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![Western Blot: NF-M Antibody [NB300-222] - Western blot analysis of different neuronal tissue and cell lysates using chicken pAb to NF-M, NB300-222, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] NIH/3T3 cells, [7] HEK293, [8] HeLa, [9] SH-SY5Y, and [10] C6 cells. Strong band at 145kDa corresponds to rodent NF-M, and about 160kDa band corresponds to human NF-M protein, visible in SHSY-5Y and HEK293 cells which have neuronal properties. NF-M is not expressed in HeLa and other cell lines tested.](http://images.novusbio.com/fullsize/NF-M-Antibody-NB300-222-img0004.jpg "Western Blot: NF-M Antibody [NB300-222] - Western blot analysis of different neuronal tissue and cell lysates using chicken pAb to NF-M, NB300-222, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] NIH/3T3 cells, [7] HEK293, [8] HeLa, [9] SH-SY5Y, and [10] C6 cells. Strong band at 145kDa corresponds to rodent NF-M, and about 160kDa band corresponds to human NF-M protein, visible in SHSY-5Y and HEK293 cells which have neuronal properties. NF-M is not expressed in HeLa and other cell lines tested.")
![Immunocytochemistry/Immunofluorescence: NF-M Antibody [NB300-222] - 160kDa Neurofilament Medium Antibody [NB300-222] - View of mixed neuron/glial cultures stained with NB300-222 (red). The NF-M protein is assembled into neurofilaments which are found throughout the axons, dendrites and perikarya of these cells.](http://images.novusbio.com/fullsize/160kDa-Neurofilament-Medium-Antibody-Immunocytochemistry-Immunofluorescence-NB300-222-img0002.jpg)
![Immunohistochemistry Free-Floating: NF-M Antibody [NB300-222] - Immunofluorescent analysis of rat cerebellum section stained with chicken pAb to NF-M, NB300-222, dilution 1:1,000 in red, and costained with mouse mAb to CNPase, dilution 1:500, in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free floating sections were stained with above antibodies. The NF-M antibody labels the axons of basket cells and other neurons, while the CNP antibody stains oligodendrocytes, cells that form the myelin sheathes around axons.](http://images.novusbio.com/fullsize/NF-M-Antibody-NB300-222-img0003.jpg)
![Knockout Validated: NF-M Antibody [NB300-133] - Analysis lysates of HeLa human cervical epithelial carcinoma parental cell line and NF-M knockout (KO) HeLa cell line. PVDF membrane was probed with 1:5,000 of Rabbit Anti-Human NF-M Polyclonal Antibody (Catalog # NB300-133) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for NF-M at approximately 155 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/NF-M-Antibody-Knockout-Validated-NB300-133-img0010.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: NF-M Antibody [NB300-133] - Analysis lysates of HeLa human cervical epithelial carcinoma parental cell line and NF-M knockout (KO) HeLa cell line. PVDF membrane was probed with 1:5,000 of Rabbit Anti-Human NF-M Polyclonal Antibody (Catalog # NB300-133) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for NF-M at approximately 155 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: NF-M Antibody [NB300-133] - Analysis of rat cerebellum section stained with rabbit pAb to NF-M, NB300-133, dilution 1:2,000 in red, and costained with mouse mAb to GAP43, dilution 1:2,000 in green. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with the above antibodies. NB300-133 antibody strongly labels neuronal processes throughout the cerebellum, while the GAP43 antibody stains predominantly synaptic regios in the molecular layer.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunocytochemistry-Immunofluorescence-NB300-133-img0008.jpg)
