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![Western Blot: ABAT Antibody [NBP2-21598] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ABAT antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301 was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ABAT-Antibody-Western-Blot-NBP2-21598-img0010.jpg "Western Blot: ABAT Antibody [NBP2-21598] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ABAT antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301 was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: ABAT Antibody [NBP2-21598] - HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: ABAT protein stained by ABAT antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/ABAT-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-21598-img0008.jpg)
![Immunohistochemistry-Frozen: ABAT Antibody [NBP2-21598] - Frozen sectioned adult mouse retina. Green: ABAT protein stained by ABAT antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559) diluted at 1:250. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/ABAT-Antibody-Immunohistochemistry-Frozen-NBP2-21598-img0015.jpg)
![Western Blot: ABAT Antibody [NBP2-21598] - 30 ug SK-N-SH whole cell lysate/extract 7. 5 % SDS-PAGE ABAT antibody dilution: 1:1000.](http://images.novusbio.com/fullsize/ABAT-Antibody-Western-Blot-NBP2-21598-img0001.jpg)
![Immunohistochemistry-Paraffin: ABAT Antibody [NBP2-21598] - Mouse kidney. ABAT antibody dilution: 1:500.](http://images.novusbio.com/fullsize/ABAT-Antibody-Immunohistochemistry-Paraffin-NBP2-21598-img0007.jpg)
![Immunohistochemistry-Paraffin: ABAT Antibody [NBP2-21598] - Rat brain. ABAT antibody diluted at 1:500.](http://images.novusbio.com/fullsize/ABAT-Antibody-Immunohistochemistry-Paraffin-NBP2-21598-img0011.jpg)
![Immunohistochemistry-Frozen: ABAT Antibody [NBP2-21598] - Frozen sectioned E13.5 Rat brain. Green: ABAT protein stained by ABAT antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/ABAT-Antibody-Immunohistochemistry-Frozen-NBP2-21598-img0013.jpg)
![Immunohistochemistry-Paraffin: ABAT Antibody [NBP2-21598] - ABAT antibody detects ABAT protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded mouse liver.ABAT stained by ABAT antibody (NBP2-21598) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-21598_rabbit-polyclonal-abat-antibody-910202420134032.jpg)
![Western Blot: ABAT Antibody [NBP1-83400] - Analysis using Anti-ABAT antibody NBP1-83400 (A) shows similar pattern to independent antibody NBP1-83401 (B).](http://images.novusbio.com/fullsize/ABAT-Antibody-Western-Blot-NBP1-83400-img0010.jpg "<b>Independent Antibodies Validation. </b>Western Blot: ABAT Antibody [NBP1-83400] - Analysis using Anti-ABAT antibody NBP1-83400 (A) shows similar pattern to independent antibody NBP1-83401 (B).")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: ABAT Antibody [NBP1-83400] - Staining in human liver and skeletal muscle tissues using NBP1-83400 antibody. Corresponding ABAT RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ABAT-Antibody-Immunohistochemistry-Paraffin-NBP1-83400-img0018.jpg)
![Western Blot: ABAT Antibody [NBP1-83400] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/ABAT-Antibody-Western-Blot-NBP1-83400-img0005.jpg)
![Immunohistochemistry-Paraffin: ABAT Antibody [NBP1-83400] - Staining of human cerebral cortex shows moderate positivity in neuropil.](http://images.novusbio.com/fullsize/ABAT-Antibody-Immunohistochemistry-Paraffin-NBP1-83400-img0013.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ABAT Antibody [NBP1-83400] - Staining of human cerebral cortex, kidney, liver and skeletal muscle using Anti-ABAT antibody NBP1-83400 (A) shows similar protein distribution across tissues to independent antibody NBP1-83401 (B).](http://images.novusbio.com/fullsize/ABAT-Antibody-Immunohistochemistry-Paraffin-NBP1-83400-img0014.jpg)
