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![Western Blot: RUVBL2 Antibody [NBP1-40354] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-RuvBL2 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate. RuvBL2 was also immunoprecipitated by rabbit anti-RuvBL2 antibody NBP1-40355 which recognizes a downstream epitope.](http://images.novusbio.com/fullsize/RUVBL2-Antibody-Western-Blot-NBP1-40354-img0025.jpg "Western Blot: RUVBL2 Antibody [NBP1-40354] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-RuvBL2 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate. RuvBL2 was also immunoprecipitated by rabbit anti-RuvBL2 antibody NBP1-40355 which recognizes a downstream epitope.")
![Immunohistochemistry-Paraffin: RUVBL2 Antibody [NBP1-40354] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- RuvBL2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/RUVBL2-Antibody-Immunohistochemistry-Paraffin-NBP1-40354-img0026.jpg)
![Immunocytochemistry/ Immunofluorescence: RUVBL2 Antibody [NBP1-40354] - Endogenous RUVBL1 & RUVBL2 colocalize with HA-NP. HeLa cells were transfected with vector control, HA-NP, or HA-VP35. Twenty-four h later, the cells were fixed & processed for immunofluorescence detection of endogenous RUVBL1 or RUVBL2 in the presence of vector control, HA-NP, or HA-VP35. Representative images of (A) endogenous RUVBL1 localization pattern with control vector (top panels), HA-NP (middle panels), or HA-VP35 (bottom panels) & (B) endogenous RUVBL2 localization pattern with control vector (top panels), HA-NP (middle panels), or HA-VP35 (bottom panels) are shown. HA-NP or HA-VP35 (green), RUVBL1/2 (red), & Hoechst 33342 nuclear stain (blue) were visualized by confocal microscopy. Scale bars = 20 µM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31018511), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-40354_rabbit-polyclonal-ruvbl2-antibody-310202415293330.jpg)
![Western Blot: RUVBL2 Antibody [NBP1-40354] - RUVBL1/2 do not effect EBOV minigenome activity. (A) Schematic diagram of the EBOV minigenome system. The EBOV minigenome system consists of six plasmids: Four support plasmids encode replication complex components NP, L, VP35, & VP30. The EBOV minigenome plasmid encodes a firefly luciferase reporter gene flanked by the leader & trailer sequences of EBOV, & the plasmid that encodes Renilla luciferase is used for normalization. (B) Minigenome activity upon the knockdown of either RUVBL1, RUVBL2, or in combination. Below are protein levels confirmed by immunoblot. HeLa cells were transfected with 80 nM scrambled siRNA, 30 nM siRNA targeting RUVBL1, or 50 nM siRNA targeting RUVBL2. Twenty-four h after siRNA addition, the minigenome components were transfected. Forty-eight h later, minigenome reporter activity was measured. (C) Overexpression of FLAG-RUVBL1 & HA-RUVBL2 in the EBOV minigenome. HeLa cells were left untransfected, or transfected with vector control (VC), or increasing amounts of FLAG-RUVBL1 (125, 250, & 500 ng) or HA-RUVBL2 (125, 250, & 500 ng). Twenty-four h after exogenous transfection, the minigenome components were transfected. Forty-eight h later, minigenome reporter activity was measured. Data represent mean ± SEM from one representative experiment (n = 3) of at least three experiments (* p < 0.05). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31018511), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-40354_rabbit-polyclonal-ruvbl2-antibody-31020241616020.jpg)
![Western Blot: RUVBL2 Antibody (1A6) [NBP2-01764] - Analysis of extracts (10ug) from 9 Human tissue by using anti-RUVBL2 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).](http://images.novusbio.com/fullsize/RUVBL2-Antibody-1A6-Western-Blot-NBP2-01764-img0010.jpg "Western Blot: RUVBL2 Antibody (1A6) [NBP2-01764] - Analysis of extracts (10ug) from 9 Human tissue by using anti-RUVBL2 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).")