![Western Blot: NF-M Antibody [NB300-133] - Analysis of neuronal tissue lysates using rabbit pAb to NF-M, NB300-133, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] pig brain, [7] pig spinal cord. Strong bands at 145kDa correspond to rodent NF-M molecules, while the NF-M of pig and other larger mammals including humans run at about 160kDa.](http://images.novusbio.com/fullsize/NF-M-Antibody-Western-Blot-NB300-133-img0009.jpg)
![Immunohistochemistry-Frozen: NF-M Antibody [NB300-133] - Staining of cerebral cortex section from Rat with NF-M antibody (red) at 1:5000 dilution. This immunostaining reveals the perikarya of pyramidal neurons and dendrites as well as axons surrounding the neurons. The green channel shows staining with a monoclonal antibody to beta-adrendergic receptor kinase 1.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunohistochemistry-Frozen-NB300-133-img0007.jpg)
![Western Blot: NF-M Antibody [NB300-133] - Analysis of 160kDa Neurofilament Medium expression on whole rat cerebellum homogenate using NB300-133.](http://images.novusbio.com/fullsize/p_165_1.jpg)
![Immunocytochemistry/Immunofluorescence: NF-M Antibody [NB300-133] - Mixed neuron/glia cultures stained with NB300-133 (red) and NBP2-25141 (green), Novus' mouse monoclonal antibody to Fox1, an mRNA binding protein closely related to Fox3/NeuN. The RPCA-NF-M antibody stains axonal, dendritic and perikaryal profiles of neurons cleanly and specifically. Like antibody to Fox3/NeuN, the Fox1 antibody binds to the nuclei of neurons only. DNA is shown in blue with the DAPI stain.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunocytochemistry-Immunofluorescence-NB300-133-img0005.jpg)
![Western Blot: NF-M Antibody (3H11) [NB300-134] - Neuronal tissue lysates using mouse mAb to NF-M, dilution 1:10,000 in green: [1] protein standard (red), [2] rat spinal cord, [3] mouse spinal cord, [4] cow spinal cord, [5] rat sciatic nerve. Strong bands at ~145 kDa corresponds to rodent NF-M while that at about 160 kDa corresponds to the significantly larger bovine NF-M protein.](http://images.novusbio.com/fullsize/NF-M-Antibody-3H11-Western-Blot-NB300-134-img0004.jpg "Western Blot: NF-M Antibody (3H11) [NB300-134] - Neuronal tissue lysates using mouse mAb to NF-M, dilution 1:10,000 in green: [1] protein standard (red), [2] rat spinal cord, [3] mouse spinal cord, [4] cow spinal cord, [5] rat sciatic nerve. Strong bands at ~145 kDa corresponds to rodent NF-M while that at about 160 kDa corresponds to the significantly larger bovine NF-M protein.")
![Immunohistochemistry Free-Floating: NF-M Antibody (3H11) [NB300-134] - Adult rat frontal cortex section stained with mouse mAb to neurofilament NF-M, dilution 1:5,000 in green, and costained with chicken pAb to neurofilament NF-H, dilution 1:5,000 in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. NB300-134 antibody labels neuron cell bodies and dendrites of pyramidal neurons, as well as dendrites and axons of other neuronal cells, while the NF-H antibody stains the network of neuronal axons only.](http://images.novusbio.com/fullsize/NF-M-Antibody-3H11-Immunohistochemistry-NB300-134-img0003.jpg)
![Western Blot: 160kDa Neurofilament Medium Antibody (3H11) [NB300-134] - Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stainted with various antibodies including NB300-134 (lane 3).](http://images.novusbio.com/fullsize/160kDa-Neurofilament-Medium-Antibody-3H11-Western-Blot-NB300-134-img0002.jpg)
![Western Blot: NF-M Antibody (2688) [NBP2-46618] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/NF-M-Antibody-2688-Western-Blot-NBP2-46618-img0009.jpg "Western Blot: NF-M Antibody (2688) [NBP2-46618] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue")
![Immunohistochemistry: NF-M Antibody (2688) [NBP2-46618] - Staining of human tonsil shows absence of immunoreactivity (negative control).](http://images.novusbio.com/fullsize/NF-M-Antibody-2688-Immunohistochemistry-NBP2-46618-img0007.jpg)
