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![Western Blot: OCT1 Antibody [NBP2-21584] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with OCT1 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/OCT1-Antibody-Western-Blot-NBP2-21584-img0011.jpg "Western Blot: OCT1 Antibody [NBP2-21584] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with OCT1 antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: OCT1 Antibody [NBP2-21584] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: OCT1 protein stained by OCT1 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, stained by () diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/OCT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-21584-img0006.jpg)
![Immunohistochemistry-Paraffin: OCT1 Antibody [NBP2-21584] - Rat brain. OCT1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/OCT1-Antibody-Immunohistochemistry-Paraffin-NBP2-21584-img0009.jpg)
![Immunohistochemistry-Paraffin: OCT1 Antibody [NBP2-21584] - Hela xenograft, using OCT1 antibody at 1:750 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/OCT1-Antibody-Immunohistochemistry-Paraffin-NBP2-21584-img0002.jpg)
![Immunoprecipitation: OCT1 Antibody [NBP2-21584] - 293T whole cell lysate/extract A : Control with 2.5 ug of pre-immune rabbit IgG B : Immunoprecipitation of OCT1 by 2.5 ug of OCT1 antibody 7.5% SDS-PAGE The immunoprecipitated OCT1 protein was detected by OCT1 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/OCT1-Antibody-Immunoprecipitation-NBP2-21584-img0004.jpg)
![Immunoprecipitation: OCT1 Antibody [NBP2-21584] - Immunoprecipitation of OCT1 protein from 293T whole cell extracts using 5 ug of OCT1 antibody. Western blot analysis was performed using OCT1 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/OCT1-Antibody-Immunoprecipitation-NBP2-21584-img0008.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: OCT1 Antibody [NBP2-21584] - Wild-type (WT) and OCT1 knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with OCT1 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-21584_rabbit-polyclonal-oct1-antibody-1610202419405718.jpg)
![Western Blot: OCT1 Antibody [NBP2-24677] - Western blot analysis of OCT-1 in human HL60 cell lysate using NBP2-24677 at 4 ug/ml.](http://images.novusbio.com/fullsize/OCT1-Antibody-Western-Blot-NBP2-24677-img0003.jpg "Western Blot: OCT1 Antibody [NBP2-24677] - Western blot analysis of OCT-1 in human HL60 cell lysate using NBP2-24677 at 4 ug/ml.")
![Immunocytochemistry/Immunofluorescence: OCT1 Antibody [NBP2-24677] - NTERA2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-OCT1 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/OCT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24677-img0004.jpg)
![Immunohistochemistry-Paraffin: OCT1 Antibody [NBP2-24677] - Analysis of OCT-1 in formalin-fixed, paraffin-embedded human liver tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.](http://images.novusbio.com/fullsize/OCT1-Antibody-Immunohistochemistry-Paraffin-NBP2-24677-img0001.jpg)
![Western Blot: OCT1 Antibody (8L4Y5) [NBP3-16246] - Western blot analysis of extracts of various cell lines, using POU2F1/OCT1 Rabbit mAb (NBP3-16246) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/OCT1-Antibody-8L4Y5-Western-Blot-NBP3-16246-img0003.jpg "Western Blot: OCT1 Antibody (8L4Y5) [NBP3-16246] - Western blot analysis of extracts of various cell lines, using POU2F1/OCT1 Rabbit mAb (NBP3-16246) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: OCT1 Antibody (8L4Y5) [NBP3-16246] - Immunohistochemistry of paraffin-embedded mouse liver using POU2F1/OCT1 Rabbit mAb (NBP3-16246) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/OCT1-Antibody-8L4Y5-Immunohistochemistry-Paraffin-NBP3-16246-img0002.jpg)
![Immunohistochemistry-Paraffin: OCT1 Antibody (8L4Y5) [NBP3-16246] - Immunohistochemistry of paraffin-embedded human esophageal cancer using POU2F1/OCT1 Rabbit mAb (NBP3-16246) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/OCT1-Antibody-8L4Y5-Immunohistochemistry-Paraffin-NBP3-16246-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: OCT1 Antibody [NBP2-39024] - Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/OCT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-39024-img0003.jpg "Immunocytochemistry/Immunofluorescence: OCT1 Antibody [NBP2-39024] - Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm.")
