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![Western Blot: SPDEF Antibody [NBP2-88355] - Host: Rabbit. Target Name: SPDEF. Sample Tissue: Human HepG2 Whole Cell. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/SPDEF-Antibody-Western-Blot-NBP2-88355-img0002.jpg "Western Blot: SPDEF Antibody [NBP2-88355] - Host: Rabbit. Target Name: SPDEF. Sample Tissue: Human HepG2 Whole Cell. Antibody Dilution: 1ug/ml")
![Western Blot: SPDEF Antibody [NBP2-88355] - WB Suggested Anti-SPDEF Antibody Titration: 0.2-1 ug/ml. Positive Control: Human Placenta](http://images.novusbio.com/fullsize/SPDEF-Antibody-Western-Blot-NBP2-88355-img0001.jpg)
![Western Blot: SPDEF Antibody [NBP2-88355] - Host: Rabbit. Target Name: SPDEF. Sample Tissue: Human Jurkat Whole Cell. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/SPDEF-Antibody-Western-Blot-NBP2-88355-img0003.jpg)
![Western Blot: SPDEF Antibody (4A5) [H00025803-M01] - Analysis of SPDEF expression in transfected 293T cell line by SPDEF monoclonal antibody (M01), clone 4A5.Lane 1: SPDEF transfected lysate(37.518 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/SPDEF-Antibody-4A5-Western-Blot-H00025803-M01-img0003.jpg "Western Blot: SPDEF Antibody (4A5) [H00025803-M01] - Analysis of SPDEF expression in transfected 293T cell line by SPDEF monoclonal antibody (M01), clone 4A5.Lane 1: SPDEF transfected lysate(37.518 KDa).Lane 2: Non-transfected lysate.")
![Sandwich ELISA: SPDEF Antibody (4A5) [H00025803-M01] - Detection limit for recombinant GST tagged SPDEF is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SPDEF-Antibody-4A5-Sandwich-ELISA-H00025803-M01-img0002.jpg)
![Western Blot: SPDEF Antibody [NBP3-04686] - Analysis of various lysates using SPDEF Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Negative control (NC): K-562.Exposure time: 30s.](http://images.novusbio.com/fullsize/antibody/nbp3-04686_rabbit-polyclonal-spdef-antibody-western-blot-297202482326.jpg "Western Blot: SPDEF Antibody [NBP3-04686] - Analysis of various lysates using SPDEF Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Negative control (NC): K-562.Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: SPDEF Antibody [NBP2-56499] - Staining of human cell line MCF7 shows localization to nucleus & nucleoli. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SPDEF-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56499-img0001.jpg "Immunocytochemistry/Immunofluorescence: SPDEF Antibody [NBP2-56499] - Staining of human cell line MCF7 shows localization to nucleus & nucleoli. Antibody staining is shown in green.")
![Western Blot: SPDEF Antibody [NBP1-74237] - Human Fetal Muscle Cell Lysate, concentration 1 ug/ml.](http://images.novusbio.com/fullsize/SPDEF-Antibody-Western-Blot-NBP1-74237-img0002.jpg "Western Blot: SPDEF Antibody [NBP1-74237] - Human Fetal Muscle Cell Lysate, concentration 1 ug/ml.")
![Western Blot: SPDEF Antibody [NBP2-88354] - WB Suggested Anti-SPDEF Antibody Titration: 2.5ug/ml. ELISA Titer: 1:12500. Positive Control: MCF7 cell lysateSPDEF is strongly supported by BioGPS gene expression data to be expressed in Human MCF7 cells](http://images.novusbio.com/fullsize/SPDEF-Antibody-Western-Blot-NBP2-88354-img0001.jpg "Western Blot: SPDEF Antibody [NBP2-88354] - WB Suggested Anti-SPDEF Antibody Titration: 2.5ug/ml. ELISA Titer: 1:12500. Positive Control: MCF7 cell lysateSPDEF is strongly supported by BioGPS gene expression data to be expressed in Human MCF7 cells")
![Western Blot: SPDEF Antibody [NBP2-88356] - WB Suggested Anti-SPDEF Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Hela cell lysate](http://images.novusbio.com/fullsize/SPDEF-Antibody-Western-Blot-NBP2-88356-img0001.jpg "Western Blot: SPDEF Antibody [NBP2-88356] - WB Suggested Anti-SPDEF Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Hela cell lysate")
![Western Blot: SPDEF Overexpression Lysate (Adult Normal) [NBL1-16390] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SPDEF.](http://images.novusbio.com/fullsize/SPDEF-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-16390-img0002.jpg "Western Blot: SPDEF Overexpression Lysate (Adult Normal) [NBL1-16390] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SPDEF.")
![Western Blot: SPDEF Antibody [NBP2-94491] - Analysis of extracts of HT-29 cells, using SPDEF at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time:](http://images.novusbio.com/fullsize/SPDEF-Antibody-Western-Blot-NBP2-94491-img0001.jpg "Western Blot: SPDEF Antibody [NBP2-94491] - Analysis of extracts of HT-29 cells, using SPDEF at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time:")