antibody-Western Blot-NBP3-02963-img0002.jpg "Western Blot: Aquaporin-5 Antibody [NBP3-02963] - Western blot analysis of extracts of various cell lines, using Aquaporin-5 antibody (NBP3-02963) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: Aquaporin-5 Antibody [NBP3-02963] - Immunohistochemistry of paraffin-embedded rat lung using Aquaporin-5 Rabbit pAb (NBP3-02963) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Aquaporin-5-Antibody-Immunohistochemistry-Paraffin-NBP3-02963-img0001.jpg)
![Western Blot: Aquaporin-5 Antibody (10V2Y1) [NBP3-33420] - Western blot analysis of various lysates, using Aquaporin-5 Rabbit mAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-33420_rabbit-aquaporin-5-mab-10v2y1-1012202411343346.jpg "Western Blot: Aquaporin-5 Antibody (10V2Y1) [NBP3-33420] - Western blot analysis of various lysates, using Aquaporin-5 Rabbit mAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.")
![Western Blot: Aquaporin-5 Antibody (10V2Y1) [NBP3-33420] - Western blot analysis of various lysates, using Aquaporin-5 Rabbit mAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-33420_rabbit-aquaporin-5-mab-10v2y1-101220241136016.jpg)
![Western Blot: Aquaporin-5 Antibody [NBP2-84445] - Host: Rabbit. Target Name: AQP5. Sample Type: COLO205 Whole cell lysates. Antibody Dilution: 1.0ug/mlAQP5 is supported by BioGPS gene expression data to be expressed in COLO205](http://images.novusbio.com/fullsize/Aquaporin-5-Antibody-Western-Blot-NBP2-84445-img0001.jpg "Western Blot: Aquaporin-5 Antibody [NBP2-84445] - Host: Rabbit. Target Name: AQP5. Sample Type: COLO205 Whole cell lysates. Antibody Dilution: 1.0ug/mlAQP5 is supported by BioGPS gene expression data to be expressed in COLO205")
![Western Blot: Aquaporin-5 Overexpression Lysate (Adult Normal) [NBP2-04201] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Aquaporin-5.](http://images.novusbio.com/fullsize/Aquaporin-5-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04201-img0001.jpg "Western Blot: Aquaporin-5 Overexpression Lysate (Adult Normal) [NBP2-04201] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Aquaporin-5.")
![ELISA: Human Aquaporin-5 ELISA Kit (Colorimetric) [NBP3-27225] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-27225_human-aquaporin-5-elisa-kit-colorimetric-114202410251027.png "ELISA: Human Aquaporin-5 ELISA Kit (Colorimetric) [NBP3-27225] - Standard Curve Reference")
![ELISA: Human Aquaporin-5 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31098] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-31098_human-aquaporin-5-ready-to-use-elisa-kit-colorimetric-206202415322726.png "ELISA: Human Aquaporin-5 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31098] - Standard Curve Reference")