![Immunocytochemistry/Immunofluorescence: RUVBL2 Antibody (1A6) [NBP2-01764] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY RUVBL2.](http://images.novusbio.com/fullsize/RUVBL2-Antibody-1A6-Immunocytochemistry-Immunofluorescence-NBP2-01764-img0007.jpg)
![Immunohistochemistry-Paraffin: RUVBL2 Antibody (1A6) [NBP2-01764] - Staining of paraffin-embedded Human tonsil using anti-RUVBL2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RUVBL2-Antibody-1A6-Immunohistochemistry-Paraffin-NBP2-01764-img0006.jpg)
![Flow Cytometry: RUVBL2 Antibody (1A6) [NBP2-01764] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RUVBL2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/RUVBL2-Antibody-1A6-Flow-Cytometry-NBP2-01764-img0001.jpg)
![Western Blot: RUVBL2 Antibody (1A6) [NBP2-01764] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RUVBL2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RUVBL2.](http://images.novusbio.com/fullsize/RUVBL2-Antibody-1A6-Western-Blot-NBP2-01764-img0002.jpg)
![Western Blot: RUVBL2 Antibody (1A6) [NBP2-01764] Analysis of extracts (35ug) from 9 different cell lines by using anti-RUVBL2 monoclonal antibody.](http://images.novusbio.com/fullsize/RUVBL2-Antibody-1A6-Western-Blot-NBP2-01764-img0008.jpg)
![Western Blot: RUVBL2 Antibody (1A6) [NBP2-01764] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using antiRUVBL2 monoclonal antibody.(1:200)](http://images.novusbio.com/fullsize/RUVBL2-Antibody-1A6-Western-Blot-NBP2-01764-img0009.jpg)
![Immunohistochemistry-Paraffin: RUVBL2 Antibody (1A6) [NBP2-01764] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-RUVBL2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RUVBL2-Antibody-1A6-Immunohistochemistry-Paraffin-NBP2-01764-img0003.jpg)
![Immunohistochemistry-Paraffin: RUVBL2 Antibody (1A6) [NBP2-01764] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-RUVBL2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RUVBL2-Antibody-1A6-Immunohistochemistry-Paraffin-NBP2-01764-img0004.jpg)
![Immunohistochemistry-Paraffin: RUVBL2 Antibody (1A6) [NBP2-01764] - Staining of paraffin-embedded Human endometrium tissue using anti-RUVBL2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RUVBL2-Antibody-1A6-Immunohistochemistry-Paraffin-NBP2-01764-img0005.jpg)
![Western Blot: RUVBL2 Antibody [NBP1-33599] - Sample (30 ug of whole cell lysate) A: H1299 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/RUVBL2-Antibody-Western-Blot-NBP1-33599-img0004.jpg "Western Blot: RUVBL2 Antibody [NBP1-33599] - Sample (30 ug of whole cell lysate) A: H1299 10% SDS PAGE; antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: RUVBL2 Antibody [NBP1-33599] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/RUVBL2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33599-img0003.jpg)
![Immunohistochemistry-Paraffin: RUVBL2 Antibody [NBP1-33599] - Mouse testis.RUVBL2 antibody [N1C1] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/RUVBL2-Antibody-Immunohistochemistry-Paraffin-NBP1-33599-img0006.jpg)
![Immunoprecipitation: RUVBL2 Antibody [NBP1-33599] - Immunoprecipitation of RUVBL2 protein from 293T whole cell extracts using 5 ug of RUVBL2 antibody. Western blot analysis was performed using RUVBL2 antibody.](http://images.novusbio.com/fullsize/RUVBL2-Antibody-Immunoprecipitation-NBP1-33599-img0005.jpg)