![Immunohistochemistry-Paraffin: ABAT Antibody [NBP1-83400] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/ABAT-Antibody-Immunohistochemistry-Paraffin-NBP1-83400-img0015.jpg)
![Immunohistochemistry-Paraffin: ABAT Antibody [NBP1-83400] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/ABAT-Antibody-Immunohistochemistry-Paraffin-NBP1-83400-img0016.jpg)
![Immunohistochemistry-Paraffin: ABAT Antibody [NBP1-83400] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/ABAT-Antibody-Immunohistochemistry-Paraffin-NBP1-83400-img0017.jpg)
![Western Blot: ABAT Antibody [NBP1-83401] - Analysis using Anti-ABAT antibody NBP1-83401 (A) shows similar pattern to independent antibody NBP1-83400 (B).](http://images.novusbio.com/fullsize/ABAT-Antibody-Western-Blot-NBP1-83401-img0009.jpg "<b>Independent Antibodies Validation. </b>Western Blot: ABAT Antibody [NBP1-83401] - Analysis using Anti-ABAT antibody NBP1-83401 (A) shows similar pattern to independent antibody NBP1-83400 (B).")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: ABAT Antibody [NBP1-83401] - Analysis in human liver and skeletal muscle tissues using NBP1-83401 antibody. Corresponding ABAT RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ABAT-Antibody-Immunohistochemistry-Paraffin-NBP1-83401-img0018.jpg)
![Immunohistochemistry-Paraffin: ABAT Antibody [NBP1-83401] - Staining of human cerebral cortex shows moderate positivity in neuropil.](http://images.novusbio.com/fullsize/ABAT-Antibody-Immunohistochemistry-Paraffin-NBP1-83401-img0012.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ABAT Antibody [NBP1-83401] - Staining of human cerebral cortex, kidney, liver and skeletal muscle using Anti-ABAT antibody NBP1-83401 (A) shows similar protein distribution across tissues to independent antibody NBP1-83400 (B).](http://images.novusbio.com/fullsize/ABAT-Antibody-Immunohistochemistry-Paraffin-NBP1-83401-img0013.jpg)
![Immunohistochemistry-Paraffin: ABAT Antibody [NBP1-83401] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/ABAT-Antibody-Immunohistochemistry-Paraffin-NBP1-83401-img0014.jpg)
![Immunohistochemistry-Paraffin: ABAT Antibody [NBP1-83401] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/ABAT-Antibody-Immunohistochemistry-Paraffin-NBP1-83401-img0015.jpg)
![Immunohistochemistry-Paraffin: ABAT Antibody [NBP1-83401] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/ABAT-Antibody-Immunohistochemistry-Paraffin-NBP1-83401-img0016.jpg)
![Western Blot: ABAT Antibody [NBP1-33066] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/ABAT-Antibody-Western-Blot-NBP1-33066-img0002.jpg "Western Blot: ABAT Antibody [NBP1-33066] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.")
![Western Blot: ABAT Antibody [NBP1-33066] - Hela cells](http://images.novusbio.com/fullsize/ABAT-Antibody-Western-Blot-NBP1-33066-img0001.jpg)
![Western Blot: ABAT Antibody [NBP1-33066] - Rat liver lysate/extract 7. 5 % SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/ABAT-Antibody-Western-Blot-NBP1-33066-img0003.jpg)
![Immunohistochemistry-Paraffin: ABAT Antibody [NBP1-33066] - ABAT antibody detects ABAT protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. ABAT antibody diluted at 1:500.](http://images.novusbio.com/fullsize/ABAT-Antibody-Immunohistochemistry-Paraffin-NBP1-33066-img0005.jpg)
![Western Blot: ABAT Antibody [NBP1-33066] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ABAT antibody (NBP1-33066) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33066_rabbit-polyclonal-abat-antibody-91020242091430.jpg)
![Western Blot: ABAT Antibody (6C9) [NBP2-45541] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ABAT.](http://images.novusbio.com/fullsize/ABAT-Antibody-6C9-Western-Blot-NBP2-45541-img0004.jpg "Western Blot: ABAT Antibody (6C9) [NBP2-45541] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ABAT.")
