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82 Results for "TP53, Trp53"
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MAB1746

p53 Antibody (197643) [Unconjugated]

(4 Publications)
100 μg
MAB1746
Balb/3T3 mouse embryonic fibroblast cell line was untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 6 hours. p53 was immunoprecipitated from 500 µg of cell lysate following incubation with 1-3 µg Rat Anti-Mouse p53 Monoclonal Antibody (Catalog # MAB1746) for 1 hour on ice. p53-antibody complexes were absorbed using goat anti-rat IgG sepharose (Invitrogen). Immunoprecipitated p53 was detected by Western blot using a 1:5000 dilution of Human/Mouse/Rat p53 Horseradish Peroxidase-conjugated Antigen Affinity-purified Polyclonal Antibody (HAF1355). View our recommended buffer recipes for immunoprecipitation.
Mu
Species Glossary
Reactivity: Mu
IP
Applications: IP
Rat Monoclonal
Host: Rat Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB1746-SP
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AF1355

p53 Antibody [Unconjugated]

(1 Review)
(30 Publications)
100 μg
AF1355
Western blot shows lysates of CEM human T-lymphoblastoid cell line and MCF-7 human breast cancer cell line were mock-treated (-) or exposed (+) to 10 Gy ionizing radiation (IR) and harvested after 1 hour. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat p53 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1355), followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for p53 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
WB, Simple Western, IP, ChIP, ICC/IF, KO
Applications: WB, Simple Western, IP, ChIP, ICC/IF, KO
Goat Polyclonal
Host: Goat Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
         
Validated by:          
Conjugate Catalog # Availability Size Price
Agarose GAF1355
Biotin BAF1355
HRP (Horseradish Peroxidase) HAF1355
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free HAF1355
Carrier Free AF1355-SP
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AF1043

p53 [p Ser15] Antibody [Unconjugated]

(14 Publications)
100 μg
AF1043
Western blot shows lysates of CEM human T-lymphoblastoid cell line untreated or exposed to 100 J/m2UV-C for the indicated time. PVDF membrane was probed with 0.2 µg/mL Human Phospho-p53 (S15) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1043) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-p53 (S15) was detected at approximately 53 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda-phosphatase (lambda-PPase) for 1 hour. For additional reference, duplicate lysates were probed with 1:5000 dilution Human/Mouse/Rat p53 HRP-conjugated Antigen Affinity-purified Polyclonal Antibody (lower panel, Catalog # HAF1355). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu
Species Glossary
Reactivity: Hu
WB, IHC, IP, ICC/IF
Applications: WB, IHC, IP, ICC/IF
Rabbit Polyclonal
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF1043-SP
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MAB1355

p53 Antibody (184721)

(11 Publications)
100 μg
MAB1355
Western blot shows lysates of MCF-7 human breast cancer cell line mock-treated (-) or treated (+) with 1 µM camptothecin (CPT) for 1 hour. Human p53 was immunoprecipitated using Mouse Anti-Human/Mouse/Rat p53 Monoclonal Antibody (Catalog # MAB1355). PVDF membrane was probed with HRP-conjugated Anti-Human/Mouse/Rat Polyclonal Antibody (Catalog # HAF1355). A specific band for p53 was detected at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
WB, IP, ICC/IF
Applications: WB, IP, ICC/IF
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB1355-SP
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AF1489

p53 [p Ser46] Antibody

(1 Publication)
100 μg
AF1489
Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 5 hours. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human Phospho-p53 (S46) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1489) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-p53 (S46) was detected at approximately 53 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda-phosphatase (lambda-PPase) for 30 minutes. For additional reference the membrane was stripped and reprobed for total p53 using Human/Mouse/Rat p53 HRP-conjugated Antigen Affinity-purified Polyclonal Antibody (lower panel, Catalog # HAF1355). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu
Species Glossary
Reactivity: Hu
WB, Simple Western, IHC
Applications: WB, Simple Western, IHC
Rabbit Polyclonal
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF1489-SP
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DYC1043-2

Human Total p53 DuoSet IC ELISA, 2 Plate

(6 Publications)
1 Kit (for 2 Plates)
DYC1043-2
ELISA p53 [Biotin]
Hu
Species Glossary
Reactivity: Hu
ELISA
Applications: ELISA
Conjugate Catalog # Availability Size Price
Biotin DYC1043E
Biotin DYC1043-5
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DYC1839-2