![Immunohistochemistry-Paraffin: RUVBL2 Antibody [NBP2-57593] - Staining in human testis and liver tissues using anti-RUVBL2 antibody. Corresponding RUVBL2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/RUVBL2-Antibody-Immunohistochemistry-Paraffin-NBP2-57593-img0005.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: RUVBL2 Antibody [NBP2-57593] - Staining in human testis and liver tissues using anti-RUVBL2 antibody. Corresponding RUVBL2 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: RUVBL2 Antibody [NBP2-57593] - Analysis using Anti-RUVBL2 antibody NBP2-57593 (A) shows similar pattern to independent antibody NBP2-55231 (B).](http://images.novusbio.com/fullsize/RUVBL2-Antibody-Western-Blot-NBP2-57593-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: RUVBL2 Antibody [NBP2-57593] - Staining of human cell line A549 shows localization to nucleoplasm, cytosol & centrosome.](http://images.novusbio.com/fullsize/RUVBL2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57593-img0001.jpg)
![Immunohistochemistry-Paraffin: RUVBL2 Antibody [NBP2-57593] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/RUVBL2-Antibody-Immunohistochemistry-Paraffin-NBP2-57593-img0003.jpg)
![Immunohistochemistry-Paraffin: RUVBL2 Antibody [NBP2-57593] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/RUVBL2-Antibody-Immunohistochemistry-Paraffin-NBP2-57593-img0004.jpg)
![Western Blot: RUVBL2 Antibody (A1E12-R) [NBP3-32922] - Western blot analysis of RUVBL2 on different lysates with Mouse anti-RUVBL2 antibody (NBP3-32922) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: Daudi cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: SK-Br-3 cell lysate (20 ug/Lane) Lane 5: 293T cell lysate (20 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Lane 7: RAW264.7 cell lysate (20 ug/Lane) Lane 8: C6 cell lysate (20 ug/Lane) Lane 9: PC-12 cell lysate (20 ug/Lane) Lane 10: COS-1 cell lysate (20 ug/Lane) Lane 11: Mouse testis tissue lysate (40 ug/Lane) Lane 12: Rat testis tissue lysate (40 ug/Lane) Lane 13: Mouse thymus tissue lysate (40 ug/Lane) Lane 14: Rat thymus tissue lysate (40 ug/Lane) Predicted band size: 51 kDa Observed band size: 51 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32922) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32922_mouse-ruvbl2-mab-a1e12-r-28820241244297.jpg "Western Blot: RUVBL2 Antibody (A1E12-R) [NBP3-32922] - Western blot analysis of RUVBL2 on different lysates with Mouse anti-RUVBL2 antibody (NBP3-32922) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: Daudi cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: SK-Br-3 cell lysate (20 ug/Lane) Lane 5: 293T cell lysate (20 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Lane 7: RAW264.7 cell lysate (20 ug/Lane) Lane 8: C6 cell lysate (20 ug/Lane) Lane 9: PC-12 cell lysate (20 ug/Lane) Lane 10: COS-1 cell lysate (20 ug/Lane) Lane 11: Mouse testis tissue lysate (40 ug/Lane) Lane 12: Rat testis tissue lysate (40 ug/Lane) Lane 13: Mouse thymus tissue lysate (40 ug/Lane) Lane 14: Rat thymus tissue lysate (40 ug/Lane) Predicted band size: 51 kDa Observed band size: 51 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32922) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: RUVBL2 Antibody (A1E12-R) [NBP3-32922] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Mouse anti-RUVBL2 antibody (NBP3-32922) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32922) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32922_mouse-ruvbl2-mab-a1e12-r-298202414304138.jpg)
![Immunocytochemistry/ Immunofluorescence: RUVBL2 Antibody (A1E12-R) [NBP3-32922] - Immunocytochemistry analysis of HeLa cells labeling RUVBL2 with Mouse anti-RUVBL2 antibody (NBP3-32922) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-RUVBL2 antibody (NBP3-32922) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32922_mouse-ruvbl2-mab-a1e12-r-2920241192355.jpg)