![Immunohistochemistry: ABAT Antibody (6C9) [NBP2-45541] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/ABAT-Antibody-6C9-Immunohistochemistry-NBP2-45541-img0003.jpg)
![Immunohistochemistry: ABAT Antibody (6C9) [NBP2-45541] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)](http://images.novusbio.com/fullsize/ABAT-Antibody-6C9-Immunohistochemistry-NBP2-45541-img0001.jpg)
![Immunohistochemistry: ABAT Antibody (6C9) [NBP2-45541] - Analysis of Carcinoma of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/ABAT-Antibody-6C9-Immunohistochemistry-NBP2-45541-img0002.jpg)
![Western Blot: ABAT Antibody (10V1K9) [NBP3-16767] - Western blot analysis of extracts of various cell lines, using GABA transaminase (ABAT) Rabbit mAb (NBP3-16767) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/ABAT-Antibody-10V1K9-Western-Blot-NBP3-16767-img0004.jpg "Western Blot: ABAT Antibody (10V1K9) [NBP3-16767] - Western blot analysis of extracts of various cell lines, using GABA transaminase (ABAT) Rabbit mAb (NBP3-16767) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: ABAT Antibody (10V1K9) [NBP3-16767] - Immunohistochemistry of paraffin-embedded mouse spinal cord using GABA transaminase (ABAT) Rabbit mAb (NBP3-16767) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ABAT-Antibody-10V1K9-Immunohistochemistry-Paraffin-NBP3-16767-img0003.jpg)
![Immunohistochemistry-Paraffin: ABAT Antibody (10V1K9) [NBP3-16767] - Immunohistochemistry of paraffin-embedded rat brain using GABA transaminase (ABAT) Rabbit mAb (NBP3-16767) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ABAT-Antibody-10V1K9-Immunohistochemistry-Paraffin-NBP3-16767-img0001.jpg)
![Immunohistochemistry-Paraffin: ABAT Antibody (10V1K9) [NBP3-16767] - Immunohistochemistry of paraffin-embedded human liver cancer using GABA transaminase (ABAT) Rabbit mAb (NBP3-16767) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ABAT-Antibody-10V1K9-Immunohistochemistry-Paraffin-NBP3-16767-img0002.jpg)
![Western Blot: ABAT Antibody (S08-9E3) [NBP3-19553] - Western blot detection of ABAT in Rat Brain lysates using NBP3-19553 (1:1000 diluted). Predicted band size: 56kDa. Observed band size: 56kDa.](http://images.novusbio.com/fullsize/ABAT-Antibody-S08-9E3-Western-Blot-NBP3-19553-img0001.jpg "Western Blot: ABAT Antibody (S08-9E3) [NBP3-19553] - Western blot detection of ABAT in Rat Brain lysates using NBP3-19553 (1:1000 diluted). Predicted band size: 56kDa. Observed band size: 56kDa.")
![Western Blot: ABAT Overexpression Lysate (Adult Normal) [NBP2-09448] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ABAT.](http://images.novusbio.com/fullsize/ABAT-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09448-img0001.jpg "Western Blot: ABAT Overexpression Lysate (Adult Normal) [NBP2-09448] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ABAT.")
![Western Blot: ABAT Overexpression Lysate (Adult Normal) [NBL1-07170] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ABAT.](http://images.novusbio.com/fullsize/ABAT-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07170-img0002.jpg "Western Blot: ABAT Overexpression Lysate (Adult Normal) [NBL1-07170] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ABAT.")
![Western Blot: ABAT Overexpression Lysate (Adult Normal) [NBL1-07169] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ABAT.](http://images.novusbio.com/fullsize/ABAT-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07169-img0002.jpg "Western Blot: ABAT Overexpression Lysate (Adult Normal) [NBL1-07169] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ABAT.")
![Immunohistochemistry: ABAT Antibody (5B6) [NBP3-26537] - Image of ABAT Antibody (5B6) diluted at 1:100 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26537_rabbit-abat-mab-5b6-26220241547563.jpg "Immunohistochemistry: ABAT Antibody (5B6) [NBP3-26537] - Image of ABAT Antibody (5B6) diluted at 1:100 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")