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![Western Blot: NEK4 Antibody [NBP1-42687] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NEK4 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. NEK4 was also immunoprecipitated by rabbit anti-NEK4 antibody NBP1-42686, which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/NEK4-Antibody-Western-Blot-NBP1-42687-img0001.jpg "<b>Independent Antibodies Validation. </b>Western Blot: NEK4 Antibody [NBP1-42687] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NEK4 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. NEK4 was also immunoprecipitated by rabbit anti-NEK4 antibody NBP1-42686, which recognizes an upstream epitope.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: NEK4 Antibody [NBP1-42687] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- NEK4 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/NEK4-Antibody-Immunohistochemistry-NBP1-42687-img0002.jpg)
![Immunohistochemistry: NEK4 Antibody [NBP1-42686] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- NEK4 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/NEK4-Antibody-Immunohistochemistry-NBP1-42686-img0003.jpg "Immunohistochemistry: NEK4 Antibody [NBP1-42686] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- NEK4 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB")
![Immunoprecipitation: NEK4 Antibody [NBP1-42686] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NEK4 antibody used for IP at 3 mcg/mg lysate. NEK4 was also immunoprecipitated by rabbit anti-NEK4 antibody NBP1-42687, which recognizes a downstream epitope.](http://images.novusbio.com/fullsize/NEK4-Antibody-Immunoprecipitation-NBP1-42686-img0002.jpg)
![Immunohistochemistry-Paraffin: NEK4 Antibody [NBP1-82528] - Staining of human testis shows cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/NEK4-Antibody-Immunohistochemistry-Paraffin-NBP1-82528-img0008.jpg "Immunohistochemistry-Paraffin: NEK4 Antibody [NBP1-82528] - Staining of human testis shows cytoplasmic positivity in Leydig cells.")
![Immunocytochemistry/Immunofluorescence: NEK4 Antibody [NBP2-68741] - Staining of human cell line U-2 OS shows localization to cytosol.](http://images.novusbio.com/fullsize/NEK4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-68741-img0001.jpg "Immunocytochemistry/Immunofluorescence: NEK4 Antibody [NBP2-68741] - Staining of human cell line U-2 OS shows localization to cytosol.")
![Western Blot: NEK4 Antibody (S09-1I1) [NBP3-15066] - Western blot detection of NEK4 in Jurkat, HepG2, Hela cell lysates using NEK4 Rabbit mAb (1:1000 diluted). Predicted band size: 95kDa. Observed band size: 95kDa.](http://images.novusbio.com/fullsize/NEK4-Antibody-S09-1I1-Western-Blot-NBP3-15066-img0001.jpg "Western Blot: NEK4 Antibody (S09-1I1) [NBP3-15066] - Western blot detection of NEK4 in Jurkat, HepG2, Hela cell lysates using NEK4 Rabbit mAb (1:1000 diluted). Predicted band size: 95kDa. Observed band size: 95kDa.")
![Sandwich ELISA: NEK4 Antibody (2E9) [H00006787-M02] - Detection limit for recombinant GST tagged NEK4 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NEK4-Antibody-2E9-Sandwich-ELISA-H00006787-M02-img0002.jpg "Sandwich ELISA: NEK4 Antibody (2E9) [H00006787-M02] - Detection limit for recombinant GST tagged NEK4 is approximately 0.3ng/ml as a capture antibody.")