![Western Blot: RUVBL2 Antibody (2B9) [NBP2-45657] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/RUVBL2-Antibody-2B9-Western-Blot-NBP2-45657-img0002.jpg "Western Blot: RUVBL2 Antibody (2B9) [NBP2-45657] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Flow Cytometry: RUVBL2 Antibody (2B9) [NBP2-45657] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by RUVBL2 antibody.](http://images.novusbio.com/fullsize/RUVBL2-Antibody-2B9-Flow-Cytometry-NBP2-45657-img0001.jpg)
![Western Blot: RUVBL2 Antibody (2B9) [NBP2-45657] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RUVBL2.](http://images.novusbio.com/fullsize/RUVBL2-Antibody-2B9-Western-Blot-NBP2-45657-img0003.jpg)
![Western Blot: RUVBL2 Antibody [NBP2-55231] - Analysis using Anti-RUVBL2 antibody NBP2-55231 (A) shows similar pattern to independent antibody NBP2-57593 (B).](http://images.novusbio.com/fullsize/RUVBL2-Antibody-Western-Blot-NBP2-55231-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: RUVBL2 Antibody [NBP2-55231] - Analysis using Anti-RUVBL2 antibody NBP2-55231 (A) shows similar pattern to independent antibody NBP2-57593 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: RUVBL2 Antibody [NBP2-55231] - Staining of human cell line U-251 MG shows localization to cytosol.](http://images.novusbio.com/fullsize/RUVBL2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55231-img0001.jpg)
![Western Blot: RUVBL2 Antibody [NBP1-33373] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 10% SDS PAGE diluted at 1:5000.](http://images.novusbio.com/fullsize/RUVBL2-Antibody-Western-Blot-NBP1-33373-img0004.jpg "Western Blot: RUVBL2 Antibody [NBP1-33373] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 10% SDS PAGE diluted at 1:5000.")
![Immunohistochemistry-Paraffin: RUVBL2 Antibody [NBP1-33373] - Paraffin-embedded Testis, using antibody 10 ug/ml.](http://images.novusbio.com/fullsize/RUVBL2-Antibody-Immunohistochemistry-Paraffin-NBP1-33373-img0003.jpg)
![Western Blot: RUVBL2 Antibody (3U4L10) [NBP3-16361] - Western blot analysis of extracts of various cell lines, using RUVBL2 Rabbit mAb (NBP3-16361) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/RUVBL2-Antibody-3U4L10-Western-Blot-NBP3-16361-img0001.jpg "Western Blot: RUVBL2 Antibody (3U4L10) [NBP3-16361] - Western blot analysis of extracts of various cell lines, using RUVBL2 Rabbit mAb (NBP3-16361) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Western Blot: RUVBL2 Antibody (3U4L10) [NBP3-16361] - Western blot analysis of extracts of various cell lines, using RUVBL2 Rabbit mAb (NBP3-16361) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/RUVBL2-Antibody-3U4L10-Western-Blot-NBP3-16361-img0002.jpg)
![Western Blot: RUVBL2 Antibody (3C6) [H00010856-M01] - RUVBL2 monoclonal antibody (M01), clone 3C6. Analysis of RUVBL2 expression in K-562.](http://images.novusbio.com/fullsize/RUVBL2-Antibody-3C6-Western-Blot-H00010856-M01-img0002.jpg "Western Blot: RUVBL2 Antibody (3C6) [H00010856-M01] - RUVBL2 monoclonal antibody (M01), clone 3C6. Analysis of RUVBL2 expression in K-562.")
![Sandwich ELISA: RUVBL2 Antibody (3C6) [H00010856-M01] - Detection limit for recombinant GST tagged RUVBL2 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RUVBL2-Antibody-3C6-Sandwich-ELISA-H00010856-M01-img0001.jpg)
![Western Blot: RUVBL2 Antibody (SR1845) [NBP3-21767] - Western blot analysis using NBP3-21767 in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21767_rabbit-ruvbl2-mab-sr1845-57202316582136.jpg "Western Blot: RUVBL2 Antibody (SR1845) [NBP3-21767] - Western blot analysis using NBP3-21767 in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate.")