![Immunohistochemistry: OCT1 Antibody [NBP2-39024] - Staining of human lymph node shows moderate nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/OCT1-Antibody-Immunohistochemistry-NBP2-39024-img0002.jpg)
![Western Blot: OCT1 Antibody [NBP3-05865] - Lane A: K562 Whole Cell LysateLane B: Hela Whole Cell LysateLane C: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:76 kDaObserved band size:76 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/OCT1-Antibody-Western-Blot-NBP3-05865-img0002.jpg "Western Blot: OCT1 Antibody [NBP3-05865] - Lane A: K562 Whole Cell LysateLane B: Hela Whole Cell LysateLane C: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:76 kDaObserved band size:76 kDa(We are unsure as to the identity of these extra bands.)")
![Immunocytochemistry/Immunofluorescence: OCT1 Antibody [NBP3-05865] - Staining of POU2F1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human POU2F1 polyclonal antibody (1:1000) at 4? overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to nucleus.](http://images.novusbio.com/fullsize/OCT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05865-img0001.jpg)
![Western Blot: OCT1 Antibody [NBP2-92720] - Analysis of extracts of various cell lines, using 44105 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 15s.](http://images.novusbio.com/fullsize/OCT1-Antibody-Western-Blot-NBP2-92720-img0003.jpg "Western Blot: OCT1 Antibody [NBP2-92720] - Analysis of extracts of various cell lines, using 44105 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 15s.")
![Western Blot: OCT1 Antibody [NBP2-92761] - Analysis of extracts of rat kidney, using 44105 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 15s.](http://images.novusbio.com/fullsize/OCT1-Antibody-Western-Blot-NBP2-92761-img0001.jpg "Western Blot: OCT1 Antibody [NBP2-92761] - Analysis of extracts of rat kidney, using 44105 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 15s.")
![Immunocytochemistry/Immunofluorescence: OCT1 Antibody [NBP2-56190] - Staining of human cell line CACO-2 shows localization to nucleoplasm & vesicles.](http://images.novusbio.com/fullsize/OCT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56190-img0001.jpg "Immunocytochemistry/Immunofluorescence: OCT1 Antibody [NBP2-56190] - Staining of human cell line CACO-2 shows localization to nucleoplasm & vesicles.")
![ELISA: Mouse OCT1 ELISA Kit (Colorimetric) [NBP2-81191] - Samples were spiked with high concentrations of Mouse 45566 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-81191_mouse-oct1-elisa-kit-colorimetric-132202416235721.jpg "ELISA: Mouse OCT1 ELISA Kit (Colorimetric) [NBP2-81191] - Samples were spiked with high concentrations of Mouse 45566 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse OCT1 ELISA Kit (Colorimetric) [NBP2-81191] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-81191_mouse-oct1-elisa-kit-colorimetric-432024915888.jpg)
![ELISA: Human OCT1 ELISA Kit (Colorimetric) [NBP2-81190] - Samples were spiked with high concentrations of Human 45566 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-81190_human-oct1-elisa-kit-colorimetric-132202416102262.jpg "ELISA: Human OCT1 ELISA Kit (Colorimetric) [NBP2-81190] - Samples were spiked with high concentrations of Human 45566 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human OCT1 ELISA Kit (Colorimetric) [NBP2-81190] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-81190_human-oct1-elisa-kit-colorimetric-432024983561.jpg)
![Western Blot: OCT1 Overexpression Lysate (Adult Normal) [NBL1-14617] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for OCT1.](http://images.novusbio.com/fullsize/OCT1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14617-img0002.jpg "Western Blot: OCT1 Overexpression Lysate (Adult Normal) [NBL1-14617] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for OCT1.")