![Western Blot: NF-M Antibody (2688) [NBP2-46618] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/NF-M-Antibody-2688-Western-Blot-NBP2-46618-img0008.jpg)
![Immunohistochemistry: NF-M Antibody (2688) [NBP2-46618] - Staining of rat cerebellum shows strong immunoreactivity in neuronal processes.](http://images.novusbio.com/fullsize/NF-M-Antibody-2688-Immunohistochemistry-NBP2-46618-img0001.jpg)
![Immunohistochemistry: NF-M Antibody (2688) [NBP2-46618] - Staining of mouse trigeminal ganglion shows strong immunoreactivity in ganglion cells.](http://images.novusbio.com/fullsize/NF-M-Antibody-2688-Immunohistochemistry-NBP2-46618-img0002.jpg)
![Immunohistochemistry: NF-M Antibody (2688) [NBP2-46618] - Staining of human cerebellum shows strong immunoreactivity in Purkinje cells and neural fibers.](http://images.novusbio.com/fullsize/NF-M-Antibody-2688-Immunohistochemistry-NBP2-46618-img0003.jpg)
![Immunohistochemistry: NF-M Antibody (2688) [NBP2-46618] - Staining of rat brainstem shows strong positivity in neurons of mesencephalic trigeminal nucleus.](http://images.novusbio.com/fullsize/NF-M-Antibody-2688-Immunohistochemistry-NBP2-46618-img0004.jpg)
![Immunohistochemistry: NF-M Antibody (2688) [NBP2-46618] - Staining of mouse thalamus shows strong positivity in neural fibers.](http://images.novusbio.com/fullsize/NF-M-Antibody-2688-Immunohistochemistry-NBP2-46618-img0005.jpg)
![Immunohistochemistry: NF-M Antibody (2688) [NBP2-46618] - Staining of human cerebral cortex shows strong positivity in neural fibers and cell bodies.](http://images.novusbio.com/fullsize/NF-M-Antibody-2688-Immunohistochemistry-NBP2-46618-img0006.jpg)
![Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87754] - Analysis in human cerebral cortex and liver tissues. Corresponding NF-M RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunohistochemistry-Paraffin-NBP1-87754-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87754] - Analysis in human cerebral cortex and liver tissues. Corresponding NF-M RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: NF-M Antibody [NBP1-87754] - Analysis NBP1-87754 (A) shows similar pattern to independent antibody NBP1-87753 (B).](http://images.novusbio.com/fullsize/NF-M-Antibody-Western-Blot-NBP1-87754-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: NF-M Antibody [NBP1-87754] - Staining of human cell line HEK 293 shows localization to intermediate filaments. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87754-img0010.jpg)
![Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87754] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunohistochemistry-Paraffin-NBP1-87754-img0016.jpg)
![Western Blot: NF-M Antibody [NBP1-87754] - Analysis in human cell line HEK 293 and human cell line A-431.](http://images.novusbio.com/fullsize/NF-M-Antibody-Western-Blot-NBP1-87754-img0011.jpg)
![Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87754] - Staining of human cerebellum shows strong cytoplasmic positivity in white matter.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunohistochemistry-Paraffin-NBP1-87754-img0014.jpg)
![Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87754] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunohistochemistry-Paraffin-NBP1-87754-img0015.jpg)
![Western Blot: NF-M Antibody (JM11-20) [NBP2-66978] - Analysis of NEFM on 293T cells lysates using anti-NEFM antibody at 1/500 dilution.](http://images.novusbio.com/fullsize/NF-M-Antibody-JM11-20-Western-Blot-NBP2-66978-img0003.jpg "Western Blot: NF-M Antibody (JM11-20) [NBP2-66978] - Analysis of NEFM on 293T cells lysates using anti-NEFM antibody at 1/500 dilution.")
