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![Western Blot: ALS2CR3 Antibody (S390-43) [NBP2-59333] - Analysis of Rat Brain-Membrane showing detection of ~ 100 kDa TRAK2 protein using Mouse Anti-TRAK2 Monoclonal Antibody, Clone S390-43. Lane 1: MW Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug protein. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-TRAK2 Monoclonal Antibody at 1:1000 for 16 hours at 4 degrees C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~ 100 kDa.](http://images.novusbio.com/fullsize/ALS2CR3-Antibody-S390-43-Western-Blot-NBP2-59333-img0004.jpg "Western Blot: ALS2CR3 Antibody (S390-43) [NBP2-59333] - Analysis of Rat Brain-Membrane showing detection of ~ 100 kDa TRAK2 protein using Mouse Anti-TRAK2 Monoclonal Antibody, Clone S390-43. Lane 1: MW Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug protein. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-TRAK2 Monoclonal Antibody at 1:1000 for 16 hours at 4 degrees C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~ 100 kDa.")
![Immunocytochemistry/Immunofluorescence: ALS2CR3 Antibody (S390-43) [NBP2-59333] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Trak2 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm. Magnification: 60X.](http://images.novusbio.com/fullsize/ALS2CR3-Antibody-S390-43-Immunocytochemistry-Immunofluorescence-NBP2-59333-img0002.jpg)
![Western Blot: ALS2CR3 Antibody [NBP2-15370] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with TRAK2 antibody [N1N2], N-term diluted at 1:1000.](http://images.novusbio.com/fullsize/ALS2CR3-Antibody-Western-Blot-NBP2-15370-img0003.jpg "Western Blot: ALS2CR3 Antibody [NBP2-15370] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with TRAK2 antibody [N1N2], N-term diluted at 1:1000.")
![Western Blot: ALS2CR3 Antibody [NBP2-15370] - Sample (50 ug of whole cell lysate) A: mouse heart 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/ALS2CR3-Antibody-Western-Blot-NBP2-15370-img0001.jpg)
![Western Blot: ALS2CR3 Antibody [NBP2-15370] - Sample (30 ug of whole cell lysate) A: A431 B: HeLa C: HepG2 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/ALS2CR3-Antibody-Western-Blot-NBP2-15370-img0002.jpg)
![Western Blot: ALS2CR3 Antibody [NBP2-15369] - Sample (50 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize/ALS2CR3-Antibody-Western-Blot-NBP2-15369-img0001.jpg "Western Blot: ALS2CR3 Antibody [NBP2-15369] - Sample (50 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE diluted at 1:1000")
![Immunocytochemistry/Immunofluorescence: ALS2CR3 Antibody [NBP2-15369] - A375 cells were fixed in 2% paraformaldehyde/culture medium at 37- for 30 min. Green: TRAK2 protein stained by TRAK2 antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/ALS2CR3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15369-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: ALS2CR3 Antibody [NBP2-80144] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL). Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/ALS2CR3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-80144-img0002.jpg "Immunocytochemistry/Immunofluorescence: ALS2CR3 Antibody [NBP2-80144] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL). Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")
![Flow Cytometry: ALS2CR3 Antibody [NBP2-80144] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/ALS2CR3-Antibody-Flow-Cytometry-NBP2-80144-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: ALS2CR3 Antibody [NBP2-55830] - Staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.](http://images.novusbio.com/fullsize/ALS2CR3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55830-img0001.jpg "Immunocytochemistry/Immunofluorescence: ALS2CR3 Antibody [NBP2-55830] - Staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.")
![Western Blot: ALS2CR3 Antibody [NBP1-31056] - Sample (30 ug of whole cell lysate)A: Hep G2 B: Molt-4 7. 5% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/ALS2CR3-Antibody-Western-Blot-NBP1-31056-img0002.jpg "Western Blot: ALS2CR3 Antibody [NBP1-31056] - Sample (30 ug of whole cell lysate)A: Hep G2 B: Molt-4 7. 5% SDS PAGE, antibody diluted at 1:1000.")