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![Immunocytochemistry/Immunofluorescence: TRRAP Antibody [NBP2-58076] - Staining of human cell line A-431 shows localization to nucleoplasm & the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TRRAP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58076-img0001.jpg "Immunocytochemistry/Immunofluorescence: TRRAP Antibody [NBP2-58076] - Staining of human cell line A-431 shows localization to nucleoplasm & the Golgi apparatus. Antibody staining is shown in green.")
![Western Blot: TRRAP Antibody [NBP3-15607] - Western blot analysis of extracts of various cell lines, using TRRAP antibody (NBP3-15607) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/TRRAP-Antibody-Western-Blot-NBP3-15607-img0002.jpg "Western Blot: TRRAP Antibody [NBP3-15607] - Western blot analysis of extracts of various cell lines, using TRRAP antibody (NBP3-15607) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Immunohistochemistry-Paraffin: TRRAP Antibody [NBP3-15607] - Immunohistochemistry of paraffin-embedded human esophageal cancer using TRRAP Rabbit pAb (NBP3-15607) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TRRAP-Antibody-Immunohistochemistry-Paraffin-NBP3-15607-img0001.jpg)
![Western Blot: TRRAP Antibody [NB100-58811] - Detection of Human and Mouse TRRAP on HeLa whole cell lysate using NB100-58811. TRRAP was also immunoprecipitated by rabbit anti-TRRAP antibody NB100-58810.](http://images.novusbio.com/fullsize/TRRAP-Antibody-Western-Blot-NB100-58811-img0011.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: TRRAP Antibody [NB100-58811] - Detection of Human and Mouse TRRAP on HeLa whole cell lysate using NB100-58811. TRRAP was also immunoprecipitated by rabbit anti-TRRAP antibody NB100-58810.")
![Immunoprecipitation: TRRAP Antibody [NB100-58810] - Detection of Human TRRAP by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-TRRAP antibody NB100-58810 used for IP at 3 mcg/mg lysate. TRRAP was also immunoprecipitated by rabbit anti- TRRAP antibody NB100-58811, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/TRRAP-Antibody-Western-Blot-NB100-58810-img0002.jpg "<b>Independent Antibodies Validation. </b>Immunoprecipitation: TRRAP Antibody [NB100-58810] - Detection of Human TRRAP by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-TRRAP antibody NB100-58810 used for IP at 3 mcg/mg lysate. TRRAP was also immunoprecipitated by rabbit anti- TRRAP antibody NB100-58811, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 30 seconds.")