![Immunocytochemistry/Immunofluorescence: NF-M Antibody (JM11-20) [NBP2-66978] - Staining NEFM in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NF-M-Antibody-JM11-20-Immunocytochemistry-Immunofluorescence-NBP2-66978-img0006.jpg)
![Immunohistochemistry-Paraffin: NF-M Antibody (JM11-20) [NBP2-66978] - Analysis of paraffin-embedded human brain tissue with Rabbit anti-NF-M antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/NF-M-Antibody-JM11-20-Immunohistochemistry-Paraffin-NBP2-66978-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: NF-M Antibody (JM11-20) [NBP2-66978] - Staining NEFM in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NF-M-Antibody-JM11-20-Immunocytochemistry-Immunofluorescence-NBP2-66978-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: NF-M Antibody (JM11-20) [NBP2-66978] - Staining NEFM in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NF-M-Antibody-JM11-20-Immunocytochemistry-Immunofluorescence-NBP2-66978-img0005.jpg)
![Immunohistochemistry-Paraffin: NF-M Antibody (JM11-20) [NBP2-66978] - Analysis of paraffin-embedded mouse brain tissue using anti-NEFM antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NF-M-Antibody-JM11-20-Immunohistochemistry-Paraffin-NBP2-66978-img0001.jpg)
![Immunohistochemistry-Paraffin: NF-M Antibody (JM11-20) [NBP2-66978] - Analysis of paraffin-embedded rat brain tissue using anti-NEFM antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/NF-M-Antibody-JM11-20-Immunohistochemistry-Paraffin-NBP2-66978-img0002.jpg)
![Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753] - Analysis in human cerebral cortex and endometrium tissues using NBP1-87753 antibody. Corresponding NEFM RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunohistochemistry-Paraffin-NBP1-87753-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753] - Analysis in human cerebral cortex and endometrium tissues using NBP1-87753 antibody. Corresponding NEFM RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753] - Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cell.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunohistochemistry-NBP1-87753-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: NF-M Antibody [NBP1-87753] - Analysis NBP1-87753 (A) shows similar pattern to independent antibody NBP1-87754 (B).](http://images.novusbio.com/fullsize/NF-M-Antibody-Western-Blot-NBP1-87753-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: NF-M Antibody [NBP1-87753] - Staining of human cell line HEK 293 shows localization to intermediate filaments. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87753-img0011.jpg)
![Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753] - Staining of human endometrium shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunohistochemistry-Paraffin-NBP1-87753-img0018.jpg)
![Western Blot: NF-M Antibody [NBP1-87753] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/NF-M-Antibody-Western-Blot-NBP1-87753-img0006.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: NF-M Antibody [NBP1-87753] - Analysis in human cell line HEK 293 and human cell line A-431.](http://images.novusbio.com/fullsize/NF-M-Antibody-Western-Blot-NBP1-87753-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: NF-M Antibody [NBP1-87753] - Staining of human cell line A-431 shows positivity in cytoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87753-img0004.jpg)
![Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753] - Staining of human cerebral cortex shows strong positivity in neuronal processes.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunohistochemistry-Paraffin-NBP1-87753-img0015.jpg)
![Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753] - Staining of human prostate shows strong positivity in peripheral nerve.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunohistochemistry-Paraffin-NBP1-87753-img0017.jpg)
![Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753] - Staining of human tonsil shows no positivity in lymphoid cells as expected.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunohistochemistry-Paraffin-NBP1-87753-img0019.jpg)
![Western Blot: NF-M Antibody (2678) [NBP2-46616] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/NF-M-Antibody-2678-Western-Blot-NBP2-46616-img0009.jpg "Western Blot: NF-M Antibody (2678) [NBP2-46616] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue")
![Immunohistochemistry-Paraffin: NF-M Antibody (2678) [NBP2-46616] - Staining of rat brainstem shows strong immunoreactivity in neurons of mesencephalic trigeminal nucleus.](http://images.novusbio.com/fullsize/NF-M-Antibody-2678-Immunohistochemistry-Paraffin-NBP2-46616-img0011.jpg)
![Western Blot: NF-M Antibody (2678) [NBP2-46616] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/NF-M-Antibody-2678-Western-Blot-NBP2-46616-img0008.jpg)
![Immunohistochemistry: NF-M Antibody (2678) [NBP2-46616] - Staining of mouse cerebral cortex shows strong immunoreactivity in neurofilaments.](http://images.novusbio.com/fullsize/NF-M-Antibody-2678-Immunohistochemistry-NBP2-46616-img0002.jpg)
![Immunohistochemistry: NF-M Antibody (2678) [NBP2-46616] - Staining of human cerebral cortex shows strong positivity in neural fibers.](http://images.novusbio.com/fullsize/NF-M-Antibody-2678-Immunohistochemistry-NBP2-46616-img0003.jpg)
![Immunohistochemistry: NF-M Antibody (2678) [NBP2-46616] - Staining of rat cerebellum shows strong immunoreactivity in neuronal processes.](http://images.novusbio.com/fullsize/NF-M-Antibody-2678-Immunohistochemistry-NBP2-46616-img0004.jpg)
![Immunohistochemistry: NF-M Antibody (2678) [NBP2-46616] - Staining of mouse trigeminal ganglion shows strong immunoreactivity in ganglion cells.](http://images.novusbio.com/fullsize/NF-M-Antibody-2678-Immunohistochemistry-NBP2-46616-img0005.jpg)
![Immunohistochemistry: NF-M Antibody (2678) [NBP2-46616] - Staining of human cerebellum shows strong immunoreactivity in Purkinje cells and neural fibers.](http://images.novusbio.com/fullsize/NF-M-Antibody-2678-Immunohistochemistry-NBP2-46616-img0006.jpg)
![Immunohistochemistry: NF-M Antibody (2678) [NBP2-46616] - Staining of human tonsil shows absence of immunoreactivity (negative control).](http://images.novusbio.com/fullsize/NF-M-Antibody-2678-Immunohistochemistry-NBP2-46616-img0007.jpg)
![Western Blot: NF-M Antibody (2705) [NBP2-46625] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/NF-M-Antibody-2705-Western-Blot-NBP2-46625-img0009.jpg "Western Blot: NF-M Antibody (2705) [NBP2-46625] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue")
![Immunohistochemistry: NF-M Antibody (2705) [NBP2-46625] - Staining of human liver shows absence of immunoreactivity (negative control).](http://images.novusbio.com/fullsize/NF-M-Antibody-2705-Immunohistochemistry-NBP2-46625-img0007.jpg)
![Western Blot: NF-M Antibody (2705) [NBP2-46625] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/NF-M-Antibody-2705-Western-Blot-NBP2-46625-img0008.jpg)
![Immunohistochemistry: NF-M Antibody (2705) [NBP2-46625] - Staining of rat brainstem shows strong immunoreactivity in mesencephalic trigeminal nucleus neurons.](http://images.novusbio.com/fullsize/NF-M-Antibody-2705-Immunohistochemistry-NBP2-46625-img0001.jpg)
![Immunohistochemistry: NF-M Antibody (2705) [NBP2-46625] - Staining of mouse cerebral cortex shows strong immunoreactivity in neurofilaments.](http://images.novusbio.com/fullsize/NF-M-Antibody-2705-Immunohistochemistry-NBP2-46625-img0002.jpg)
![Immunohistochemistry: NF-M Antibody (2705) [NBP2-46625] - Staining of human cerebellum shows strong immunoreactivity in Purkinje cells and neuronal processes.](http://images.novusbio.com/fullsize/NF-M-Antibody-2705-Immunohistochemistry-NBP2-46625-img0003.jpg)