Human Phospho-p53 (S15) DuoSet IC ELISA, 2 Plate

(3 Publications)
1 Kit (for 2 Plates)
DYC1839-2
ELISA p53 [p Ser15] [Biotin]
Hu
Species Glossary
Reactivity: Hu
ELISA
Applications: ELISA
Conjugate Catalog # Availability Size Price
Biotin DYC1839E
Biotin DYC1839-5
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AF2996

p53 [p Ser392] Antibody

50 μg
AF2996
Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 5 hours. PVDF membrane was probed with 0.1 µg/mL Rabbit Anti-Human Phospho-p53 (S392) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2996) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-p53 (S392) was detected at approximately 53 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda-phosphatase (lambda-PPase) for 1 hour. For additional reference, the membrane was stripped and reprobed with 1:5000 dilution of Human/Mouse/Rat p53 HRP-conjugated Antigen Affinity-purified Polyclonal Antibody (lower panel, Catalog # HAF1355). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu
Species Glossary
Reactivity: Hu
WB, Simple Western
Applications: WB, Simple Western
Rabbit Polyclonal
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF2996-SP
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SP-452

Recombinant Human His6- (DYKDDDDK)-p53 Protein, CF

(3 Publications)
20 μg
Hu
Species Glossary
Reactivity: Hu
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AF3306

p53 [p Ser37] Antibody [Unconjugated]

50 μg
AF3306
Western blot shows p53 immunoprecipitated from lysates of MCF-7 human breast cancer cell line using Human/Mouse/Rat p53 Agarose-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # GAF1355). MCF-7 cell line was untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 5 hours. PVDF membrane was probed with 0.4 µg/mL Rabbit Anti-Human Phospho-p53 (S37) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3306) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-p53 (S37) was detected at approximately 53 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda-phosphatase (lambda-PPase) for 1 hour. For additional reference the membrane was stripped and reprobed with 1:5000 dilution of Human/Mouse/Rat p53 HRP-conjugated Antigen Affinity-purified Polyclonal Antibody (lower panel, Catalog # HAF1355) This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu
Species Glossary
Reactivity: Hu
WB
Applications: WB
Rabbit Polyclonal
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF3306-SP
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MAB13551

p53 Antibody (184727) [Unconjugated]

100 μg
MAB13551
MCF-7 cells were stained with Mouse Anti-Human p53 Monoclonal Antibody (Catalog # MAB13551, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Hu
Species Glossary
Reactivity: Hu
CyTOF-ready, Flow
Applications: CyTOF-ready, Flow
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 FAB13551U
Alexa Fluor 350 IC13551U-100UG
Alexa Fluor 405 FAB13551V
Alexa Fluor 405 IC13551V-100UG
Alexa Fluor 488 IC13551G-100UG
Alexa Fluor 488 FAB13551G
Alexa Fluor 532 FAB13551X
Alexa Fluor 594 IC13551T-100UG
Alexa Fluor 594 FAB13551T
Alexa Fluor 647 IC13551R-100UG
Alexa Fluor 647 FAB13551R
Alexa Fluor 700 FAB13551N
Alexa Fluor 700 IC13551N-100UG
Alexa Fluor 750 FAB13551S
Alexa Fluor 750 IC13551S-100UG
Allophycocyanin FAB13551A
Allophycocyanin IC13551A
Biotin FAB13551B
DyLight 350 FAB13551D
DyLight 405 FAB13551E
DyLight 488 FAB13551K
DyLight 550 FAB13551L
DyLight 594 FAB13551M
DyLight 650 FAB13551W
DyLight 680 FAB13551Y
DyLight 755 FAB13551Z
FITC FAB13551F
HRP FAB13551H
Janelia Fluor 549 FAB13551I
Janelia Fluor 646 FAB13551J
PE FAB13551P
PerCP FAB13551C
mFluor Violet 450 SE FAB13551MFV450
mFluor Violet 500 SE FAB13551MFV500
mFluor Violet 610 SE FAB13551MFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free IC13551A
Carrier Free MAB13551-SP
Carrier Free IC13551V-100UG
Carrier Free IC13551U-100UG
Carrier Free IC13551T-100UG
Carrier Free IC13551S-100UG
Carrier Free IC13551R-100UG
Carrier Free IC13551N-100UG
Carrier Free IC13551G-100UG
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MAB1489

p53 [p Ser46] Antibody (1022G) [Unconjugated]

100 μg
MAB1489
Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 1 µM Camptothecin (CPT) for 5 hours. PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human Phospho-p53 (S46) Monoclonal Antibody (Catalog # MAB1489) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for phospho-p53 (S46) at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu
Species Glossary
Reactivity: Hu
WB
Applications: WB
Rabbit Monoclonal
Host: Rabbit Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB1489-SP
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