![Immunohistochemistry: NF-M Antibody (2705) [NBP2-46625] - Staining of rat cerebellum shows strong immunoreactivity in neuronal processes.](http://images.novusbio.com/fullsize/NF-M-Antibody-2705-Immunohistochemistry-NBP2-46625-img0004.jpg)
![Immunohistochemistry: NF-M Antibody (2705) [NBP2-46625] - Staining of mouse trigeminal ganglion shows strong positivity in primary sensory neurons.](http://images.novusbio.com/fullsize/NF-M-Antibody-2705-Immunohistochemistry-NBP2-46625-img0005.jpg)
![Immunohistochemistry: NF-M Antibody (2705) [NBP2-46625] - Staining of human cerebral cortex shows strong positivity in neural fibers.](http://images.novusbio.com/fullsize/NF-M-Antibody-2705-Immunohistochemistry-NBP2-46625-img0006.jpg)
![Western Blot: NF-M Antibody (2697) [NBP2-46619] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/NF-M-Antibody-2697-Western-Blot-NBP2-46619-img0009.jpg "Western Blot: NF-M Antibody (2697) [NBP2-46619] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue")
![Immunohistochemistry: NF-M Antibody (2697) [NBP2-46619] - Staining of human liver shows absence of immunoreactivity (negative control).](http://images.novusbio.com/fullsize/NF-M-Antibody-2697-Immunohistochemistry-NBP2-46619-img0007.jpg)
![Western Blot: NF-M Antibody (2697) [NBP2-46619] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/NF-M-Antibody-2697-Western-Blot-NBP2-46619-img0008.jpg)
![Immunohistochemistry: NF-M Antibody (2697) [NBP2-46619] - Staining of rat cerebral cortex shows strong immunoreactivity in neuronal processes.](http://images.novusbio.com/fullsize/NF-M-Antibody-2697-Immunohistochemistry-NBP2-46619-img0001.jpg)
![Immunohistochemistry: NF-M Antibody (2697) [NBP2-46619] - Staining of mouse cerebral cortex shows strong immunoreactivity in neuronal processes.](http://images.novusbio.com/fullsize/NF-M-Antibody-2697-Immunohistochemistry-NBP2-46619-img0002.jpg)
![Immunohistochemistry: NF-M Antibody (2697) [NBP2-46619] - Staining of human cerebral cortex shows strong positivity in neural fibers.](http://images.novusbio.com/fullsize/NF-M-Antibody-2697-Immunohistochemistry-NBP2-46619-img0003.jpg)
![Immunohistochemistry: NF-M Antibody (2697) [NBP2-46619] - Staining of rat striatum shows strong positivity in neural fibers.](http://images.novusbio.com/fullsize/NF-M-Antibody-2697-Immunohistochemistry-NBP2-46619-img0004.jpg)
![Immunohistochemistry: NF-M Antibody (2697) [NBP2-46619] - Staining of mouse trigeminal ganglion shows strong positivity in primary sensory neurons.](http://images.novusbio.com/fullsize/NF-M-Antibody-2697-Immunohistochemistry-NBP2-46619-img0005.jpg)
![Immunohistochemistry: NF-M Antibody (2697) [NBP2-46619] - Staining of human cerebellum shows strong immunoreactivity in Purkinje cells and neuronal processes.](http://images.novusbio.com/fullsize/NF-M-Antibody-2697-Immunohistochemistry-NBP2-46619-img0006.jpg)
![Western Blot: NF-M Antibody (MCA-3H11) [NBP2-31206] - Analysis of neuronal tissue lysates using mouse NF-M mAb, dilution 1:10,000 (Green): [1] protein standard (red), [2] rat spinal cord, [3] mouse spinal cord, [4] cow spinal cord, [5] rat sciatic nerve. Strong bands at 145kDa correspond to rodent NF-M while that at about 160kDa corresponds to the significantly larger bovine NF-M protein.](http://images.novusbio.com/fullsize/NF-M-Antibody-MCA-3H11-Western-Blot-NBP2-31206-img0003.jpg "Western Blot: NF-M Antibody (MCA-3H11) [NBP2-31206] - Analysis of neuronal tissue lysates using mouse NF-M mAb, dilution 1:10,000 (Green): [1] protein standard (red), [2] rat spinal cord, [3] mouse spinal cord, [4] cow spinal cord, [5] rat sciatic nerve. Strong bands at 145kDa correspond to rodent NF-M while that at about 160kDa corresponds to the significantly larger bovine NF-M protein.")
![Immunohistochemistry Free-Floating: NF-M Antibody (MCA-3H11) [NBP2-31206] - Analysis of adult rat frontal cortex section stained with mouse neurofilament NF-M mAb, dilution 1:5,000 (Green), and costained with chicken neurofilament NF-H pAb, dilution 1:5,000 (Red). Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24hrs, cut to 45uM, and free-floating sections were stained with above antibodies. The mouse NF-M mAb labels neuron cell bodies and dendrites of pyramidal neurons, as well as dendrites and axons of other neuronal cells, while the chicken NF-M pAb stains the network of neuronal axons only.](http://images.novusbio.com/fullsize/NF-M-Antibody-MCA-3H11-Immunohistochemistry-Free-Floating-NBP2-31206-img0004.jpg)
![Western Blot: Neurofilament NF-M Antibody (MCA-3H11) [NBP2-31206] - Cytoskeletal homogenate of rat spinal cord was separated by 7.5% SDS-PAGE. Lane A: Western blot analysis of Neurofilament NF-M with NBP2-31206. Lane B: SDS-PAGE gel stained with Commassie Brilliant Blue. The bands corresponding to Neurofilament subunits NF-H, NF-M, NF-L, a-internexin and GFAP are indicated.](http://images.novusbio.com/fullsize/Neurofilament-NF-M-Antibody-MCA-3H11-Western-Blot-NBP2-31206-img0002.jpg)
![Western Blot: NF-M Antibody (2G3) [NBP2-46139] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NF-M.](http://images.novusbio.com/fullsize/NF-M-Antibody-2G3-Western-Blot-NBP2-46139-img0010.jpg "Western Blot: NF-M Antibody (2G3) [NBP2-46139] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NF-M.")
![Immunohistochemistry: NF-M Antibody (2G3) [NBP2-46139] - Analysis of Human testicular cancer tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/NF-M-Antibody-2G3-Immunohistochemistry-NBP2-46139-img0009.jpg)
![Immunohistochemistry: NF-M Antibody (2G3) [NBP2-46139] - Analysis of Human skin tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/NF-M-Antibody-2G3-Immunohistochemistry-NBP2-46139-img0001.jpg)
![Immunohistochemistry: NF-M Antibody (2G3) [NBP2-46139] - Analysis of Human adult heart tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/NF-M-Antibody-2G3-Immunohistochemistry-NBP2-46139-img0002.jpg)
![Immunohistochemistry: NF-M Antibody (2G3) [NBP2-46139] - Analysis of Human adult brain tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/NF-M-Antibody-2G3-Immunohistochemistry-NBP2-46139-img0003.jpg)
![Immunohistochemistry: NF-M Antibody (2G3) [NBP2-46139] - Analysis of Human melanoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/NF-M-Antibody-2G3-Immunohistochemistry-NBP2-46139-img0004.jpg)
![Immunohistochemistry: NF-M Antibody (2G3) [NBP2-46139] - Analysis of Human embryonic liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/NF-M-Antibody-2G3-Immunohistochemistry-NBP2-46139-img0005.jpg)
![Immunohistochemistry: NF-M Antibody (2G3) [NBP2-46139] - Analysis of Human muscle tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/NF-M-Antibody-2G3-Immunohistochemistry-NBP2-46139-img0006.jpg)
![Immunohistochemistry: NF-M Antibody (2G3) [NBP2-46139] - Analysis of Human embryonic brain cortex tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/NF-M-Antibody-2G3-Immunohistochemistry-NBP2-46139-img0007.jpg)
![Immunohistochemistry: NF-M Antibody (2G3) [NBP2-46139] - Analysis of Human embryonic cerebellum tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/NF-M-Antibody-2G3-Immunohistochemistry-NBP2-46139-img0008.jpg)